Journal: Stem cell research

Article Title: Derivation of keratinocytes from chicken embryonic stem cells: establishment and characterization of differentiated proliferative cell populations.

doi: 10.1016/j.scr.2015.01.002

Figure Lengend Snippet: Figure 7 Protein expression in K-cES cells and terminal differentiation. (A) Protein expression in K-cES-K1 cells analyzed by fluorescence microscopy. αK-11E10, type II keratins and involucrin expression (green) in fixed and permeabilized cells. Nuclei were stained with Hoechst 33342 dye (blue). Scale bars represent 50 μm. (B) 3-Dimension reconstructions of K-cES-K1 cells spontaneously differentiating in culture, stained with anti-involucrin (red) antibody and anti-CD44 antibody (green). Nuclei were stained with Hoechst 33342 dye (blue). K-cES cells showing relocalization of involucrin to the cell membrane are in the upper layer of the cell culture. (C) Spontaneous terminal differentiation of keratinocytes into corneocytes of K-cES cells. Corneocytes obtained from squamous cells floating in the culture medium harvested by centrifugation and heated at 100 °C in a 2% SDS–2% 2ME solution show conserved morphology with corneocytes from CPK.

Article Snippet: Antibodies to cytokeratin type II (1h5), mitochondria (4C7) and CD44 (1D10) were purchased from the Developmental Studies Hybridoma Bank (Iowa University, USA).

Techniques: Expressing, Fluorescence, Microscopy, Staining, Membrane, Cell Culture, Centrifugation

Journal: Phytomedicine

Article Title: Exploring the Therapeutic Potential of HAPC in COVID-19-Induced Acute Lung Injury

doi: 10.1016/j.phymed.2025.156563

Figure Lengend Snippet: Fig. 3. Optimization of chicoric acid to target CD44 using bioengineering nanospores. (A,B) Demonstration of an easy synthesis strategy for HAPC nano particles using an emulsion method. (C, D) Lactobacillus murinus conidia cell wall (CW). (E) Validation of the assembly of CW and HAPC nanoparticles via FRET. (F) Size distribution of HAPC nanoparticles. (G) Size distribution of optimized HAPC nanoparticles. H) Interaction between CW and PEI identified via FRET.

Article Snippet: HAPC–Cy5.5, 50-μg HAPC–Cy5.5 plus 100-μg HA, or 50-μg seng-cy5.5 and 5-μl anti-CD44 antibody in a serum-free medium for 2 h RAW 264.7 cells (1 × 105 cells/well) were treated with 100-ng/ml LPS and 10-ng/ml IFN-γ (HY-P7025, MCE) for 24 h to activate macrophages.

Techniques: Emulsion, Biomarker Discovery

Journal: Oncology Letters

Article Title: CD44 promotes the migration of bone marrow-derived mesenchymal stem cells toward glioma

doi: 10.3892/ol.2016.4270

Figure Lengend Snippet: PDGF-BB upregulates CD44 expression in BMSCs. (A) Reverse transcription-polymerase chain reaction was performed to evaluate the changes in CD44 mRNA expression levels under PDGF-BB conditions of 40 ng/ml or C6 glioma. (B) Results demonstrated that 40 ng/ml PDGF-BB and C6 glioma significantly elevated the CD44 expression of BMSCs compared with control groups (*P<0.01; **P<0.001). (C) Immunofluorescence revealing the effect of treatment with 40 ng/ml PDGF-BB on the protein levels of CD44 in BMSCs. Yellow staining indicates CD44 expression (magnification, x400; scale bar, 100 µm). PDGF-BB, platelet-derived growth factor-BB; CD, cluster of differentiation; BMSCs, bone marrow-derived mesenchymal stem cells; IDV, integrated density value.

Article Snippet: Upon blocking with 5% bovine serum albumin (Sigma-Aldrich) in PBS for 1 h, the cells were incubated with polyclonal rabbit anti-human/mouse/rat CD44 antibody (dilution, 1:100; catalog no., PA1021-2; Wuhan Boster Biological Technology, Ltd., Wuhan, China) for 4 h at room temperature, followed by incubation with rhodamine-conjugated goat anti-mouse immunoglobulin G secondary antibody (dilution, 1:100; catalog no., ZF-0313; Zhongshan Golden Bridge Biotechnology Co., Ltd, Beijing, China) for 1 h at room temperature.

Techniques: Expressing, Reverse Transcription, Polymerase Chain Reaction, Control, Immunofluorescence, Staining, Derivative Assay

Journal: Oncology Letters

Article Title: CD44 promotes the migration of bone marrow-derived mesenchymal stem cells toward glioma

doi: 10.3892/ol.2016.4270

Figure Lengend Snippet: Anti-CD44 antibody attenuates C6 cell-induced and PDGF-BB-promoted chemostatic migration of BMSCs. (A) The effect of CD44 in chemostatic migration of BMSCs was evaluated using OX-50, an anti-CD44 antibody. Representative images of each group are presented (magnification, x400; scale bar, 100 µm). (B) Quantification of the data corresponding to panel A. The graph represents the mean ± standard deviation of three independent experiments. *P<0.05 and **P<0.01 vs. control. CD, cluster of differentiation; PDGF-BB, platelet-derived growth factor-BB; BMSCs, bone marrow-derived mesenchymal stem cells.

Article Snippet: Upon blocking with 5% bovine serum albumin (Sigma-Aldrich) in PBS for 1 h, the cells were incubated with polyclonal rabbit anti-human/mouse/rat CD44 antibody (dilution, 1:100; catalog no., PA1021-2; Wuhan Boster Biological Technology, Ltd., Wuhan, China) for 4 h at room temperature, followed by incubation with rhodamine-conjugated goat anti-mouse immunoglobulin G secondary antibody (dilution, 1:100; catalog no., ZF-0313; Zhongshan Golden Bridge Biotechnology Co., Ltd, Beijing, China) for 1 h at room temperature.

Techniques: Migration, Standard Deviation, Control, Derivative Assay

Journal: Pharmaceuticals

Article Title: Multiple Antitumor Molecular Mechanisms Are Activated by a Fully Synthetic and Stabilized Pharmaceutical Product Delivering the Active Compound Sulforaphane (SFX-01) in Preclinical Model of Human Glioblastoma

doi: 10.3390/ph14111082

Figure Lengend Snippet: SFX-01 reduces PNT through the reduction of mesenchyme markers and induction of differentiated ones. ( A ) Representative western blots performed on U87MG cell extracts: Time-dependent modulation of CD44, Oct3/4 (mesenchymal/stem cell markers), β3-tubulin, nestin, and GFAP (differentiated markers). ( B ) Representative FACS analysis: Time-dependent modulation of CD44, Stro1, and CD90 as MES/stem cell markers; β3-tubulin and neurofilaments (NF200) as neural markers; and GFAP as an astrocyte marker. ( C ) semi-quantitative analyses (normalized densitometric values obtained by ImageJ in western blots performed on cell extracts from U87MG, U251, A172 and T98G glioma cells. ( D ) matrigel invasion assay (representative pictures from U87MG cells) and ( E ) counts of invaded cells/microscopic field in U87MG, U251, A172 and T98G cells treated with 5 and 10 µM SFX-01 ( F ) Analysis of vasculomimicry (VM) of U87MG (and U251, for comparison of more-differentiated cells) showed reduced Matrigel invasion by cells treated with 5 or 10 µM SFX-01. Pre-treatment with SFX-01 decreased VM activity. Quantification of VM was performed by the evaluation of tubule structure and branching index calculation.

Article Snippet: Antibodies against p21 (STJ94862), p27 (STJ94867), cyclin B1 (STJ97479), cyclin D1 (STJ92538), CDK4 (STJ11101180), CD44 (STJ119186), nestin (STJ113858), N-cadherin (STJ94353), catenin-beta (STJ92042), Bax (STJ118092), phospho-Akt (S473) (STJ90166), total Akt (STJ91538), 4E-BP1 (STJ91385), phospho-4E-BP1 (S65) (STJ90778), STAT3 (STJ95808), and phospho-STAT3 (Tyr 705 code STJ90411) were purchased from St John’s Laboratory Ltd. (Docklands Campus University Way, London, UK).

Techniques: Western Blot, Marker, Invasion Assay, Comparison, Activity Assay

Journal: Journal of cellular and molecular medicine

Article Title: Antineoplastic effects of clove buds (Syzygium aromaticum L.) in the model of breast carcinoma.

doi: 10.1111/jcmm.13197

Figure Lengend Snippet: Fig. 4 Representative images of caspase- 3, Bax, Bcl-2, Ki67, VEGFA, VEGFR-2, MDA, CD24, CD44, ALDH1A1, H3K4 m3, H3K9 m3, H4K20 m3, H4K16ac expres- sions in rat mammary carcinoma cells. For detection, polyclonal caspase-3 anti- body (Bioss, Woburn, USA), polyclonal Bax and Bcl-2 antibodies (Santa Cruz Biotechnology), monoclonal Ki67 antibody (Dako), monoclonal VEGFA and VEGFR-2 antibodies (Santa Cruz Biotechnology), polyclonal CD24 antibody (GeneTex), poly- clonal CD44 antibody (Boster), polyclonal ALDH1A1 antibody (Thermo Fisher), polyclonal EpCAM antibody (Abcam) poly- clonal H3K4 m, H3K9 m3 and H4K20 m3 antibodies (Abcam) and monoclonal H4K16ac antibody (Abcam) were used; final magnifications: 9400.

Article Snippet: For detection, polyclonal caspase-3 antibody (Bioss, Woburn, USA), polyclonal Bax and Bcl-2 antibodies (Santa Cruz Biotechnology), monoclonal Ki67 antibody (Dako), monoclonal VEGFA and VEGFR-2 antibodies (Santa Cruz Biotechnology), polyclonal CD24 antibody (GeneTex), polyclonal CD44 antibody (Boster), polyclonal ALDH1A1 antibody (Thermo Fisher), polyclonal EpCAM antibody (Abcam) polyclonal H3K4 m, H3K9 m3 and H4K20 m3 antibodies (Abcam) and monoclonal H4K16ac antibody (Abcam) were used; final magnifications: 9400. a 2017 The Authors.

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