anti syk Search Results


rea681 i120 722 miltenyi  (Miltenyi Biotec)
94
Miltenyi Biotec rea681 i120 722 miltenyi
Rea681 I120 722 Miltenyi, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti+syk/Syk+pY348+Antibody%2C+anti-human%2C+REAfinity/10__1016_slash_j__celrep__2026__117140-308-24-25
Average 94 stars, based on 1 article reviews
rea681 i120 722 miltenyi - by Bioz Stars, 2026-09
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p syk y323  (Cell Signaling Technology Inc)
93
Cell Signaling Technology Inc p syk y323
Protein and mRNA expression of TULA-2, <t>SYK</t> <t>Y323</t> and VEGFR-2 Y1175 in ECs. ECs were incubated under normal culture conditions. ( A ) RT-qPCR showed no significant change in the mRNA levels of TULA-2 in OECs. ( B ) Western blot analysis revealed an increase in the protein levels of TULA-2 in OECs, and the phosphorylation levels of SYK Y323 and VEGFR-2 Y1175 were decreased in OECs (*P < 0.05, n = 3).
P Syk Y323, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti+syk/Phospho-Syk+(Tyr323)+Antibody/pmc07746355-134-46-52
Average 93 stars, based on 1 article reviews
p syk y323 - by Bioz Stars, 2026-09
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zap70 py493 antibody  (Cell Signaling Technology Inc)
93
Cell Signaling Technology Inc zap70 py493 antibody
(A) Left: cartoon showing a cell culture assay in which PD-1 transduced Jurkat cells were stimulated with three types of antigen-loaded Raji B cells: (1) parental Raji cells that express neither PD-L1 nor PD-1, (2) Raji cells transduced with only PD-L1 (PD-L1+), and (3) Raji transduced with both PD-L1 and PD-1 (PD-L1+/PD-1+). On the right are FACS histograms showing PD-1 and PD-L1 surface expression of parental Jurkat cells, PD-1–mGFP transduced Jurkat (PD-1+) cells, parental Raji cells, PD-L1–mCherry transduced Raji (PD-L1+) cells, and Raji cells co-transduced with both PD-1–mGFP and PD-L1–mCherry (PD-L1+/PD-1+). a.u., arbitrary units. (B–D) Jurkat cells expressing PD-1–mGFP (PD-1+) were conjugated with Raji B cells (parental) (B), Raji cells transduced with only PD-L1–mCherry (PD-L1+) (C), or Raji cells co-transduced with PD-L1–mCherry and unlabeled PD-1 (PD-L1+/PD-1+) (D), as illustrated in the cartoon on the left (CD28 and B7 are omitted in this cartoon for simplicity). Shown on the right are confocal images of the cell conjugate acquired 2 min after cell-cell contact. mGFP and mCherry signals are shown as green and magenta, respectively. Scale bars, 10 µm. (E) Bar graph summarizing the interface enrichment indices (calculated as described in STAR Methods) of the three conditions shown in (B). Data are shown as mean ± SEM; n = 35 cells from three independent experiments. See also . (F) A representative western blot showing the levels <t>ZAP70-Y493</t> phosphorylation and p85 (PI3K regulatory subunit) co-immunoprecipitated (IP) with CD28 from the lysates of the indicated Jurkat-Raji co-culture. Jurkat cells expressing PD-1–mGFP (PD-1+) were stimulated with Raji cells (parental), Raji transduced with PD-L1–mCherry (PD-L1+), or Raji cells co-transduced with PD-L1–mCherry and PD-1–mGFP (PD-L1+/PD-1+); the times at which the co-culture was lysed are indicated (STAR Methods). WCL, whole cell lysate. (G and H) Bar graphs summarizing immunoblots in (F), including <t>pY493-ZAP70</t> immunoblot (G) and CD28 coIP p85 immunoblot (H). The optical density corresponding to each band was quantified by ImageJ and normalized to the conditions in which parental Raji cells were used as the APCs. Data are presented as mean ± SEM from three independent replicates. (I) Bar graph summarizing IL-2 levels in the medium of the indicated Jurkat-Raji co-culture, as described in (B)–(D), 24 hr after cell-cell contact. Data are presented as mean ± SEM from three independent measurements, with each run performed in triplicate.
Zap70 Py493 Antibody, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti+syk/Phospho-Zap-70+(Tyr493)%2FSyk+(Tyr526)+Antibody/pmc06093302-6-0-4
Average 93 stars, based on 1 article reviews
zap70 py493 antibody - by Bioz Stars, 2026-09
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phospho zap 70  (Cell Signaling Technology Inc)
95
Cell Signaling Technology Inc phospho zap 70
(A) Left: cartoon showing a cell culture assay in which PD-1 transduced Jurkat cells were stimulated with three types of antigen-loaded Raji B cells: (1) parental Raji cells that express neither PD-L1 nor PD-1, (2) Raji cells transduced with only PD-L1 (PD-L1+), and (3) Raji transduced with both PD-L1 and PD-1 (PD-L1+/PD-1+). On the right are FACS histograms showing PD-1 and PD-L1 surface expression of parental Jurkat cells, PD-1–mGFP transduced Jurkat (PD-1+) cells, parental Raji cells, PD-L1–mCherry transduced Raji (PD-L1+) cells, and Raji cells co-transduced with both PD-1–mGFP and PD-L1–mCherry (PD-L1+/PD-1+). a.u., arbitrary units. (B–D) Jurkat cells expressing PD-1–mGFP (PD-1+) were conjugated with Raji B cells (parental) (B), Raji cells transduced with only PD-L1–mCherry (PD-L1+) (C), or Raji cells co-transduced with PD-L1–mCherry and unlabeled PD-1 (PD-L1+/PD-1+) (D), as illustrated in the cartoon on the left (CD28 and B7 are omitted in this cartoon for simplicity). Shown on the right are confocal images of the cell conjugate acquired 2 min after cell-cell contact. mGFP and mCherry signals are shown as green and magenta, respectively. Scale bars, 10 µm. (E) Bar graph summarizing the interface enrichment indices (calculated as described in STAR Methods) of the three conditions shown in (B). Data are shown as mean ± SEM; n = 35 cells from three independent experiments. See also . (F) A representative western blot showing the levels <t>ZAP70-Y493</t> phosphorylation and p85 (PI3K regulatory subunit) co-immunoprecipitated (IP) with CD28 from the lysates of the indicated Jurkat-Raji co-culture. Jurkat cells expressing PD-1–mGFP (PD-1+) were stimulated with Raji cells (parental), Raji transduced with PD-L1–mCherry (PD-L1+), or Raji cells co-transduced with PD-L1–mCherry and PD-1–mGFP (PD-L1+/PD-1+); the times at which the co-culture was lysed are indicated (STAR Methods). WCL, whole cell lysate. (G and H) Bar graphs summarizing immunoblots in (F), including <t>pY493-ZAP70</t> immunoblot (G) and CD28 coIP p85 immunoblot (H). The optical density corresponding to each band was quantified by ImageJ and normalized to the conditions in which parental Raji cells were used as the APCs. Data are presented as mean ± SEM from three independent replicates. (I) Bar graph summarizing IL-2 levels in the medium of the indicated Jurkat-Raji co-culture, as described in (B)–(D), 24 hr after cell-cell contact. Data are presented as mean ± SEM from three independent measurements, with each run performed in triplicate.
Phospho Zap 70, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti+syk/Phospho-Zap-70+(Tyr319)%2FSyk+(Tyr352)+Antibody/pmc12887265-14-0-5
Average 95 stars, based on 1 article reviews
phospho zap 70 - by Bioz Stars, 2026-09
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syk  (Cell Signaling Technology Inc)
96
Cell Signaling Technology Inc syk
(A) Left: cartoon showing a cell culture assay in which PD-1 transduced Jurkat cells were stimulated with three types of antigen-loaded Raji B cells: (1) parental Raji cells that express neither PD-L1 nor PD-1, (2) Raji cells transduced with only PD-L1 (PD-L1+), and (3) Raji transduced with both PD-L1 and PD-1 (PD-L1+/PD-1+). On the right are FACS histograms showing PD-1 and PD-L1 surface expression of parental Jurkat cells, PD-1–mGFP transduced Jurkat (PD-1+) cells, parental Raji cells, PD-L1–mCherry transduced Raji (PD-L1+) cells, and Raji cells co-transduced with both PD-1–mGFP and PD-L1–mCherry (PD-L1+/PD-1+). a.u., arbitrary units. (B–D) Jurkat cells expressing PD-1–mGFP (PD-1+) were conjugated with Raji B cells (parental) (B), Raji cells transduced with only PD-L1–mCherry (PD-L1+) (C), or Raji cells co-transduced with PD-L1–mCherry and unlabeled PD-1 (PD-L1+/PD-1+) (D), as illustrated in the cartoon on the left (CD28 and B7 are omitted in this cartoon for simplicity). Shown on the right are confocal images of the cell conjugate acquired 2 min after cell-cell contact. mGFP and mCherry signals are shown as green and magenta, respectively. Scale bars, 10 µm. (E) Bar graph summarizing the interface enrichment indices (calculated as described in STAR Methods) of the three conditions shown in (B). Data are shown as mean ± SEM; n = 35 cells from three independent experiments. See also . (F) A representative western blot showing the levels <t>ZAP70-Y493</t> phosphorylation and p85 (PI3K regulatory subunit) co-immunoprecipitated (IP) with CD28 from the lysates of the indicated Jurkat-Raji co-culture. Jurkat cells expressing PD-1–mGFP (PD-1+) were stimulated with Raji cells (parental), Raji transduced with PD-L1–mCherry (PD-L1+), or Raji cells co-transduced with PD-L1–mCherry and PD-1–mGFP (PD-L1+/PD-1+); the times at which the co-culture was lysed are indicated (STAR Methods). WCL, whole cell lysate. (G and H) Bar graphs summarizing immunoblots in (F), including <t>pY493-ZAP70</t> immunoblot (G) and CD28 coIP p85 immunoblot (H). The optical density corresponding to each band was quantified by ImageJ and normalized to the conditions in which parental Raji cells were used as the APCs. Data are presented as mean ± SEM from three independent replicates. (I) Bar graph summarizing IL-2 levels in the medium of the indicated Jurkat-Raji co-culture, as described in (B)–(D), 24 hr after cell-cell contact. Data are presented as mean ± SEM from three independent measurements, with each run performed in triplicate.
Syk, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti+syk/Syk+Antibody/10__1177_slash_1934578x1601100930-59-33-38
Average 96 stars, based on 1 article reviews
syk - by Bioz Stars, 2026-09
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rabbit anti psyk  (Cell Signaling Technology Inc)
95
Cell Signaling Technology Inc rabbit anti psyk
(A) Left: cartoon showing a cell culture assay in which PD-1 transduced Jurkat cells were stimulated with three types of antigen-loaded Raji B cells: (1) parental Raji cells that express neither PD-L1 nor PD-1, (2) Raji cells transduced with only PD-L1 (PD-L1+), and (3) Raji transduced with both PD-L1 and PD-1 (PD-L1+/PD-1+). On the right are FACS histograms showing PD-1 and PD-L1 surface expression of parental Jurkat cells, PD-1–mGFP transduced Jurkat (PD-1+) cells, parental Raji cells, PD-L1–mCherry transduced Raji (PD-L1+) cells, and Raji cells co-transduced with both PD-1–mGFP and PD-L1–mCherry (PD-L1+/PD-1+). a.u., arbitrary units. (B–D) Jurkat cells expressing PD-1–mGFP (PD-1+) were conjugated with Raji B cells (parental) (B), Raji cells transduced with only PD-L1–mCherry (PD-L1+) (C), or Raji cells co-transduced with PD-L1–mCherry and unlabeled PD-1 (PD-L1+/PD-1+) (D), as illustrated in the cartoon on the left (CD28 and B7 are omitted in this cartoon for simplicity). Shown on the right are confocal images of the cell conjugate acquired 2 min after cell-cell contact. mGFP and mCherry signals are shown as green and magenta, respectively. Scale bars, 10 µm. (E) Bar graph summarizing the interface enrichment indices (calculated as described in STAR Methods) of the three conditions shown in (B). Data are shown as mean ± SEM; n = 35 cells from three independent experiments. See also . (F) A representative western blot showing the levels <t>ZAP70-Y493</t> phosphorylation and p85 (PI3K regulatory subunit) co-immunoprecipitated (IP) with CD28 from the lysates of the indicated Jurkat-Raji co-culture. Jurkat cells expressing PD-1–mGFP (PD-1+) were stimulated with Raji cells (parental), Raji transduced with PD-L1–mCherry (PD-L1+), or Raji cells co-transduced with PD-L1–mCherry and PD-1–mGFP (PD-L1+/PD-1+); the times at which the co-culture was lysed are indicated (STAR Methods). WCL, whole cell lysate. (G and H) Bar graphs summarizing immunoblots in (F), including <t>pY493-ZAP70</t> immunoblot (G) and CD28 coIP p85 immunoblot (H). The optical density corresponding to each band was quantified by ImageJ and normalized to the conditions in which parental Raji cells were used as the APCs. Data are presented as mean ± SEM from three independent replicates. (I) Bar graph summarizing IL-2 levels in the medium of the indicated Jurkat-Raji co-culture, as described in (B)–(D), 24 hr after cell-cell contact. Data are presented as mean ± SEM from three independent measurements, with each run performed in triplicate.
Rabbit Anti Psyk, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti+syk/Phospho-Syk+(Tyr525%2F526)+Antibody/pm28409541-31-20-24
Average 95 stars, based on 1 article reviews
rabbit anti psyk - by Bioz Stars, 2026-09
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syk  (Santa Cruz Biotechnology)
95
Santa Cruz Biotechnology syk
(A) Left: cartoon showing a cell culture assay in which PD-1 transduced Jurkat cells were stimulated with three types of antigen-loaded Raji B cells: (1) parental Raji cells that express neither PD-L1 nor PD-1, (2) Raji cells transduced with only PD-L1 (PD-L1+), and (3) Raji transduced with both PD-L1 and PD-1 (PD-L1+/PD-1+). On the right are FACS histograms showing PD-1 and PD-L1 surface expression of parental Jurkat cells, PD-1–mGFP transduced Jurkat (PD-1+) cells, parental Raji cells, PD-L1–mCherry transduced Raji (PD-L1+) cells, and Raji cells co-transduced with both PD-1–mGFP and PD-L1–mCherry (PD-L1+/PD-1+). a.u., arbitrary units. (B–D) Jurkat cells expressing PD-1–mGFP (PD-1+) were conjugated with Raji B cells (parental) (B), Raji cells transduced with only PD-L1–mCherry (PD-L1+) (C), or Raji cells co-transduced with PD-L1–mCherry and unlabeled PD-1 (PD-L1+/PD-1+) (D), as illustrated in the cartoon on the left (CD28 and B7 are omitted in this cartoon for simplicity). Shown on the right are confocal images of the cell conjugate acquired 2 min after cell-cell contact. mGFP and mCherry signals are shown as green and magenta, respectively. Scale bars, 10 µm. (E) Bar graph summarizing the interface enrichment indices (calculated as described in STAR Methods) of the three conditions shown in (B). Data are shown as mean ± SEM; n = 35 cells from three independent experiments. See also . (F) A representative western blot showing the levels <t>ZAP70-Y493</t> phosphorylation and p85 (PI3K regulatory subunit) co-immunoprecipitated (IP) with CD28 from the lysates of the indicated Jurkat-Raji co-culture. Jurkat cells expressing PD-1–mGFP (PD-1+) were stimulated with Raji cells (parental), Raji transduced with PD-L1–mCherry (PD-L1+), or Raji cells co-transduced with PD-L1–mCherry and PD-1–mGFP (PD-L1+/PD-1+); the times at which the co-culture was lysed are indicated (STAR Methods). WCL, whole cell lysate. (G and H) Bar graphs summarizing immunoblots in (F), including <t>pY493-ZAP70</t> immunoblot (G) and CD28 coIP p85 immunoblot (H). The optical density corresponding to each band was quantified by ImageJ and normalized to the conditions in which parental Raji cells were used as the APCs. Data are presented as mean ± SEM from three independent replicates. (I) Bar graph summarizing IL-2 levels in the medium of the indicated Jurkat-Raji co-culture, as described in (B)–(D), 24 hr after cell-cell contact. Data are presented as mean ± SEM from three independent measurements, with each run performed in triplicate.
Syk, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti+syk/Syk+Antibody/pm41871717-58-115-121
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syk - by Bioz Stars, 2026-09
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phospho smad antibody sampler kit  (Cell Signaling Technology Inc)
93
Cell Signaling Technology Inc phospho smad antibody sampler kit
(A) Left: cartoon showing a cell culture assay in which PD-1 transduced Jurkat cells were stimulated with three types of antigen-loaded Raji B cells: (1) parental Raji cells that express neither PD-L1 nor PD-1, (2) Raji cells transduced with only PD-L1 (PD-L1+), and (3) Raji transduced with both PD-L1 and PD-1 (PD-L1+/PD-1+). On the right are FACS histograms showing PD-1 and PD-L1 surface expression of parental Jurkat cells, PD-1–mGFP transduced Jurkat (PD-1+) cells, parental Raji cells, PD-L1–mCherry transduced Raji (PD-L1+) cells, and Raji cells co-transduced with both PD-1–mGFP and PD-L1–mCherry (PD-L1+/PD-1+). a.u., arbitrary units. (B–D) Jurkat cells expressing PD-1–mGFP (PD-1+) were conjugated with Raji B cells (parental) (B), Raji cells transduced with only PD-L1–mCherry (PD-L1+) (C), or Raji cells co-transduced with PD-L1–mCherry and unlabeled PD-1 (PD-L1+/PD-1+) (D), as illustrated in the cartoon on the left (CD28 and B7 are omitted in this cartoon for simplicity). Shown on the right are confocal images of the cell conjugate acquired 2 min after cell-cell contact. mGFP and mCherry signals are shown as green and magenta, respectively. Scale bars, 10 µm. (E) Bar graph summarizing the interface enrichment indices (calculated as described in STAR Methods) of the three conditions shown in (B). Data are shown as mean ± SEM; n = 35 cells from three independent experiments. See also . (F) A representative western blot showing the levels <t>ZAP70-Y493</t> phosphorylation and p85 (PI3K regulatory subunit) co-immunoprecipitated (IP) with CD28 from the lysates of the indicated Jurkat-Raji co-culture. Jurkat cells expressing PD-1–mGFP (PD-1+) were stimulated with Raji cells (parental), Raji transduced with PD-L1–mCherry (PD-L1+), or Raji cells co-transduced with PD-L1–mCherry and PD-1–mGFP (PD-L1+/PD-1+); the times at which the co-culture was lysed are indicated (STAR Methods). WCL, whole cell lysate. (G and H) Bar graphs summarizing immunoblots in (F), including <t>pY493-ZAP70</t> immunoblot (G) and CD28 coIP p85 immunoblot (H). The optical density corresponding to each band was quantified by ImageJ and normalized to the conditions in which parental Raji cells were used as the APCs. Data are presented as mean ± SEM from three independent replicates. (I) Bar graph summarizing IL-2 levels in the medium of the indicated Jurkat-Raji co-culture, as described in (B)–(D), 24 hr after cell-cell contact. Data are presented as mean ± SEM from three independent measurements, with each run performed in triplicate.
Phospho Smad Antibody Sampler Kit, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti+syk/Phospho-Syk+Antibody+Sampler+Kit/pmc04038615-88-23-27
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1 ap  (Proteintech)
93
Proteintech 1 ap
(A) Left: cartoon showing a cell culture assay in which PD-1 transduced Jurkat cells were stimulated with three types of antigen-loaded Raji B cells: (1) parental Raji cells that express neither PD-L1 nor PD-1, (2) Raji cells transduced with only PD-L1 (PD-L1+), and (3) Raji transduced with both PD-L1 and PD-1 (PD-L1+/PD-1+). On the right are FACS histograms showing PD-1 and PD-L1 surface expression of parental Jurkat cells, PD-1–mGFP transduced Jurkat (PD-1+) cells, parental Raji cells, PD-L1–mCherry transduced Raji (PD-L1+) cells, and Raji cells co-transduced with both PD-1–mGFP and PD-L1–mCherry (PD-L1+/PD-1+). a.u., arbitrary units. (B–D) Jurkat cells expressing PD-1–mGFP (PD-1+) were conjugated with Raji B cells (parental) (B), Raji cells transduced with only PD-L1–mCherry (PD-L1+) (C), or Raji cells co-transduced with PD-L1–mCherry and unlabeled PD-1 (PD-L1+/PD-1+) (D), as illustrated in the cartoon on the left (CD28 and B7 are omitted in this cartoon for simplicity). Shown on the right are confocal images of the cell conjugate acquired 2 min after cell-cell contact. mGFP and mCherry signals are shown as green and magenta, respectively. Scale bars, 10 µm. (E) Bar graph summarizing the interface enrichment indices (calculated as described in STAR Methods) of the three conditions shown in (B). Data are shown as mean ± SEM; n = 35 cells from three independent experiments. See also . (F) A representative western blot showing the levels <t>ZAP70-Y493</t> phosphorylation and p85 (PI3K regulatory subunit) co-immunoprecipitated (IP) with CD28 from the lysates of the indicated Jurkat-Raji co-culture. Jurkat cells expressing PD-1–mGFP (PD-1+) were stimulated with Raji cells (parental), Raji transduced with PD-L1–mCherry (PD-L1+), or Raji cells co-transduced with PD-L1–mCherry and PD-1–mGFP (PD-L1+/PD-1+); the times at which the co-culture was lysed are indicated (STAR Methods). WCL, whole cell lysate. (G and H) Bar graphs summarizing immunoblots in (F), including <t>pY493-ZAP70</t> immunoblot (G) and CD28 coIP p85 immunoblot (H). The optical density corresponding to each band was quantified by ImageJ and normalized to the conditions in which parental Raji cells were used as the APCs. Data are presented as mean ± SEM from three independent replicates. (I) Bar graph summarizing IL-2 levels in the medium of the indicated Jurkat-Raji co-culture, as described in (B)–(D), 24 hr after cell-cell contact. Data are presented as mean ± SEM from three independent measurements, with each run performed in triplicate.
1 Ap, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti+syk/SYK+Antibody/pmc08331038-16-2-15
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1 ap - by Bioz Stars, 2026-09
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syk  (Novus Biologicals)
92
Novus Biologicals syk
(A) Left: cartoon showing a cell culture assay in which PD-1 transduced Jurkat cells were stimulated with three types of antigen-loaded Raji B cells: (1) parental Raji cells that express neither PD-L1 nor PD-1, (2) Raji cells transduced with only PD-L1 (PD-L1+), and (3) Raji transduced with both PD-L1 and PD-1 (PD-L1+/PD-1+). On the right are FACS histograms showing PD-1 and PD-L1 surface expression of parental Jurkat cells, PD-1–mGFP transduced Jurkat (PD-1+) cells, parental Raji cells, PD-L1–mCherry transduced Raji (PD-L1+) cells, and Raji cells co-transduced with both PD-1–mGFP and PD-L1–mCherry (PD-L1+/PD-1+). a.u., arbitrary units. (B–D) Jurkat cells expressing PD-1–mGFP (PD-1+) were conjugated with Raji B cells (parental) (B), Raji cells transduced with only PD-L1–mCherry (PD-L1+) (C), or Raji cells co-transduced with PD-L1–mCherry and unlabeled PD-1 (PD-L1+/PD-1+) (D), as illustrated in the cartoon on the left (CD28 and B7 are omitted in this cartoon for simplicity). Shown on the right are confocal images of the cell conjugate acquired 2 min after cell-cell contact. mGFP and mCherry signals are shown as green and magenta, respectively. Scale bars, 10 µm. (E) Bar graph summarizing the interface enrichment indices (calculated as described in STAR Methods) of the three conditions shown in (B). Data are shown as mean ± SEM; n = 35 cells from three independent experiments. See also . (F) A representative western blot showing the levels <t>ZAP70-Y493</t> phosphorylation and p85 (PI3K regulatory subunit) co-immunoprecipitated (IP) with CD28 from the lysates of the indicated Jurkat-Raji co-culture. Jurkat cells expressing PD-1–mGFP (PD-1+) were stimulated with Raji cells (parental), Raji transduced with PD-L1–mCherry (PD-L1+), or Raji cells co-transduced with PD-L1–mCherry and PD-1–mGFP (PD-L1+/PD-1+); the times at which the co-culture was lysed are indicated (STAR Methods). WCL, whole cell lysate. (G and H) Bar graphs summarizing immunoblots in (F), including <t>pY493-ZAP70</t> immunoblot (G) and CD28 coIP p85 immunoblot (H). The optical density corresponding to each band was quantified by ImageJ and normalized to the conditions in which parental Raji cells were used as the APCs. Data are presented as mean ± SEM from three independent replicates. (I) Bar graph summarizing IL-2 levels in the medium of the indicated Jurkat-Raji co-culture, as described in (B)–(D), 24 hr after cell-cell contact. Data are presented as mean ± SEM from three independent measurements, with each run performed in triplicate.
Syk, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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phospho syk ser297  (Cell Signaling Technology Inc)
92
Cell Signaling Technology Inc phospho syk ser297

Phospho Syk Ser297, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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anti syk  (Novus Biologicals)
90
Novus Biologicals anti syk

Anti Syk, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Protein and mRNA expression of TULA-2, SYK Y323 and VEGFR-2 Y1175 in ECs. ECs were incubated under normal culture conditions. ( A ) RT-qPCR showed no significant change in the mRNA levels of TULA-2 in OECs. ( B ) Western blot analysis revealed an increase in the protein levels of TULA-2 in OECs, and the phosphorylation levels of SYK Y323 and VEGFR-2 Y1175 were decreased in OECs (*P < 0.05, n = 3).

Journal: Aging (Albany NY)

Article Title: miR-25-3p promotes endothelial cell angiogenesis in aging mice via TULA-2/SYK/VEGFR-2 downregulation

doi: 10.18632/aging.103834

Figure Lengend Snippet: Protein and mRNA expression of TULA-2, SYK Y323 and VEGFR-2 Y1175 in ECs. ECs were incubated under normal culture conditions. ( A ) RT-qPCR showed no significant change in the mRNA levels of TULA-2 in OECs. ( B ) Western blot analysis revealed an increase in the protein levels of TULA-2 in OECs, and the phosphorylation levels of SYK Y323 and VEGFR-2 Y1175 were decreased in OECs (*P < 0.05, n = 3).

Article Snippet: After blocking with TBST supplemented with 5% bovine serum albumin (BSA) (BioFroxx Inc, Germany), the PVDF membrane was incubated at 4°C overnight with primary antibodies against mouse TULA-2 (1:500; cat. no. sc-514612; Santa Cruz, CA, USA), SYK (1:1000; cat. no. 13198; Cell Signaling Technologies, Beverly, USA), p-SYK Y323 (1:1000; cat. no. 2715; Cell Signaling Technologies), VEGFR-2 (1:1000; cat. no. 9698; Cell Signaling Technologies), p-VEGFR-2 Y1175 (1:1000; cat. no. 2478; Cell Signaling Technologies) and GAPDH (1:1000; cat. no. 5174; Cell Signaling Technologies).

Techniques: Expressing, Incubation, Quantitative RT-PCR, Western Blot, Phospho-proteomics

The miR-25-3p inhibitor negatively regulates the angiogenic signaling pathway by reducing angiogenic growth factor expression. ( A ) RT-qPCR showed no significant change in the TULA-2 mRNA level in YECs after transfection with the miR-25-3p inhibitor. ( B ) Western blot analysis showed that the miR-25-3p inhibitor upregulated the protein level of TULA-2 and downregulated the phosphorylation levels of SYK Y323 and VEGFR-2 Y1175 in YECs (n=3, data are expressed as the mean ± SEM, *P < 0.05 versus the negative control).

Journal: Aging (Albany NY)

Article Title: miR-25-3p promotes endothelial cell angiogenesis in aging mice via TULA-2/SYK/VEGFR-2 downregulation

doi: 10.18632/aging.103834

Figure Lengend Snippet: The miR-25-3p inhibitor negatively regulates the angiogenic signaling pathway by reducing angiogenic growth factor expression. ( A ) RT-qPCR showed no significant change in the TULA-2 mRNA level in YECs after transfection with the miR-25-3p inhibitor. ( B ) Western blot analysis showed that the miR-25-3p inhibitor upregulated the protein level of TULA-2 and downregulated the phosphorylation levels of SYK Y323 and VEGFR-2 Y1175 in YECs (n=3, data are expressed as the mean ± SEM, *P < 0.05 versus the negative control).

Article Snippet: After blocking with TBST supplemented with 5% bovine serum albumin (BSA) (BioFroxx Inc, Germany), the PVDF membrane was incubated at 4°C overnight with primary antibodies against mouse TULA-2 (1:500; cat. no. sc-514612; Santa Cruz, CA, USA), SYK (1:1000; cat. no. 13198; Cell Signaling Technologies, Beverly, USA), p-SYK Y323 (1:1000; cat. no. 2715; Cell Signaling Technologies), VEGFR-2 (1:1000; cat. no. 9698; Cell Signaling Technologies), p-VEGFR-2 Y1175 (1:1000; cat. no. 2478; Cell Signaling Technologies) and GAPDH (1:1000; cat. no. 5174; Cell Signaling Technologies).

Techniques: Expressing, Quantitative RT-PCR, Transfection, Western Blot, Phospho-proteomics, Negative Control

MiR-25-3p mimics positively regulate the angiogenic signaling pathway by increasing angiogenic growth factor expression. ( A ) RT-qPCR showed no significant change in the TULA-2 mRNA level in OECs after transfection with miR-25-3p mimics. ( B ) Western blot analysis showed that miR-25-3p mimics downregulated the protein level of TULA-2 and upregulated the phosphorylation levels of SYK Y323 and VEGFR-2 Y1175 in OECs (n=3, data are expressed as the mean ± SEM, *P < 0.05 versus the negative control).

Journal: Aging (Albany NY)

Article Title: miR-25-3p promotes endothelial cell angiogenesis in aging mice via TULA-2/SYK/VEGFR-2 downregulation

doi: 10.18632/aging.103834

Figure Lengend Snippet: MiR-25-3p mimics positively regulate the angiogenic signaling pathway by increasing angiogenic growth factor expression. ( A ) RT-qPCR showed no significant change in the TULA-2 mRNA level in OECs after transfection with miR-25-3p mimics. ( B ) Western blot analysis showed that miR-25-3p mimics downregulated the protein level of TULA-2 and upregulated the phosphorylation levels of SYK Y323 and VEGFR-2 Y1175 in OECs (n=3, data are expressed as the mean ± SEM, *P < 0.05 versus the negative control).

Article Snippet: After blocking with TBST supplemented with 5% bovine serum albumin (BSA) (BioFroxx Inc, Germany), the PVDF membrane was incubated at 4°C overnight with primary antibodies against mouse TULA-2 (1:500; cat. no. sc-514612; Santa Cruz, CA, USA), SYK (1:1000; cat. no. 13198; Cell Signaling Technologies, Beverly, USA), p-SYK Y323 (1:1000; cat. no. 2715; Cell Signaling Technologies), VEGFR-2 (1:1000; cat. no. 9698; Cell Signaling Technologies), p-VEGFR-2 Y1175 (1:1000; cat. no. 2478; Cell Signaling Technologies) and GAPDH (1:1000; cat. no. 5174; Cell Signaling Technologies).

Techniques: Expressing, Quantitative RT-PCR, Transfection, Western Blot, Phospho-proteomics, Negative Control

TULA-2 knockdown positively regulates angiogenesis in vitro and increases p-SYK Y323 and p-VEGFR-2 Y1175 expression at the protein level. ( A ) Transwell assays showed that TULA-2 siRNA promoted the migration ability of OECs, and co-transfection of OECs with both si-TULA-2 and miR-25-3p inhibitors did not suppress the effect of TULA-2 upregulation on EC migration relative to the effects of siRNA-NC (n=5; Scale bar, 100 μm). ( B ) Tube formation determined on Matrigel showed that TULA-2 siRNA increased the total tube length, and co-transfection of OECs with both si-TULA-2 and miR-25-3p inhibitors did not suppress the effect of TULA-2 upregulation on EC tube formation relative to the effects of siRNA-NC (n=4; scale bar, 100 μm). ( C ) Western blot analysis of relative TULA-2, SYK Y323 and VEGFR-2 Y1175 expression in OECs transfected with the negative control, si-TULA-2, si-TULA-2+ miR-25-3p inhibitor and miR-25-3p inhibitor (data are expressed as the mean ± SEM, *P < 0.05, **P < 0.01 versus the negative control).

Journal: Aging (Albany NY)

Article Title: miR-25-3p promotes endothelial cell angiogenesis in aging mice via TULA-2/SYK/VEGFR-2 downregulation

doi: 10.18632/aging.103834

Figure Lengend Snippet: TULA-2 knockdown positively regulates angiogenesis in vitro and increases p-SYK Y323 and p-VEGFR-2 Y1175 expression at the protein level. ( A ) Transwell assays showed that TULA-2 siRNA promoted the migration ability of OECs, and co-transfection of OECs with both si-TULA-2 and miR-25-3p inhibitors did not suppress the effect of TULA-2 upregulation on EC migration relative to the effects of siRNA-NC (n=5; Scale bar, 100 μm). ( B ) Tube formation determined on Matrigel showed that TULA-2 siRNA increased the total tube length, and co-transfection of OECs with both si-TULA-2 and miR-25-3p inhibitors did not suppress the effect of TULA-2 upregulation on EC tube formation relative to the effects of siRNA-NC (n=4; scale bar, 100 μm). ( C ) Western blot analysis of relative TULA-2, SYK Y323 and VEGFR-2 Y1175 expression in OECs transfected with the negative control, si-TULA-2, si-TULA-2+ miR-25-3p inhibitor and miR-25-3p inhibitor (data are expressed as the mean ± SEM, *P < 0.05, **P < 0.01 versus the negative control).

Article Snippet: After blocking with TBST supplemented with 5% bovine serum albumin (BSA) (BioFroxx Inc, Germany), the PVDF membrane was incubated at 4°C overnight with primary antibodies against mouse TULA-2 (1:500; cat. no. sc-514612; Santa Cruz, CA, USA), SYK (1:1000; cat. no. 13198; Cell Signaling Technologies, Beverly, USA), p-SYK Y323 (1:1000; cat. no. 2715; Cell Signaling Technologies), VEGFR-2 (1:1000; cat. no. 9698; Cell Signaling Technologies), p-VEGFR-2 Y1175 (1:1000; cat. no. 2478; Cell Signaling Technologies) and GAPDH (1:1000; cat. no. 5174; Cell Signaling Technologies).

Techniques: Knockdown, In Vitro, Expressing, Migration, Cotransfection, Western Blot, Transfection, Negative Control

The interaction of TULA-2 and SYK inhibits the phosphorylation of SYK Y323 and VEGFR-2 Y1175. ( A ) Western blot analysis of the Co-IP experiment showed that SYK immunoprecipitated with anti-TULA-2, SYK and TULA-2, which was visualized by Western blot. ( B ) Western blot analysis of relative TULA-2, SYK Y323 and VEGFR-2 Y1175 expression in YECs transfected with the control, BAY 61-3606 (SYK inhibitor), and BAY 61-3606 + si-TULA-2. The phosphorylation levels of SYK Y323 and VEGFR-2 Y1175 were decreased in YECs after transfection with BAY 61-3606 or BAY 61-3606 + si-TULA-2 (data are expressed as the mean ± SEM, *P < 0.05 versus the negative control).

Journal: Aging (Albany NY)

Article Title: miR-25-3p promotes endothelial cell angiogenesis in aging mice via TULA-2/SYK/VEGFR-2 downregulation

doi: 10.18632/aging.103834

Figure Lengend Snippet: The interaction of TULA-2 and SYK inhibits the phosphorylation of SYK Y323 and VEGFR-2 Y1175. ( A ) Western blot analysis of the Co-IP experiment showed that SYK immunoprecipitated with anti-TULA-2, SYK and TULA-2, which was visualized by Western blot. ( B ) Western blot analysis of relative TULA-2, SYK Y323 and VEGFR-2 Y1175 expression in YECs transfected with the control, BAY 61-3606 (SYK inhibitor), and BAY 61-3606 + si-TULA-2. The phosphorylation levels of SYK Y323 and VEGFR-2 Y1175 were decreased in YECs after transfection with BAY 61-3606 or BAY 61-3606 + si-TULA-2 (data are expressed as the mean ± SEM, *P < 0.05 versus the negative control).

Article Snippet: After blocking with TBST supplemented with 5% bovine serum albumin (BSA) (BioFroxx Inc, Germany), the PVDF membrane was incubated at 4°C overnight with primary antibodies against mouse TULA-2 (1:500; cat. no. sc-514612; Santa Cruz, CA, USA), SYK (1:1000; cat. no. 13198; Cell Signaling Technologies, Beverly, USA), p-SYK Y323 (1:1000; cat. no. 2715; Cell Signaling Technologies), VEGFR-2 (1:1000; cat. no. 9698; Cell Signaling Technologies), p-VEGFR-2 Y1175 (1:1000; cat. no. 2478; Cell Signaling Technologies) and GAPDH (1:1000; cat. no. 5174; Cell Signaling Technologies).

Techniques: Phospho-proteomics, Western Blot, Co-Immunoprecipitation Assay, Immunoprecipitation, Expressing, Transfection, Control, Negative Control

The proposed novel mechanism by which miR-25-3p affects angiogenesis in aged mice via the TULA-2/SYK/VEGFR-2 pathway. Aged ECs utilize miR-25-3p to target TULA-2 as a downstream effector to transduce signals, further downregulating and suppressing the phosphorylation of SYK Y323 and VEGFR-2 Y1175 and thus suppressing the angiogenesis pathway and inhibiting the proliferation, migration and tube formation abilities of aged ECs.

Journal: Aging (Albany NY)

Article Title: miR-25-3p promotes endothelial cell angiogenesis in aging mice via TULA-2/SYK/VEGFR-2 downregulation

doi: 10.18632/aging.103834

Figure Lengend Snippet: The proposed novel mechanism by which miR-25-3p affects angiogenesis in aged mice via the TULA-2/SYK/VEGFR-2 pathway. Aged ECs utilize miR-25-3p to target TULA-2 as a downstream effector to transduce signals, further downregulating and suppressing the phosphorylation of SYK Y323 and VEGFR-2 Y1175 and thus suppressing the angiogenesis pathway and inhibiting the proliferation, migration and tube formation abilities of aged ECs.

Article Snippet: After blocking with TBST supplemented with 5% bovine serum albumin (BSA) (BioFroxx Inc, Germany), the PVDF membrane was incubated at 4°C overnight with primary antibodies against mouse TULA-2 (1:500; cat. no. sc-514612; Santa Cruz, CA, USA), SYK (1:1000; cat. no. 13198; Cell Signaling Technologies, Beverly, USA), p-SYK Y323 (1:1000; cat. no. 2715; Cell Signaling Technologies), VEGFR-2 (1:1000; cat. no. 9698; Cell Signaling Technologies), p-VEGFR-2 Y1175 (1:1000; cat. no. 2478; Cell Signaling Technologies) and GAPDH (1:1000; cat. no. 5174; Cell Signaling Technologies).

Techniques: Transduction, Phospho-proteomics, Migration

(A) Left: cartoon showing a cell culture assay in which PD-1 transduced Jurkat cells were stimulated with three types of antigen-loaded Raji B cells: (1) parental Raji cells that express neither PD-L1 nor PD-1, (2) Raji cells transduced with only PD-L1 (PD-L1+), and (3) Raji transduced with both PD-L1 and PD-1 (PD-L1+/PD-1+). On the right are FACS histograms showing PD-1 and PD-L1 surface expression of parental Jurkat cells, PD-1–mGFP transduced Jurkat (PD-1+) cells, parental Raji cells, PD-L1–mCherry transduced Raji (PD-L1+) cells, and Raji cells co-transduced with both PD-1–mGFP and PD-L1–mCherry (PD-L1+/PD-1+). a.u., arbitrary units. (B–D) Jurkat cells expressing PD-1–mGFP (PD-1+) were conjugated with Raji B cells (parental) (B), Raji cells transduced with only PD-L1–mCherry (PD-L1+) (C), or Raji cells co-transduced with PD-L1–mCherry and unlabeled PD-1 (PD-L1+/PD-1+) (D), as illustrated in the cartoon on the left (CD28 and B7 are omitted in this cartoon for simplicity). Shown on the right are confocal images of the cell conjugate acquired 2 min after cell-cell contact. mGFP and mCherry signals are shown as green and magenta, respectively. Scale bars, 10 µm. (E) Bar graph summarizing the interface enrichment indices (calculated as described in STAR Methods) of the three conditions shown in (B). Data are shown as mean ± SEM; n = 35 cells from three independent experiments. See also . (F) A representative western blot showing the levels ZAP70-Y493 phosphorylation and p85 (PI3K regulatory subunit) co-immunoprecipitated (IP) with CD28 from the lysates of the indicated Jurkat-Raji co-culture. Jurkat cells expressing PD-1–mGFP (PD-1+) were stimulated with Raji cells (parental), Raji transduced with PD-L1–mCherry (PD-L1+), or Raji cells co-transduced with PD-L1–mCherry and PD-1–mGFP (PD-L1+/PD-1+); the times at which the co-culture was lysed are indicated (STAR Methods). WCL, whole cell lysate. (G and H) Bar graphs summarizing immunoblots in (F), including pY493-ZAP70 immunoblot (G) and CD28 coIP p85 immunoblot (H). The optical density corresponding to each band was quantified by ImageJ and normalized to the conditions in which parental Raji cells were used as the APCs. Data are presented as mean ± SEM from three independent replicates. (I) Bar graph summarizing IL-2 levels in the medium of the indicated Jurkat-Raji co-culture, as described in (B)–(D), 24 hr after cell-cell contact. Data are presented as mean ± SEM from three independent measurements, with each run performed in triplicate.

Journal: Cell reports

Article Title: Antigen-Presenting Cell-Intrinsic PD-1 Neutralizes PD-L1 in cis to Attenuate PD-1 Signaling in T Cells

doi: 10.1016/j.celrep.2018.06.054

Figure Lengend Snippet: (A) Left: cartoon showing a cell culture assay in which PD-1 transduced Jurkat cells were stimulated with three types of antigen-loaded Raji B cells: (1) parental Raji cells that express neither PD-L1 nor PD-1, (2) Raji cells transduced with only PD-L1 (PD-L1+), and (3) Raji transduced with both PD-L1 and PD-1 (PD-L1+/PD-1+). On the right are FACS histograms showing PD-1 and PD-L1 surface expression of parental Jurkat cells, PD-1–mGFP transduced Jurkat (PD-1+) cells, parental Raji cells, PD-L1–mCherry transduced Raji (PD-L1+) cells, and Raji cells co-transduced with both PD-1–mGFP and PD-L1–mCherry (PD-L1+/PD-1+). a.u., arbitrary units. (B–D) Jurkat cells expressing PD-1–mGFP (PD-1+) were conjugated with Raji B cells (parental) (B), Raji cells transduced with only PD-L1–mCherry (PD-L1+) (C), or Raji cells co-transduced with PD-L1–mCherry and unlabeled PD-1 (PD-L1+/PD-1+) (D), as illustrated in the cartoon on the left (CD28 and B7 are omitted in this cartoon for simplicity). Shown on the right are confocal images of the cell conjugate acquired 2 min after cell-cell contact. mGFP and mCherry signals are shown as green and magenta, respectively. Scale bars, 10 µm. (E) Bar graph summarizing the interface enrichment indices (calculated as described in STAR Methods) of the three conditions shown in (B). Data are shown as mean ± SEM; n = 35 cells from three independent experiments. See also . (F) A representative western blot showing the levels ZAP70-Y493 phosphorylation and p85 (PI3K regulatory subunit) co-immunoprecipitated (IP) with CD28 from the lysates of the indicated Jurkat-Raji co-culture. Jurkat cells expressing PD-1–mGFP (PD-1+) were stimulated with Raji cells (parental), Raji transduced with PD-L1–mCherry (PD-L1+), or Raji cells co-transduced with PD-L1–mCherry and PD-1–mGFP (PD-L1+/PD-1+); the times at which the co-culture was lysed are indicated (STAR Methods). WCL, whole cell lysate. (G and H) Bar graphs summarizing immunoblots in (F), including pY493-ZAP70 immunoblot (G) and CD28 coIP p85 immunoblot (H). The optical density corresponding to each band was quantified by ImageJ and normalized to the conditions in which parental Raji cells were used as the APCs. Data are presented as mean ± SEM from three independent replicates. (I) Bar graph summarizing IL-2 levels in the medium of the indicated Jurkat-Raji co-culture, as described in (B)–(D), 24 hr after cell-cell contact. Data are presented as mean ± SEM from three independent measurements, with each run performed in triplicate.

Article Snippet: ZAP70 pY493 antibody , Cell Signaling Technology , Cat # 2704S; RRID: AB_2217457.

Techniques: Cell Culture, Transduction, Expressing, Western Blot, Phospho-proteomics, Immunoprecipitation, Co-Culture Assay

KEY RESOURCES TABLE

Journal: Cell reports

Article Title: Antigen-Presenting Cell-Intrinsic PD-1 Neutralizes PD-L1 in cis to Attenuate PD-1 Signaling in T Cells

doi: 10.1016/j.celrep.2018.06.054

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: ZAP70 pY493 antibody , Cell Signaling Technology , Cat # 2704S; RRID: AB_2217457.

Techniques: Recombinant, Enzyme-linked Immunosorbent Assay, Cytotoxicity Assay, Software, Imaging

Journal: iScience

Article Title: Decoding sunitinib resistance in ccRCC: Metabolic-reprogramming-induced ABAT and GABAergic system shifts

doi: 10.1016/j.isci.2024.110415

Figure Lengend Snippet:

Article Snippet: Phospho-SYK (Ser297) , Cell Signaling Technology , Cat# 14140; RRID: AB_2798401.

Techniques: Recombinant, Lysis, Electrophoresis, Cell Counting, Fluorescence, Staining, Phospho-proteomics, Ab Array, Plasmid Preparation, Software