Journal:

Article Title: Nonpathogenic Escherichia coli Can Contribute to the Production of Shiga Toxin

doi: 10.1128/IAI.71.6.3107-3115.2003

Figure Lengend Snippet: Bacterial strains, plasmids, and primers used in this study

Article Snippet: The primary anti-GFP antibody (BD Biosciences Clontech, Palo Alto, Calif.) was used at a 1:100 dilution, followed by goat anti-rabbit secondary antibody (Cappel, West Chester, Pa.) used at a dilution of 1:37,500.

Techniques: Plasmid Preparation, Sequencing, Transformation Assay, Isolation, Clone Assay, TA Cloning

Journal: Retrovirology

Article Title: Efficient trapping of HIV-1 envelope protein by hetero-oligomerization with an N-helix chimera

doi: 10.1186/1742-4690-2-51

Figure Lengend Snippet: A. Comparison of consensus amino acid sequences of N-helix regions from various HIV-1 clades, and the consensus sequence that was used to make pNH-YFPgpi. The letters a and d under the consensus sequence indicate the position of corresponding amino acids on a helical wheel. B. Schematic diagram of the coding sequence regions in expression plasmids pYFPgpi and pNH-YFPgpi. SP, signal peptide; YFP, yellow fluorescent protein; NH, N-helix; GPI, gpi attachment signal. C. Western blot with anti-GFP antiserum of HeLa cells transfected with pNH-YFPgpi (lane 1), pYFPgpi (lane 2), or untransfected HeLa cells (lane 3). *, aberrant YFPgpi product. One of three independent experiments with similar results is shown.

Article Snippet: The supernatant was collected and immunoprecipitated with monoclonal anti-GFP antibody (Clontech, Palo Alto, CA) overnight and protein G beads for an additional 2 hours.

Techniques: Comparison, Sequencing, Expressing, Western Blot, Transfection

Journal: Retrovirology

Article Title: Efficient trapping of HIV-1 envelope protein by hetero-oligomerization with an N-helix chimera

doi: 10.1186/1742-4690-2-51

Figure Lengend Snippet: Western blot analysis of HeLa cells transfected with an expression vector for HIV-1 Env plus either pNH-YFPgpi (lanes 1–3) or pYFPgpi as control (lanes 4–6). Total cell lysates (lanes 1, 4) or anti-GFP immunoprecipitates (lanes 2, 3, 5, 6) were treated with furin (lanes 3, 6) or mock treated (lanes 1, 2, 4, 5) and analyzed with rabbit anti-gp120 antiserum.

Article Snippet: The supernatant was collected and immunoprecipitated with monoclonal anti-GFP antibody (Clontech, Palo Alto, CA) overnight and protein G beads for an additional 2 hours.

Techniques: Western Blot, Transfection, Expressing, Plasmid Preparation, Control