Journal: Histochemistry and Cell Biology

Article Title: Aniridia-related keratopathy relevant cell signaling pathways in human fetal corneas

doi: 10.1007/s00418-022-02099-9

Figure Lengend Snippet: Primary antibodies

Article Snippet: p-rpS6 , GTX 60,800 , 1:800 , Genetex, Irvine, CA, USA.

Techniques: Concentration Assay

Journal: Histochemistry and Cell Biology

Article Title: Aniridia-related keratopathy relevant cell signaling pathways in human fetal corneas

doi: 10.1007/s00418-022-02099-9

Figure Lengend Snippet: Cross-sections of fetal corneas 10–11 wg ( a , e , i , m ) and 20 wg ( b , f , j , n ), adult normal corneas ( c , g , k , o ), and ARK ( d , h , l , p ) labeled with antibodies (green) against Hes1 ( a – d ), Gli1 ( e – h ), mTOR ( i – l ), and p-rpS6 ( m – p ). Tissue preservation of the fetal tissue was variable. Cell nuclei are labeled blue with DAPI ( a – p ). Abs against Hes1 strongly immunolabeled the epithelial cells of the fetal corneas ( a , b ). These Abs labeled streaks in the stroma more profusely in the 10–11 wg ( a ) than in the 20 wg fetal corneas ( b ) and more intensively in the anterior stroma ( b ). In contrast, in the adult corneas, immunolabeling was not detected ( c ), whereas Hes1 was present in the epithelium and in the anterior stroma of the ARK corneas ( d ). Immunolabeling with Abs against Gli1 was present in the epithelial cells in all fetal corneas ( e , f ). Labeling in the stroma was present in streaks in all fetal corneas ( e , f ). Immunolabeling for these Abs was not observed in adult corneas ( g ). In the ARK corneas, the Abs against Gli1 labeled the epithelium and the anterior pannus ( h ). The inserts in a – h show the epithelium at higher magnification. The Ab against mTOR abundantly labeled the epithelial cells of all fetal corneas ( i , j ) but marked the epithelium in the 20 wg fetal corneas more intensively ( j ). The stroma in all fetal corneas was labeled in streaks ( i , j ) but was more abundantly marked in the 10–11 wg fetal corneas ( i ). This Ab did not immunolabel the adult corneas ( k ), but the epithelium and anterior pannus were labeled by this Ab in the ARK corneas ( l ). The Ab against p-rpS6 labeled the epithelial cells and abundant streaks in the stroma of all fetal corneas in a likewise pattern in both 10–11 wg ( m ) and 20 wg fetal corneas ( n ). The stroma in the adult corneas was not labeled and the surface of the epithelium was only scarcely labeled, suggesting sticky adherence to the epithelial surface ( o ). In the ARK corneas, immunostaining against p-rpS6 was present in the epithelium and anterior pannus ( p ). Bars, 100 μm

Article Snippet: p-rpS6 , GTX 60,800 , 1:800 , Genetex, Irvine, CA, USA.

Techniques: Labeling, Preserving, Immunolabeling, Immunostaining

Journal: Histochemistry and Cell Biology

Article Title: Aniridia-related keratopathy relevant cell signaling pathways in human fetal corneas

doi: 10.1007/s00418-022-02099-9

Figure Lengend Snippet: Gene expression of Notch1 ( NOTCH1 ), Dlk1 ( DLK1 ), Numb ( NUMB ) ( a ), Wnt5A ( WNT5A ), Wnt7A ( WNT7A ), β-catenin ( CTNNB1 ) ( b ), and Hes1 ( HES1 ), mTOR ( mTOR ), and rps6 ( RPS6 ) ( c ) in 9–12 wg fetal corneas as compared with adult cornea. There was a 3.89-fold increase in Notch1 gene expression, 1540-fold increase in Dlk1 gene expression, and 0.64-fold decrease in Numb gene expression in 9–12 wg fetal corneas. ( a ). Expression of Wnt5A , Wnt7A , and β-catenin genes was increased by 1.85-, 3.57-, and 2.05-fold, respectively. ( b ). Gene expression of Hes1 , mTOR , and rps6 was increased by 2.73-, 1.89-, and 1.78-fold, respectively ( c ). Values are mean ± SD. * p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001

Article Snippet: p-rpS6 , GTX 60,800 , 1:800 , Genetex, Irvine, CA, USA.

Techniques: Gene Expression, Expressing

Journal: Marine Life Science & Technology

Article Title: General transcription factor IIIAa regulates the transcription of somatic 5S rRNA and influences embryonic organ development in zebrafish

doi: 10.1007/s42995-025-00334-5

Figure Lengend Snippet: gtf3aa mutations disrupt 5S rRNA transcription and ribosome biogenesis. A Expression patterns of somatic 5S rRNA in gtf3aa −/− and gtf3aa + / + larvae from 1–5 dpf. 18S rRNA served as the reference gene in qPCR. B Polysome profiling of gtf3aa −/− and gtf3aa + / + larvae at 3 dpf. C Western blot analysis of Rps6 and Rpl35 protein levels in gtf3aa −/− and gtf3aa + / + larvae at 3 dpf, using β-Actin as a loading control. so_5S rRNA somatic 5S rRNA

Article Snippet: Primary antibodies used included Anti-RPS6 antibody ( HA600084 ; Huabio, Hangzhou, China), Anti-RPL35 antibody (ER64837; Huabio), and Anti-β-Actin antibody (AC026; ABClonal, Hubei, China).

Techniques: Expressing, Western Blot, Control