Journal: Journal of Virology

Article Title: Differential Activation of Human Monocyte-Derived and Plasmacytoid Dendritic Cells by West Nile Virus Generated in Different Host Cells

doi: 10.1128/jvi.00857-07

Figure Lengend Snippet: FIG. 4. Infection with WNV or SeV results in IRF3 translocation in mDC. Micrographs of mDC cultures treated with SeV (16 HAU/ml) (A) or WNV (MOI of 100, based on titration on Vero cells) (B) were exposed for the indicated times and then fixed and subjected to indirect immunofluorescent staining as described in Materials and Methods. Nuclei were stained with DAPI (500 ng/ml); IRF3 was detected with rabbit polyclonal anti-IRF3 decorated with an FITC label, and WNV antigen was detected with anti-WNV MHIAF decorated with a Texas Red label.

Article Snippet: After 1 h, cells were washed two times with PBS and incubated with a fluorescein isothiocyanate (FITC)-labeled goat anti-rabbit antibody (1:100) (Southern Biotechnology, Birmingham, AL) and Texas Red-labeled goat anti-mouse antibody (1:100) (KPL, Gaithersburg, MD) for the dou- ble-labeled WNV-infected samples.

Techniques: Infection, Translocation Assay, Titration, Staining