Journal: BMC Microbiology

Article Title: Nontypeable Haemophilus influenzae exploits the interaction between protein-E and vitronectin for the adherence and invasion to bronchial epithelial cells

doi: 10.1186/s12866-015-0600-8

Figure Lengend Snippet: NTHi binding of immobilized vitronectin in the absence or presence of heparin. Human plasma vitronectin was bound to glass-bottomed dishes. NTHi was incubated on the plate-bound vitronectin for 30 minutes, and then the dishes were washed three times. In some experiments, plate-bound vitronectin was pretreated with heparin before incubation with NTHi. NTHi was stained with LIVE/DEAD®. Live NTHi is stained green. BSA was used as a negative control. Representative fluorescent micrographs show that both NTHi strains (ATCC 19418 ( a ) and HUSM 0481 ( b )) attached to plate-bound vitronectin and that this attachment is blocked by heparin. White bars represent 10 μm. Summaries of the numbers of attached NTHi (per field at 1,000× magnification) are shown in ( c ) for ATCC 19418 and ( d ) for HUSM 0481. Error bars represent SEM in three independent experiments that gave similar results. ** p < 0.01

Article Snippet: After washing with PBS, 5.0 μg/ml of monoclonal antibody to human vitronectin (Takara, Otsu, Japan) was added and incubated for 60 minutes.

Techniques: Binding Assay, Incubation, Staining, Negative Control

Journal: BMC Microbiology

Article Title: Nontypeable Haemophilus influenzae exploits the interaction between protein-E and vitronectin for the adherence and invasion to bronchial epithelial cells

doi: 10.1186/s12866-015-0600-8

Figure Lengend Snippet: Binding of NTHi to immobilized vitronectin in the presence of protein-E peptide. Human plasma vitronectin was bound to glass-bottomed dishes. NTHi (HUSM 0481) was incubated on the plate-bound vitronectin for 30 minutes, and then the dishes were washed three times. In some experiments, plate-bound vitronectin was pretreated with protein-E peptide (PE 84–108 ) before incubation with NTHi. BSA was used as a negative control. NTHi was stained with LIVE/DEAD®, and viable NTHi is stained green. a Representative fluorescent micrographs of NTHi incubated on plate-bound vitronectin that was untreated or pretreated with PE 84–108 . White bars represent 10 μm. b The number of attached NTHi per field at 1,000 × magnification that was pretreated with increasing concentrations of PE 84–108 . Error bars represent SEM in three independent experiments that gave similar results. ** p < 0.01

Article Snippet: After washing with PBS, 5.0 μg/ml of monoclonal antibody to human vitronectin (Takara, Otsu, Japan) was added and incubated for 60 minutes.

Techniques: Binding Assay, Incubation, Negative Control, Staining

Journal: BMC Microbiology

Article Title: Nontypeable Haemophilus influenzae exploits the interaction between protein-E and vitronectin for the adherence and invasion to bronchial epithelial cells

doi: 10.1186/s12866-015-0600-8

Figure Lengend Snippet: A schema of the proposed mechanism by which NTHi penetrates into bronchial epithelial cell via protein-E and vitronectin. Vitronectin has three heparin-binding domains (HBDs), which interact with NTHi. Of those HBDs, the C-terminal HBD-3 corresponds to a protein-E binding region and interacts with PE 84–108 . This interaction is blocked by heparin or PE 84–108 peptide. Vitronectin also possesses a cell receptor binding site characterized by an Arg-Gly-Asp (RGD) sequence, which interacts with integrins on the bronchial epithelial cell surface. This protein-E-vitronectin axis seems to play a role in the adherence and penetration of NTHi into bronchial epithelial cells

Article Snippet: After washing with PBS, 5.0 μg/ml of monoclonal antibody to human vitronectin (Takara, Otsu, Japan) was added and incubated for 60 minutes.

Techniques: Binding Assay, Sequencing

Journal: Veterinary Research

Article Title: Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) moonlights as an adhesin in Mycoplasma hyorhinis adhesion to epithelial cells as well as a plasminogen receptor mediating extracellular matrix degradation

doi: 10.1186/s13567-021-00952-8

Figure Lengend Snippet: Binding of the rGAPDH protein to different ECM components in ELISA experiment . Microtiters plate was coated with Matrigel, fibronectin, collagen or laminin solution. Various concentrations of rGAPDH or BSA were added and detected by anti-His-tag monoclonal antibody. For detecting the binding to vitronectin, microtiters plate was coated with rGAPDH or BSA. Various concentrations of vitronectin were added and detected by anti-vitronectin monoclonal antibody. * P < 0.05, ** P < 0.01, compared with the negative control (BSA).

Article Snippet: The bound vitronectin was detected by rabbit anti-vitronectin monoclonal antibody (1:2000; Boster, China), followed by goat anti-rabbit IgG (1:10 000).

Techniques: Binding Assay, Enzyme-linked Immunosorbent Assay, Negative Control

Journal: Journal of Biological Chemistry

Article Title: Plasminogen Activator Inhibitor-1 Regulates Tumor Growth and Angiogenesis

doi: 10.1074/jbc.m105980200

Figure Lengend Snippet: FIG. 3. Immuno-analysis of vessel architecture in M21 tumors. Tumor sections treated with PBS (A, D, G), partially inactivated PAI-1 (B, E, H), or fully active PAI-1 (C, F, I) were stained for the following: immunohistochemical staining for collagen IV (A–C), immunohisto- chemical staining for smooth muscle cell actin (D–F), immunofluores- cence analysis of vitronectin (green) (G–I), endothelial cells (red), and tumor cells (blue). The original magnification for all panels is 400.

Article Snippet: For immunofluorescence staining of tumor sections, slides were treated as above and then double stained with rabbit anti-murine vitronectin (Molecular Innovations) and rat anti-murine PECAM-1 (PharMingen) and detected with Flour 488 goat anti-rabbit IgG (Molecular Probes) and Texas Red rat anti-mouse IgG (Molecular Probes).

Techniques: Staining, Immunohistochemical staining

Journal: International Journal of Molecular Sciences

Article Title: Study on the Expression and Clinical Significances of Lewis y Antigen and Integrin αv, β3 in Epithelial Ovarian Tumors

doi: 10.3390/ijms12063409

Figure Lengend Snippet: Expression of integrin αv, β3 in different ovarian tissues.

Article Snippet: Rabbit anti-human integrin αv, β3 polyclonal antibody was purchased from Wuhan Boster Company, mouse anti-human Lewis y monoclonal antibody was purchased from Abcam Inc., biotinylated goat anti-mouse IgM, biotinylated goat anti-mouse IgG were purchased from Zhongshan Biotechnology Co., Ltd. Serum albumin (BSA) and DAB kit were purchased from Zhongshan Biotechnology Co., Ltd.

Techniques: Expressing

Journal: International Journal of Molecular Sciences

Article Title: Study on the Expression and Clinical Significances of Lewis y Antigen and Integrin αv, β3 in Epithelial Ovarian Tumors

doi: 10.3390/ijms12063409

Figure Lengend Snippet: Immunohistochemical staining in ovarian malignant tumor ( A1 – A3 ), borderline tumor ( B1 – B3 ), benign tumor ( C1 – C3 ) and normal ovarian tissue ( D1 – D3 ). Integrin αv ( A1 , B1 , C1 , D1 ); β3 ( A2 , B2 , C2 , D2 ) and Lewis y ( A3 , B3 , C3 , D3 ). (Original magnification ×200).

Article Snippet: Rabbit anti-human integrin αv, β3 polyclonal antibody was purchased from Wuhan Boster Company, mouse anti-human Lewis y monoclonal antibody was purchased from Abcam Inc., biotinylated goat anti-mouse IgM, biotinylated goat anti-mouse IgG were purchased from Zhongshan Biotechnology Co., Ltd. Serum albumin (BSA) and DAB kit were purchased from Zhongshan Biotechnology Co., Ltd.

Techniques: Immunohistochemical staining, Staining

Journal: International Journal of Molecular Sciences

Article Title: Study on the Expression and Clinical Significances of Lewis y Antigen and Integrin αv, β3 in Epithelial Ovarian Tumors

doi: 10.3390/ijms12063409

Figure Lengend Snippet: Relationship between integrin αv with the clinical and pathological parameters of malignant ovarian cancer.

Article Snippet: Rabbit anti-human integrin αv, β3 polyclonal antibody was purchased from Wuhan Boster Company, mouse anti-human Lewis y monoclonal antibody was purchased from Abcam Inc., biotinylated goat anti-mouse IgM, biotinylated goat anti-mouse IgG were purchased from Zhongshan Biotechnology Co., Ltd. Serum albumin (BSA) and DAB kit were purchased from Zhongshan Biotechnology Co., Ltd.

Techniques: Diffusion-based Assay

Journal: International Journal of Molecular Sciences

Article Title: Study on the Expression and Clinical Significances of Lewis y Antigen and Integrin αv, β3 in Epithelial Ovarian Tumors

doi: 10.3390/ijms12063409

Figure Lengend Snippet: Relationship between integrin β3 with the clinical and pathological parameters of malignant ovarian cancer.

Article Snippet: Rabbit anti-human integrin αv, β3 polyclonal antibody was purchased from Wuhan Boster Company, mouse anti-human Lewis y monoclonal antibody was purchased from Abcam Inc., biotinylated goat anti-mouse IgM, biotinylated goat anti-mouse IgG were purchased from Zhongshan Biotechnology Co., Ltd. Serum albumin (BSA) and DAB kit were purchased from Zhongshan Biotechnology Co., Ltd.

Techniques: Diffusion-based Assay

Journal: International Journal of Molecular Sciences

Article Title: Study on the Expression and Clinical Significances of Lewis y Antigen and Integrin αv, β3 in Epithelial Ovarian Tumors

doi: 10.3390/ijms12063409

Figure Lengend Snippet: Correlation between expression of Lewis y antigen and integrin αv in ovarian cancer.

Article Snippet: Rabbit anti-human integrin αv, β3 polyclonal antibody was purchased from Wuhan Boster Company, mouse anti-human Lewis y monoclonal antibody was purchased from Abcam Inc., biotinylated goat anti-mouse IgM, biotinylated goat anti-mouse IgG were purchased from Zhongshan Biotechnology Co., Ltd. Serum albumin (BSA) and DAB kit were purchased from Zhongshan Biotechnology Co., Ltd.

Techniques: Expressing

Journal: International Journal of Molecular Sciences

Article Title: Study on the Expression and Clinical Significances of Lewis y Antigen and Integrin αv, β3 in Epithelial Ovarian Tumors

doi: 10.3390/ijms12063409

Figure Lengend Snippet: Correlation between expression of Lewis y antigen and integrin β3 in ovarian cancer.

Article Snippet: Rabbit anti-human integrin αv, β3 polyclonal antibody was purchased from Wuhan Boster Company, mouse anti-human Lewis y monoclonal antibody was purchased from Abcam Inc., biotinylated goat anti-mouse IgM, biotinylated goat anti-mouse IgG were purchased from Zhongshan Biotechnology Co., Ltd. Serum albumin (BSA) and DAB kit were purchased from Zhongshan Biotechnology Co., Ltd.

Techniques: Expressing

Journal: International Journal of Molecular Sciences

Article Title: Study on the Expression and Clinical Significances of Lewis y Antigen and Integrin αv, β3 in Epithelial Ovarian Tumors

doi: 10.3390/ijms12063409

Figure Lengend Snippet: Integrin αv, β3 and Lewis y colocalize in ovarian malignant tumor. Using an immunofluorescent double-labeling method. Integrin αv and β3 ( A1 and B1 ); Lewis y ( A2 and B2 ); nucleus ( A3 and B3 ); Merged image ( A4 and B4 ). (Original magnification ×400).

Article Snippet: Rabbit anti-human integrin αv, β3 polyclonal antibody was purchased from Wuhan Boster Company, mouse anti-human Lewis y monoclonal antibody was purchased from Abcam Inc., biotinylated goat anti-mouse IgM, biotinylated goat anti-mouse IgG were purchased from Zhongshan Biotechnology Co., Ltd. Serum albumin (BSA) and DAB kit were purchased from Zhongshan Biotechnology Co., Ltd.

Techniques: Labeling