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Journal: bioRxiv
Article Title: Meningeal inflammation and arachnoid barrier breakdown in a mouse model of neonatal bacterial meningitis
doi: 10.64898/2026.03.04.709573
Figure Lengend Snippet: (A) Flow cytometry analysis of % immune cells present in LPM whole mounts between mock- and GBS-infected group. (B) % LPM CD206 + /Lyve1 + cells of live CD45 + immune cells and (C) Lyve1 mean fluorescence intensity of cells in mock- and GBS-infected group from (A). (n=5 mock, n=4 GBS for 3A-3C) (D) Representative images of L-BAMs using IF on LPM whole mounts. (E) Total CD206 + cell count and (F) Lyve1 intensity per CD206 + /Lyve1 + cell (CTCF) from (D) . (n=7 mock, n=4 GBS for 3E and 3F) Statistics: Mann-Whitney U test, * = p < 0.05, ** = p < 0.01, ns = not significant; mean and SD. Scale bar = (D) 20µm.
Article Snippet: Cells were stained with the following anti-mouse surface antibodies in MACS buffer for 30 minutes at room temperature: from BioLegend—F4/80-BV785 (clone BM8; Catalog #123141), Ultra-LEAF Purified CD16/32 (clone 93; Catalog # 101330]; from BDBiosciences—CD19-PE-Cy5 (clone 1D3; Catalog # 558079),
Techniques: Flow Cytometry, Infection, Fluorescence, Cell Characterization, MANN-WHITNEY