Journal: Cells

Article Title: Enhanced Autophagic Flux, Suppressed Apoptosis and Reduced Macrophage Infiltration by Dasatinib in Kidneys of Obese Mice

doi: 10.3390/cells11040746

Figure Lengend Snippet: Sample size calculation.

Article Snippet: After blocking, an overnight membrane incubation with the primary antibodies—rabbit polyclonal antibody for LC3 and rabbit polyclonal antibody for SQSTM1/P62 as markers for autophagic activity, mouse monoclonal antibody for caspase-3 as an apoptosis marker and β-actin as a control protein (Biospes; YPA1652, ABclonal; A11250, Santa Cruz sc-7272 and Abcam ab227387, respectively)—was performed overnight at 4 °C (1:500, 1:1000, 1:100 and 1:5000 dilutions, respectively).

Techniques:

Journal: Cells

Article Title: Enhanced Autophagic Flux, Suppressed Apoptosis and Reduced Macrophage Infiltration by Dasatinib in Kidneys of Obese Mice

doi: 10.3390/cells11040746

Figure Lengend Snippet: ( A – D ) Protein analysis by Western blotting. ( A ) LC3 I and II (Molecular weight: 18 kDa and 16 kDa, respectively). ( B ) p62 (Molecular weight: 62 kDa). ( C ) Caspase-3: 32 kDa). ( D ) β-actin: 42 kDa). ( E – H ): LC3II, LC3II/LC3I ratio, P62 and caspase3 proteins relative quantitation /β-actin ratio) by Western blotting. Data are mentioned as mean ± SE; different letters = significant difference). LC3: Microtubule-associated proteins 1A/1B light chain 3B. M: Marker. MW: molecular weight.

Article Snippet: After blocking, an overnight membrane incubation with the primary antibodies—rabbit polyclonal antibody for LC3 and rabbit polyclonal antibody for SQSTM1/P62 as markers for autophagic activity, mouse monoclonal antibody for caspase-3 as an apoptosis marker and β-actin as a control protein (Biospes; YPA1652, ABclonal; A11250, Santa Cruz sc-7272 and Abcam ab227387, respectively)—was performed overnight at 4 °C (1:500, 1:1000, 1:100 and 1:5000 dilutions, respectively).

Techniques: Western Blot, Molecular Weight, Quantitation Assay, Marker

Journal: Cells

Article Title: Enhanced Autophagic Flux, Suppressed Apoptosis and Reduced Macrophage Infiltration by Dasatinib in Kidneys of Obese Mice

doi: 10.3390/cells11040746

Figure Lengend Snippet: ( A – D ): Immunohistochemical staining for LC3 (×400) in the kidney tissues of vehicle, Dasatinib control, Obesogenic diet and Obesogenic diet + Dasatinib groups ( A – D , respectively). Scale bar = 50 µm. ( E ) The mean percentage of LC3 immunopositive area. Results are presented as mean ± standard error. Different letters mean significant difference. Arrows: LC3 + ve cells. LC3 = Microtubule-associated protein light chain 3.

Article Snippet: After blocking, an overnight membrane incubation with the primary antibodies—rabbit polyclonal antibody for LC3 and rabbit polyclonal antibody for SQSTM1/P62 as markers for autophagic activity, mouse monoclonal antibody for caspase-3 as an apoptosis marker and β-actin as a control protein (Biospes; YPA1652, ABclonal; A11250, Santa Cruz sc-7272 and Abcam ab227387, respectively)—was performed overnight at 4 °C (1:500, 1:1000, 1:100 and 1:5000 dilutions, respectively).

Techniques: Immunohistochemical staining, Staining, Control

Journal: Evidence-based Complementary and Alternative Medicine : eCAM

Article Title: The Effects of Yuan-Zhi Decoction and Its Active Ingredients in Both In Vivo and In Vitro Models of Chronic Cerebral Hypoperfusion by Regulating the Levels of A β and Autophagy

doi: 10.1155/2020/6807879

Figure Lengend Snippet: The expression of Beclin-1 and LC3-II in the hippocampal neurons. Representative immunofluorescence images showing in situ expression of Beclin-1 (a) and LC3-II (b) in the differentially treated hippocampal neurons (magnification: ×100). Scale bar = 100 μ m. Quantification of Beclin-1 (c) and LC3-II (d) in the hippocampal neurons in each group (% of Control). Data are presented as mean ± SD. ## P < 0.01, vs control group; ∗ P < 0.05, ∗∗ P < 0.01, ∗∗∗ P < 0.001, vs model group.

Article Snippet: The cells were then incubated overnight with anti-Beclin-1 (1 : 200; ImmunoWay, USA) and anti-LC3-II (1 : 200; ImmunoWay, USA) antibodies at 4°C.

Techniques: Expressing, Immunofluorescence, In Situ

Journal: Evidence-based Complementary and Alternative Medicine : eCAM

Article Title: The Effects of Yuan-Zhi Decoction and Its Active Ingredients in Both In Vivo and In Vitro Models of Chronic Cerebral Hypoperfusion by Regulating the Levels of A β and Autophagy

doi: 10.1155/2020/6807879

Figure Lengend Snippet: The expression levels of Beclin-1, LC3-II, BACE1, PS-1, and ADAM17 in hippocampal neurons. Data are presented as mean ± SD. ## P < 0.01, vs control group; ∗∗ P < 0.01, ∗∗∗ P < 0.001, vs model group.

Article Snippet: The cells were then incubated overnight with anti-Beclin-1 (1 : 200; ImmunoWay, USA) and anti-LC3-II (1 : 200; ImmunoWay, USA) antibodies at 4°C.

Techniques: Expressing

Journal: Evidence-based Complementary and Alternative Medicine : eCAM

Article Title: The Effects of Yuan-Zhi Decoction and Its Active Ingredients in Both In Vivo and In Vitro Models of Chronic Cerebral Hypoperfusion by Regulating the Levels of A β and Autophagy

doi: 10.1155/2020/6807879

Figure Lengend Snippet: Correlation analysis between A β 1-40/A β 1-42 and the autophagy-related (Beclin-1 and LC3-II) and APP secretases-related proteins (BACE1, PS-1, and ADAM17) levels in hippocampal neurons. R 2 is the correlation coefficient.

Article Snippet: The cells were then incubated overnight with anti-Beclin-1 (1 : 200; ImmunoWay, USA) and anti-LC3-II (1 : 200; ImmunoWay, USA) antibodies at 4°C.

Techniques: