antibodies ebf3 Search Results


anti-ebf3 polyclonal antibody  (TargetMol)
N/A
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anti ebf3  (R&D Systems)
90
R&D Systems anti ebf3
Anti Ebf3, supplied by R&D Systems, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/antibodies+ebf3/Human%2FMouse+EBF-3+Antibody/pmc07240299-196-24-26
Average 90 stars, based on 1 article reviews
anti ebf3 - by Bioz Stars, 2026-10
90/100 stars
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ebf3 igg  (Novus Biologicals)
92
Novus Biologicals ebf3 igg
Validation of VMP- and SU-specific transcript expression in female and male P0 rat tissues. Quantitative real-time PCR (qPCR) showed significantly elevated levels of both control (Fgf10, Ptn and Scube1) and candidate <t>(Ebf3,</t> Gfra3, Nmur2, Rspo2, Scara5, Slc26a7, Robo1 and Meis2) VMP-specific transcripts versus SU. Fgf10, Ptn, Scube1, Ebf3, Gfra3, Scara5 and Meis2 were expressed in VP and DP, while Rspo2, Nmur2 and Slc26a7 showed low expression in VP and DP. SU candidate transcripts Anxa1, Enpp2 and Unc5b were enriched versus VMP. Data is represented as mean fold difference to VMP ± SD of duplicate biological replicates and duplicate technical replicates (n = 4). Significance was detected using One-way ANOVA with TUKEY multiple comparison *p < 0.05. Figures in red indicate fold difference compared to VMP.
Ebf3 Igg, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/antibodies+ebf3/EBF-3+Antibody+(8D6)/pmc05703996-248-26-32
Average 92 stars, based on 1 article reviews
ebf3 igg - by Bioz Stars, 2026-10
92/100 stars
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ebf3  (Aviva Systems)
85
Aviva Systems ebf3
Figure 1. Interaction between frequently methylated genes and TGF-β pathway components in HNSCC. A, immunoprecipitation was done with SMAD2 antibody using lysates from WT SCC22B and SCC22B + IRX1 overexpression. This revealed no IRX1 association with SMAD2. B, chromatin immunoprecipitation analysis using <t>EBF3</t> and SMAD2 antibodies in SCC22B cells. p21 and IRX1 promoter pulldown was assessed through quantitative PCR, which revealed increased pulldown of both promoters with ther respective antibody compared with the negative IgG control. C, immunoprecipitation analysis of SLC5A8 pulldown by survivin antibody. Arrow, SLC5A8, which is only pulled down by survivin and not by the IgG control. D, overexpression of SEPT9 in SCC22B and immunoprecipitation analysis of SEPT9 pulldown by HIF-1α antibody. HIF-1α associates with SEPT9, and this binding is increased in SEPT9-overexpressing cells (+SEPT9). Furthermore, SEPT9 expression correlates with decreased HIF-1α expression.
Ebf3, supplied by Aviva Systems, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/antibodies+ebf3/EBF3+antibody+-+middle+region+(ARP31837_T100)/10__1158_slash_0008___5472__can___09___3073-33-24-25
Average 85 stars, based on 1 article reviews
ebf3 - by Bioz Stars, 2026-10
85/100 stars
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antibodies ebf3  (Novus Biologicals)
92
Novus Biologicals antibodies ebf3
Figure 1. Interaction between frequently methylated genes and TGF-β pathway components in HNSCC. A, immunoprecipitation was done with SMAD2 antibody using lysates from WT SCC22B and SCC22B + IRX1 overexpression. This revealed no IRX1 association with SMAD2. B, chromatin immunoprecipitation analysis using <t>EBF3</t> and SMAD2 antibodies in SCC22B cells. p21 and IRX1 promoter pulldown was assessed through quantitative PCR, which revealed increased pulldown of both promoters with ther respective antibody compared with the negative IgG control. C, immunoprecipitation analysis of SLC5A8 pulldown by survivin antibody. Arrow, SLC5A8, which is only pulled down by survivin and not by the IgG control. D, overexpression of SEPT9 in SCC22B and immunoprecipitation analysis of SEPT9 pulldown by HIF-1α antibody. HIF-1α associates with SEPT9, and this binding is increased in SEPT9-overexpressing cells (+SEPT9). Furthermore, SEPT9 expression correlates with decreased HIF-1α expression.
Antibodies Ebf3, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/antibodies+ebf3/EBF-3+Antibody+(8D6)/pm38479556-108-9-13
Average 92 stars, based on 1 article reviews
antibodies ebf3 - by Bioz Stars, 2026-10
92/100 stars
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ebf3 antibody gtx117656  (GeneTex)
90
GeneTex ebf3 antibody gtx117656
Figure 1. Interaction between frequently methylated genes and TGF-β pathway components in HNSCC. A, immunoprecipitation was done with SMAD2 antibody using lysates from WT SCC22B and SCC22B + IRX1 overexpression. This revealed no IRX1 association with SMAD2. B, chromatin immunoprecipitation analysis using <t>EBF3</t> and SMAD2 antibodies in SCC22B cells. p21 and IRX1 promoter pulldown was assessed through quantitative PCR, which revealed increased pulldown of both promoters with ther respective antibody compared with the negative IgG control. C, immunoprecipitation analysis of SLC5A8 pulldown by survivin antibody. Arrow, SLC5A8, which is only pulled down by survivin and not by the IgG control. D, overexpression of SEPT9 in SCC22B and immunoprecipitation analysis of SEPT9 pulldown by HIF-1α antibody. HIF-1α associates with SEPT9, and this binding is increased in SEPT9-overexpressing cells (+SEPT9). Furthermore, SEPT9 expression correlates with decreased HIF-1α expression.
Ebf3 Antibody Gtx117656, supplied by GeneTex, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/antibodies+ebf3/ebf3+antibody+gtx117656/pm38479556-149-0-5
Average 90 stars, based on 1 article reviews
ebf3 antibody gtx117656 - by Bioz Stars, 2026-10
90/100 stars
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ebf3 antibody (hrp)  (biorbyt)
N/A
Rabbit polyclonal antibody against EBF3 conjugated to HRP Isotype Note: IgG Host Note: Rabbit Conjugation Note: HRP Reactivity Note: Human Application Note: ELISA
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human/mouse ebf-3 antibody  (R&D Systems)
N/A
The Human/Mouse EBF-3 Antibody from R&D Systems is a EBF-3 antibody to EBF-3. This antibody reacts with Human, Mouse. The EBF-3 antibody has been validated for the following applications: Western Blot.
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ebf3 rabbit polyclonal antibody  (OriGene)
N/A
EBF3 rabbit polyclonal antibody
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rabbit anti-human ebf3 polyclonal antibody  (Cusabio)
N/A
Rabbit anti-Human EBF3 Polyclonal Antibody
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ebf3 antibody (biotin)  (biorbyt)
N/A
Rabbit polyclonal antibody against EBF3 conjugated to Biotin Isotype Note: IgG Host Note: Rabbit Conjugation Note: Biotin Reactivity Note: Human Application Note: ELISA
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ebf 3 antibody  (Novus Biologicals)
N/A
The EBF 3 Antibody from Novus Biologicals is a rabbit polyclonal antibody to EBF 3 This antibody reacts with rat The EBF 3 Antibody has been validated for the following applications Western Blot
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Image Search Results


Validation of VMP- and SU-specific transcript expression in female and male P0 rat tissues. Quantitative real-time PCR (qPCR) showed significantly elevated levels of both control (Fgf10, Ptn and Scube1) and candidate (Ebf3, Gfra3, Nmur2, Rspo2, Scara5, Slc26a7, Robo1 and Meis2) VMP-specific transcripts versus SU. Fgf10, Ptn, Scube1, Ebf3, Gfra3, Scara5 and Meis2 were expressed in VP and DP, while Rspo2, Nmur2 and Slc26a7 showed low expression in VP and DP. SU candidate transcripts Anxa1, Enpp2 and Unc5b were enriched versus VMP. Data is represented as mean fold difference to VMP ± SD of duplicate biological replicates and duplicate technical replicates (n = 4). Significance was detected using One-way ANOVA with TUKEY multiple comparison *p < 0.05. Figures in red indicate fold difference compared to VMP.

Journal: Scientific Reports

Article Title: Identification of genes expressed in a mesenchymal subset regulating prostate organogenesis using tissue and single cell transcriptomics

doi: 10.1038/s41598-017-16685-8

Figure Lengend Snippet: Validation of VMP- and SU-specific transcript expression in female and male P0 rat tissues. Quantitative real-time PCR (qPCR) showed significantly elevated levels of both control (Fgf10, Ptn and Scube1) and candidate (Ebf3, Gfra3, Nmur2, Rspo2, Scara5, Slc26a7, Robo1 and Meis2) VMP-specific transcripts versus SU. Fgf10, Ptn, Scube1, Ebf3, Gfra3, Scara5 and Meis2 were expressed in VP and DP, while Rspo2, Nmur2 and Slc26a7 showed low expression in VP and DP. SU candidate transcripts Anxa1, Enpp2 and Unc5b were enriched versus VMP. Data is represented as mean fold difference to VMP ± SD of duplicate biological replicates and duplicate technical replicates (n = 4). Significance was detected using One-way ANOVA with TUKEY multiple comparison *p < 0.05. Figures in red indicate fold difference compared to VMP.

Article Snippet: Immunostaining of Ebf3 and Meis2 on serial sections of female and male rat P0 urogenital sinus tissue (isolated as per ) was performed as per using Ebf3 IgG (Clone 8D6, mouse monoclonal, Novus Biologicals, Littleton, Colorado, USA; dilution 1:1000) and Meis2 IgG (Clone 63-T, mouse monoclonal, Santa Cruz Biotechnology, Santa Cruz, USA; dilution 1:750).

Techniques: Biomarker Discovery, Expressing, Real-time Polymerase Chain Reaction, Control, Comparison

Immunohistochemistry of Ebf3 and Meis2 in female VMP and male prostate. To examine the distribution of Ebf3 and Meis2 in mesenchymal subsets in VMP and ventral prostate (VP) we performed immunohistochemistry using P0 rat female and male reproductive tracts. Panels ( a–d ) show Ebf3 expression; in female ( a and c ) and male ( b and d ). Ebf3 showed a highly selective distribution within VMP and VP mesenchyme, and was absent from smooth muscle (SM) and the urethra ( c , a and b ). Panels ( e–h ) show Meis2 distribution; in female ( e and g ) and male ( f and h ) mesenchyme. Urethral epithelia (URE) and prostatic epithelia (E) were negative for both Ebf3 and Meis2. Scale bars ( a , b , e and f ) = 600 µm. Scale bars ( c , d , g and h ) = 300 µm.

Journal: Scientific Reports

Article Title: Identification of genes expressed in a mesenchymal subset regulating prostate organogenesis using tissue and single cell transcriptomics

doi: 10.1038/s41598-017-16685-8

Figure Lengend Snippet: Immunohistochemistry of Ebf3 and Meis2 in female VMP and male prostate. To examine the distribution of Ebf3 and Meis2 in mesenchymal subsets in VMP and ventral prostate (VP) we performed immunohistochemistry using P0 rat female and male reproductive tracts. Panels ( a–d ) show Ebf3 expression; in female ( a and c ) and male ( b and d ). Ebf3 showed a highly selective distribution within VMP and VP mesenchyme, and was absent from smooth muscle (SM) and the urethra ( c , a and b ). Panels ( e–h ) show Meis2 distribution; in female ( e and g ) and male ( f and h ) mesenchyme. Urethral epithelia (URE) and prostatic epithelia (E) were negative for both Ebf3 and Meis2. Scale bars ( a , b , e and f ) = 600 µm. Scale bars ( c , d , g and h ) = 300 µm.

Article Snippet: Immunostaining of Ebf3 and Meis2 on serial sections of female and male rat P0 urogenital sinus tissue (isolated as per ) was performed as per using Ebf3 IgG (Clone 8D6, mouse monoclonal, Novus Biologicals, Littleton, Colorado, USA; dilution 1:1000) and Meis2 IgG (Clone 63-T, mouse monoclonal, Santa Cruz Biotechnology, Santa Cruz, USA; dilution 1:750).

Techniques: Immunohistochemistry, Expressing

Summary of primers used for qPCR.

Journal: Scientific Reports

Article Title: Identification of genes expressed in a mesenchymal subset regulating prostate organogenesis using tissue and single cell transcriptomics

doi: 10.1038/s41598-017-16685-8

Figure Lengend Snippet: Summary of primers used for qPCR.

Article Snippet: Immunostaining of Ebf3 and Meis2 on serial sections of female and male rat P0 urogenital sinus tissue (isolated as per ) was performed as per using Ebf3 IgG (Clone 8D6, mouse monoclonal, Novus Biologicals, Littleton, Colorado, USA; dilution 1:1000) and Meis2 IgG (Clone 63-T, mouse monoclonal, Santa Cruz Biotechnology, Santa Cruz, USA; dilution 1:750).

Techniques:

Figure 1. Interaction between frequently methylated genes and TGF-β pathway components in HNSCC. A, immunoprecipitation was done with SMAD2 antibody using lysates from WT SCC22B and SCC22B + IRX1 overexpression. This revealed no IRX1 association with SMAD2. B, chromatin immunoprecipitation analysis using EBF3 and SMAD2 antibodies in SCC22B cells. p21 and IRX1 promoter pulldown was assessed through quantitative PCR, which revealed increased pulldown of both promoters with ther respective antibody compared with the negative IgG control. C, immunoprecipitation analysis of SLC5A8 pulldown by survivin antibody. Arrow, SLC5A8, which is only pulled down by survivin and not by the IgG control. D, overexpression of SEPT9 in SCC22B and immunoprecipitation analysis of SEPT9 pulldown by HIF-1α antibody. HIF-1α associates with SEPT9, and this binding is increased in SEPT9-overexpressing cells (+SEPT9). Furthermore, SEPT9 expression correlates with decreased HIF-1α expression.

Journal: Cancer Research

Article Title: Disruption of Transforming Growth Factor-β Signaling by Five Frequently Methylated Genes Leads to Head and Neck Squamous Cell Carcinoma Pathogenesis

doi: 10.1158/0008-5472.can-09-3073

Figure Lengend Snippet: Figure 1. Interaction between frequently methylated genes and TGF-β pathway components in HNSCC. A, immunoprecipitation was done with SMAD2 antibody using lysates from WT SCC22B and SCC22B + IRX1 overexpression. This revealed no IRX1 association with SMAD2. B, chromatin immunoprecipitation analysis using EBF3 and SMAD2 antibodies in SCC22B cells. p21 and IRX1 promoter pulldown was assessed through quantitative PCR, which revealed increased pulldown of both promoters with ther respective antibody compared with the negative IgG control. C, immunoprecipitation analysis of SLC5A8 pulldown by survivin antibody. Arrow, SLC5A8, which is only pulled down by survivin and not by the IgG control. D, overexpression of SEPT9 in SCC22B and immunoprecipitation analysis of SEPT9 pulldown by HIF-1α antibody. HIF-1α associates with SEPT9, and this binding is increased in SEPT9-overexpressing cells (+SEPT9). Furthermore, SEPT9 expression correlates with decreased HIF-1α expression.

Article Snippet: The antibodies used in the study were as follows: IL-6 (Abcam, ab6672), ARTS (Sigma-Aldrich, A4471), SLC5A8 (Santa Cruz, sc-34189), SEPT9 (aka MSF; Abgent, AP6215a), EBF3 (Aviva Systems Biology, ARP31837_T100), survivin (Santa Cruz, sc-17779), α-tubulin (Cell Signaling Technologies), TGF-β1 (Santa Cruz, sc-146), caspase-3 (Cell Signaling, 9662), actin (Santa Cruz, sc-8432), HIF-1α (Cell Signaling, 3716), and involucrin (Aviva Systems Biology, ARP48675_P050).

Techniques: Methylation, Immunoprecipitation, Over Expression, Chromatin Immunoprecipitation, Real-time Polymerase Chain Reaction, Control, Binding Assay, Expressing

Figure 3. EBF3 regulates p21 gene expression. A, luciferase analysis of p21 promoter activity in SCC22B cells with and without EBF3 overexpression. Overexpression of EBF3 significantly increases p21 promoter activity. *, P < 0.0006. B, quantitative RT-PCR analysis of p21 mRNA expression in SCC22B cells with and without EBF3 overexpression. EBF3 overexpression results in a significant increase in p21 transcription. *, P < 0.05.

Journal: Cancer Research

Article Title: Disruption of Transforming Growth Factor-β Signaling by Five Frequently Methylated Genes Leads to Head and Neck Squamous Cell Carcinoma Pathogenesis

doi: 10.1158/0008-5472.can-09-3073

Figure Lengend Snippet: Figure 3. EBF3 regulates p21 gene expression. A, luciferase analysis of p21 promoter activity in SCC22B cells with and without EBF3 overexpression. Overexpression of EBF3 significantly increases p21 promoter activity. *, P < 0.0006. B, quantitative RT-PCR analysis of p21 mRNA expression in SCC22B cells with and without EBF3 overexpression. EBF3 overexpression results in a significant increase in p21 transcription. *, P < 0.05.

Article Snippet: The antibodies used in the study were as follows: IL-6 (Abcam, ab6672), ARTS (Sigma-Aldrich, A4471), SLC5A8 (Santa Cruz, sc-34189), SEPT9 (aka MSF; Abgent, AP6215a), EBF3 (Aviva Systems Biology, ARP31837_T100), survivin (Santa Cruz, sc-17779), α-tubulin (Cell Signaling Technologies), TGF-β1 (Santa Cruz, sc-146), caspase-3 (Cell Signaling, 9662), actin (Santa Cruz, sc-8432), HIF-1α (Cell Signaling, 3716), and involucrin (Aviva Systems Biology, ARP48675_P050).

Techniques: Gene Expression, Luciferase, Activity Assay, Over Expression, Quantitative RT-PCR, Expressing

Figure 4. Functional relevance of the interactions between the methylated candidates and components of the TGF-β pathway. A, Western blot analysis of ARTS, caspase-3, TGF-β, and involucrin expression in the five overexpressing cell lines (tubulin serves as a loading control). B, cell cycle analysis of the five overexpressing cell lines revealed a significant decrease in mitosis in IRX1-, EBF3-, and SLC5A8-overexpressing cells. *, P < 0.05; **, P < 0.01.

Journal: Cancer Research

Article Title: Disruption of Transforming Growth Factor-β Signaling by Five Frequently Methylated Genes Leads to Head and Neck Squamous Cell Carcinoma Pathogenesis

doi: 10.1158/0008-5472.can-09-3073

Figure Lengend Snippet: Figure 4. Functional relevance of the interactions between the methylated candidates and components of the TGF-β pathway. A, Western blot analysis of ARTS, caspase-3, TGF-β, and involucrin expression in the five overexpressing cell lines (tubulin serves as a loading control). B, cell cycle analysis of the five overexpressing cell lines revealed a significant decrease in mitosis in IRX1-, EBF3-, and SLC5A8-overexpressing cells. *, P < 0.05; **, P < 0.01.

Article Snippet: The antibodies used in the study were as follows: IL-6 (Abcam, ab6672), ARTS (Sigma-Aldrich, A4471), SLC5A8 (Santa Cruz, sc-34189), SEPT9 (aka MSF; Abgent, AP6215a), EBF3 (Aviva Systems Biology, ARP31837_T100), survivin (Santa Cruz, sc-17779), α-tubulin (Cell Signaling Technologies), TGF-β1 (Santa Cruz, sc-146), caspase-3 (Cell Signaling, 9662), actin (Santa Cruz, sc-8432), HIF-1α (Cell Signaling, 3716), and involucrin (Aviva Systems Biology, ARP48675_P050).

Techniques: Functional Assay, Methylation, Western Blot, Expressing, Control, Cell Cycle Assay