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Rockland Immunochemicals
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Boster Bio
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Boster Bio
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Boster Bio
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Boster Bio
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GeneTex
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Goat Anti-Human ERN1 / IRE1a, (internal region)
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Image Search Results
Journal: Stem Cells International
Article Title: Mesenchymal Stem Cells Inhibit Epithelial-to-Mesenchymal Transition by Modulating the IRE1 α Branch of the Endoplasmic Reticulum Stress Response
doi: 10.1155/2023/4483776
Figure Lengend Snippet: MSCs attenuated EMT via the IRE1 α /XBP1 pathway. (a) The protein expression levels of IRE1 α and p-IRE1 α were measured using western blotting and quantified using densitometry in ImageJ software ( n = 4, one-way ANOVA with Duncan's post hoc test). (b) The mRNA expression level of IRE1 α was measured using Q-PCR ( n = 3, one-way ANOVA with Duncan's post hoc test). (c) A549 cells were treated with 4 μ 8c (5 and 10 μ M) for 48 hr, and the protein expression levels of IRE1 α and XBP-1s were measured using western blotting and quantified using densitometry in ImageJ software ( n = 4, one-way ANOVA with Duncan's post hoc test). (d) A549 cells were treated with 10 ng/ml TGF- β 1 in the presence or absence of 4 μ 8c for 72 hr. The protein expression levels of p-IRE1 α , XBP-1s, E-cadherin and vimentin were measured using western blotting and quantified using densitometry in ImageJ software ( n = 4, one-way ANOVA with Duncan's post hoc test). The data are shown as the means ± SEMs ( ∗∗∗ P < 0.001, ∗∗ P < 0.01, ∗ P < 0.05 vs. the control group; ### P < 0.001, ## P < 0.01, # P < 0.05 vs. the TGF- β 1 group).
Article Snippet: After the proteins were separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, they were transferred to membranes, which were blocked with Protein Free Rapid Blocking Buffer (PS108, EpiZyme) and incubated with primary antibodies against CHOP (A5462, Bimake), BiP (11587-1-AP; Proteintech, Wuhan, China), ATF6 (D262665, Sangon, China), ATF4 (A5514, Bimake), XBP-1s (24868-1-AP, Proteintech), XBP-1u (25997-1-AP, Proteintech),
Techniques: Expressing, Western Blot, Software, Control
Journal: Stem Cells International
Article Title: Mesenchymal Stem Cells Inhibit Epithelial-to-Mesenchymal Transition by Modulating the IRE1 α Branch of the Endoplasmic Reticulum Stress Response
doi: 10.1155/2023/4483776
Figure Lengend Snippet: MSCs attenuated ER stress and EMT in the lungs of mice with lung fibrosis. (a) The mRNA expression levels of E-cadherin and Vimentin in lung tissues were measured using Q-PCR ( n = 5, one-way ANOVA with Duncan's post hoc test). (b) The protein expression levels of E-cadherin and vimentin in lung tissues were measured using western blotting, and the results were quantified via densitometry by using ImageJ software ( n = 3, one-way ANOVA with Duncan's post hoc test). (c) The mRNA expression levels of Bip , Atf6 , Atf4 , Xbp-1s , Ire1α and Chop in lung tissues were measured using Q-PCR ( n = 5, one-way ANOVA with Duncan's post hoc test). (d) The protein expression levels of ATF6, ATF4, IRE1 α , p-IRE1 α , XBP-1s, XBP-1u, BiP and CHOP in lung tissues were measured using western blotting, and the results were quantified via densitometry by using ImageJ software ( n = 3, one-way ANOVA with Duncan's post hoc test). (e) Images of immunofluorescence staining of BiP (green) and vimentin (red) in the lung tissues of mice. Scale bar, 100 μ m. The data are shown as the means ± SEMs ( ∗∗ P < 0.01, ∗ P < 0.05 vs. the control group; # P < 0.05, ## P < 0.01 vs. the BLM group).
Article Snippet: After the proteins were separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, they were transferred to membranes, which were blocked with Protein Free Rapid Blocking Buffer (PS108, EpiZyme) and incubated with primary antibodies against CHOP (A5462, Bimake), BiP (11587-1-AP; Proteintech, Wuhan, China), ATF6 (D262665, Sangon, China), ATF4 (A5514, Bimake), XBP-1s (24868-1-AP, Proteintech), XBP-1u (25997-1-AP, Proteintech),
Techniques: Expressing, Western Blot, Software, Immunofluorescence, Staining, Control