Journal: Molecular Therapy. Methods & Clinical Development

Article Title: Evaluation of anti-vector immune responses to adenovirus-mediated lung gene therapy and modulation by αCD20

doi: 10.1016/j.omtm.2024.101286

Figure Lengend Snippet: Single-cell sequencing identifies lung-resident T RM (A) Clonotype frequency of T cells in naive spleen (left) and lung 1 month after HAdV delivery (right). (B) Distribution of top 5 most observed clonotypes among CD4+ and CD8+ T cells. Dark puncta indicate cells that contain one of the top 5 most expanded TCR sequences. (C) UMAP gene expression clustering of CD8 T cells in naive spleen (left) and lung 1 month after HAdV delivery (right).

Article Snippet: For enumeration of peripheral blood lymphocytes, 10 μL of blood obtained by retro-orbital bleeding was stained with a 1:100 dilution of the following anti-mouse antibodies for 20 min: CD45.2 Alexa Fluor 700 (BioLegend, cat. no. 109821) or PE (BioLegend, cat. no. 109807), CD3 PE-Cy5 (BD, cat. no. 553065), CD4 PE-Cy7 (BD, cat. no. 552775), CD8 APC (BD, cat. no. 553035), CD19 BB515 (BD, cat. no. 564531).

Techniques: Sequencing, Expressing

Journal: Molecular Therapy. Methods & Clinical Development

Article Title: Evaluation of anti-vector immune responses to adenovirus-mediated lung gene therapy and modulation by αCD20

doi: 10.1016/j.omtm.2024.101286

Figure Lengend Snippet: T cell responses to adenoviral vector delivery Mice were treated as in Figure 1 . (A) Enumeration of total lung extravascular CD4+ (i.v.CD45 − CD4 + ) and CD8+ (i.v.CD45 − CD8 + ) T RM subsets. ∗ p < 0.05, ∗∗ p < 0.01 by two-way ANOVA. (B) Percentage of CD69 + CD103 + cells among i.v.CD45 − CD8 + cells. ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, ∗∗∗∗ p < 0.0001 by one-way ANOVA. (C) Enumeration of IFN-γ-secreting cells responsive to HAdV (left) and luciferase (right) peptide stimulation. ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, ∗∗∗∗ p < 0.0001 by one-way ANOVA. (D) Cytokine secretion in response to peptide stimulation. ∗∗ p < 0.01 by two-way ANOVA. Data shown are mean ± SD; n ≥ 3 mice per group; representative of 2 independent experiments.

Article Snippet: For enumeration of peripheral blood lymphocytes, 10 μL of blood obtained by retro-orbital bleeding was stained with a 1:100 dilution of the following anti-mouse antibodies for 20 min: CD45.2 Alexa Fluor 700 (BioLegend, cat. no. 109821) or PE (BioLegend, cat. no. 109807), CD3 PE-Cy5 (BD, cat. no. 553065), CD4 PE-Cy7 (BD, cat. no. 552775), CD8 APC (BD, cat. no. 553035), CD19 BB515 (BD, cat. no. 564531).

Techniques: Plasmid Preparation, Luciferase

Journal: Frontiers in Immunology

Article Title: Increased IL-23R + Th Cells Population Exhibits Higher SLEDAI-2K Scores in Systemic Lupus Erythematosus Patients

doi: 10.3389/fimmu.2021.690908

Figure Lengend Snippet: Proportion of CD3 + CD4 + Th cells, and IL-17RA + and IL-23R + Th cells subsets in SLE patients versus healthy controls. (A) Proportion of CD3 + CD4 + Th cells in SLE patients (n = 50) vs healthy controls (n = 50). (B, C) Proportion of IL-17RA + (B) and IL-23R + (C) Th cells in SLE patients vs healthy controls. The bars in each scatter plot represent the median and interquartile range.

Article Snippet: Fluorochrome-labeled antibodies for CD3 (APC-Cy7; cat no. 560176), CD4 (PE-Cy7; cat no. 557852), IL-17RA (BB700/PerCP-Cy5.5; cat no. 747946), IL-23R (FITC; cat no. FAB14001F), and isotype controls (APC-Cy7; cat no. 560167, PE-Cy7; cat no. 557872, BB700/PerCP-Cy5.5; cat no. 566404, FITC; cat no. GZ-IC0041F) (Beckton Dickinson, USA) were used at 5 μl per test whereby the antibody concentration for each test was 0.0182 μg/μl in a final volume of 55 μl, corresponding to 1 μg of antibody per test according to the protocols of the manufacturer.

Techniques: