Journal: Journal of Immunology Research

Article Title: Unveiling the Interplay Between Dendritic Cells and Natural Killer Cells as Key Players in Leishmania Infection

doi: 10.1155/jimr/3176927

Figure Lengend Snippet: Morphology and immunophenotype of NK cells. Negative magnetic selection was used to isolate NK cells from dog PBMCs. NK cells were labeled with CD3, CD16, CD21, CD94, and MHCII mononuclear antibodies directed conjugated and analyzed using multiparametric flow cytometry (A). Representative single-cell dot plots and histograms of labeled cell populations are shown. A digital image of sorted NK cells observed by inverted light microscopy is included (B).

Article Snippet: Cells washed twice with cold 1 × PBS (300 × g, 10 min, 4°C) were incubated (30 min, 4°C) in PBS 2% hiFBS with the following monoclonal antibodies directed conjugated: FICT-conjugated mouse antidog CD3 (clone CA17.2A12, Bio-Rad), Alexa Fluor 647-conjugated mouse anticanine CD21 (clone CA2.1D6, Bio-Rad), APC-conjugated mouse antidog CD94 monoclonal antibody (clone HP-3D9, eBioscience), FICT-conjugated Human CD16 monoclonal antibody (clone eBioCB16, eBioscience), and FICT-conjugated rat antidog DLA region MHC class II monomorphic (clone YKIX334.2, Bio-Rad) (Table ).

Techniques: Selection, Labeling, Flow Cytometry, Light Microscopy