Journal: Oxidative Medicine and Cellular Longevity

Article Title: Fibroblast Common Serum Response Signature-Related Classification Affects the Tumour Microenvironment and Predicts Prognosis in Bladder Cancer

doi: 10.1155/2022/5645944

Figure Lengend Snippet: ANLN to immunity in bladder cancer. (a) Boxplot of the abundance of PD-L1 expression in the CRS groups in the TCGA-BLCA cohort. (b) Correlation analysis between PD-L1 expression and the CRS in TCGA cohort. mRNA levels of (c) ANLN or (d) EML1 in anti-PD-L1 responsiveness in the IMvigor210 cohort. (e, f) Correlation analyses of expression of ANLN and CD8 and PD-L1 in TCGA cohort. (g) Survival probability of patients with differential expression of ANLN of TCGA and GSE13507 cohorts. Levels of ANLN were identified according to the median of ANLN. (h) Correlation analyses of ANLN expression and the CRS. (i) Correlation analyses of expression of ANLN and TNM staging. (j) Correlation analyses of expression of ANLN and grade. Correlation analyses between (k) number of CD8+ T cells or (l) PD-L1 expression and ANLN expression of patients with BLCA in our hospital cohort. (m) Representative IHC or HE images of ANLN, CD8, PD-L1, and Ki67. CR: complete response; PR: partial response; SD: stable disease; PD: progressive disease; CRS: fibroblast common serum response risk score.

Article Snippet: Antibodies against anillin (ANLN) (DF13590, 1 : 200; Affinity Biosciences), CD8 (PB9249, 1 : 200; BOSTER), and PD-L1 (66248-1-lg, 1 : 5000; Proteintech) were used for the immunohistochemical (IHC) staining of these proteins in the tissue samples, which was performed according to previously published methods [ ].

Techniques: Expressing, Quantitative Proteomics

Journal: Phytomedicine : international journal of phytotherapy and phytopharmacology

Article Title: Spatially resolved multi-omics reveals the renal cortex-metabolic reprogramming of Shenhua Tablet for intervention on IgA nephropathy.

doi: 10.1016/j.phymed.2025.156742

Figure Lengend Snippet: Fig. 2. SHT protects from renal dysfunction, mesangial cell proliferation and inflammatory responses in IgAN rats. (A) The 24 h Upro, Scr and BUN levels in the experimental groups (n = 8 in each group). (B) Representative images showing PCNA and PAS staining and semi-quantitative analysis of mesangial cell pro liferation in the experimental groups (n = 6 in each group). Scale bars, 20 μm. (C) Serum IL-6, IL-1β and TNF-α levels in the experimental groups (n = 8 in each group). (D) Immunohistochemical images and semi-quantitative analysis of OX8 and CD68 expression in the experimental groups. (n = 6 in each group). All sta tistical data are presented as mean ± SD and analyzed by ordinary one-way ANOVA followed by Tukey’s multiple comparison test. Comparative analysis, *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001, ns, not significant.

Article Snippet: The corresponding stain reagent was PAS Stain Kit (Solarbio, G1281), whereas the antibodies used included PCNA rabbit polyclonal antibody (1:200 dilution, Proteintech, 10,205–2-AP), Rabbit Anti-ODC1 antibody (1:200 dilution, Bioss, bs1294R), Col4 Polyclonal antibody (1:300 dilution, Bioss, bsm56208R), CD68 rabbit polyclonal antibody (1:200 dilution, Servicebio, GB113109), OX8 antibodies (1:200 dilution, boster, A02236–1), and HRP-conjugated goat anti-rabbit IgG (H + l) (1:200 dilution, ServiceBio, GB23303).

Techniques: Staining, Immunohistochemical staining, Expressing, Comparison

Journal: Annals of Translational Medicine

Article Title: Integrated analysis of immune infiltration in esophageal carcinoma as prognostic biomarkers

doi: 10.21037/atm-21-5881

Figure Lengend Snippet: Comparison of CIBERSORT immune fractions between ESCC tumor tissues and adjacent tissues

Article Snippet: IHC was performed according to manufacturer’s instructions with appropriate antibodies against tryptase (ab329383, diluted 1/1,000, Abcam, Cambridge, MA, USA), CD8 (ab329383, Abcam, diluted 1/1,000).

Techniques: Comparison

Journal: Annals of Translational Medicine

Article Title: Integrated analysis of immune infiltration in esophageal carcinoma as prognostic biomarkers

doi: 10.21037/atm-21-5881

Figure Lengend Snippet: Immunohistochemical staining analysis in ESCC tissues. (A) Mast cell number in ESCC TNM stages were analyzed. (B) The correlations between mast cells and CD8+ T cells in ESCC were analyzed. (C) Representative analysis of tryptase+ and CD8+ (brown) cells distributions in tumor tissues of ESCC patients by immunohistochemical staining. **P<0.01; *200: magnification of 200 times; *400: magnification of 400 times. TNM, tumor-node-metastasis; ESCC, esophageal squamous cell carcinoma.

Article Snippet: IHC was performed according to manufacturer’s instructions with appropriate antibodies against tryptase (ab329383, diluted 1/1,000, Abcam, Cambridge, MA, USA), CD8 (ab329383, Abcam, diluted 1/1,000).

Techniques: Immunohistochemical staining, Staining

Journal: Annals of Translational Medicine

Article Title: Integrated analysis of immune infiltration in esophageal carcinoma as prognostic biomarkers

doi: 10.21037/atm-21-5881

Figure Lengend Snippet: K-M survival analysis of OS and DFS according to mast cells and CD8+ T cells. K-M, Kaplan-Meier; OS, overall survival; DFS, disease-free survival.

Article Snippet: IHC was performed according to manufacturer’s instructions with appropriate antibodies against tryptase (ab329383, diluted 1/1,000, Abcam, Cambridge, MA, USA), CD8 (ab329383, Abcam, diluted 1/1,000).

Techniques:

Journal: Annals of Translational Medicine

Article Title: Integrated analysis of immune infiltration in esophageal carcinoma as prognostic biomarkers

doi: 10.21037/atm-21-5881

Figure Lengend Snippet: Correlation between mast cells, CD8 + T cells infiltration and patient characteristics

Article Snippet: IHC was performed according to manufacturer’s instructions with appropriate antibodies against tryptase (ab329383, diluted 1/1,000, Abcam, Cambridge, MA, USA), CD8 (ab329383, Abcam, diluted 1/1,000).

Techniques:

Journal: Annals of Translational Medicine

Article Title: Integrated analysis of immune infiltration in esophageal carcinoma as prognostic biomarkers

doi: 10.21037/atm-21-5881

Figure Lengend Snippet: Univariate and multivariate Cox regression analysis of overall survival

Article Snippet: IHC was performed according to manufacturer’s instructions with appropriate antibodies against tryptase (ab329383, diluted 1/1,000, Abcam, Cambridge, MA, USA), CD8 (ab329383, Abcam, diluted 1/1,000).

Techniques: