Journal: Frontiers in Oncology

Article Title: Combined Adjuvant of Poly I:C Improves Antitumor Effects of CAR-T Cells

doi: 10.3389/fonc.2019.00241

Figure Lengend Snippet: Administration of poly I: C decreases MDSC subunit cell count in tumor-bearing mice compared with saline-treated tumor-bearing mice. (A) representative FACS plots gated on CD11b+ Gr1+ cells in peripheral blood, spleen and tumor tissues of CT26-EGFRvIII tumor-bearing mice. (B) cumulative data from A, showing the relative ratio of MDSC. (C) representative FACS plots gated on CD11b+ Gr1+ cells in peripheral blood, spleen, and tumor tissues of E0771-EGFRvIII tumor-bearing mice. (D) cumulative data from B, showing the relative ratio of MDSC. * p < 0.05, *** p < 0.001.

Article Snippet: To deplete in vivo MDSC, mice were intraperitoneally injected with anti-Gr1 mAb (10 mg/kg, clone RB6-8C5, BioXell) 24 h before poly I:C initially and then three times per week for 2 weeks ( ).

Techniques: Cell Counting, Saline

Journal: Frontiers in Oncology

Article Title: Combined Adjuvant of Poly I:C Improves Antitumor Effects of CAR-T Cells

doi: 10.3389/fonc.2019.00241

Figure Lengend Snippet: Co-administration of CAR-T with poly I:C and MDSC depletion agent dramatically improved antitumor efficacy against CT26-EGFRvIII subcutaneous tumors. (A) tumor growth curve of tumor-bearing mice treated by poly I:C, anti-Gr1 Ab and CAR-T cells. (B) At the end of experiment, tumors were isolated from tumor-bearing mice and photographed. (C) At the end of experiment, tumors were isolated from tumor-bearing mice and weighed. Error bars show + SEM. n = 6/group. * p < 0.05, *** p < 0.001.

Article Snippet: To deplete in vivo MDSC, mice were intraperitoneally injected with anti-Gr1 mAb (10 mg/kg, clone RB6-8C5, BioXell) 24 h before poly I:C initially and then three times per week for 2 weeks ( ).

Techniques: Isolation

Journal: BMC Pharmacology & Toxicology

Article Title: Functional and molecular evidence for heteromeric association of P2Y 1 receptor with P2Y 2 and P2Y 4 receptors in mouse granulocytes

doi: 10.1186/s40360-016-0072-y

Figure Lengend Snippet: Presence of P2Y receptors in bone marrow granulocytes. a Granulocytes expressed mRNA for P2Y 1 , P2Y 2 , P2Y 4 and P2Y 12 receptors. Ladder 100-bp marker [M]; heart [H]; brain [B]; platelets [P]; granulocytes [G]. b To analyze the expression at the protein level of P2Y receptors in Gr-1 + Mac-1 + population (dot plot) flow cytometry was used. Solid black histogram: negative control (preincubated with the peptide antigen); open histogram: cells labeled with the respective antibodies

Article Snippet: FACs lysing solution and the monoclonal extracellular antibodies Phycoerythrin (PE)-conjugated rat anti-Mac-1 and Cy7/PE-conjugated rat anti-Gr-1 were purchased from PharMingen Becton Dickinson (CA, USA).

Techniques: Marker, Expressing, Flow Cytometry, Negative Control, Labeling