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Image Search Results
Journal: Frontiers in Immunology
Article Title: Impact of Enhancer of Zeste Homolog 2 on T Helper Cell-Mediated Allergic Rhinitis
doi: 10.3389/fimmu.2017.00790
Figure Lengend Snippet: House dust mite-induced enhancer of zeste homolog 2 (Ezh2)-mediated T-lymphocyte response was modified by p38 inhibitor. (A) Western blot analysis of Ezh2, p38, pp38, and Actin in Jurkat cells after adding p38 MAP kinase inhibitor, * p < 0.05, by the t -test. (B) Relative Ezh2 expression of Th1 cells to naïve CD4 + T cells with HDM stimulation with or without p38 MAP kinase inhibitor treatment on day 10. The mean fluorescence intensity (MFI) of Th1 cells divided by the MFI of naïve CD4 + T cells in the same tube [(MFI of Th1 cells/MFI of naïve CD4 + T cells) × 100%] ( n = 11). (C) The mean fold change of Th1 percentage in CD4 + T cells with HDM stimulation with or without p38 MAP kinase inhibitor treatment on day 10 compared to day 0 (the percentage on day 0/the percentage on day 10) ( n = 11). (D) Relative Ezh2 expression of Th2 cells to naïve CD4 + T cells with HDM stimulation with or without p38 MAP kinase inhibitor treatment on day 10. The MFI of Th1 cells divided by the MFI of naïve CD4 + T cells in the same tube [(MFI of Th2 cells/MFI of naïve CD4 + T cells) × 100%] ( n = 11). (E) The mean fold change of Th2 percentage in CD4 + T cells with HDM stimulation with or without p38 MAP kinase inhibitor treatment on day 10 compared to day 0 (the percentage on day 0/the percentage on day 10) ( n = 11 in each group).
Article Snippet: Antibodies used in this study described as follows: anti-p38 antibody (ABGENT, Catalog Number: AP3904a, San Diego, CA, USA);
Techniques: Modification, Western Blot, Expressing, Fluorescence