anti il8 Search Results


93
Alomone Labs chemokine
Chemokine, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Miltenyi Biotec cxcl8
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Boster Bio interleukin 8
Interleukin 8, supplied by Boster Bio, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Boster Bio immunohistochemistry staining
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Boster Bio cxcr1
FIGURE 2 Expression changes of <t>CXCL8-CXCR1/2</t> in UC tissue versus normal colon tissue. (A) Multiplex fluorescent immunohistochemistry was used to measure CXCL8, CXCR1, and CXCR2 protein levels in normal colon tissue (NCT) and ulcerative colitis tissues (UCT). CXCL8 was labeled in green, CXCR1 was labeled in red, CXCR2 was labeled purple, and DAPI was used to label nuclei in blue (magnification ×200); (B–D) Changes in AOD (average optical density) protein expression levels of CXCL8-CXCR1/2 in different intestinal tissues (n = 5). *p < 0.05, **p < 0.01, ***p < 0.001 vs. NCT.
Cxcr1, supplied by Boster Bio, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
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91
Kingfisher Biotech biotinylated anti bovine il 8
FIGURE 2 Expression changes of <t>CXCL8-CXCR1/2</t> in UC tissue versus normal colon tissue. (A) Multiplex fluorescent immunohistochemistry was used to measure CXCL8, CXCR1, and CXCR2 protein levels in normal colon tissue (NCT) and ulcerative colitis tissues (UCT). CXCL8 was labeled in green, CXCR1 was labeled in red, CXCR2 was labeled purple, and DAPI was used to label nuclei in blue (magnification ×200); (B–D) Changes in AOD (average optical density) protein expression levels of CXCL8-CXCR1/2 in different intestinal tissues (n = 5). *p < 0.05, **p < 0.01, ***p < 0.001 vs. NCT.
Biotinylated Anti Bovine Il 8, supplied by Kingfisher Biotech, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Kingfisher Biotech rabbit anti bovine il 8 polyclonal antibody 185
FIGURE 2 Expression changes of <t>CXCL8-CXCR1/2</t> in UC tissue versus normal colon tissue. (A) Multiplex fluorescent immunohistochemistry was used to measure CXCL8, CXCR1, and CXCR2 protein levels in normal colon tissue (NCT) and ulcerative colitis tissues (UCT). CXCL8 was labeled in green, CXCR1 was labeled in red, CXCR2 was labeled purple, and DAPI was used to label nuclei in blue (magnification ×200); (B–D) Changes in AOD (average optical density) protein expression levels of CXCL8-CXCR1/2 in different intestinal tissues (n = 5). *p < 0.05, **p < 0.01, ***p < 0.001 vs. NCT.
Rabbit Anti Bovine Il 8 Polyclonal Antibody 185, supplied by Kingfisher Biotech, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Boster Bio rabbit anti cxcr2
FIGURE 2 Expression changes of <t>CXCL8-CXCR1/2</t> in UC tissue versus normal colon tissue. (A) Multiplex fluorescent immunohistochemistry was used to measure CXCL8, CXCR1, and CXCR2 protein levels in normal colon tissue (NCT) and ulcerative colitis tissues (UCT). CXCL8 was labeled in green, CXCR1 was labeled in red, CXCR2 was labeled purple, and DAPI was used to label nuclei in blue (magnification ×200); (B–D) Changes in AOD (average optical density) protein expression levels of CXCL8-CXCR1/2 in different intestinal tissues (n = 5). *p < 0.05, **p < 0.01, ***p < 0.001 vs. NCT.
Rabbit Anti Cxcr2, supplied by Boster Bio, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Miltenyi Biotec anti huil 8 apc
FIGURE 2 Expression changes of <t>CXCL8-CXCR1/2</t> in UC tissue versus normal colon tissue. (A) Multiplex fluorescent immunohistochemistry was used to measure CXCL8, CXCR1, and CXCR2 protein levels in normal colon tissue (NCT) and ulcerative colitis tissues (UCT). CXCL8 was labeled in green, CXCR1 was labeled in red, CXCR2 was labeled purple, and DAPI was used to label nuclei in blue (magnification ×200); (B–D) Changes in AOD (average optical density) protein expression levels of CXCL8-CXCR1/2 in different intestinal tissues (n = 5). *p < 0.05, **p < 0.01, ***p < 0.001 vs. NCT.
Anti Huil 8 Apc, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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93
Bio-Techne corporation ab against cxcl8
FIGURE 2 Expression changes of <t>CXCL8-CXCR1/2</t> in UC tissue versus normal colon tissue. (A) Multiplex fluorescent immunohistochemistry was used to measure CXCL8, CXCR1, and CXCR2 protein levels in normal colon tissue (NCT) and ulcerative colitis tissues (UCT). CXCL8 was labeled in green, CXCR1 was labeled in red, CXCR2 was labeled purple, and DAPI was used to label nuclei in blue (magnification ×200); (B–D) Changes in AOD (average optical density) protein expression levels of CXCL8-CXCR1/2 in different intestinal tissues (n = 5). *p < 0.05, **p < 0.01, ***p < 0.001 vs. NCT.
Ab Against Cxcl8, supplied by Bio-Techne corporation, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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86
Thermo Fisher gene exp il8 ss03392437 m1
FIGURE 2 Expression changes of <t>CXCL8-CXCR1/2</t> in UC tissue versus normal colon tissue. (A) Multiplex fluorescent immunohistochemistry was used to measure CXCL8, CXCR1, and CXCR2 protein levels in normal colon tissue (NCT) and ulcerative colitis tissues (UCT). CXCL8 was labeled in green, CXCR1 was labeled in red, CXCR2 was labeled purple, and DAPI was used to label nuclei in blue (magnification ×200); (B–D) Changes in AOD (average optical density) protein expression levels of CXCL8-CXCR1/2 in different intestinal tissues (n = 5). *p < 0.05, **p < 0.01, ***p < 0.001 vs. NCT.
Gene Exp Il8 Ss03392437 M1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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89
Boster Bio il 8
FIGURE 2 Expression changes of <t>CXCL8-CXCR1/2</t> in UC tissue versus normal colon tissue. (A) Multiplex fluorescent immunohistochemistry was used to measure CXCL8, CXCR1, and CXCR2 protein levels in normal colon tissue (NCT) and ulcerative colitis tissues (UCT). CXCL8 was labeled in green, CXCR1 was labeled in red, CXCR2 was labeled purple, and DAPI was used to label nuclei in blue (magnification ×200); (B–D) Changes in AOD (average optical density) protein expression levels of CXCL8-CXCR1/2 in different intestinal tissues (n = 5). *p < 0.05, **p < 0.01, ***p < 0.001 vs. NCT.
Il 8, supplied by Boster Bio, used in various techniques. Bioz Stars score: 89/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


FIGURE 2 Expression changes of CXCL8-CXCR1/2 in UC tissue versus normal colon tissue. (A) Multiplex fluorescent immunohistochemistry was used to measure CXCL8, CXCR1, and CXCR2 protein levels in normal colon tissue (NCT) and ulcerative colitis tissues (UCT). CXCL8 was labeled in green, CXCR1 was labeled in red, CXCR2 was labeled purple, and DAPI was used to label nuclei in blue (magnification ×200); (B–D) Changes in AOD (average optical density) protein expression levels of CXCL8-CXCR1/2 in different intestinal tissues (n = 5). *p < 0.05, **p < 0.01, ***p < 0.001 vs. NCT.

Journal: Frontiers in Pharmacology

Article Title: Quercetin alleviates ulcerative colitis through inhibiting CXCL8-CXCR1/2 axis: a network and transcriptome analysis

doi: 10.3389/fphar.2024.1485255

Figure Lengend Snippet: FIGURE 2 Expression changes of CXCL8-CXCR1/2 in UC tissue versus normal colon tissue. (A) Multiplex fluorescent immunohistochemistry was used to measure CXCL8, CXCR1, and CXCR2 protein levels in normal colon tissue (NCT) and ulcerative colitis tissues (UCT). CXCL8 was labeled in green, CXCR1 was labeled in red, CXCR2 was labeled purple, and DAPI was used to label nuclei in blue (magnification ×200); (B–D) Changes in AOD (average optical density) protein expression levels of CXCL8-CXCR1/2 in different intestinal tissues (n = 5). *p < 0.05, **p < 0.01, ***p < 0.001 vs. NCT.

Article Snippet: The samples were then separated by SDS-PAGE, transferred to a PVDFmembrane, blocked with 5% BSA, and incubated with a primary antibody at 4°C for 14 h (CXCL8, Proteintech, diluted 1:1,000; CXCR1, Affinity, diluted 1: 1,000; CXCR2, Proteintech, diluted 1:1,000; IL-17A, BOSTER, diluted 1:1,000; NF-κB, Affinity, diluted 1:1,000; ZO-1, Affinity, diluted 1:2,000; MUC2, Abcam, diluted 1:2,000; Occludin, Affinity, diluted 1:1,000; GAPDH, Servicebio, diluted 1:4,000).

Techniques: Expressing, Multiplex Assay, Immunohistochemistry, Labeling

FIGURE 3 CXCL8-CXCR1/2 gene expression in the transcriptome analysis. (A) KEGG pathway enrichment reveals the UC’s top 20 significantly enriched pathways group. (B–D) GSEA results compare the UC group to the HC group, using the Normalized Enrichment Score (NES) as a key metric. NES >0 indicates core genes on the left of the peak, while NES <0 indicates core genes on the right. Significance of the Enrichment Score (ES) is assessed using the p-value.

Journal: Frontiers in Pharmacology

Article Title: Quercetin alleviates ulcerative colitis through inhibiting CXCL8-CXCR1/2 axis: a network and transcriptome analysis

doi: 10.3389/fphar.2024.1485255

Figure Lengend Snippet: FIGURE 3 CXCL8-CXCR1/2 gene expression in the transcriptome analysis. (A) KEGG pathway enrichment reveals the UC’s top 20 significantly enriched pathways group. (B–D) GSEA results compare the UC group to the HC group, using the Normalized Enrichment Score (NES) as a key metric. NES >0 indicates core genes on the left of the peak, while NES <0 indicates core genes on the right. Significance of the Enrichment Score (ES) is assessed using the p-value.

Article Snippet: The samples were then separated by SDS-PAGE, transferred to a PVDFmembrane, blocked with 5% BSA, and incubated with a primary antibody at 4°C for 14 h (CXCL8, Proteintech, diluted 1:1,000; CXCR1, Affinity, diluted 1: 1,000; CXCR2, Proteintech, diluted 1:1,000; IL-17A, BOSTER, diluted 1:1,000; NF-κB, Affinity, diluted 1:1,000; ZO-1, Affinity, diluted 1:2,000; MUC2, Abcam, diluted 1:2,000; Occludin, Affinity, diluted 1:1,000; GAPDH, Servicebio, diluted 1:4,000).

Techniques: Gene Expression

FIGURE 6 Que regulated the CXCL8-CXCR1/2 axis. (A–D) CXCL8, CXCR1, and CXCR2 expression levels in mice’s colonic tissue (n = 4). (E–H) IHC expression of CXCL8, CXCR1, and CXCR2 in the colon (×100, ×400) (n = 4).*p < 0.05, **p < 0.01, ***p < 0.001.

Journal: Frontiers in Pharmacology

Article Title: Quercetin alleviates ulcerative colitis through inhibiting CXCL8-CXCR1/2 axis: a network and transcriptome analysis

doi: 10.3389/fphar.2024.1485255

Figure Lengend Snippet: FIGURE 6 Que regulated the CXCL8-CXCR1/2 axis. (A–D) CXCL8, CXCR1, and CXCR2 expression levels in mice’s colonic tissue (n = 4). (E–H) IHC expression of CXCL8, CXCR1, and CXCR2 in the colon (×100, ×400) (n = 4).*p < 0.05, **p < 0.01, ***p < 0.001.

Article Snippet: The samples were then separated by SDS-PAGE, transferred to a PVDFmembrane, blocked with 5% BSA, and incubated with a primary antibody at 4°C for 14 h (CXCL8, Proteintech, diluted 1:1,000; CXCR1, Affinity, diluted 1: 1,000; CXCR2, Proteintech, diluted 1:1,000; IL-17A, BOSTER, diluted 1:1,000; NF-κB, Affinity, diluted 1:1,000; ZO-1, Affinity, diluted 1:2,000; MUC2, Abcam, diluted 1:2,000; Occludin, Affinity, diluted 1:1,000; GAPDH, Servicebio, diluted 1:4,000).

Techniques: Expressing

FIGURE 8 Workflow for analyzing Que’s mechanisms in UC treatment. Network pharmacology, in conjunction with transcriptomic methodologies and experimental validation, demonstrated that quercetin alleviated the symptoms associated with DSS-induced colitis. It attenuates the inflammatory response, promotes the repair of colonic mucosal tissues, and elevates the levels of colonic tight junction proteins in mice with ulcerative colitis. These effects may enhance tight junction integrity and restore intestinal barrier function by modulating the IL-17A signaling pathway while simultaneously inhibiting the CXCL8-CXCR1/2 axis.

Journal: Frontiers in Pharmacology

Article Title: Quercetin alleviates ulcerative colitis through inhibiting CXCL8-CXCR1/2 axis: a network and transcriptome analysis

doi: 10.3389/fphar.2024.1485255

Figure Lengend Snippet: FIGURE 8 Workflow for analyzing Que’s mechanisms in UC treatment. Network pharmacology, in conjunction with transcriptomic methodologies and experimental validation, demonstrated that quercetin alleviated the symptoms associated with DSS-induced colitis. It attenuates the inflammatory response, promotes the repair of colonic mucosal tissues, and elevates the levels of colonic tight junction proteins in mice with ulcerative colitis. These effects may enhance tight junction integrity and restore intestinal barrier function by modulating the IL-17A signaling pathway while simultaneously inhibiting the CXCL8-CXCR1/2 axis.

Article Snippet: The samples were then separated by SDS-PAGE, transferred to a PVDFmembrane, blocked with 5% BSA, and incubated with a primary antibody at 4°C for 14 h (CXCL8, Proteintech, diluted 1:1,000; CXCR1, Affinity, diluted 1: 1,000; CXCR2, Proteintech, diluted 1:1,000; IL-17A, BOSTER, diluted 1:1,000; NF-κB, Affinity, diluted 1:1,000; ZO-1, Affinity, diluted 1:2,000; MUC2, Abcam, diluted 1:2,000; Occludin, Affinity, diluted 1:1,000; GAPDH, Servicebio, diluted 1:4,000).

Techniques: Biomarker Discovery