anti-epcam Search Results


95
Miltenyi Biotec apc conjugated mab to cd326 epcam
Gating strategy of lymphocytes, myeloid cells and tumor cells in peritoneal fluid. An example of gating method analyzed by Flow Jo software. Among the DAPI(+) peritoneal free cells, dead cells were excluded with FVS780. Tumor cells and leukocytes were defined as <t>CD45(-)CD326</t> (+) and CD45(+)CD326(-) cells, respectively, and tumor leukocyte ratio (TLR) was calculated as described in Material and Methods. Among the leukocyte populations, lymphocytes and myeloid cells were distinguished as FSC/SCC profile, and expression of antigens was further examined in each cell population.
Apc Conjugated Mab To Cd326 Epcam, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Boster Bio epcam
Gating strategy of lymphocytes, myeloid cells and tumor cells in peritoneal fluid. An example of gating method analyzed by Flow Jo software. Among the DAPI(+) peritoneal free cells, dead cells were excluded with FVS780. Tumor cells and leukocytes were defined as <t>CD45(-)CD326</t> (+) and CD45(+)CD326(-) cells, respectively, and tumor leukocyte ratio (TLR) was calculated as described in Material and Methods. Among the leukocyte populations, lymphocytes and myeloid cells were distinguished as FSC/SCC profile, and expression of antigens was further examined in each cell population.
Epcam, supplied by Boster Bio, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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96
Miltenyi Biotec anti epcam
Gating strategy of lymphocytes, myeloid cells and tumor cells in peritoneal fluid. An example of gating method analyzed by Flow Jo software. Among the DAPI(+) peritoneal free cells, dead cells were excluded with FVS780. Tumor cells and leukocytes were defined as <t>CD45(-)CD326</t> (+) and CD45(+)CD326(-) cells, respectively, and tumor leukocyte ratio (TLR) was calculated as described in Material and Methods. Among the leukocyte populations, lymphocytes and myeloid cells were distinguished as FSC/SCC profile, and expression of antigens was further examined in each cell population.
Anti Epcam, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Miltenyi Biotec cd326 epcam pe antibody
Gating strategy of lymphocytes, myeloid cells and tumor cells in peritoneal fluid. An example of gating method analyzed by Flow Jo software. Among the DAPI(+) peritoneal free cells, dead cells were excluded with FVS780. Tumor cells and leukocytes were defined as <t>CD45(-)CD326</t> (+) and CD45(+)CD326(-) cells, respectively, and tumor leukocyte ratio (TLR) was calculated as described in Material and Methods. Among the leukocyte populations, lymphocytes and myeloid cells were distinguished as FSC/SCC profile, and expression of antigens was further examined in each cell population.
Cd326 Epcam Pe Antibody, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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fluidigm 89y cd45 hi30 fluidigm 3089003b 141pr epcam
Gating strategy of lymphocytes, myeloid cells and tumor cells in peritoneal fluid. An example of gating method analyzed by Flow Jo software. Among the DAPI(+) peritoneal free cells, dead cells were excluded with FVS780. Tumor cells and leukocytes were defined as <t>CD45(-)CD326</t> (+) and CD45(+)CD326(-) cells, respectively, and tumor leukocyte ratio (TLR) was calculated as described in Material and Methods. Among the leukocyte populations, lymphocytes and myeloid cells were distinguished as FSC/SCC profile, and expression of antigens was further examined in each cell population.
89y Cd45 Hi30 Fluidigm 3089003b 141pr Epcam, supplied by fluidigm, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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94
Miltenyi Biotec cd326 epcam antibody
3D light sheet and 2D multi-cyclic imaging data comparison (Human OvCa) (A) Imaris 3D surface rendering of autofluorescence (cyan) and <t>CD326</t> positive cells (red). (B) Imaris 3D surface rendering of autofluorescence (cyan) with target plane in yellow. (C) Light sheet guided target plane selection representing CD326 positive cell (purple), CD45 positive cells (red), and CD3 positive cells (green). (D) DAPI overview image of selected tissue slice for 2D MACSima™ imaging. (E) Magnified merged six color multiparameter MICS image with CD45 (green), CD326 (cyan), FOLR1 (purple), Collagen III (red), Collagen IV (red), and CD31 (yellow). (F–L) Single staining MICS images (white) of DAPI (F), CD45 (G), CD326 (H), FOLR1 (I), Collagen III (J), Collagen IV (K), and CD31 (L) (gray) (see “Antibodies”). Scale bars: (A–F) 1 mm; (E) 250 μm; (F–L) 500 μm.
Cd326 Epcam Antibody, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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96
Miltenyi Biotec allophycocyanin apc conjugated mouse anti human epcam
3D light sheet and 2D multi-cyclic imaging data comparison (Human OvCa) (A) Imaris 3D surface rendering of autofluorescence (cyan) and <t>CD326</t> positive cells (red). (B) Imaris 3D surface rendering of autofluorescence (cyan) with target plane in yellow. (C) Light sheet guided target plane selection representing CD326 positive cell (purple), CD45 positive cells (red), and CD3 positive cells (green). (D) DAPI overview image of selected tissue slice for 2D MACSima™ imaging. (E) Magnified merged six color multiparameter MICS image with CD45 (green), CD326 (cyan), FOLR1 (purple), Collagen III (red), Collagen IV (red), and CD31 (yellow). (F–L) Single staining MICS images (white) of DAPI (F), CD45 (G), CD326 (H), FOLR1 (I), Collagen III (J), Collagen IV (K), and CD31 (L) (gray) (see “Antibodies”). Scale bars: (A–F) 1 mm; (E) 250 μm; (F–L) 500 μm.
Allophycocyanin Apc Conjugated Mouse Anti Human Epcam, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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95
Miltenyi Biotec cd326
3D light sheet and 2D multi-cyclic imaging data comparison (Human OvCa) (A) Imaris 3D surface rendering of autofluorescence (cyan) and <t>CD326</t> positive cells (red). (B) Imaris 3D surface rendering of autofluorescence (cyan) with target plane in yellow. (C) Light sheet guided target plane selection representing CD326 positive cell (purple), CD45 positive cells (red), and CD3 positive cells (green). (D) DAPI overview image of selected tissue slice for 2D MACSima™ imaging. (E) Magnified merged six color multiparameter MICS image with CD45 (green), CD326 (cyan), FOLR1 (purple), Collagen III (red), Collagen IV (red), and CD31 (yellow). (F–L) Single staining MICS images (white) of DAPI (F), CD45 (G), CD326 (H), FOLR1 (I), Collagen III (J), Collagen IV (K), and CD31 (L) (gray) (see “Antibodies”). Scale bars: (A–F) 1 mm; (E) 250 μm; (F–L) 500 μm.
Cd326, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Miltenyi Biotec fitc hea 125 miltenyi
3D light sheet and 2D multi-cyclic imaging data comparison (Human OvCa) (A) Imaris 3D surface rendering of autofluorescence (cyan) and <t>CD326</t> positive cells (red). (B) Imaris 3D surface rendering of autofluorescence (cyan) with target plane in yellow. (C) Light sheet guided target plane selection representing CD326 positive cell (purple), CD45 positive cells (red), and CD3 positive cells (green). (D) DAPI overview image of selected tissue slice for 2D MACSima™ imaging. (E) Magnified merged six color multiparameter MICS image with CD45 (green), CD326 (cyan), FOLR1 (purple), Collagen III (red), Collagen IV (red), and CD31 (yellow). (F–L) Single staining MICS images (white) of DAPI (F), CD45 (G), CD326 (H), FOLR1 (I), Collagen III (J), Collagen IV (K), and CD31 (L) (gray) (see “Antibodies”). Scale bars: (A–F) 1 mm; (E) 250 μm; (F–L) 500 μm.
Fitc Hea 125 Miltenyi, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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96
Miltenyi Biotec anti human cd326 epcam antibody
3D light sheet and 2D multi-cyclic imaging data comparison (Human OvCa) (A) Imaris 3D surface rendering of autofluorescence (cyan) and <t>CD326</t> positive cells (red). (B) Imaris 3D surface rendering of autofluorescence (cyan) with target plane in yellow. (C) Light sheet guided target plane selection representing CD326 positive cell (purple), CD45 positive cells (red), and CD3 positive cells (green). (D) DAPI overview image of selected tissue slice for 2D MACSima™ imaging. (E) Magnified merged six color multiparameter MICS image with CD45 (green), CD326 (cyan), FOLR1 (purple), Collagen III (red), Collagen IV (red), and CD31 (yellow). (F–L) Single staining MICS images (white) of DAPI (F), CD45 (G), CD326 (H), FOLR1 (I), Collagen III (J), Collagen IV (K), and CD31 (L) (gray) (see “Antibodies”). Scale bars: (A–F) 1 mm; (E) 250 μm; (F–L) 500 μm.
Anti Human Cd326 Epcam Antibody, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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fluidigm anti human cd326 epcam 9c4 antibody
3D light sheet and 2D multi-cyclic imaging data comparison (Human OvCa) (A) Imaris 3D surface rendering of autofluorescence (cyan) and <t>CD326</t> positive cells (red). (B) Imaris 3D surface rendering of autofluorescence (cyan) with target plane in yellow. (C) Light sheet guided target plane selection representing CD326 positive cell (purple), CD45 positive cells (red), and CD3 positive cells (green). (D) DAPI overview image of selected tissue slice for 2D MACSima™ imaging. (E) Magnified merged six color multiparameter MICS image with CD45 (green), CD326 (cyan), FOLR1 (purple), Collagen III (red), Collagen IV (red), and CD31 (yellow). (F–L) Single staining MICS images (white) of DAPI (F), CD45 (G), CD326 (H), FOLR1 (I), Collagen III (J), Collagen IV (K), and CD31 (L) (gray) (see “Antibodies”). Scale bars: (A–F) 1 mm; (E) 250 μm; (F–L) 500 μm.
Anti Human Cd326 Epcam 9c4 Antibody, supplied by fluidigm, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Miltenyi Biotec cd326 epcam miltenyi biotec
3D light sheet and 2D multi-cyclic imaging data comparison (Human OvCa) (A) Imaris 3D surface rendering of autofluorescence (cyan) and <t>CD326</t> positive cells (red). (B) Imaris 3D surface rendering of autofluorescence (cyan) with target plane in yellow. (C) Light sheet guided target plane selection representing CD326 positive cell (purple), CD45 positive cells (red), and CD3 positive cells (green). (D) DAPI overview image of selected tissue slice for 2D MACSima™ imaging. (E) Magnified merged six color multiparameter MICS image with CD45 (green), CD326 (cyan), FOLR1 (purple), Collagen III (red), Collagen IV (red), and CD31 (yellow). (F–L) Single staining MICS images (white) of DAPI (F), CD45 (G), CD326 (H), FOLR1 (I), Collagen III (J), Collagen IV (K), and CD31 (L) (gray) (see “Antibodies”). Scale bars: (A–F) 1 mm; (E) 250 μm; (F–L) 500 μm.
Cd326 Epcam Miltenyi Biotec, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Gating strategy of lymphocytes, myeloid cells and tumor cells in peritoneal fluid. An example of gating method analyzed by Flow Jo software. Among the DAPI(+) peritoneal free cells, dead cells were excluded with FVS780. Tumor cells and leukocytes were defined as CD45(-)CD326 (+) and CD45(+)CD326(-) cells, respectively, and tumor leukocyte ratio (TLR) was calculated as described in Material and Methods. Among the leukocyte populations, lymphocytes and myeloid cells were distinguished as FSC/SCC profile, and expression of antigens was further examined in each cell population.

Journal: Frontiers in Immunology

Article Title: Altered intraperitoneal immune microenvironment in patients with peritoneal metastases from gastric cancer

doi: 10.3389/fimmu.2022.969468

Figure Lengend Snippet: Gating strategy of lymphocytes, myeloid cells and tumor cells in peritoneal fluid. An example of gating method analyzed by Flow Jo software. Among the DAPI(+) peritoneal free cells, dead cells were excluded with FVS780. Tumor cells and leukocytes were defined as CD45(-)CD326 (+) and CD45(+)CD326(-) cells, respectively, and tumor leukocyte ratio (TLR) was calculated as described in Material and Methods. Among the leukocyte populations, lymphocytes and myeloid cells were distinguished as FSC/SCC profile, and expression of antigens was further examined in each cell population.

Article Snippet: APC-conjugated mAb to CD326 (EpCAM) was purchased from Miltenyi Biotec (Auburn, CA).

Techniques: Software, Expressing

3D light sheet and 2D multi-cyclic imaging data comparison (Human OvCa) (A) Imaris 3D surface rendering of autofluorescence (cyan) and CD326 positive cells (red). (B) Imaris 3D surface rendering of autofluorescence (cyan) with target plane in yellow. (C) Light sheet guided target plane selection representing CD326 positive cell (purple), CD45 positive cells (red), and CD3 positive cells (green). (D) DAPI overview image of selected tissue slice for 2D MACSima™ imaging. (E) Magnified merged six color multiparameter MICS image with CD45 (green), CD326 (cyan), FOLR1 (purple), Collagen III (red), Collagen IV (red), and CD31 (yellow). (F–L) Single staining MICS images (white) of DAPI (F), CD45 (G), CD326 (H), FOLR1 (I), Collagen III (J), Collagen IV (K), and CD31 (L) (gray) (see “Antibodies”). Scale bars: (A–F) 1 mm; (E) 250 μm; (F–L) 500 μm.

Journal: STAR Protocols

Article Title: Protocol for 3D-guided sectioning and deep cell phenotyping via light sheet imaging and 2D spatial multiplexing

doi: 10.1016/j.xpro.2025.104296

Figure Lengend Snippet: 3D light sheet and 2D multi-cyclic imaging data comparison (Human OvCa) (A) Imaris 3D surface rendering of autofluorescence (cyan) and CD326 positive cells (red). (B) Imaris 3D surface rendering of autofluorescence (cyan) with target plane in yellow. (C) Light sheet guided target plane selection representing CD326 positive cell (purple), CD45 positive cells (red), and CD3 positive cells (green). (D) DAPI overview image of selected tissue slice for 2D MACSima™ imaging. (E) Magnified merged six color multiparameter MICS image with CD45 (green), CD326 (cyan), FOLR1 (purple), Collagen III (red), Collagen IV (red), and CD31 (yellow). (F–L) Single staining MICS images (white) of DAPI (F), CD45 (G), CD326 (H), FOLR1 (I), Collagen III (J), Collagen IV (K), and CD31 (L) (gray) (see “Antibodies”). Scale bars: (A–F) 1 mm; (E) 250 μm; (F–L) 500 μm.

Article Snippet: CD326 (EpCam) antibody, anti-human, Vio 780, REAfinity , Miltenyi Biotec B.V. & Co. KG , Cat# 130-136-631 RRID: AB_2904949.

Techniques: Imaging, Comparison, Selection, Staining