Journal: Frontiers in Neuroscience

Article Title: Protecting RNA quality for spatial transcriptomics while improving immunofluorescent staining quality

doi: 10.3389/fnins.2023.1198154

Figure Lengend Snippet: Result summary of tested antibodies in our optimized protocol.

Article Snippet: PSD95 , Excitatory postsynapse , ms , StressMarq , SMC-122D , AB_2300386 , 1:500; 2 μg/mL , No.

Techniques: Marker, Concentration Assay

Journal: IBRO Neuroscience Reports

Article Title: IL-33 relieves nerve injury by mediating microglial polarization in neuromyelitis optica spectrum disorders via the IL-33/ST2 pathway

doi: 10.1016/j.ibneur.2024.07.008

Figure Lengend Snippet: Microglial cells were activated and transformed to the M2 phenotype after treatment with IL-33. (a) Western blot images and the quantification of CD206 and CD40 levels after treatment with IL-33 or NMOD serum. (b) BV2 cells were labeled with Iba-1 and CD68 for immunofluorescence. (c) Expression levels of PSD95 were observed by immunofluorescence after treatment with IL-33 or NMOSD serum. One-way analysis of variance was used for statistical analyses. *p<0.05, **p<0.01, ***p<0.001.

Article Snippet: For protein immunoreactivity in cells, the primary cortical neurons or BV2 cells were fixed with 4 % paraformaldehyde for 30 min at RT and treated with 0.5 % Triton for 10 min. After being washed with PBS, the cells were blocked with 5 % bovine serum albumin (BSA) for 1 h and then incubated with a rabbit anti-PSD95 antibody (1:100 dilution, PA2295, Boster), anti-CD68 antibody (1:200 dilution, 28058–1-AP, Proteintech), anti-IBA1 antibody (1:100 dilution, CY7217, Abways), or anti-ST2 antibody (1:200 dilution, PRS3363, Sigma) at 4 °C overnight.

Techniques: Transformation Assay, Western Blot, Labeling, Immunofluorescence, Expressing

Journal: Frontiers in Molecular Neuroscience

Article Title: Behavioral and Molecular Responses to Exogenous Cannabinoids During Pentylenetetrazol-Induced Convulsions in Male and Female Rats

doi: 10.3389/fnmol.2022.868583

Figure Lengend Snippet: Expression levels of proteins obtained from a fraction enriched in cytoplasmic proteins/proteins bound to small organelles. (A) Representative Western blots of CB1R, GluA2R, βArr2, PSD95, p-CaMKII and total CaMKII in females and (I) males. (B–H) Quantification of (A) (Females. CB1R: C, n = 5; PTZ, n = 8; WIN, n = 4; WIN + PTZ, n = 6; GluA2: C, n = 5; PTZ, n = 9; WIN, n = 5; WIN + PTZ, n = 6; βArr2: C, n = 5; PTZ, n = 9; WIN, n = 5; WIN + PTZ, n = 6; PSD95: C, n = 5; PTZ, n = 8; WIN, n = 5; WIN + PTZ, n = 6, p -CaMKIIα: C, n = 5; PTZ, n = 8; WIN, n = 4; WIN + PTZ, n = 5; p -CaMKIIβ: C, n = 5; PTZ, n = 7; WIN, n = 5; WIN + PTZ, n = 5; total CaMKII: C, n = 3; PTZ, n = 4; WIN, n = 3; WIN + PTZ, n = 3). (J–P) Quantification of (D) (Males. CB1R: C, n = 4; PTZ, n = 6; WIN, n = 3; WIN + PTZ, n = 5; GluA2: C, n = 4; PTZ, n = 7; WIN, n = 5; WIN + PTZ, n = 5; βArr2: C, n = 4; PTZ, n = 5; WIN, n = 4; WIN + PTZ, n = 5; PSD95: C, n = 4; PTZ, n = 6; WIN, n = 4; WIN + PTZ, n = 5, p -CaMKIIα: C, n = 4; PTZ, n = 5; WIN, n = 4; WIN + PTZ, n = 5; p -CaMKIIβ: C, n = 4; PTZ, n = 5; WIN, n = 4; WIN + PTZ, n = 5; total CaMKII: C, n = 3; PTZ, n = 4; WIN, n = 3; WIN + PTZ, n = 3). Tubulin was used as protein loading control for all gels. * p < 0.05 and ** p < 0.01, one-way ANOVA with Tukey’s multiple comparisons post hoc .

Article Snippet: Primary antibodies used were anti CB1, 1:1,000 (Ab23703, Abcam, UK), anti βArr2, 1:500 (Ab31294, Abcam), anti phospho T286phosphorylated calcium/calmodulin-dependent protein kinase II (p-CaMKII), 1:2,000 (Ab32678, Abcam), anti postsynaptic density protein 95 (PSD95) 1:1,000 (#610496, BD Biosciences, USA), anti α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid, AMPA, subunit GluA2 (GluA2), 1:1,000 (Sc-517265, Santa Cruz Biotechnology, USA), anti calcium/calmodulin-dependent protein kinase II (total CaMKII) (Sc-5306, Santa Cruz Biotechnology), antiGolgi matrix protein 130 (GM130), 1:1,000 (#610823, BD Biosciences, USA), anti βIII-Tubulin, 1:2,500 (G7121, Promega, USA), anti N-cadherin, 1:1,000 (#33-3900, Invitrogen).

Techniques: Expressing, Western Blot, Control