Journal: Journal of immunology (Baltimore, Md. : 1950)

Article Title: Dual function of CD70 in viral infection: modulator of early cytokine responses and activator of adaptive responses 1

doi: 10.4049/jimmunol.1302429

Figure Lengend Snippet: (A-J) WT and CD70-/- mice were injected with an isotype control Ab (Iso) or depleted of CD4+CD25+Foxp3+ cells with anti-CD25 Ab then infected with MCMV. Mice were analyzed 36 h p.i. (A, F) Percentages of Treg in spleens. (B, G) Serum levels of IFN-α, IL-12p70 and TNF-α. (C, H) CD69 expression on NK cells and frequencies of IFN-γ-producing NK cells in spleens. (D, I) Frequencies of splenic NK cells and (E, J) serum IFN-γ levels. Data are combined from two independent experiments. Statistical significance is indicated by p values. (K) Treg were isolated from naïve WT and CD70-/- mice and co-cultured with CFSE-labeled naïve CD4+CD25- T cells (Tconv) from WT mice for 3 d. Proliferation was measured by flow cytometry. Numbers indicate percentages of Tconv that divided two times or more.

Article Snippet: To deplete Treg with antibody, C57BL/6 and CD70 -/- mice were injected i.p. with 300-500 μg of anti-CD25 Ab (clone PC-61.5.3, Bio X Cell) or a rat IgG isotype control Ab on days -3 and -1 of infection.

Techniques: Injection, Infection, Expressing, Isolation, Cell Culture, Labeling, Flow Cytometry

Journal: Human Vaccines & Immunotherapeutics

Article Title: Research and development of mAb drugs in China: a look from policy perspectives

doi: 10.1080/21645515.2019.1605809

Figure Lengend Snippet: Domestically approved mAb drugs by 2017.

Article Snippet: , Jiannipai , Recombinant Humanized Anti-CD25 mAbs Injection , Shanghai CP Guojian Pharmaceutical Co., Ltd. , Humanzied , 2011 , Transplant Rejection.

Techniques: Injection, Cream, Recombinant

Journal: Journal of Translational Medicine

Article Title: Lactobacillus paracasei R3 protects against dextran sulfate sodium (DSS)-induced colitis in mice via regulating Th17/Treg cell balance

doi: 10.1186/s12967-021-02943-x

Figure Lengend Snippet: L.p R3 attenuates colonic inflammatory responses but induces Treg responses in DSS-induced experimental murine colitis. A The expression of Foxp3, IL-10, IL-17A and IFN-γ were detected in the colon by the immunohistochemical method. B Pathological score of Foxp3, IL-10, IL-17A and IFN-γ expression in colon tissue. C Evaluate the expression of CD4, CD25, Foxp3, IL-17A, IFN-γ, IL-10 in colon tissue by Western blot. *p < 0.05; **p < 0.01; ***p < 0.001

Article Snippet: The tissue was homogenized with lysis buffer, an equal amount of protein (50 μg) was analyzed by 12% SDS–polyacrylamide gel electrophoresis, and the blotted membrane was blocked with 5% BSA in TBST for 60 min at room temperature, anti-Foxp3 (1:1000, Abcam), anti-CD25 (1:1000, Affinity Biosciences), anti-IL-17A (1:1000, Affinity Biosciences), anti-L-10 (1:1000, bs-6761R, Bioss) and FN-γ (1:1000, Affinity Biosciences) antibody overnight at 4 °C.

Techniques: Expressing, Immunohistochemistry, Western Blot