Journal: Cardiovascular research

Article Title: Functional tissue-engineered blood vessels from bone marrow progenitor cells.

doi: 10.1016/j.cardiores.2007.04.018

Figure Lengend Snippet: Fig. 1. BM-SMPC express biochemical markers of smooth muscle cells. (A) RT-PCR showed that BM-SMPC expressed smooth muscle cell markers as indicated. (B) Western blot for smooth muscle α-actin and calponin; beta-actin served as loading control. (C) Immunocytochemistry for smooth muscle α-actin and calponin. V-SMC from umbilical veins of near-term lambs were used as positive control. Representative results from three independent experiments are shown.

Article Snippet: Western blots were performed as described previously [33,34] using the following antibodies: mouse anti human smooth muscle α-actin (1:100 dilution; SeroTec) and mouse anti-human calponin (1:100 dilution; DakoCytomation) in TBS-Tween.

Techniques: Reverse Transcription Polymerase Chain Reaction, Western Blot, Control, Immunocytochemistry, Positive Control

Journal: Cardiovascular research

Article Title: Functional tissue-engineered blood vessels from bone marrow progenitor cells.

doi: 10.1016/j.cardiores.2007.04.018

Figure Lengend Snippet: Fig. 2. BM-TEV displayed similar morphologic and biochemical characteristics as TEVs from V-SMC. BM-SMPC or V-SMC were embedded in fibrin hydrogels and cultured around 4-mm mandrel for 2 weeks to form cylindrical tubes. (A) Hematoxylin and eosin (H&E) staining showed that BM-SMPC were distributed uniformly in fibrin hydrogels (bar=100 μm). (B) Immunostaining of BM-TEV and TEV from V-SMC for smooth muscle α-actin and calponin (bar=100 μm).

Article Snippet: Western blots were performed as described previously [33,34] using the following antibodies: mouse anti human smooth muscle α-actin (1:100 dilution; SeroTec) and mouse anti-human calponin (1:100 dilution; DakoCytomation) in TBS-Tween.

Techniques: Cell Culture, Staining, Immunostaining