Journal: Inflammatory Bowel Diseases

Article Title: Infliximab Restores the Dysfunctional Matrix Remodeling Protein and Growth Factor Gene Expression in Patients with Inflammatory Bowel Disease

doi: 10.1097/01.mib.0000438430.15553.90

Figure Lengend Snippet: FIGURE 1. qRT-PCR analysis of MMP1 (A), MMP28 (B), TIMP1 (C), ADAM9 (D), TFF1 (E), and TGFB1 (F) in intestinal mucosa from patients with IBD before and after the first infliximab treatment and controls. A line between 2 points represents the change in expression before and after treatment for 1 patient. The x axis labels of subfigures 1A, 1B, 1C, and 1D are similar as shown in subfigure 1E and 1F. NR, infliximab non- responders; R, infliximab responders. *Significant P #0.01.

Article Snippet: After a second wash step, sections were incubated with an anti–human MMP1 rabbit polyclonal antibody (Bio-Rad AbD Serotec GmbH, Düsseldorf, Germany; dilution 1/100), or with an anti–human MMP3 rabbit polyclonal antibody (Sigma-Aldrich, dilution 1/200), or with anti–human TIMP1 mouse monoclonal antibody (clone 102D1; Millipore, Overijse, Belgium; dilution 1/75), or with anti–human REG1A rabbit polyclonal antibody (Sigma-Aldrich, dilution 1/300) for 30 minutes at room temperature.

Techniques: Quantitative RT-PCR, Expressing

Journal: Inflammatory Bowel Diseases

Article Title: Infliximab Restores the Dysfunctional Matrix Remodeling Protein and Growth Factor Gene Expression in Patients with Inflammatory Bowel Disease

doi: 10.1097/01.mib.0000438430.15553.90

Figure Lengend Snippet: FIGURE 2. The ratio MMP1/TIMP1 in intestinal mucosa from patients with IBD before and after the first infliximab treatment and controls, using the qRT-PCR expression data of MMP1 and TIMP1. A line between 2 points represents the change in expression before and after treatment for 1 patient. NR, infliximab nonresponders; R, infliximab responders. *Significant P #0.01.

Article Snippet: After a second wash step, sections were incubated with an anti–human MMP1 rabbit polyclonal antibody (Bio-Rad AbD Serotec GmbH, Düsseldorf, Germany; dilution 1/100), or with an anti–human MMP3 rabbit polyclonal antibody (Sigma-Aldrich, dilution 1/200), or with anti–human TIMP1 mouse monoclonal antibody (clone 102D1; Millipore, Overijse, Belgium; dilution 1/75), or with anti–human REG1A rabbit polyclonal antibody (Sigma-Aldrich, dilution 1/300) for 30 minutes at room temperature.

Techniques: Quantitative RT-PCR, Expressing

Journal: Inflammatory Bowel Diseases

Article Title: Infliximab Restores the Dysfunctional Matrix Remodeling Protein and Growth Factor Gene Expression in Patients with Inflammatory Bowel Disease

doi: 10.1097/01.mib.0000438430.15553.90

Figure Lengend Snippet: FIGURE 3. Ileal and colonic tissues from controls, patients with active CDi, and patients with active IBDc were stained by immunohistochemistry to localize MMP1 (A), MMP3 (B), REG1A (C), and TIMP1 (D). Arrowheads in the lower panel D indicate TIMP1 expression in enteroendocrine cells. Images were taken at a 20-fold magnification; scale bars represent 100 mm and are shown in the right lower corner.

Article Snippet: After a second wash step, sections were incubated with an anti–human MMP1 rabbit polyclonal antibody (Bio-Rad AbD Serotec GmbH, Düsseldorf, Germany; dilution 1/100), or with an anti–human MMP3 rabbit polyclonal antibody (Sigma-Aldrich, dilution 1/200), or with anti–human TIMP1 mouse monoclonal antibody (clone 102D1; Millipore, Overijse, Belgium; dilution 1/75), or with anti–human REG1A rabbit polyclonal antibody (Sigma-Aldrich, dilution 1/300) for 30 minutes at room temperature.

Techniques: Staining, Immunohistochemistry, Expressing

Journal: Journal of Oncology

Article Title: Identification of Prognostic Biomarkers of Glioblastoma Based on Multidatabase Integration and Its Correlation with Immune-Infiltration Cells

doi: 10.1155/2022/3909030

Figure Lengend Snippet: The common DEGs of four gene expression profiles (adj. P value <0.05, |logFC|> 2.0).

Article Snippet: After blocking with 5% fat-free milk, incubate with primary antibodies against TIMP1 (A00561-1; Boster, China) and β -actin (Beyotime Biotechnology, China), respectively, using a dilution of 1 : 1000 for each antibody overnight at 4°C.

Techniques: Gene Expression

Journal: Journal of Oncology

Article Title: Identification of Prognostic Biomarkers of Glioblastoma Based on Multidatabase Integration and Its Correlation with Immune-Infiltration Cells

doi: 10.1155/2022/3909030

Figure Lengend Snippet: PPI network and hub genes' identification. (a) PPI network was structured by the 54 upregulated DEGs using STRING database. (b) The top 10 hub genes in the PPI network were screened ground on their connectivity degree. The genes such as CD44, COL1A1, COL1A2, COL3A1, FN1, IGFBP3, LOX, POSTN, TIMP1, and VEGFA are represented from red (high degree) to yellow (low degree). (c) Gene Ontology (GO) chord diagram of the top genes in GBM.

Article Snippet: After blocking with 5% fat-free milk, incubate with primary antibodies against TIMP1 (A00561-1; Boster, China) and β -actin (Beyotime Biotechnology, China), respectively, using a dilution of 1 : 1000 for each antibody overnight at 4°C.

Techniques:

Journal: Journal of Oncology

Article Title: Identification of Prognostic Biomarkers of Glioblastoma Based on Multidatabase Integration and Its Correlation with Immune-Infiltration Cells

doi: 10.1155/2022/3909030

Figure Lengend Snippet: Top ten hub genes with higher degree of connectivity.

Article Snippet: After blocking with 5% fat-free milk, incubate with primary antibodies against TIMP1 (A00561-1; Boster, China) and β -actin (Beyotime Biotechnology, China), respectively, using a dilution of 1 : 1000 for each antibody overnight at 4°C.

Techniques: Binding Assay

Journal: Journal of Oncology

Article Title: Identification of Prognostic Biomarkers of Glioblastoma Based on Multidatabase Integration and Its Correlation with Immune-Infiltration Cells

doi: 10.1155/2022/3909030

Figure Lengend Snippet: The AUC analysis of hub genes. AUC analysis of CD44, COL1A1, COL1A2, COL3A1, FN1, IGFBP3, LOX, POSTN, TIMP1, and VEGFA for distinguishing GBM samples from normal tissues.

Article Snippet: After blocking with 5% fat-free milk, incubate with primary antibodies against TIMP1 (A00561-1; Boster, China) and β -actin (Beyotime Biotechnology, China), respectively, using a dilution of 1 : 1000 for each antibody overnight at 4°C.

Techniques:

Journal: Journal of Oncology

Article Title: Identification of Prognostic Biomarkers of Glioblastoma Based on Multidatabase Integration and Its Correlation with Immune-Infiltration Cells

doi: 10.1155/2022/3909030

Figure Lengend Snippet: OS, DFS, and univariate and multivariate Cox analysis of the hub genes overexpressed in GBM patients. (a) OS of the hub genes overexpressed in GBM sufferers. (b) DFS of the hub genes overexpressed in GBM sufferers. (c) Univariate and multivariate Cox analysis of TIMP1 expression and other clinical pathological factors for OS. HR, hazard ratio.

Article Snippet: After blocking with 5% fat-free milk, incubate with primary antibodies against TIMP1 (A00561-1; Boster, China) and β -actin (Beyotime Biotechnology, China), respectively, using a dilution of 1 : 1000 for each antibody overnight at 4°C.

Techniques: Expressing

Journal: Journal of Oncology

Article Title: Identification of Prognostic Biomarkers of Glioblastoma Based on Multidatabase Integration and Its Correlation with Immune-Infiltration Cells

doi: 10.1155/2022/3909030

Figure Lengend Snippet: Correlation between immune infiltrates and TIMP1 expression in GBM. (a) TIMP1 expression was definitely connected with CD4+ T cell, B cell, T cell regulatory (Tregs), neutrophils, cancer-associated fibroblast, macrophage M1, and myeloid dendritic cell. (b–d) Higher infiltration of Tregs, cancer-associated fibroblast, and mast cell correlated with worse prognosis.

Article Snippet: After blocking with 5% fat-free milk, incubate with primary antibodies against TIMP1 (A00561-1; Boster, China) and β -actin (Beyotime Biotechnology, China), respectively, using a dilution of 1 : 1000 for each antibody overnight at 4°C.

Techniques: Expressing

Journal: Journal of Oncology

Article Title: Identification of Prognostic Biomarkers of Glioblastoma Based on Multidatabase Integration and Its Correlation with Immune-Infiltration Cells

doi: 10.1155/2022/3909030

Figure Lengend Snippet: Correlation analysis between TIMP1 and related genes and markers of immune cells in TIMER.

Article Snippet: After blocking with 5% fat-free milk, incubate with primary antibodies against TIMP1 (A00561-1; Boster, China) and β -actin (Beyotime Biotechnology, China), respectively, using a dilution of 1 : 1000 for each antibody overnight at 4°C.

Techniques:

Journal: Journal of Oncology

Article Title: Identification of Prognostic Biomarkers of Glioblastoma Based on Multidatabase Integration and Its Correlation with Immune-Infiltration Cells

doi: 10.1155/2022/3909030

Figure Lengend Snippet: Downregulation of TIMP1 inhibits cell proliferation, migration, and invasion in U-87 MG and U-118 MG cells. (a, b) The results of qPCR and WB showed that compared with many glioblastoma cell lines, U-87 MG and U-118 MG cell lines were selected for TIMP1 gene related function test. (c, d) qPCR and WB showed that TIMP1 expression was inhibited in U-87 MG and U-118 MG cells transfected with sh-TIMP1 compared with the NC group. (e, f) CCK-8 and colony formation assays revealed that the proliferation abilities were suppressed in U-87 MG and U-118 MG cells transfected with sh-TIMP1 compared with the NC group. (g, h) Transwell assay revealed that cell migration and invasion were suppressed in U-87 MG and U-118 MG cells transfected with sh-TIMP1 compared with the NC group. ∗ P < 0.05; ∗∗ P < 0.01; ∗∗∗ P < 0.001.

Article Snippet: After blocking with 5% fat-free milk, incubate with primary antibodies against TIMP1 (A00561-1; Boster, China) and β -actin (Beyotime Biotechnology, China), respectively, using a dilution of 1 : 1000 for each antibody overnight at 4°C.

Techniques: Migration, Expressing, Transfection, CCK-8 Assay, Transwell Assay