Journal: Cellular and Molecular Life Sciences: CMLS

Article Title: Super-enhancer profiling reveals ThPOK/ZBTB7B, a CD4 + cell lineage commitment factor, as a master regulator that restricts breast cancer cells to a luminal non-migratory phenotype

doi: 10.1007/s00018-025-05913-4

Figure Lengend Snippet: T hPOK knockdown dysregulates expression of ECM, EMT and WNT/β-catenin genes and increases migration of luminal BCCs. a, b, d-f, h-i) Gene expression levels measured by qRT-PCR. Since three different shRNA against ThPOK were used, the circle for each gene is divided in thirds, one for each shRNA. The color of each third indicates the log 2 (fold-change) and the size the -log 10 (p-value), for a t -test relative to the control shRNA (shScr). Each gene was assessed in ≥ 3 biological replicates. Differential expression is shown in color scale as a log2 of the fold change, and p -values by the size of the circle as –log10. c) Bright field images show morphological changes associated with ThPOK-kd in Luminal and HER2 + cells (kd#1 was used as representative for all cell lines). Scale: each side of the square corresponds to 400 µm. g) Flow cytometry analysis showing CD44 expression in HER2 + scramble (Scr) and ThPOK-kd BT474 cells. j-k) Migration assays. j) Scratch assay showing increased migratory features of ThPOK-kd cells compared with control (scr) cells. Bar and error bars represent the mean and the SD of ≥ 3 biological replicates. *: p < 10 –6 . k) Boyden chamber assay. Bar and error bars represent the mean and the SD of ≥ 3 biological replicates, *: p < 0.05, **: p < 10 –4 . All p- values in this figure were calculated based on a two-tailed Student’s t -Test

Article Snippet: Primary antibodies: ThPOK (Novus Biologicals cat# NBP1-88077); GAPDH (Cell Signaling cat# 97166); β-Actin (Abcam cat# ab6276).

Techniques: Knockdown, Expressing, Migration, Gene Expression, Quantitative RT-PCR, shRNA, Control, Quantitative Proteomics, Flow Cytometry, Wound Healing Assay, Boyden Chamber Assay, Two Tailed Test

Journal: Cellular and Molecular Life Sciences: CMLS

Article Title: Super-enhancer profiling reveals ThPOK/ZBTB7B, a CD4 + cell lineage commitment factor, as a master regulator that restricts breast cancer cells to a luminal non-migratory phenotype

doi: 10.1007/s00018-025-05913-4

Figure Lengend Snippet: ThPOK overexpression represses ECM, EMT and WNT/β-catenin genes and decreases migration in HER2 + and TNBC BCCs. a-g) Gene expression levels measured by qRT-PCR. The log 2 (fold-change) in expression of each gene is shown in color scale and p-values by the size of the circle as –log 10 , relative to the empty vector control cells (EV). Each gene was assessed in ≥ 3 biological replicates. h) Bright field images show morphological changes associated with ThPOK overexpression in HER2 + and TNBC cells. Scale: each side of the square corresponds to 400 µm. i) Boyden chamber migration assay. Cellular migration was assessed by measuring the absorbance of crystal violet released by migrated cells in each condition. Data are expressed as a percentage relative to EV control cells. The dashed line indicates the 100%, corresponding to the migration level of EV cells. Bars and error bars represent the mean and SD of ≥ 3 biological replicates, *: p < 10 –6 . p- value was calculated based on a two-tailed Student’s t -Test

Article Snippet: Primary antibodies: ThPOK (Novus Biologicals cat# NBP1-88077); GAPDH (Cell Signaling cat# 97166); β-Actin (Abcam cat# ab6276).

Techniques: Over Expression, Migration, Gene Expression, Quantitative RT-PCR, Expressing, Plasmid Preparation, Control, Two Tailed Test

Journal: International Journal of Molecular Sciences

Article Title: Altered Expression Levels of Angiogenic Peptides in the Carotid Body of Spontaneously Hypertensive Rats

doi: 10.3390/ijms26041620

Figure Lengend Snippet: Statistical comparison of the immunostaining intensity for VEGF-A, VEGF-B, and VEGF receptors in NWRs (blue boxes) and SHRs (red boxes). Note that the immunostaining intensity for the VEGF-A in the chemosensory glomus cells of the SHR was stronger (median, 181 versus 109, *** p ≤ 0.001) than that observed in NWR rats. An enhanced and statistically significantly increased expression of vascular endothelial growth factor-B (VEGF-B) (median, 73 versus 57.5, *** p ≤ 0.001) and its receptor VEGFR2 (median, 95.5 versus 89, * p = 0.036) in glomus cells of SHR compared with NWR controls was also observed, as highlighted by the double labeling technique with their marker tyrosine hydroxylase (TH). Vascular endothelial growth factor receptor 1 (VEGFR1) is much less expressed in glomus cells of hypertensive CB compared to age-matched NWR controls (median, 79 versus 88.5, *** p ≤ 0.001).

Article Snippet: Tyrosine hydroxylase (TH) , Elabscience ® (E-AB-70206) , Monoclonal , Mouse , 1:800.

Techniques: Comparison, Immunostaining, Expressing, Labeling, Marker

Journal: International Journal of Molecular Sciences

Article Title: Altered Expression Levels of Angiogenic Peptides in the Carotid Body of Spontaneously Hypertensive Rats

doi: 10.3390/ijms26041620

Figure Lengend Snippet: List of primary and secondary antibodies used for immunofluorescence.

Article Snippet: Tyrosine hydroxylase (TH) , Elabscience ® (E-AB-70206) , Monoclonal , Mouse , 1:800.

Techniques: Immunofluorescence

Journal: Journal of Nanobiotechnology

Article Title: Edible exosome-like nanoparticles from portulaca oleracea L mitigate DSS-induced colitis via facilitating double-positive CD4 + CD8 + T cells expansion

doi: 10.1186/s12951-023-02065-0

Figure Lengend Snippet: PELNs inducing the differentiation of DP CD4 + CD8 + T cells. a , Schematic representation of mechanisms for Lactobacillus reuteri metabolizing tryptophan (L-Trp) into indole derivatives; b , PELNs promote the growth of Lactobacillus reuteri ; c , PELNs treatment significantly increased the levels of indole derivatives; d, e , PELNs treatment significantly decreased the levels of Zbtb7b protein using WB and IHC assay; f , Schematic representation for the derivatives of Lactobacillus reuteri activating the aryl hydrocarbon receptor (AhR) in conventional CD4 + T cells, and leading to the downregulation of Zbtb7b expression and the reprogramming of conventional CD4 + T cells into DP CD4 + CD8 + T cells; g , Flow cytometry detecting the population of DP CD4 + CD8 + T cells in colonic samples; h , Represent immunofluorescence showing the population of DP CD4 + CD8 + T cells in colonic samples. Data are representative FCM images or expressed as the mean ± SEM of each group. * P < 0.05, **P < 0.01

Article Snippet: An Antibodies specific to Zbtb7b were acquired from Proteintech (Cat.: 11341-1-AP, Wuhan, China), while antibodies targeting GAPDH were sourced from Affinity Biosciences (Cat.: AF7021, Jiangsu, China).

Techniques: Expressing, Flow Cytometry, Immunofluorescence