Journal: Stem Cell Research & Therapy

Article Title: Connective tissue growth factor promotes cementogenesis and cementum repair via Cx43/β-catenin axis

doi: 10.1186/s13287-022-03149-8

Figure Lengend Snippet: CTGF promoted cementogenesis of hPDLSCs in vitro. A ARS staining showed a greater increase in mineralized nodules in hPDLSCs treated with the ascending-concentration of CTGF relative to the control group on the 14th day. B ALP staining on day 7 demonstrated the enhanced ALP activity in hPDLSCs after CTGF treatment. C Representative WB results of the mineralization-related proteins showed increasing expression of Osx, Runx2, CAP and Col1 in hPDLSCs when stimulated with the concentration-ascending CTGF on the 14th day. D – G Representative charts indicating the quantification of Osx, Runx2, CAP and Col1 in Fig. C. n = 3. Data are obtained from three independent images. * p < 0.05. H – L qPCR data showed that the expression of mineralization-related factors (Runx2, Osx, CAP, CEMP, ALP) enhanced in hPDLSCs treated with the concentration-ascending CTGF on the 14th day. Data are obtained from three independent results. * p < 0.05

Article Snippet: After incubation with 5% BSA, the sections were incubated overnight with anti-Col1 (1:200, 501,352, Zen-Bio, China), anti-Osterix (1:200, Huabio, ER1914-47, China), anti-Runx2 (1:200, 860,139, Zen-Bio, China), anti-CAP (1:200, sc-53947, Santa, America), anti-Cx43 (1:200, 340,279, Zen-Bio, China), and anti-active β-catenin (1:200, 19,807, Cell Signaling Technology, America) antibodies, followed by incubation with the corresponding IgG secondary antibodies.

Techniques: In Vitro, Staining, Concentration Assay, Activity Assay, Expressing

Journal: Stem Cell Research & Therapy

Article Title: Connective tissue growth factor promotes cementogenesis and cementum repair via Cx43/β-catenin axis

doi: 10.1186/s13287-022-03149-8

Figure Lengend Snippet: Cx43 expression was consistent with the expression of mineralization-related proteins induced by CTGF in vivo. A , B Representative IHC staining illustrated the protein expression of Osx and Runx2 with or without the treatment of CTGF on 0th day, 14th and 28th day. Images derived from three independent data. C , D Representative IHC staining illustrated the protein expression of CAP and Col1 with or without the treatment of CTGF on 0th day, 14th and 28th day. Images derived from three independent data. E , F The means of IOD of Osx and Runx2 in Fig. A, B. * p < 0.05. G , H The means of IOD of CAP and Cx43in Fig. A, B. * p < 0.05. I IHC results represented that the protein abundance of Cx43 with or without the treatment of CTGF on 0th day, 14th and 28th day. Images derived from three independent data. J The means of IOD of CAP and Cx43in Fig. A, B. * p < 0.05. K IF results represented that the abundance of Cx43 in CTGF-treated periodontal tissues. Cx43, red; nucleus, blue

Article Snippet: After incubation with 5% BSA, the sections were incubated overnight with anti-Col1 (1:200, 501,352, Zen-Bio, China), anti-Osterix (1:200, Huabio, ER1914-47, China), anti-Runx2 (1:200, 860,139, Zen-Bio, China), anti-CAP (1:200, sc-53947, Santa, America), anti-Cx43 (1:200, 340,279, Zen-Bio, China), and anti-active β-catenin (1:200, 19,807, Cell Signaling Technology, America) antibodies, followed by incubation with the corresponding IgG secondary antibodies.

Techniques: Expressing, In Vivo, Immunohistochemistry, Derivative Assay

Journal: Stem Cell Research & Therapy

Article Title: Connective tissue growth factor promotes cementogenesis and cementum repair via Cx43/β-catenin axis

doi: 10.1186/s13287-022-03149-8

Figure Lengend Snippet: Positive cementogenesis induced by CTGF was reversed by ablation of Cx43. A Western blotting demonstrated the abundance of Cx43 in hPDLSCs treated with Si-Cx43. B Bar chart showed the levels of Cx43 in Fig. A ratio to the untreated groups. Data are obtained from three independent images. n = 3, * p < 0.05. C Representative images of ARS indicated the attenuated ability of mineralization induced by Si-Cx43 in hPDLSCs compared with the untreated groups. D Representative images of ARS staining showed the CTGF-mediated enhancement in mineralized nodules was reversed by the addition of Si-Cx43. E Representative images of ALP staining showed the CTGF-mediated enhancement in ALP activity was reversed by Si-Cx43. F Representative images of the data of mineralization-associated proteins (Runx2, Osx, CAP, Col1) and Cx43 obtained by western blotting showed that increased mineralization capacity of CTGF expression was reverted by Si-Cx43. G – K Bar chart showed the levels of Runx2, Osx, CAP, Col1 and Cx43 in Fig. F ratio to the untreated control. Data are obtained from three independent images. n = 3, * p < 0.05. L–P mRNA expression of Runx2, Osx, ALP, CAP and CEMP, as detected by PCR ( n = 3) as detected by PCR

Article Snippet: After incubation with 5% BSA, the sections were incubated overnight with anti-Col1 (1:200, 501,352, Zen-Bio, China), anti-Osterix (1:200, Huabio, ER1914-47, China), anti-Runx2 (1:200, 860,139, Zen-Bio, China), anti-CAP (1:200, sc-53947, Santa, America), anti-Cx43 (1:200, 340,279, Zen-Bio, China), and anti-active β-catenin (1:200, 19,807, Cell Signaling Technology, America) antibodies, followed by incubation with the corresponding IgG secondary antibodies.

Techniques: Western Blot, Staining, Activity Assay, Expressing

Journal: Stem Cell Research & Therapy

Article Title: Connective tissue growth factor promotes cementogenesis and cementum repair via Cx43/β-catenin axis

doi: 10.1186/s13287-022-03149-8

Figure Lengend Snippet: Negative cementogenesis induced by Si-CTGF in hPDLSCs was motivated by the Cx43 agonist. A Western blotting demonstrated the abundance of CTGF in hPDLSCs treated with Si-CTGF. B Bar chart showed the levels of CTGF in A ratio to the untreated groups. Data are obtained from three independent images. n = 3, * p < 0.05. C Western blotting demonstrated the abundance of Cx43 in hPDLSCs treated with Cx43 agonist ATRA. D Bar chart showed the levels of Cx43 in C ratio to the untreated groups. Data are obtained from three independent images. n = 3, * p < 0.05. E Representative images of ARS indicated the attenuated ability of mineralization induced by Si-CTGF in hPDLSCs was regained by Cx43 agonist ATRA when compared with the untreated groups. F Representative images of ALP staining showed the attenuated ALP activity stimulated by Si-CTGF in hPDLSCs was regained by Cx43 agonist ATRA when in comparison to the untreated groups. G Representative image of the data of mineralization-associated proteins (Runx2, Osx, CAP, Col1) and Cx43 obtained by western blotting showed that the decreased mineralization capacity of CTGF expression was reverted by Cx43 agonist ATRA. H , I Bar chart showed the protein levels of Runx2, Osx, CAP, Col1 and Cx43 in G ratio to the untreated groups. Data are obtained from 3 independent images. n = 3, * p < 0.05. J mRNA expression of Osx, Runx2, ALP, CAP and CEMP, as detected by PCR ( n = 3) as detected by PCR

Article Snippet: After incubation with 5% BSA, the sections were incubated overnight with anti-Col1 (1:200, 501,352, Zen-Bio, China), anti-Osterix (1:200, Huabio, ER1914-47, China), anti-Runx2 (1:200, 860,139, Zen-Bio, China), anti-CAP (1:200, sc-53947, Santa, America), anti-Cx43 (1:200, 340,279, Zen-Bio, China), and anti-active β-catenin (1:200, 19,807, Cell Signaling Technology, America) antibodies, followed by incubation with the corresponding IgG secondary antibodies.

Techniques: Western Blot, Staining, Activity Assay, Expressing