anti-munc13 Search Results


90
Synaptic Systems mouse monoclonal antimunc13-1
Mouse Monoclonal Antimunc13 1, supplied by Synaptic Systems, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/mouse monoclonal antimunc13-1/product/Synaptic Systems
Average 90 stars, based on 1 article reviews
mouse monoclonal antimunc13-1 - by Bioz Stars, 2026-04
90/100 stars
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90
Absolute Biotech Inc goat anti–munc13-4
CTLs from patients UPN396 and UPN324 show severely reduced ability to kill target cells. A, Percentage killing of P815 target cells by means of redirected lysis with anti-CD3 mediated by CTLs from a healthy donor ( HD ; triangles ), patient UPN324 (squares) , and patient UPN396 (circles) after 4 hours. Each data point was run in triplicates. The image is representative of 3 independent experiments. B, Immunoblots of Rab27a and <t>Munc13-4</t> in CTLs from a healthy donor (HD) and patients UPN324 and UPN396. Proteins from lysates were separated by means of SDS-PAGE, transferred onto nitrocellulose, and probed with protein-specific antibodies against Rab27a, Munc13-4, and actin. Images are representative of 3 independent experiments.
Goat Anti–Munc13 4, supplied by Absolute Biotech Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/goat anti–munc13-4/product/Absolute Biotech Inc
Average 90 stars, based on 1 article reviews
goat anti–munc13-4 - by Bioz Stars, 2026-04
90/100 stars
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UNC13D Munc13 4 Goat anti Human Polyclonal Internal Unconjugated Antibody 50 µg
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UNC13D Munc13 4 Goat anti Human Polyclonal C Terminus Unconjugated Antibody 50 µg
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Boster Bio UNC13D mouse monoclonal antibody, clone OTI1F4 (formerly 1F4). Catalog# M03088. Tested in WB. This antibody reacts with Human, Mouse, Rat.
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Image Search Results


CTLs from patients UPN396 and UPN324 show severely reduced ability to kill target cells. A, Percentage killing of P815 target cells by means of redirected lysis with anti-CD3 mediated by CTLs from a healthy donor ( HD ; triangles ), patient UPN324 (squares) , and patient UPN396 (circles) after 4 hours. Each data point was run in triplicates. The image is representative of 3 independent experiments. B, Immunoblots of Rab27a and Munc13-4 in CTLs from a healthy donor (HD) and patients UPN324 and UPN396. Proteins from lysates were separated by means of SDS-PAGE, transferred onto nitrocellulose, and probed with protein-specific antibodies against Rab27a, Munc13-4, and actin. Images are representative of 3 independent experiments.

Journal: The Journal of Allergy and Clinical Immunology

Article Title: Patients with Griscelli syndrome and normal pigmentation identify RAB27A mutations that selectively disrupt MUNC13-4 binding

doi: 10.1016/j.jaci.2014.08.039

Figure Lengend Snippet: CTLs from patients UPN396 and UPN324 show severely reduced ability to kill target cells. A, Percentage killing of P815 target cells by means of redirected lysis with anti-CD3 mediated by CTLs from a healthy donor ( HD ; triangles ), patient UPN324 (squares) , and patient UPN396 (circles) after 4 hours. Each data point was run in triplicates. The image is representative of 3 independent experiments. B, Immunoblots of Rab27a and Munc13-4 in CTLs from a healthy donor (HD) and patients UPN324 and UPN396. Proteins from lysates were separated by means of SDS-PAGE, transferred onto nitrocellulose, and probed with protein-specific antibodies against Rab27a, Munc13-4, and actin. Images are representative of 3 independent experiments.

Article Snippet: Lysates were precleared with 100 μL of Protein G Bead slurry (GE Healthcare, Fairfield, Conn) with rotating at 4°C for 30 minutes and then split into 3 samples: no antibody, rabbit anti-Rab27a (produced in rabbits immunized with human Rab27a-GST and screened against lysates from healthy donor and Rab27A-deficient human and mouse cell lines), and either goat anti–Munc13-4 (Everest Biotechnology, Upper Heyford, United Kingdom) or rabbit anti-Mlph (Abcam, Cambridge, United Kingdom).

Techniques: Lysis, Western Blot, SDS Page

Effect of mutations in Rab27a on binding to Mlph and Munc13-4. Immunoblots showing coimmunoprecipitations are shown. A, Munc13-4 with Rab27a ( lanes 1 and 2 ), Rab27a-S163R ( lanes 3 and 4 ), and Rab27a-R141lfs ( lanes 5 and 6 ). Images are representative of 3 independent experiments. B, Rab27a ( lanes 1 and 2 ), Rab27a-S163R ( lanes 3 and 4 ), and Rab27a-R141lfs ( lanes 5 and 6 ) with Mlph. Membranes were probed with specific antibodies against Rab27a (top) or Mlph (bottom) . C and D, As above but with Rab27A A76V and Y159C. Proteins were immunoprecipitated with anti-Munc13-4 or Mlph and probed for Rab27a, Munc13-4, and Mlph.

Journal: The Journal of Allergy and Clinical Immunology

Article Title: Patients with Griscelli syndrome and normal pigmentation identify RAB27A mutations that selectively disrupt MUNC13-4 binding

doi: 10.1016/j.jaci.2014.08.039

Figure Lengend Snippet: Effect of mutations in Rab27a on binding to Mlph and Munc13-4. Immunoblots showing coimmunoprecipitations are shown. A, Munc13-4 with Rab27a ( lanes 1 and 2 ), Rab27a-S163R ( lanes 3 and 4 ), and Rab27a-R141lfs ( lanes 5 and 6 ). Images are representative of 3 independent experiments. B, Rab27a ( lanes 1 and 2 ), Rab27a-S163R ( lanes 3 and 4 ), and Rab27a-R141lfs ( lanes 5 and 6 ) with Mlph. Membranes were probed with specific antibodies against Rab27a (top) or Mlph (bottom) . C and D, As above but with Rab27A A76V and Y159C. Proteins were immunoprecipitated with anti-Munc13-4 or Mlph and probed for Rab27a, Munc13-4, and Mlph.

Article Snippet: Lysates were precleared with 100 μL of Protein G Bead slurry (GE Healthcare, Fairfield, Conn) with rotating at 4°C for 30 minutes and then split into 3 samples: no antibody, rabbit anti-Rab27a (produced in rabbits immunized with human Rab27a-GST and screened against lysates from healthy donor and Rab27A-deficient human and mouse cell lines), and either goat anti–Munc13-4 (Everest Biotechnology, Upper Heyford, United Kingdom) or rabbit anti-Mlph (Abcam, Cambridge, United Kingdom).

Techniques: Binding Assay, Western Blot, Immunoprecipitation