anti-cd127 Search Results


cd127 pe  (Miltenyi Biotec)
93
Miltenyi Biotec cd127 pe
Variations (fold-change) in circulating Treg cells and grass allergen-specific IL-10-producing cells after four months of treatment depending on dose and route of administration (subcutaneous: left panels; sublingual: right panels) (A) Percentage of Treg cells (CD4 + <t>CD127</t> - CD25 + FOXP3 + ) relative to total CD4 + T cells. (B) Number of Phleum-specific spot-forming cells (SFC) secreting IL-10 after their expansion in vitro . (C) Data as in panel B but regrouping of subjects as indicated. Results are expressed as fold-change (median; interquartile range) relative to baseline. Comparison with placebo, Unpaired T test or Mann-Whitney test: * p<0.05.
Cd127 Pe, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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anti il7rα a7r34 antibody  (Bio X Cell)
93
Bio X Cell anti il7rα a7r34 antibody
(A) Representative intracellular flow cytometry plots of IFNγ- and IL17-producing CD4+ T cells in the PLN (numbers represent percentages of gated populations). (B–C) Frequencies and total cell numbers of CD4+IL17+ (B) and CD4+IFNγ+ (C) subsets impacted by CTLA4RNAi on the B6.H2g7 background. Control mice were transgene-negative littermates (n=4–6 per group). (D) Littermate CTLA4RNAi/B6.H2g7 mice were treated with non-specific ratIgG2a control antibody or <t>anti-IL7Rα</t> antibody for 3 weeks. Mice were monitored for diabetes for 40 days. (E) Representative intracellular flow cytometry plots of the pancreas showing IL17- and IFNγ-production by CD4+ T cells after anti-IL7Rα treatment (numbers represent percentage of gated populations). (F–G) Frequencies and total cell numbers of CD4+IL17+(F) and CD4+IFNγ+ (G) after anti-IL7Rα treatment (n=4–6 mice per group). Each data-point represents one animal (Mean ± SEM). *p<0.05; **p<0.01; ***p<0.005; ns, not significant.
Anti Il7rα A7r34 Antibody, supplied by Bio X Cell, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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3143012b  (fluidigm)
94
fluidigm 3143012b
(A) Representative intracellular flow cytometry plots of IFNγ- and IL17-producing CD4+ T cells in the PLN (numbers represent percentages of gated populations). (B–C) Frequencies and total cell numbers of CD4+IL17+ (B) and CD4+IFNγ+ (C) subsets impacted by CTLA4RNAi on the B6.H2g7 background. Control mice were transgene-negative littermates (n=4–6 per group). (D) Littermate CTLA4RNAi/B6.H2g7 mice were treated with non-specific ratIgG2a control antibody or <t>anti-IL7Rα</t> antibody for 3 weeks. Mice were monitored for diabetes for 40 days. (E) Representative intracellular flow cytometry plots of the pancreas showing IL17- and IFNγ-production by CD4+ T cells after anti-IL7Rα treatment (numbers represent percentage of gated populations). (F–G) Frequencies and total cell numbers of CD4+IL17+(F) and CD4+IFNγ+ (G) after anti-IL7Rα treatment (n=4–6 mice per group). Each data-point represents one animal (Mean ± SEM). *p<0.05; **p<0.01; ***p<0.005; ns, not significant.
3143012b, supplied by fluidigm, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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3165008b 166 er cd25  (fluidigm)
94
fluidigm 3165008b 166 er cd25
(A) Representative intracellular flow cytometry plots of IFNγ- and IL17-producing CD4+ T cells in the PLN (numbers represent percentages of gated populations). (B–C) Frequencies and total cell numbers of CD4+IL17+ (B) and CD4+IFNγ+ (C) subsets impacted by CTLA4RNAi on the B6.H2g7 background. Control mice were transgene-negative littermates (n=4–6 per group). (D) Littermate CTLA4RNAi/B6.H2g7 mice were treated with non-specific ratIgG2a control antibody or <t>anti-IL7Rα</t> antibody for 3 weeks. Mice were monitored for diabetes for 40 days. (E) Representative intracellular flow cytometry plots of the pancreas showing IL17- and IFNγ-production by CD4+ T cells after anti-IL7Rα treatment (numbers represent percentage of gated populations). (F–G) Frequencies and total cell numbers of CD4+IL17+(F) and CD4+IFNγ+ (G) after anti-IL7Rα treatment (n=4–6 mice per group). Each data-point represents one animal (Mean ± SEM). *p<0.05; **p<0.01; ***p<0.005; ns, not significant.
3165008b 166 Er Cd25, supplied by fluidigm, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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pe cy7 anti cd127  (Elabscience Biotechnology)
92
Elabscience Biotechnology pe cy7 anti cd127
(A) Representative intracellular flow cytometry plots of IFNγ- and IL17-producing CD4+ T cells in the PLN (numbers represent percentages of gated populations). (B–C) Frequencies and total cell numbers of CD4+IL17+ (B) and CD4+IFNγ+ (C) subsets impacted by CTLA4RNAi on the B6.H2g7 background. Control mice were transgene-negative littermates (n=4–6 per group). (D) Littermate CTLA4RNAi/B6.H2g7 mice were treated with non-specific ratIgG2a control antibody or <t>anti-IL7Rα</t> antibody for 3 weeks. Mice were monitored for diabetes for 40 days. (E) Representative intracellular flow cytometry plots of the pancreas showing IL17- and IFNγ-production by CD4+ T cells after anti-IL7Rα treatment (numbers represent percentage of gated populations). (F–G) Frequencies and total cell numbers of CD4+IL17+(F) and CD4+IFNγ+ (G) after anti-IL7Rα treatment (n=4–6 mice per group). Each data-point represents one animal (Mean ± SEM). *p<0.05; **p<0.01; ***p<0.005; ns, not significant.
Pe Cy7 Anti Cd127, supplied by Elabscience Biotechnology, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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cd127 fitc  (Elabscience Biotechnology)
91
Elabscience Biotechnology cd127 fitc
(A) Representative intracellular flow cytometry plots of IFNγ- and IL17-producing CD4+ T cells in the PLN (numbers represent percentages of gated populations). (B–C) Frequencies and total cell numbers of CD4+IL17+ (B) and CD4+IFNγ+ (C) subsets impacted by CTLA4RNAi on the B6.H2g7 background. Control mice were transgene-negative littermates (n=4–6 per group). (D) Littermate CTLA4RNAi/B6.H2g7 mice were treated with non-specific ratIgG2a control antibody or <t>anti-IL7Rα</t> antibody for 3 weeks. Mice were monitored for diabetes for 40 days. (E) Representative intracellular flow cytometry plots of the pancreas showing IL17- and IFNγ-production by CD4+ T cells after anti-IL7Rα treatment (numbers represent percentage of gated populations). (F–G) Frequencies and total cell numbers of CD4+IL17+(F) and CD4+IFNγ+ (G) after anti-IL7Rα treatment (n=4–6 mice per group). Each data-point represents one animal (Mean ± SEM). *p<0.05; **p<0.01; ***p<0.005; ns, not significant.
Cd127 Fitc, supplied by Elabscience Biotechnology, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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cd127  (Cytek Biosciences)
90
Cytek Biosciences cd127
(A) Representative intracellular flow cytometry plots of IFNγ- and IL17-producing CD4+ T cells in the PLN (numbers represent percentages of gated populations). (B–C) Frequencies and total cell numbers of CD4+IL17+ (B) and CD4+IFNγ+ (C) subsets impacted by CTLA4RNAi on the B6.H2g7 background. Control mice were transgene-negative littermates (n=4–6 per group). (D) Littermate CTLA4RNAi/B6.H2g7 mice were treated with non-specific ratIgG2a control antibody or <t>anti-IL7Rα</t> antibody for 3 weeks. Mice were monitored for diabetes for 40 days. (E) Representative intracellular flow cytometry plots of the pancreas showing IL17- and IFNγ-production by CD4+ T cells after anti-IL7Rα treatment (numbers represent percentage of gated populations). (F–G) Frequencies and total cell numbers of CD4+IL17+(F) and CD4+IFNγ+ (G) after anti-IL7Rα treatment (n=4–6 mice per group). Each data-point represents one animal (Mean ± SEM). *p<0.05; **p<0.01; ***p<0.005; ns, not significant.
Cd127, supplied by Cytek Biosciences, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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e ab f1152e  (Elabscience Biotechnology)
94
Elabscience Biotechnology e ab f1152e
(A) Representative intracellular flow cytometry plots of IFNγ- and IL17-producing CD4+ T cells in the PLN (numbers represent percentages of gated populations). (B–C) Frequencies and total cell numbers of CD4+IL17+ (B) and CD4+IFNγ+ (C) subsets impacted by CTLA4RNAi on the B6.H2g7 background. Control mice were transgene-negative littermates (n=4–6 per group). (D) Littermate CTLA4RNAi/B6.H2g7 mice were treated with non-specific ratIgG2a control antibody or <t>anti-IL7Rα</t> antibody for 3 weeks. Mice were monitored for diabetes for 40 days. (E) Representative intracellular flow cytometry plots of the pancreas showing IL17- and IFNγ-production by CD4+ T cells after anti-IL7Rα treatment (numbers represent percentage of gated populations). (F–G) Frequencies and total cell numbers of CD4+IL17+(F) and CD4+IFNγ+ (G) after anti-IL7Rα treatment (n=4–6 mice per group). Each data-point represents one animal (Mean ± SEM). *p<0.05; **p<0.01; ***p<0.005; ns, not significant.
E Ab F1152e, supplied by Elabscience Biotechnology, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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il7ra  (Cytek Biosciences)
90
Cytek Biosciences il7ra
(A) Representative intracellular flow cytometry plots of IFNγ- and IL17-producing CD4+ T cells in the PLN (numbers represent percentages of gated populations). (B–C) Frequencies and total cell numbers of CD4+IL17+ (B) and CD4+IFNγ+ (C) subsets impacted by CTLA4RNAi on the B6.H2g7 background. Control mice were transgene-negative littermates (n=4–6 per group). (D) Littermate CTLA4RNAi/B6.H2g7 mice were treated with non-specific ratIgG2a control antibody or <t>anti-IL7Rα</t> antibody for 3 weeks. Mice were monitored for diabetes for 40 days. (E) Representative intracellular flow cytometry plots of the pancreas showing IL17- and IFNγ-production by CD4+ T cells after anti-IL7Rα treatment (numbers represent percentage of gated populations). (F–G) Frequencies and total cell numbers of CD4+IL17+(F) and CD4+IFNγ+ (G) after anti-IL7Rα treatment (n=4–6 mice per group). Each data-point represents one animal (Mean ± SEM). *p<0.05; **p<0.01; ***p<0.005; ns, not significant.
Il7ra, supplied by Cytek Biosciences, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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anti human cd127 a019d5 176yb  (fluidigm)
94
fluidigm anti human cd127 a019d5 176yb
(A) Representative intracellular flow cytometry plots of IFNγ- and IL17-producing CD4+ T cells in the PLN (numbers represent percentages of gated populations). (B–C) Frequencies and total cell numbers of CD4+IL17+ (B) and CD4+IFNγ+ (C) subsets impacted by CTLA4RNAi on the B6.H2g7 background. Control mice were transgene-negative littermates (n=4–6 per group). (D) Littermate CTLA4RNAi/B6.H2g7 mice were treated with non-specific ratIgG2a control antibody or <t>anti-IL7Rα</t> antibody for 3 weeks. Mice were monitored for diabetes for 40 days. (E) Representative intracellular flow cytometry plots of the pancreas showing IL17- and IFNγ-production by CD4+ T cells after anti-IL7Rα treatment (numbers represent percentage of gated populations). (F–G) Frequencies and total cell numbers of CD4+IL17+(F) and CD4+IFNγ+ (G) after anti-IL7Rα treatment (n=4–6 mice per group). Each data-point represents one animal (Mean ± SEM). *p<0.05; **p<0.01; ***p<0.005; ns, not significant.
Anti Human Cd127 A019d5 176yb, supplied by fluidigm, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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cd127  (Bio-Rad)
88
Bio-Rad cd127
Figure 3. CD4+ T cells in the pancreas show increased proliferation in anti-PD-1-induced T1D. (A) UMAP visualization of integrated CD4+ T cells detected in the pancreas. Points represent individual cells and colors denote cluster classification as labeled. (B) UMAP visualization of integrated CD4+ T cells detected in the pancreas of each treatment group. Dots represent individual cells with the color representing the cluster classification and size corresponding to the size of the clonotype. Colors denote functional annotations that correspond to the same labels shown in A. (C) Stacked bar plots denoting the percentage of cells from each treatment group within individual clusters (left panel). Heatmap showing the number of cells detected in each cluster and treatment group (middle panel) and −log10 adjusted P values from a Fisher’s exact test (right panel). (D) Violin plots quantifying percent TIGIT+ staining within CD4+FoxP3+ Treg in the pancreas measured by flow cytometry. IgG-Spt, P = 0.0235. (E) Violin plots showing gMFI of T-bet in CD4+FoxP3−CD11ahiCD62L−Tcon in the pancreas by flow cytometry analysis. IgG-PD1, P = 0.0008; Spt-PD1, P = 0.0020. (F) Violin plots quantifying percent <t>CD127+</t> staining within CD4+FoxP3−CD11ahiCD62L−Tcon in the pancreas measured by flow cytometry. IgG-Spt, P < 0.0001; Spt-PD1, P = 0.0012. (G) Violin plots showing percent BCL-2+ in CD4+FoxP3−CD11ahiCD62L−
Cd127, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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anti mouse cd127 il7ra  (fluidigm)
93
fluidigm anti mouse cd127 il7ra

Anti Mouse Cd127 Il7ra, supplied by fluidigm, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Variations (fold-change) in circulating Treg cells and grass allergen-specific IL-10-producing cells after four months of treatment depending on dose and route of administration (subcutaneous: left panels; sublingual: right panels) (A) Percentage of Treg cells (CD4 + CD127 - CD25 + FOXP3 + ) relative to total CD4 + T cells. (B) Number of Phleum-specific spot-forming cells (SFC) secreting IL-10 after their expansion in vitro . (C) Data as in panel B but regrouping of subjects as indicated. Results are expressed as fold-change (median; interquartile range) relative to baseline. Comparison with placebo, Unpaired T test or Mann-Whitney test: * p<0.05.

Journal: Frontiers in Immunology

Article Title: Grass pollen allergoids conjugated with mannan for subcutaneous and sublingual immunotherapy: a dose-finding study

doi: 10.3389/fimmu.2024.1431351

Figure Lengend Snippet: Variations (fold-change) in circulating Treg cells and grass allergen-specific IL-10-producing cells after four months of treatment depending on dose and route of administration (subcutaneous: left panels; sublingual: right panels) (A) Percentage of Treg cells (CD4 + CD127 - CD25 + FOXP3 + ) relative to total CD4 + T cells. (B) Number of Phleum-specific spot-forming cells (SFC) secreting IL-10 after their expansion in vitro . (C) Data as in panel B but regrouping of subjects as indicated. Results are expressed as fold-change (median; interquartile range) relative to baseline. Comparison with placebo, Unpaired T test or Mann-Whitney test: * p<0.05.

Article Snippet: For Treg cell analysis, PBMC were first subjected to surface staining with anti-human CD4-PerCP, CD127-PE, and CD25-APC (Miltenyi Biotec).

Techniques: In Vitro, Comparison, MANN-WHITNEY

(A) Representative intracellular flow cytometry plots of IFNγ- and IL17-producing CD4+ T cells in the PLN (numbers represent percentages of gated populations). (B–C) Frequencies and total cell numbers of CD4+IL17+ (B) and CD4+IFNγ+ (C) subsets impacted by CTLA4RNAi on the B6.H2g7 background. Control mice were transgene-negative littermates (n=4–6 per group). (D) Littermate CTLA4RNAi/B6.H2g7 mice were treated with non-specific ratIgG2a control antibody or anti-IL7Rα antibody for 3 weeks. Mice were monitored for diabetes for 40 days. (E) Representative intracellular flow cytometry plots of the pancreas showing IL17- and IFNγ-production by CD4+ T cells after anti-IL7Rα treatment (numbers represent percentage of gated populations). (F–G) Frequencies and total cell numbers of CD4+IL17+(F) and CD4+IFNγ+ (G) after anti-IL7Rα treatment (n=4–6 mice per group). Each data-point represents one animal (Mean ± SEM). *p<0.05; **p<0.01; ***p<0.005; ns, not significant.

Journal: Journal of immunology (Baltimore, Md. : 1950)

Article Title: Opposing Effects of CTLA4 Insufficiency on Regulatory versus Conventional T Cells in Autoimmunity Converge on Effector Memory in Target Tissue

doi: 10.4049/jimmunol.1400876

Figure Lengend Snippet: (A) Representative intracellular flow cytometry plots of IFNγ- and IL17-producing CD4+ T cells in the PLN (numbers represent percentages of gated populations). (B–C) Frequencies and total cell numbers of CD4+IL17+ (B) and CD4+IFNγ+ (C) subsets impacted by CTLA4RNAi on the B6.H2g7 background. Control mice were transgene-negative littermates (n=4–6 per group). (D) Littermate CTLA4RNAi/B6.H2g7 mice were treated with non-specific ratIgG2a control antibody or anti-IL7Rα antibody for 3 weeks. Mice were monitored for diabetes for 40 days. (E) Representative intracellular flow cytometry plots of the pancreas showing IL17- and IFNγ-production by CD4+ T cells after anti-IL7Rα treatment (numbers represent percentage of gated populations). (F–G) Frequencies and total cell numbers of CD4+IL17+(F) and CD4+IFNγ+ (G) after anti-IL7Rα treatment (n=4–6 mice per group). Each data-point represents one animal (Mean ± SEM). *p<0.05; **p<0.01; ***p<0.005; ns, not significant.

Article Snippet: For anti-IL7Rα antibody treatment, CTLA4RNAi/B6.H2 g7 mice were intraperitoneally administered with anti-IL7Rα (A7R34) antibody or Rat IgG1 isotype control antibody (BioXcell, West Lebanon, NH) at a dose of 25ug/g body weight beginning at 3–4 days of age, twice a week for 3 weeks.

Techniques: Flow Cytometry, Control

Figure 3. CD4+ T cells in the pancreas show increased proliferation in anti-PD-1-induced T1D. (A) UMAP visualization of integrated CD4+ T cells detected in the pancreas. Points represent individual cells and colors denote cluster classification as labeled. (B) UMAP visualization of integrated CD4+ T cells detected in the pancreas of each treatment group. Dots represent individual cells with the color representing the cluster classification and size corresponding to the size of the clonotype. Colors denote functional annotations that correspond to the same labels shown in A. (C) Stacked bar plots denoting the percentage of cells from each treatment group within individual clusters (left panel). Heatmap showing the number of cells detected in each cluster and treatment group (middle panel) and −log10 adjusted P values from a Fisher’s exact test (right panel). (D) Violin plots quantifying percent TIGIT+ staining within CD4+FoxP3+ Treg in the pancreas measured by flow cytometry. IgG-Spt, P = 0.0235. (E) Violin plots showing gMFI of T-bet in CD4+FoxP3−CD11ahiCD62L−Tcon in the pancreas by flow cytometry analysis. IgG-PD1, P = 0.0008; Spt-PD1, P = 0.0020. (F) Violin plots quantifying percent CD127+ staining within CD4+FoxP3−CD11ahiCD62L−Tcon in the pancreas measured by flow cytometry. IgG-Spt, P < 0.0001; Spt-PD1, P = 0.0012. (G) Violin plots showing percent BCL-2+ in CD4+FoxP3−CD11ahiCD62L−

Journal: The Journal of experimental medicine

Article Title: Single-cell profiling reveals unique features of diabetogenic T cells in anti-PD-1-induced type 1 diabetes mice.

doi: 10.1084/jem.20221920

Figure Lengend Snippet: Figure 3. CD4+ T cells in the pancreas show increased proliferation in anti-PD-1-induced T1D. (A) UMAP visualization of integrated CD4+ T cells detected in the pancreas. Points represent individual cells and colors denote cluster classification as labeled. (B) UMAP visualization of integrated CD4+ T cells detected in the pancreas of each treatment group. Dots represent individual cells with the color representing the cluster classification and size corresponding to the size of the clonotype. Colors denote functional annotations that correspond to the same labels shown in A. (C) Stacked bar plots denoting the percentage of cells from each treatment group within individual clusters (left panel). Heatmap showing the number of cells detected in each cluster and treatment group (middle panel) and −log10 adjusted P values from a Fisher’s exact test (right panel). (D) Violin plots quantifying percent TIGIT+ staining within CD4+FoxP3+ Treg in the pancreas measured by flow cytometry. IgG-Spt, P = 0.0235. (E) Violin plots showing gMFI of T-bet in CD4+FoxP3−CD11ahiCD62L−Tcon in the pancreas by flow cytometry analysis. IgG-PD1, P = 0.0008; Spt-PD1, P = 0.0020. (F) Violin plots quantifying percent CD127+ staining within CD4+FoxP3−CD11ahiCD62L−Tcon in the pancreas measured by flow cytometry. IgG-Spt, P < 0.0001; Spt-PD1, P = 0.0012. (G) Violin plots showing percent BCL-2+ in CD4+FoxP3−CD11ahiCD62L−

Article Snippet: Cells were preincubated with TruStain Fc Receptor Block (anti-mouse CD16/ CD32, clone 93; BioLegend) and then labeled with extracellular antibodies from BioLegend: CD8α (53-6.7), PD-1 (RMP1-30), CD29 (HMβ 1-1; BioLegend), CD48 (HM48-1), CD94 (18d3), NKG2D (CX5), CD39 (Duha59), NKG2A (16A11), TIM-3 (RMT323), SLAMF7 (4G2), TIGIT (IG9), CX3CR1 (SA011F11), CD62L (MEL-14), CD44 (IM7), CD127 (A7R34), CD49D (R1-2), CD38 (90), CXCR3 (CXCR3-173); BD Biosciences: TCR-β (H57-597), CD4 (GK1.5), CD11a (M17/4), CD8b (H35-17.2); and Bio-Rad: LAG-3 (C9B7W).

Techniques: Labeling, Functional Assay, Staining, Flow Cytometry

Journal: iScience

Article Title: From pseudo to real-time dynamics of T cell thymic differentiation

doi: 10.1016/j.isci.2022.105826

Figure Lengend Snippet:

Article Snippet: Anti-Mouse CD127 (IL7Ra) 175Lu , FLUIDIGM , Cat: #3175006; RRID: AB_2927570.

Techniques: Recombinant, Conjugation Assay, Software