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Image Search Results
Journal: PLoS ONE
Article Title: Efficient, Long Term Production of Monocyte-Derived Macrophages from Human Pluripotent Stem Cells under Partly-Defined and Fully-Defined Conditions
doi: 10.1371/journal.pone.0071098
Figure Lengend Snippet: A) Morphology of MC. From left to right: Eosin-methylene blue staining of MC from cytospins, Scanning Electron Microscopy, and Transmission Electron Microscopy. Scale bar, 10 µM. D) Diameter of PSC-MC (n = 7) and b-MC (n = 3) were determined by an AO-DAPI stain (Chemometec). Bars represent the mean diameter ± SEM. C) Phenotype of MC. Surface expression of CD14, CD16, CD163, CD86 and MHC II were measured by flow cytometry. Histograms represent surface staining (black line) compared to the isotype control (shaded gray). D) The symbols reflect the relative ratio of the geometric mean fluorescence intensity (MFI) over the isotype control of independent experiments.
Article Snippet: The following antibodies (clone, isotype controls, supplier) were used: CD34-APC (4H1, IgG1κ -APC, eBioscience), CD38-PE (IB6, IgG2b-PE, Miltenyi Biotec Ltd), Thy-1-PE (5E10, IgG1κ-PE, BD), CD14-APC (MEM-15, IgG1-APC, Immunotools), CD45 (MEM-28, IgG1-APC, Immunotools),
Techniques: Staining, Electron Microscopy, Transmission Assay, Expressing, Flow Cytometry, Control, Fluorescence
Journal: PLoS ONE
Article Title: Efficient, Long Term Production of Monocyte-Derived Macrophages from Human Pluripotent Stem Cells under Partly-Defined and Fully-Defined Conditions
doi: 10.1371/journal.pone.0071098
Figure Lengend Snippet: A) Morphology of MDM. From left to right: Representative brightfield image (scale bare 200 µM), Scanning Electron Microscopy and Transmission Electron Microscopy (scale bar, 10 µM) D) Diameter of PSC-MDM (n = 3) and b-MDM (n = 6) were determined by an AO-DAPI stain (Chemometec). Bars represent the mean diameter ± SEM. C) Phenotype of MC. Surface expression of CD14, CD16, CD163, CD86 and MHC II were measured by flow cytometry. Histograms represent surface staining (black line) compared to the isotype control (shaded gray). D) The symbols reflect the relative ratio of the geometric mean fluorescence intensity (MFI) over the isotype control of independent experiments.
Article Snippet: The following antibodies (clone, isotype controls, supplier) were used: CD34-APC (4H1, IgG1κ -APC, eBioscience), CD38-PE (IB6, IgG2b-PE, Miltenyi Biotec Ltd), Thy-1-PE (5E10, IgG1κ-PE, BD), CD14-APC (MEM-15, IgG1-APC, Immunotools), CD45 (MEM-28, IgG1-APC, Immunotools),
Techniques: Electron Microscopy, Transmission Assay, Staining, Expressing, Flow Cytometry, Control, Fluorescence