Journal: Bioconjugate Chemistry

Article Title: Enhancing Reactivity for Bioorthogonal Pretargeting by Unmasking Antibody-Conjugated trans-Cyclooctenes

doi: 10.1021/bc500605g

Figure Lengend Snippet: Figure 5. Translation of dual orthogonal coupling to different antibodies. (A) Anti-TfR and EpCAM antibodies had low TCO reactivity (12−15%) after direct amine-conjugation, but the PEG24− TCO increased reactive TCO density 3- to 4-fold. *MALDI-TOF analysis of the TCO−PEG24-modified anti-EpCAM antibody was limited by difficulties with desorption. (B) Flow cytometry results for TCO- and TCO−PEG24-modified anti-EpCAM and TfR antibodies on A431 cells after reaction with 10 nM tetrazine−QDs for 30 min, showing 5-fold signal enhancements for the PEG24−TCO. (C) Confocal images of A431 cells labeled with (i, iv) anti-EpCAM, (ii, v) anti-TfR, or (iii, vi) nonbinding control antibodies. The antibodies were modified with (i−iii) TCO or (iv−vi) PEG24−TCO at the highest densities and reacted with tetrazine−QD at 10 nM for 30 min. Scale bar: 20 μm. Error bars represent the standard error of at least three independent experiments.

Article Snippet: Monoclonal mouse antibodies specific for human EpCAM (IgG2b, clone 158206) and transferrin receptor (TfR, IgG1, clone 29806) were purchased from R&D Systems (Minneapolis, MN).

Techniques: Conjugation Assay, Flow Cytometry, Labeling, Control

Journal:

Article Title: Phospholipase D2 Is Localized to the Rims of the Golgi Apparatus in Mammalian Cells

doi: 10.1091/mbc.02-04-0059

Figure Lengend Snippet: PLD2 localization with different organelles in rat NRK cells. Cells were prepared for immunofluorescence microscopy and incubated with rabbit PLD2 antibody PLD2-27 (A, D, G, and J) and costained with monoclonal antibodies to the ER marker BiP (B); the transferrin receptor (E), a marker of early endosomes and the plasma membrane; and lgp120 (H), a late endosome/lysosomal marker. (K) Cells were stained with caveolin-1, a marker for caveoli; the arrow corresponds to caveolin-1 localized to the plasma membrane. Images were merged to determine overlap between PLD2 (red) and the respective marker proteins (green; C, F, I, and L). Areas of maximal overlap are yellow. (L) Inset, enlargement of the region of overlap between PLD2 and caveolin-1 in the perinuclear Golgi apparatus. The arrow indicates areas where PLD2 puncta (red) either overlap completely with caveolin-1 (green) or are directly adjacent. Images are from projected Z-series. Bars, 10 μm.

Article Snippet: Purified monoclonal anti-rat transferrin receptor antibodies were purchased from Cedarlane Laboratories (Hornby, Ontario, Canada).

Techniques: Immunofluorescence, Microscopy, Incubation, Marker, Staining

Journal: Nutrients

Article Title: Construction and Evaluation of a Novel MAP Immunoassay for 9 Nutrition-and-Health-Related Protein Markers Based on Multiplex Liquid Protein Chip Technique

doi: 10.3390/nu15061522

Figure Lengend Snippet: Reagents used in this research.

Article Snippet: , sTfR detection antiboty , NB100-73092B , Novus Biologicals, Inc. (Centennial, CO, USA).

Techniques: Comparison, Enzyme-linked Immunosorbent Assay, Diagnostic Assay

Journal: Nutrients

Article Title: Construction and Evaluation of a Novel MAP Immunoassay for 9 Nutrition-and-Health-Related Protein Markers Based on Multiplex Liquid Protein Chip Technique

doi: 10.3390/nu15061522

Figure Lengend Snippet: The cross-reaction rates of 9 protein markers with each detection antibody (%).

Article Snippet: , sTfR detection antiboty , NB100-73092B , Novus Biologicals, Inc. (Centennial, CO, USA).

Techniques:

Journal: Nutrients

Article Title: Construction and Evaluation of a Novel MAP Immunoassay for 9 Nutrition-and-Health-Related Protein Markers Based on Multiplex Liquid Protein Chip Technique

doi: 10.3390/nu15061522

Figure Lengend Snippet: The cross-reaction rates of 9 protein markers with each capture antibody (%).

Article Snippet: , sTfR detection antiboty , NB100-73092B , Novus Biologicals, Inc. (Centennial, CO, USA).

Techniques:

Journal: Nutrients

Article Title: Construction and Evaluation of a Novel MAP Immunoassay for 9 Nutrition-and-Health-Related Protein Markers Based on Multiplex Liquid Protein Chip Technique

doi: 10.3390/nu15061522

Figure Lengend Snippet: The LLDs and BDLs of liquid protein chip for 9 protein markers (pg/mL).

Article Snippet: , sTfR detection antiboty , NB100-73092B , Novus Biologicals, Inc. (Centennial, CO, USA).

Techniques:

Journal: Nutrients

Article Title: Construction and Evaluation of a Novel MAP Immunoassay for 9 Nutrition-and-Health-Related Protein Markers Based on Multiplex Liquid Protein Chip Technique

doi: 10.3390/nu15061522

Figure Lengend Snippet: The S -curves of 9 protein markers in the same one detection by using liquid protein chip. ( A ): SF S -curve; ( B ): sTfR S -curve; ( C ): CRP S -curve; ( D ): RBP4 S -curve; ( E ): ApoB S -curve; ( F ): AFP S -curve; ( G ): PA S -curve; ( H ): CEA S -curve; ( I ): D-D S -curve.

Article Snippet: , sTfR detection antiboty , NB100-73092B , Novus Biologicals, Inc. (Centennial, CO, USA).

Techniques:

Journal: Nutrients

Article Title: Construction and Evaluation of a Novel MAP Immunoassay for 9 Nutrition-and-Health-Related Protein Markers Based on Multiplex Liquid Protein Chip Technique

doi: 10.3390/nu15061522

Figure Lengend Snippet: The regression equations and determination coefficients of 9 protein markers detected by the liquid protein chip.

Article Snippet: , sTfR detection antiboty , NB100-73092B , Novus Biologicals, Inc. (Centennial, CO, USA).

Techniques: Marker

Journal: Nutrients

Article Title: Construction and Evaluation of a Novel MAP Immunoassay for 9 Nutrition-and-Health-Related Protein Markers Based on Multiplex Liquid Protein Chip Technique

doi: 10.3390/nu15061522

Figure Lengend Snippet: The recovery rates of 9 protein markers detected by liquid protein chip simultaneously ( n = 3).

Article Snippet: , sTfR detection antiboty , NB100-73092B , Novus Biologicals, Inc. (Centennial, CO, USA).

Techniques:

Journal: Nutrients

Article Title: Construction and Evaluation of a Novel MAP Immunoassay for 9 Nutrition-and-Health-Related Protein Markers Based on Multiplex Liquid Protein Chip Technique

doi: 10.3390/nu15061522

Figure Lengend Snippet: The within-run precisions of 9 protein markers by liquid protein chip ( n = 10).

Article Snippet: , sTfR detection antiboty , NB100-73092B , Novus Biologicals, Inc. (Centennial, CO, USA).

Techniques: Chromatin Immunoprecipitation

Journal: Nutrients

Article Title: Construction and Evaluation of a Novel MAP Immunoassay for 9 Nutrition-and-Health-Related Protein Markers Based on Multiplex Liquid Protein Chip Technique

doi: 10.3390/nu15061522

Figure Lengend Snippet: The between-run precisions of 9 protein markers by liquid protein chip ( D = 6, n = 3).

Article Snippet: , sTfR detection antiboty , NB100-73092B , Novus Biologicals, Inc. (Centennial, CO, USA).

Techniques: Chromatin Immunoprecipitation

Journal: Nutrients

Article Title: Construction and Evaluation of a Novel MAP Immunoassay for 9 Nutrition-and-Health-Related Protein Markers Based on Multiplex Liquid Protein Chip Technique

doi: 10.3390/nu15061522

Figure Lengend Snippet: Correlation analysis between liquid protein chip method and other methods for detecting the 9 protein markers of the same 16 serum samples. ( A ): The correlation analysis of SF; ( B ): The correlation analysis of sTfR; ( C ): The correlation analysis of CRP; ( D ): The correlation analysis of RBP4; ( E ): The correlation analysis of ApoB; ( F ): The correlation analysis of AFP; ( G ): The correlation analysis of PA; ( H ): The correlation analysis of CEA; ( I ): The correlation analysis of D-D.

Article Snippet: , sTfR detection antiboty , NB100-73092B , Novus Biologicals, Inc. (Centennial, CO, USA).

Techniques:

Journal: Nutrients

Article Title: Construction and Evaluation of a Novel MAP Immunoassay for 9 Nutrition-and-Health-Related Protein Markers Based on Multiplex Liquid Protein Chip Technique

doi: 10.3390/nu15061522

Figure Lengend Snippet: The correlation analysis between the liquid protein chip method and other methods for the detection of 9 protein markers in the same 16 serums ( n = 16).

Article Snippet: , sTfR detection antiboty , NB100-73092B , Novus Biologicals, Inc. (Centennial, CO, USA).

Techniques: Enzyme-linked Immunosorbent Assay

Journal: Nutrients

Article Title: Construction and Evaluation of a Novel MAP Immunoassay for 9 Nutrition-and-Health-Related Protein Markers Based on Multiplex Liquid Protein Chip Technique

doi: 10.3390/nu15061522

Figure Lengend Snippet: The paired comparison between liquid protein chip method and other methods for the detection of 9 protein markers of serums ( n = 16).

Article Snippet: , sTfR detection antiboty , NB100-73092B , Novus Biologicals, Inc. (Centennial, CO, USA).

Techniques: Comparison, Enzyme-linked Immunosorbent Assay

Journal: Nutrients

Article Title: Construction and Evaluation of a Novel MAP Immunoassay for 9 Nutrition-and-Health-Related Protein Markers Based on Multiplex Liquid Protein Chip Technique

doi: 10.3390/nu15061522

Figure Lengend Snippet: The analytical specificities of different interferences for the liquid protein chip in detecting 9 protein markers (%).

Article Snippet: , sTfR detection antiboty , NB100-73092B , Novus Biologicals, Inc. (Centennial, CO, USA).

Techniques:

Journal: Nutrients

Article Title: Construction and Evaluation of a Novel MAP Immunoassay for 9 Nutrition-and-Health-Related Protein Markers Based on Multiplex Liquid Protein Chip Technique

doi: 10.3390/nu15061522

Figure Lengend Snippet: The comparison of the LLDs of 9 protein markers between liquid protein chip and other methods.

Article Snippet: , sTfR detection antiboty , NB100-73092B , Novus Biologicals, Inc. (Centennial, CO, USA).

Techniques: Comparison, Enzyme-linked Immunosorbent Assay

Journal: Pediatric Research

Article Title: Plasma Biomarkers in Pediatric Patients Undergoing Cardiopulmonary Bypass

doi: 10.1203/pdr.0b013e31816e391f

Figure Lengend Snippet: Figure 2. Effect of pediatric CPB procedures on plasma iron, copper, and related proteins. Measurements were made from samples taken at five time points before, during, and after surgery in related metals and proteins: (A) ceruloplasmin and copper, (B) transferrin and iron, (C) hemopexin and Hb, and (D) hematocrit. The insets show representative western blot signals for ceruloplasmin, transferrin, and hemopexin. *p 0.05; **p 0.01.

Article Snippet: Blots were then probed with antibodies for inter-alpha inhibitor H4 (ITI-H4; Santa Cruz Biotechnology, Inc.), ceruloplasmin (Novus Biologicals), hemopexin (Novus Biologicals), transferrin (Novus Biologicals) and alpha-2-macroglobulin (Sigma Chemical Co.Aldrich).

Techniques: Clinical Proteomics, Western Blot