Journal: Frontiers in Immunology

Article Title: Grass pollen allergoids conjugated with mannan for subcutaneous and sublingual immunotherapy: a dose-finding study

doi: 10.3389/fimmu.2024.1431351

Figure Lengend Snippet: Variations (fold-change) in circulating Treg cells and grass allergen-specific IL-10-producing cells after four months of treatment depending on dose and route of administration (subcutaneous: left panels; sublingual: right panels) (A) Percentage of Treg cells (CD4 + CD127 - CD25 + FOXP3 + ) relative to total CD4 + T cells. (B) Number of Phleum-specific spot-forming cells (SFC) secreting IL-10 after their expansion in vitro . (C) Data as in panel B but regrouping of subjects as indicated. Results are expressed as fold-change (median; interquartile range) relative to baseline. Comparison with placebo, Unpaired T test or Mann-Whitney test: * p<0.05.

Article Snippet: For Treg cell analysis, PBMC were first subjected to surface staining with anti-human CD4-PerCP, CD127-PE, and CD25-APC (Miltenyi Biotec).

Techniques: In Vitro, Comparison, MANN-WHITNEY

Journal: The Journal of experimental medicine

Article Title: Single-cell profiling reveals unique features of diabetogenic T cells in anti-PD-1-induced type 1 diabetes mice.

doi: 10.1084/jem.20221920

Figure Lengend Snippet: Figure 3. CD4+ T cells in the pancreas show increased proliferation in anti-PD-1-induced T1D. (A) UMAP visualization of integrated CD4+ T cells detected in the pancreas. Points represent individual cells and colors denote cluster classification as labeled. (B) UMAP visualization of integrated CD4+ T cells detected in the pancreas of each treatment group. Dots represent individual cells with the color representing the cluster classification and size corresponding to the size of the clonotype. Colors denote functional annotations that correspond to the same labels shown in A. (C) Stacked bar plots denoting the percentage of cells from each treatment group within individual clusters (left panel). Heatmap showing the number of cells detected in each cluster and treatment group (middle panel) and −log10 adjusted P values from a Fisher’s exact test (right panel). (D) Violin plots quantifying percent TIGIT+ staining within CD4+FoxP3+ Treg in the pancreas measured by flow cytometry. IgG-Spt, P = 0.0235. (E) Violin plots showing gMFI of T-bet in CD4+FoxP3−CD11ahiCD62L−Tcon in the pancreas by flow cytometry analysis. IgG-PD1, P = 0.0008; Spt-PD1, P = 0.0020. (F) Violin plots quantifying percent CD127+ staining within CD4+FoxP3−CD11ahiCD62L−Tcon in the pancreas measured by flow cytometry. IgG-Spt, P < 0.0001; Spt-PD1, P = 0.0012. (G) Violin plots showing percent BCL-2+ in CD4+FoxP3−CD11ahiCD62L−

Article Snippet: Cells were preincubated with TruStain Fc Receptor Block (anti-mouse CD16/ CD32, clone 93; BioLegend) and then labeled with extracellular antibodies from BioLegend: CD8α (53-6.7), PD-1 (RMP1-30), CD29 (HMβ 1-1; BioLegend), CD48 (HM48-1), CD94 (18d3), NKG2D (CX5), CD39 (Duha59), NKG2A (16A11), TIM-3 (RMT323), SLAMF7 (4G2), TIGIT (IG9), CX3CR1 (SA011F11), CD62L (MEL-14), CD44 (IM7), CD127 (A7R34), CD49D (R1-2), CD38 (90), CXCR3 (CXCR3-173); BD Biosciences: TCR-β (H57-597), CD4 (GK1.5), CD11a (M17/4), CD8b (H35-17.2); and Bio-Rad: LAG-3 (C9B7W).

Techniques: Labeling, Functional Assay, Staining, Flow Cytometry

Journal: iScience

Article Title: From pseudo to real-time dynamics of T cell thymic differentiation

doi: 10.1016/j.isci.2022.105826

Figure Lengend Snippet:

Article Snippet: Anti-Mouse CD127 (IL7Ra) 175Lu , FLUIDIGM , Cat: #3175006; RRID: AB_2927570.

Techniques: Recombinant, Conjugation Assay, Software

Journal: Cell Reports Medicine

Article Title: High-dimensional profiling of pediatric immune responses to solid organ transplantation

doi: 10.1016/j.xcrm.2023.101147

Figure Lengend Snippet: Antibodies used for CyTOF staining

Article Snippet: CD127 , 143-Nd , A019D5 , Standard Biotools , 3143012B.

Techniques:

Journal: Cell Reports Medicine

Article Title: High-dimensional profiling of pediatric immune responses to solid organ transplantation

doi: 10.1016/j.xcrm.2023.101147

Figure Lengend Snippet:

Article Snippet: CD127 , 143-Nd , A019D5 , Standard Biotools , 3143012B.

Techniques: Recombinant, Antibody Labeling, Staining, Blocking Assay, Software