Journal: Brain, behavior, and immunity

Article Title: Rats bred for low intrinsic aerobic exercise capacity link obesity with brain inflammation and reduced structural plasticity of the hippocampus.

doi: 10.1016/j.bbi.2021.06.017

Figure Lengend Snippet: Fig. 4. Older rats expressed less synaptic proteins (arbitrary units) in the hippocampus compared to young rats and the difference is more drastic in low-capacity Runner (LCR) rats than in high- capacity runner (HCR) rats. Western blot analyses of the expression of (A) synapsin (SYN-1) and (B) synaptophysin (SYP) in hippocampal homogenates of HCR and LCR rats at the age of 8 weeks (young) and 10 months (old). The membrane was cut horizontally to separate SYN-1 and SYP according to their molec ular weights, which are shown with the representative blot image. The graphs show the amount of the pro teins normalized to the total protein as analyzed from the stain-free blot. From the stain-free blot only the band of approximately 50 kDa is shown. The boxes and whiskers are the same as in Fig. 1. Mann-Whitney U test. * p ≤0.050, ** p ≤0.010 and *** p ≤0.001.

Article Snippet: Before that, the membranes were blocked for 1 h at RT with OdysseyR Blocking buffer (LI-COR 927–40000, Lincoln, NE, USA) and then incubated overnight at + 4 ◦C with primary antibodies against synaptic markers SYN-1 (ANR-014, Alomone labs, Jerusalem, Israel, 1:1000 dilution) and SYP (ANR-013, Alomone labs, Jerusalem, Israel, 1:1000), glial marker GFAP (ab7260, Abcam, Cambridge, UK, 1:5000 dilution) and microglial marker IBA1 (PA5-27436, ThermoFisher Scientific, Rockford, IL, USA, 1:700 dilution).

Techniques: Western Blot, Expressing, Membrane, Staining, MANN-WHITNEY

Journal: International Journal of Molecular Sciences

Article Title: Mesenchymal Stem Cells Restore Endothelial Integrity and Alleviate Emotional Impairments in a Diabetic Mouse Model via Inhibition of MMP-9 Activity

doi: 10.3390/ijms26073355

Figure Lengend Snippet: Figure 2. DM mice exhibit synaptic deficits and increased neuroinflammation in the HIP. (A) Repre- sentative WB images showing the expression levels of the presynaptic protein synapsin I (SYN1) and the postsynaptic protein postsynaptic density protein 95 (PSD95) in the HIP of CTL and DM mice. β-actin was used as an internal control. (B) Semi-quantitative analysis of PSD95 and SYN1 expression levels from immunoblot experiments. n = 6. (C) Representative images of Iba1 immunostaining in the HIP of CTL and DM mice. The area within the dashed box is magnified and displayed in the insets. Scale bars: 100 µm (main images) and 20 µm (insets). (D) Quantitative analysis of Iba1-positive microglia in the HIP of CTL and DM mice. n = 5. (E) qPCR analysis of iNOS and Arg1 mRNA levels in the HIP of CTL and DM mice. n = 6. (F) qPCR analysis of IL-6, IL-1β, and TNF-α mRNA levels in the HIP of CTL and DM mice. n = 6. (G) ELISA measuring IL-6, IL-1β, and TNF-α levels in the serum of CTL and DM mice. n = 3. Data are presented as mean ± SEM. Statistical significance was determined using a one-tailed unpaired t-test. * p < 0.05, ** p < 0.01, *** p < 0.001.

Article Snippet: The membranes were blocked and incubated overnight with the following antibodies: PSD95 (SYSN, Toronto, ON, Canada, 124002, 1:1000); SYN1 (SYSN, 106011, 1:1000); Cldn5 (Invitrogen, 35-2500, 1:1000); Ocln (Invitrogen, 71-1500, 1:1000); and MMP9 (Boster, Shanghai, China; PB9669, 1:1000).

Techniques: Expressing, Control, Western Blot, Immunostaining, Enzyme-linked Immunosorbent Assay, One-tailed Test

Journal: International Journal of Molecular Sciences

Article Title: Mesenchymal Stem Cells Restore Endothelial Integrity and Alleviate Emotional Impairments in a Diabetic Mouse Model via Inhibition of MMP-9 Activity

doi: 10.3390/ijms26073355

Figure Lengend Snippet: Figure 6. MSC treatment attenuated DM-induced neuroinflammation and synaptic deficits. (A) Representative immunofluorescence images of Iba1 staining in the HIP of mice from the CTL, MSC, DM, and DM+MSC groups. The dashed box indicates the magnified region shown in the insets. Scale bars: 100 µm (main images) and 20 µm (insets). (B) Quantitative analysis of Iba1+ microglia in the HIP. n = 4. (C) qPCR analysis of iNOS and Arg1 mRNA levels in the HIP of mice from the CTL, MSC, DM, and DM+MSC groups. n = 9. (D) qPCR analysis of mRNA levels of IL-6, IL-1β, and TNF-α in the HIP of mice from the CTL, MSC, DM, and DM+MSC groups. n = 9. (E) ELISA results showing serum levels of IL-6, IL-1β, and TNF-α in CTL, MSC, DM, and DM+MSC mice. n = 4. (F) Representative WB images of PSD95 and SYN1 expression in the HIP. β-actin was used as a loading control. (G) Semi-quantitative analysis of PSD95 and SYN1 protein levels. n = 6. Data are presented as mean ± SEM. Two-way ANOVA followed by Tukey’s post hoc test. ** p < 0.01, *** p < 0.001.

Article Snippet: The membranes were blocked and incubated overnight with the following antibodies: PSD95 (SYSN, Toronto, ON, Canada, 124002, 1:1000); SYN1 (SYSN, 106011, 1:1000); Cldn5 (Invitrogen, 35-2500, 1:1000); Ocln (Invitrogen, 71-1500, 1:1000); and MMP9 (Boster, Shanghai, China; PB9669, 1:1000).

Techniques: Immunofluorescence, Staining, Enzyme-linked Immunosorbent Assay, Expressing, Control

Journal: STAR Protocols

Article Title: Protocol for generating human cerebral organoids from two-dimensional cultures of pluripotent stem cells bypassing embryoid body aggregation

doi: 10.1016/j.xpro.2025.103678

Figure Lengend Snippet: Characterization of the main cell types in the hCOs at maturation (A) hCOs immunostained for cell cycle marker Ki67 (green) and neural precursor marker Sox2 (green), different neuronal markers (DCX, βIIITubulin and MAP2) (red) and synaptic marker (SYN1) (green). Cell nuclei were identified by Höechst 33258 staining (blue). VZs are marked with white dashed lines. Scale bar = 100 μm. (B) Whole-mount immunohistochemistry of hCOs stained for Sox2 (red) and MAP2 (green). Cell nuclei were identified by Höechst 33258 staining (blue). VZs are marked with white dashed lines. Scale bar = 100 μm. hCOs, human cerebral organoids.

Article Snippet: Rabbit polyclonal anti-Synapsin 1 (Syn1) (1:200) , Merck (Millipore) , Cat#AB1543; RRID: AB_2200400.

Techniques: Marker, Staining, Immunohistochemistry

Journal: STAR Protocols

Article Title: Protocol for generating human cerebral organoids from two-dimensional cultures of pluripotent stem cells bypassing embryoid body aggregation

doi: 10.1016/j.xpro.2025.103678

Figure Lengend Snippet:

Article Snippet: Rabbit polyclonal anti-Synapsin 1 (Syn1) (1:200) , Merck (Millipore) , Cat#AB1543; RRID: AB_2200400.

Techniques: Recombinant, Knock-Out, Software