Journal: Journal of Clinical Laboratory Analysis

Article Title: High‐dimensional single‐cell proteomics analysis reveals the landscape of immune cells and stem‐like cells in renal tumors

doi: 10.1002/jcla.23155

Figure Lengend Snippet: Purified antibodies about the immune cell‐centric panel

Article Snippet: CD86 , 156Gd , IT2.2 , Fluidigm , 3156008B.

Techniques: Purification

Journal: Frontiers in Oncology

Article Title: Tumor-associated macrophage expression in colorectal adenomas and carcinomas: relationship to Helicobacter pylori infection

doi: 10.3389/fonc.2025.1649619

Figure Lengend Snippet: Immunohistochemical detection of CD163 + and CD86 + TAMs in colorectal tissues (×200). (A) CD163 staining in various tissues. (B) CD86 staining. (C) IOD for CD163. (D) IOD for CD86. Data expressed as M (Q1, Q3) (n = 109). * P < 0.05, ** P < 0.01, *** P < 0.001 (DB-adjusted). Groups: 1, Normal; 2, CAS; 3, SSA; 4, CRC. M: Median; Q 1 : 1st Quartile; Q 3 : 3rd Quartile.

Article Snippet: The following reagents and equipment were used in this study: mouse monoclonal anti-CD163 antibody (10D6, ma5-11458, Invitrogen, Waltham, MA02451, USA); rabbit monoclonal anti-CD86 antibody (EP1158-37, ab269587, Abcam, Cambridge, UK); rabbit Polyclonal a nti-CD68 antibody ( GB113150 , Servicebio, Wuhan, China); rabbit Polyclonal anti-CD163 antibody ( GB113152 , Servicebio, Wuhan, China); rabbit Polyclonal anti-CD86 antibody ( GB115630 , Servicebio, Wuhan, China); secondary antibodies and DAB chromogenic kits (Biomiky, Biosharp, and Servicebio, respectively).

Techniques: Immunohistochemical staining, Staining

Journal: Frontiers in Oncology

Article Title: Tumor-associated macrophage expression in colorectal adenomas and carcinomas: relationship to Helicobacter pylori infection

doi: 10.3389/fonc.2025.1649619

Figure Lengend Snippet: Correlation of CD163 + /CD86 + TAMs levels with CRC development. (A) Differential expression between H.pylori -infected and uninfected groups. (B) Correlation between CD163 + and CD86 + expression. (C, D) Positive correlation of both markers with malignancy grade. Groups: 1, Normal; 2, CAS; 3, SSA; 4, CRC. *** P < 0.001 (MWU test).

Article Snippet: The following reagents and equipment were used in this study: mouse monoclonal anti-CD163 antibody (10D6, ma5-11458, Invitrogen, Waltham, MA02451, USA); rabbit monoclonal anti-CD86 antibody (EP1158-37, ab269587, Abcam, Cambridge, UK); rabbit Polyclonal a nti-CD68 antibody ( GB113150 , Servicebio, Wuhan, China); rabbit Polyclonal anti-CD163 antibody ( GB113152 , Servicebio, Wuhan, China); rabbit Polyclonal anti-CD86 antibody ( GB115630 , Servicebio, Wuhan, China); secondary antibodies and DAB chromogenic kits (Biomiky, Biosharp, and Servicebio, respectively).

Techniques: Quantitative Proteomics, Infection, Expressing

Journal: Frontiers in Oncology

Article Title: Tumor-associated macrophage expression in colorectal adenomas and carcinomas: relationship to Helicobacter pylori infection

doi: 10.3389/fonc.2025.1649619

Figure Lengend Snippet: Multiplex immunofluorescence (200×) : CD68 + (red), CD163 + (green), CD86 + (green), DAPI (nuclei, blue), Merge (multichannel overlay), Scale bar: 50 μm. (A) CD68 + CD163 + dual-positive cells (IF). (B) CD68 + CD86 + dual-positive cells (IF).

Article Snippet: The following reagents and equipment were used in this study: mouse monoclonal anti-CD163 antibody (10D6, ma5-11458, Invitrogen, Waltham, MA02451, USA); rabbit monoclonal anti-CD86 antibody (EP1158-37, ab269587, Abcam, Cambridge, UK); rabbit Polyclonal a nti-CD68 antibody ( GB113150 , Servicebio, Wuhan, China); rabbit Polyclonal anti-CD163 antibody ( GB113152 , Servicebio, Wuhan, China); rabbit Polyclonal anti-CD86 antibody ( GB115630 , Servicebio, Wuhan, China); secondary antibodies and DAB chromogenic kits (Biomiky, Biosharp, and Servicebio, respectively).

Techniques: Multiplex Assay, Immunofluorescence

Journal: Frontiers in Oncology

Article Title: Tumor-associated macrophage expression in colorectal adenomas and carcinomas: relationship to Helicobacter pylori infection

doi: 10.3389/fonc.2025.1649619

Figure Lengend Snippet: Changes in cell density and proportion of CD68 + CD163 +/ CD68 + CD86 + dual-positive TAMs across groups. Groups: 1, Normal; 2, CRA; 3, CRC. *** P < 0.001 (Tukey HSD).

Article Snippet: The following reagents and equipment were used in this study: mouse monoclonal anti-CD163 antibody (10D6, ma5-11458, Invitrogen, Waltham, MA02451, USA); rabbit monoclonal anti-CD86 antibody (EP1158-37, ab269587, Abcam, Cambridge, UK); rabbit Polyclonal a nti-CD68 antibody ( GB113150 , Servicebio, Wuhan, China); rabbit Polyclonal anti-CD163 antibody ( GB113152 , Servicebio, Wuhan, China); rabbit Polyclonal anti-CD86 antibody ( GB115630 , Servicebio, Wuhan, China); secondary antibodies and DAB chromogenic kits (Biomiky, Biosharp, and Servicebio, respectively).

Techniques:

Journal: Molecular Biomedicine

Article Title: Deleting fibroblast growth factor 2 in macrophages aggravates septic acute lung injury by increasing M1 polarization and inflammatory cytokine secretion

doi: 10.1186/s43556-024-00203-0

Figure Lengend Snippet: Effect of FGF2 deficiency on BMDM apoptosis and polarization. a – c FGF2 deletion increased BMDM apoptosis. a Apoptosis in BMDM deprived of FBS for 24 h was assessed by flow cytometry ( n = 4). b - c Percentage of PI + Annexin V + and PI- Annexin V + BMDM after starvation. d - k FGF2 deletion in BMDM promoted M1 polarization. d - g Flow cytometric analysis of macrophage markers in BMDM treated with LPS or IL4, including CD86, iNOS, CD206, and Arg1 ( n = 3). h - k The levels of CD86, iNOS, CD206 and Arg1 in BMDM after treatment with LPS or IL4. N represents no treatment; * p < 0.05, vs. WT; Ψ p < 0.05, vs. N + WT; Ω p < 0.05, vs. N + FGF2 KO

Article Snippet: The tissue sections were incubated overnight at 4°C with primary antibodies, including the rabbit polyclonal antibody CD206 (1:200, Cat No. A02285-2, Boster, Wuhan, China), rabbit monoclonal antibody CD86 (1:100, Cat No. BM4121, Boster, Wuhan, China), rabbit polyclonal antibody F4/80 (1:100, Cat No. 29414-1-AP, Proteintech, Wuhan, China), anti-mouse NLRP3 (1:200, Cat No.68102-1-Ig, Proteintech, Wuhan, China), Caspase-1 p20 Rabbit pAb (1:400, Cat No.bs-10743R, Bioss, Beijing, China) and ASC/TMS1 Rabbit PolyAb (1:200, Cat No.10500-1-AP, Proteintech, Wuhan, China).

Techniques: Flow Cytometry

Journal: Molecular Biomedicine

Article Title: Deleting fibroblast growth factor 2 in macrophages aggravates septic acute lung injury by increasing M1 polarization and inflammatory cytokine secretion

doi: 10.1186/s43556-024-00203-0

Figure Lengend Snippet: Mice reconstituted with FGF2 KO macrophages and subjected to CLP demonstrate increased M1 polarization in lung tissue. a - f The presence and levels of CD206, CD86, and F4/80 markers on macrophages within lung tissue were identified and quantitatively assessed using immunofluorescence staining. Bar is 20 μm. * p < 0.05, vs. WT; Δ p < 0.05 vs. WT + LPS; # p < 0.05 vs. FGF2 KO

Article Snippet: The tissue sections were incubated overnight at 4°C with primary antibodies, including the rabbit polyclonal antibody CD206 (1:200, Cat No. A02285-2, Boster, Wuhan, China), rabbit monoclonal antibody CD86 (1:100, Cat No. BM4121, Boster, Wuhan, China), rabbit polyclonal antibody F4/80 (1:100, Cat No. 29414-1-AP, Proteintech, Wuhan, China), anti-mouse NLRP3 (1:200, Cat No.68102-1-Ig, Proteintech, Wuhan, China), Caspase-1 p20 Rabbit pAb (1:400, Cat No.bs-10743R, Bioss, Beijing, China) and ASC/TMS1 Rabbit PolyAb (1:200, Cat No.10500-1-AP, Proteintech, Wuhan, China).

Techniques: Immunofluorescence, Staining

Journal: Biological Procedures Online

Article Title: Antitumor Efficacy of Human Monocyte-Derived Dendritic Cells: Comparing Effects of two Monocyte Isolation Methods

doi: 10.1186/s12575-018-0069-6

Figure Lengend Snippet: Comparison of MoDCs viability and maturation. After isolating monocytes with MACS or EasySep technologies, cells were cultured with GM-CSF and IL-4 during 1 week to allow their differentiation into MoDCs, as described in the Material and Methods section. MoDCs were collected and stained with different makers : 7AAD to assess cell viability ( a , b ); HLA-DR, a maturation marker ( c , d ); CD86, a co-stimulatory molecule ( e , f ). a, c, e – MoDCs derived from monocytes isolated with MACS technology; b , d , f – MoDCs derived from monocytes isolated with EasySep technology. Cells were analyzed by flow cytometry and the presented histograms refer to the population of stained MoDC (dark grey) and unstained control (light grey). A representative histogram of one out of eight different donors is shown

Article Snippet: Anti-CD86 antibody (ImmunoTools), and anti-HLA-DR (Immunostep; Salamanca, Spain) were used to assess MoDC maturation.

Techniques: Comparison, Cell Culture, Staining, Marker, Derivative Assay, Isolation, Flow Cytometry, Control

Journal: Biological Procedures Online

Article Title: Antitumor Efficacy of Human Monocyte-Derived Dendritic Cells: Comparing Effects of two Monocyte Isolation Methods

doi: 10.1186/s12575-018-0069-6

Figure Lengend Snippet: Comparison of activation level between MoDCs and responsiveness to maturation stimuli or tumor antigens. MACS_MoDCs (black bars, MACS) and EasySep_MoDCs (grey bars, EasySep) were loaded with tumor cell lysates (TL) from MCF-7 breast cancer cell line or stimulated with LPS and TNF-α during 4 h and analyzed by flow cytometry for the expression of ( a ) HLA-DR, and ( b ) CD86. Unstimulated MoDCs were used as controls. Data shown refer to mean ± SEM of MFI ( n = 3 different donors)

Article Snippet: Anti-CD86 antibody (ImmunoTools), and anti-HLA-DR (Immunostep; Salamanca, Spain) were used to assess MoDC maturation.

Techniques: Comparison, Activation Assay, Flow Cytometry, Expressing