anti-ca9 Search Results


hif-1 alpha antibody - bsa free  (Bio-Techne corporation)
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Hif 1 Alpha Antibody Bsa Free, supplied by Bio-Techne corporation, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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anti-ca9  (Servicebio Inc)
90
Servicebio Inc anti-ca9
Anti Ca9, supplied by Servicebio Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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anti-ca9 antibody  (ABclonal Biotechnology)
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ABclonal Biotechnology anti-ca9 antibody
Anti Ca9 Antibody, supplied by ABclonal Biotechnology, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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rabbit anti-ca9  (Cloud-Clone corp)
90
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A. Biochemical fractionation of normoxic/hypoxic U2OS cells into nuclear/ cytoplasmic fractions. The quality of fractionation was assessed by immunoblotting of nuclear marker Lamin A/C) and cytoplasmic marker (GAPDH). <t>CA9</t> immunoblotting was done to confirm the hypoxic condition. B. Realtime PCR of cCE-targeted lncRNAs shows differential expression patterns in cytoplasmic extracts of hypoxic osteosarcoma cells. Hypoxia significantly elevates the expression of ASH1L-AS1, GAS5-215, ENTPD3-AS1, and H19-009, whereas NFYC-AS1 expression was significantly reduced. Two-tail Student’s t-test was performed for statistical analysis C. Schematic representation of the dominant negative form of cytoplasmic restricted capping inhibited construct (K294A) BIO and myc denote the biotinylation and myc tag respectively. The nuclear export sequence (NES) of HIV is inserted in the N-terminus and the Nuclear Localization Signal (NLS) from the C-terminus is deleted to restrict expression of CE only in the cytoplasm. The lysine (K) residue at 294 th position is mutated to alanine (A) to make the construct catalytically inactive. D. Immunofluorescence image of myc tagged K294A expressing cells. E. Nuclear and cytoplasmic fractionation of U2OS cells expressing ±K294A under normoxic/hypoxic conditions. Western blot of Myc and CA9 confirm K294A expression and hypoxic condition respectively. The quality of fractionation was further substantiated by western blotting of nuclear marker (Lamin A/C) and cytoplasmic marker (GAPDH). F. The steady- state expression of GAS5-215, NFYC-AS1, and H19-009 was significantly decreased in K294A- expressing cells under normoxia. But ASH1L-AS1 was significantly increased and ENTPD3-AS1 did not show any significant change. G. The steady State expression of lncRNAs in hypoxic K294A cells. Only H19-009 showed significantly reduced expression in hypoxic K294A cells. Statistical significance was calculated by Two-tail Student’s t-test. Values are represented as ± SD from three biological replicates. ns, P: non-significant, *P< 0.05, **P< 0.005, ***P< 0.0005, ****P< 0.0001; n≥3.
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anti-ca9 gtx128428  (GeneTex)
90
GeneTex anti-ca9 gtx128428
A. Biochemical fractionation of normoxic/hypoxic U2OS cells into nuclear/ cytoplasmic fractions. The quality of fractionation was assessed by immunoblotting of nuclear marker Lamin A/C) and cytoplasmic marker (GAPDH). <t>CA9</t> immunoblotting was done to confirm the hypoxic condition. B. Realtime PCR of cCE-targeted lncRNAs shows differential expression patterns in cytoplasmic extracts of hypoxic osteosarcoma cells. Hypoxia significantly elevates the expression of ASH1L-AS1, GAS5-215, ENTPD3-AS1, and H19-009, whereas NFYC-AS1 expression was significantly reduced. Two-tail Student’s t-test was performed for statistical analysis C. Schematic representation of the dominant negative form of cytoplasmic restricted capping inhibited construct (K294A) BIO and myc denote the biotinylation and myc tag respectively. The nuclear export sequence (NES) of HIV is inserted in the N-terminus and the Nuclear Localization Signal (NLS) from the C-terminus is deleted to restrict expression of CE only in the cytoplasm. The lysine (K) residue at 294 th position is mutated to alanine (A) to make the construct catalytically inactive. D. Immunofluorescence image of myc tagged K294A expressing cells. E. Nuclear and cytoplasmic fractionation of U2OS cells expressing ±K294A under normoxic/hypoxic conditions. Western blot of Myc and CA9 confirm K294A expression and hypoxic condition respectively. The quality of fractionation was further substantiated by western blotting of nuclear marker (Lamin A/C) and cytoplasmic marker (GAPDH). F. The steady- state expression of GAS5-215, NFYC-AS1, and H19-009 was significantly decreased in K294A- expressing cells under normoxia. But ASH1L-AS1 was significantly increased and ENTPD3-AS1 did not show any significant change. G. The steady State expression of lncRNAs in hypoxic K294A cells. Only H19-009 showed significantly reduced expression in hypoxic K294A cells. Statistical significance was calculated by Two-tail Student’s t-test. Values are represented as ± SD from three biological replicates. ns, P: non-significant, *P< 0.05, **P< 0.005, ***P< 0.0005, ****P< 0.0001; n≥3.
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anti-ca9-antibodype mbs603443  (MyBiosource Biotechnology)
90
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A. Biochemical fractionation of normoxic/hypoxic U2OS cells into nuclear/ cytoplasmic fractions. The quality of fractionation was assessed by immunoblotting of nuclear marker Lamin A/C) and cytoplasmic marker (GAPDH). <t>CA9</t> immunoblotting was done to confirm the hypoxic condition. B. Realtime PCR of cCE-targeted lncRNAs shows differential expression patterns in cytoplasmic extracts of hypoxic osteosarcoma cells. Hypoxia significantly elevates the expression of ASH1L-AS1, GAS5-215, ENTPD3-AS1, and H19-009, whereas NFYC-AS1 expression was significantly reduced. Two-tail Student’s t-test was performed for statistical analysis C. Schematic representation of the dominant negative form of cytoplasmic restricted capping inhibited construct (K294A) BIO and myc denote the biotinylation and myc tag respectively. The nuclear export sequence (NES) of HIV is inserted in the N-terminus and the Nuclear Localization Signal (NLS) from the C-terminus is deleted to restrict expression of CE only in the cytoplasm. The lysine (K) residue at 294 th position is mutated to alanine (A) to make the construct catalytically inactive. D. Immunofluorescence image of myc tagged K294A expressing cells. E. Nuclear and cytoplasmic fractionation of U2OS cells expressing ±K294A under normoxic/hypoxic conditions. Western blot of Myc and CA9 confirm K294A expression and hypoxic condition respectively. The quality of fractionation was further substantiated by western blotting of nuclear marker (Lamin A/C) and cytoplasmic marker (GAPDH). F. The steady- state expression of GAS5-215, NFYC-AS1, and H19-009 was significantly decreased in K294A- expressing cells under normoxia. But ASH1L-AS1 was significantly increased and ENTPD3-AS1 did not show any significant change. G. The steady State expression of lncRNAs in hypoxic K294A cells. Only H19-009 showed significantly reduced expression in hypoxic K294A cells. Statistical significance was calculated by Two-tail Student’s t-test. Values are represented as ± SD from three biological replicates. ns, P: non-significant, *P< 0.05, **P< 0.005, ***P< 0.0005, ****P< 0.0001; n≥3.
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anti-ca9 2330  (Strategic Diagnostics Inc)
90
Strategic Diagnostics Inc anti-ca9 2330
A. Biochemical fractionation of normoxic/hypoxic U2OS cells into nuclear/ cytoplasmic fractions. The quality of fractionation was assessed by immunoblotting of nuclear marker Lamin A/C) and cytoplasmic marker (GAPDH). <t>CA9</t> immunoblotting was done to confirm the hypoxic condition. B. Realtime PCR of cCE-targeted lncRNAs shows differential expression patterns in cytoplasmic extracts of hypoxic osteosarcoma cells. Hypoxia significantly elevates the expression of ASH1L-AS1, GAS5-215, ENTPD3-AS1, and H19-009, whereas NFYC-AS1 expression was significantly reduced. Two-tail Student’s t-test was performed for statistical analysis C. Schematic representation of the dominant negative form of cytoplasmic restricted capping inhibited construct (K294A) BIO and myc denote the biotinylation and myc tag respectively. The nuclear export sequence (NES) of HIV is inserted in the N-terminus and the Nuclear Localization Signal (NLS) from the C-terminus is deleted to restrict expression of CE only in the cytoplasm. The lysine (K) residue at 294 th position is mutated to alanine (A) to make the construct catalytically inactive. D. Immunofluorescence image of myc tagged K294A expressing cells. E. Nuclear and cytoplasmic fractionation of U2OS cells expressing ±K294A under normoxic/hypoxic conditions. Western blot of Myc and CA9 confirm K294A expression and hypoxic condition respectively. The quality of fractionation was further substantiated by western blotting of nuclear marker (Lamin A/C) and cytoplasmic marker (GAPDH). F. The steady- state expression of GAS5-215, NFYC-AS1, and H19-009 was significantly decreased in K294A- expressing cells under normoxia. But ASH1L-AS1 was significantly increased and ENTPD3-AS1 did not show any significant change. G. The steady State expression of lncRNAs in hypoxic K294A cells. Only H19-009 showed significantly reduced expression in hypoxic K294A cells. Statistical significance was calculated by Two-tail Student’s t-test. Values are represented as ± SD from three biological replicates. ns, P: non-significant, *P< 0.05, **P< 0.005, ***P< 0.0005, ****P< 0.0001; n≥3.
Anti Ca9 2330, supplied by Strategic Diagnostics Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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anti-ca9, anti-pimonidazole polyclonal rabbit antibody  (Radboud University)
90
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A. Biochemical fractionation of normoxic/hypoxic U2OS cells into nuclear/ cytoplasmic fractions. The quality of fractionation was assessed by immunoblotting of nuclear marker Lamin A/C) and cytoplasmic marker (GAPDH). <t>CA9</t> immunoblotting was done to confirm the hypoxic condition. B. Realtime PCR of cCE-targeted lncRNAs shows differential expression patterns in cytoplasmic extracts of hypoxic osteosarcoma cells. Hypoxia significantly elevates the expression of ASH1L-AS1, GAS5-215, ENTPD3-AS1, and H19-009, whereas NFYC-AS1 expression was significantly reduced. Two-tail Student’s t-test was performed for statistical analysis C. Schematic representation of the dominant negative form of cytoplasmic restricted capping inhibited construct (K294A) BIO and myc denote the biotinylation and myc tag respectively. The nuclear export sequence (NES) of HIV is inserted in the N-terminus and the Nuclear Localization Signal (NLS) from the C-terminus is deleted to restrict expression of CE only in the cytoplasm. The lysine (K) residue at 294 th position is mutated to alanine (A) to make the construct catalytically inactive. D. Immunofluorescence image of myc tagged K294A expressing cells. E. Nuclear and cytoplasmic fractionation of U2OS cells expressing ±K294A under normoxic/hypoxic conditions. Western blot of Myc and CA9 confirm K294A expression and hypoxic condition respectively. The quality of fractionation was further substantiated by western blotting of nuclear marker (Lamin A/C) and cytoplasmic marker (GAPDH). F. The steady- state expression of GAS5-215, NFYC-AS1, and H19-009 was significantly decreased in K294A- expressing cells under normoxia. But ASH1L-AS1 was significantly increased and ENTPD3-AS1 did not show any significant change. G. The steady State expression of lncRNAs in hypoxic K294A cells. Only H19-009 showed significantly reduced expression in hypoxic K294A cells. Statistical significance was calculated by Two-tail Student’s t-test. Values are represented as ± SD from three biological replicates. ns, P: non-significant, *P< 0.05, **P< 0.005, ***P< 0.0005, ****P< 0.0001; n≥3.
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fully human parental anti-ca9 mab  (MorphoSys ag)
90
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A. Biochemical fractionation of normoxic/hypoxic U2OS cells into nuclear/ cytoplasmic fractions. The quality of fractionation was assessed by immunoblotting of nuclear marker Lamin A/C) and cytoplasmic marker (GAPDH). <t>CA9</t> immunoblotting was done to confirm the hypoxic condition. B. Realtime PCR of cCE-targeted lncRNAs shows differential expression patterns in cytoplasmic extracts of hypoxic osteosarcoma cells. Hypoxia significantly elevates the expression of ASH1L-AS1, GAS5-215, ENTPD3-AS1, and H19-009, whereas NFYC-AS1 expression was significantly reduced. Two-tail Student’s t-test was performed for statistical analysis C. Schematic representation of the dominant negative form of cytoplasmic restricted capping inhibited construct (K294A) BIO and myc denote the biotinylation and myc tag respectively. The nuclear export sequence (NES) of HIV is inserted in the N-terminus and the Nuclear Localization Signal (NLS) from the C-terminus is deleted to restrict expression of CE only in the cytoplasm. The lysine (K) residue at 294 th position is mutated to alanine (A) to make the construct catalytically inactive. D. Immunofluorescence image of myc tagged K294A expressing cells. E. Nuclear and cytoplasmic fractionation of U2OS cells expressing ±K294A under normoxic/hypoxic conditions. Western blot of Myc and CA9 confirm K294A expression and hypoxic condition respectively. The quality of fractionation was further substantiated by western blotting of nuclear marker (Lamin A/C) and cytoplasmic marker (GAPDH). F. The steady- state expression of GAS5-215, NFYC-AS1, and H19-009 was significantly decreased in K294A- expressing cells under normoxia. But ASH1L-AS1 was significantly increased and ENTPD3-AS1 did not show any significant change. G. The steady State expression of lncRNAs in hypoxic K294A cells. Only H19-009 showed significantly reduced expression in hypoxic K294A cells. Statistical significance was calculated by Two-tail Student’s t-test. Values are represented as ± SD from three biological replicates. ns, P: non-significant, *P< 0.05, **P< 0.005, ***P< 0.0005, ****P< 0.0001; n≥3.
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ca9 antibodies  (Boster Bio)
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A. Biochemical fractionation of normoxic/hypoxic U2OS cells into nuclear/ cytoplasmic fractions. The quality of fractionation was assessed by immunoblotting of nuclear marker Lamin A/C) and cytoplasmic marker (GAPDH). <t>CA9</t> immunoblotting was done to confirm the hypoxic condition. B. Realtime PCR of cCE-targeted lncRNAs shows differential expression patterns in cytoplasmic extracts of hypoxic osteosarcoma cells. Hypoxia significantly elevates the expression of ASH1L-AS1, GAS5-215, ENTPD3-AS1, and H19-009, whereas NFYC-AS1 expression was significantly reduced. Two-tail Student’s t-test was performed for statistical analysis C. Schematic representation of the dominant negative form of cytoplasmic restricted capping inhibited construct (K294A) BIO and myc denote the biotinylation and myc tag respectively. The nuclear export sequence (NES) of HIV is inserted in the N-terminus and the Nuclear Localization Signal (NLS) from the C-terminus is deleted to restrict expression of CE only in the cytoplasm. The lysine (K) residue at 294 th position is mutated to alanine (A) to make the construct catalytically inactive. D. Immunofluorescence image of myc tagged K294A expressing cells. E. Nuclear and cytoplasmic fractionation of U2OS cells expressing ±K294A under normoxic/hypoxic conditions. Western blot of Myc and CA9 confirm K294A expression and hypoxic condition respectively. The quality of fractionation was further substantiated by western blotting of nuclear marker (Lamin A/C) and cytoplasmic marker (GAPDH). F. The steady- state expression of GAS5-215, NFYC-AS1, and H19-009 was significantly decreased in K294A- expressing cells under normoxia. But ASH1L-AS1 was significantly increased and ENTPD3-AS1 did not show any significant change. G. The steady State expression of lncRNAs in hypoxic K294A cells. Only H19-009 showed significantly reduced expression in hypoxic K294A cells. Statistical significance was calculated by Two-tail Student’s t-test. Values are represented as ± SD from three biological replicates. ns, P: non-significant, *P< 0.05, **P< 0.005, ***P< 0.0005, ****P< 0.0001; n≥3.
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carbonic anhydrase ix/ca9 antibody (2d3) - bsa free  (Bio-Techne corporation)
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A. Biochemical fractionation of normoxic/hypoxic U2OS cells into nuclear/ cytoplasmic fractions. The quality of fractionation was assessed by immunoblotting of nuclear marker Lamin A/C) and cytoplasmic marker (GAPDH). <t>CA9</t> immunoblotting was done to confirm the hypoxic condition. B. Realtime PCR of cCE-targeted lncRNAs shows differential expression patterns in cytoplasmic extracts of hypoxic osteosarcoma cells. Hypoxia significantly elevates the expression of ASH1L-AS1, GAS5-215, ENTPD3-AS1, and H19-009, whereas NFYC-AS1 expression was significantly reduced. Two-tail Student’s t-test was performed for statistical analysis C. Schematic representation of the dominant negative form of cytoplasmic restricted capping inhibited construct (K294A) BIO and myc denote the biotinylation and myc tag respectively. The nuclear export sequence (NES) of HIV is inserted in the N-terminus and the Nuclear Localization Signal (NLS) from the C-terminus is deleted to restrict expression of CE only in the cytoplasm. The lysine (K) residue at 294 th position is mutated to alanine (A) to make the construct catalytically inactive. D. Immunofluorescence image of myc tagged K294A expressing cells. E. Nuclear and cytoplasmic fractionation of U2OS cells expressing ±K294A under normoxic/hypoxic conditions. Western blot of Myc and CA9 confirm K294A expression and hypoxic condition respectively. The quality of fractionation was further substantiated by western blotting of nuclear marker (Lamin A/C) and cytoplasmic marker (GAPDH). F. The steady- state expression of GAS5-215, NFYC-AS1, and H19-009 was significantly decreased in K294A- expressing cells under normoxia. But ASH1L-AS1 was significantly increased and ENTPD3-AS1 did not show any significant change. G. The steady State expression of lncRNAs in hypoxic K294A cells. Only H19-009 showed significantly reduced expression in hypoxic K294A cells. Statistical significance was calculated by Two-tail Student’s t-test. Values are represented as ± SD from three biological replicates. ns, P: non-significant, *P< 0.05, **P< 0.005, ***P< 0.0005, ****P< 0.0001; n≥3.
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mouse carbonic anhydrase ix/ca9 antibody  (Bio-Techne corporation)
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A. Biochemical fractionation of normoxic/hypoxic U2OS cells into nuclear/ cytoplasmic fractions. The quality of fractionation was assessed by immunoblotting of nuclear marker Lamin A/C) and cytoplasmic marker (GAPDH). <t>CA9</t> immunoblotting was done to confirm the hypoxic condition. B. Realtime PCR of cCE-targeted lncRNAs shows differential expression patterns in cytoplasmic extracts of hypoxic osteosarcoma cells. Hypoxia significantly elevates the expression of ASH1L-AS1, GAS5-215, ENTPD3-AS1, and H19-009, whereas NFYC-AS1 expression was significantly reduced. Two-tail Student’s t-test was performed for statistical analysis C. Schematic representation of the dominant negative form of cytoplasmic restricted capping inhibited construct (K294A) BIO and myc denote the biotinylation and myc tag respectively. The nuclear export sequence (NES) of HIV is inserted in the N-terminus and the Nuclear Localization Signal (NLS) from the C-terminus is deleted to restrict expression of CE only in the cytoplasm. The lysine (K) residue at 294 th position is mutated to alanine (A) to make the construct catalytically inactive. D. Immunofluorescence image of myc tagged K294A expressing cells. E. Nuclear and cytoplasmic fractionation of U2OS cells expressing ±K294A under normoxic/hypoxic conditions. Western blot of Myc and CA9 confirm K294A expression and hypoxic condition respectively. The quality of fractionation was further substantiated by western blotting of nuclear marker (Lamin A/C) and cytoplasmic marker (GAPDH). F. The steady- state expression of GAS5-215, NFYC-AS1, and H19-009 was significantly decreased in K294A- expressing cells under normoxia. But ASH1L-AS1 was significantly increased and ENTPD3-AS1 did not show any significant change. G. The steady State expression of lncRNAs in hypoxic K294A cells. Only H19-009 showed significantly reduced expression in hypoxic K294A cells. Statistical significance was calculated by Two-tail Student’s t-test. Values are represented as ± SD from three biological replicates. ns, P: non-significant, *P< 0.05, **P< 0.005, ***P< 0.0005, ****P< 0.0001; n≥3.
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Image Search Results


A. Biochemical fractionation of normoxic/hypoxic U2OS cells into nuclear/ cytoplasmic fractions. The quality of fractionation was assessed by immunoblotting of nuclear marker Lamin A/C) and cytoplasmic marker (GAPDH). CA9 immunoblotting was done to confirm the hypoxic condition. B. Realtime PCR of cCE-targeted lncRNAs shows differential expression patterns in cytoplasmic extracts of hypoxic osteosarcoma cells. Hypoxia significantly elevates the expression of ASH1L-AS1, GAS5-215, ENTPD3-AS1, and H19-009, whereas NFYC-AS1 expression was significantly reduced. Two-tail Student’s t-test was performed for statistical analysis C. Schematic representation of the dominant negative form of cytoplasmic restricted capping inhibited construct (K294A) BIO and myc denote the biotinylation and myc tag respectively. The nuclear export sequence (NES) of HIV is inserted in the N-terminus and the Nuclear Localization Signal (NLS) from the C-terminus is deleted to restrict expression of CE only in the cytoplasm. The lysine (K) residue at 294 th position is mutated to alanine (A) to make the construct catalytically inactive. D. Immunofluorescence image of myc tagged K294A expressing cells. E. Nuclear and cytoplasmic fractionation of U2OS cells expressing ±K294A under normoxic/hypoxic conditions. Western blot of Myc and CA9 confirm K294A expression and hypoxic condition respectively. The quality of fractionation was further substantiated by western blotting of nuclear marker (Lamin A/C) and cytoplasmic marker (GAPDH). F. The steady- state expression of GAS5-215, NFYC-AS1, and H19-009 was significantly decreased in K294A- expressing cells under normoxia. But ASH1L-AS1 was significantly increased and ENTPD3-AS1 did not show any significant change. G. The steady State expression of lncRNAs in hypoxic K294A cells. Only H19-009 showed significantly reduced expression in hypoxic K294A cells. Statistical significance was calculated by Two-tail Student’s t-test. Values are represented as ± SD from three biological replicates. ns, P: non-significant, *P< 0.05, **P< 0.005, ***P< 0.0005, ****P< 0.0001; n≥3.

Journal: bioRxiv

Article Title: Cytoplasmic mRNA capping enzyme is regulated by hypoxia inducible factor HIF1α and controls stability of the target lncRNAs during hypoxia

doi: 10.1101/2024.07.05.602207

Figure Lengend Snippet: A. Biochemical fractionation of normoxic/hypoxic U2OS cells into nuclear/ cytoplasmic fractions. The quality of fractionation was assessed by immunoblotting of nuclear marker Lamin A/C) and cytoplasmic marker (GAPDH). CA9 immunoblotting was done to confirm the hypoxic condition. B. Realtime PCR of cCE-targeted lncRNAs shows differential expression patterns in cytoplasmic extracts of hypoxic osteosarcoma cells. Hypoxia significantly elevates the expression of ASH1L-AS1, GAS5-215, ENTPD3-AS1, and H19-009, whereas NFYC-AS1 expression was significantly reduced. Two-tail Student’s t-test was performed for statistical analysis C. Schematic representation of the dominant negative form of cytoplasmic restricted capping inhibited construct (K294A) BIO and myc denote the biotinylation and myc tag respectively. The nuclear export sequence (NES) of HIV is inserted in the N-terminus and the Nuclear Localization Signal (NLS) from the C-terminus is deleted to restrict expression of CE only in the cytoplasm. The lysine (K) residue at 294 th position is mutated to alanine (A) to make the construct catalytically inactive. D. Immunofluorescence image of myc tagged K294A expressing cells. E. Nuclear and cytoplasmic fractionation of U2OS cells expressing ±K294A under normoxic/hypoxic conditions. Western blot of Myc and CA9 confirm K294A expression and hypoxic condition respectively. The quality of fractionation was further substantiated by western blotting of nuclear marker (Lamin A/C) and cytoplasmic marker (GAPDH). F. The steady- state expression of GAS5-215, NFYC-AS1, and H19-009 was significantly decreased in K294A- expressing cells under normoxia. But ASH1L-AS1 was significantly increased and ENTPD3-AS1 did not show any significant change. G. The steady State expression of lncRNAs in hypoxic K294A cells. Only H19-009 showed significantly reduced expression in hypoxic K294A cells. Statistical significance was calculated by Two-tail Student’s t-test. Values are represented as ± SD from three biological replicates. ns, P: non-significant, *P< 0.05, **P< 0.005, ***P< 0.0005, ****P< 0.0001; n≥3.

Article Snippet: The dilutions are as follows 1:1000 rabbit anti-HIF1α (Cell Signaling Technology, cat no# 36169); 1:1000 mouse anti-beta actin (Santa Cruz Biotechnology, cat no# SC-517582), 1:1000 rabbit anti-CE (Novus Biologicals, cat no# NPB1- 49973); 1:2000 mouse anti-GAPDH (Novus Biologicals, cat#2D4A7); 1:2000 rabbit anti-CA9 (Cloud-clone Crop, cat# PAD076Hu01); 1:2000 mouse anti-lamin (DHSB, cat no# MANLAC1(4A7) at 4 JC overnight with constant shaking.

Techniques: Fractionation, Western Blot, Marker, Expressing, Dominant Negative Mutation, Construct, Sequencing, Residue, Immunofluorescence