Journal: Cell reports. Medicine

Article Title: Tissue-resident memory CAR T cells with stem-like characteristics display enhanced efficacy against solid and liquid tumors.

doi: 10.1016/j.xcrm.2023.101053

Figure Lengend Snippet: Figure 2. CAR-TRM cells possess a core resident memory transcriptomic signature Bulk RNA-seq experiments were conducted using CD8+ CAR-TCONV and CAR-TRM cells. (A) Principal-component analysis was carried out with RNA expression data. (B) Gene set enrichment analysis (GSEA) using gene sets associated with resident memory T cells. (C) Heatmap showing differentially expressed genes from CD69+CD103+ and CD69CD103CD8+ T cell signatures. (D) Heatmap displaying expression of transcription factor genes known to promote and suppress resident memory T cell differentiation. (E) GSEA (hypoxia pathways: MSigDB C2). (F) Pathway analysis incorporating genes upregulated in CAR-TRM-cells using Enrichr. (G) GSEA analysis (migration pathways: MSigDB C2). GSEA with gene signatures of tumor-infiltrating T cells associated with response to ICB. Bulk RNA-seq experiments were conducted using CAR T cells derived from n = 3 distinct healthy donors, serving as biological replicates.

Article Snippet: To examine the role of the resident memory population in mediating CAR-TRM cell antitumor responses, the CD103 + population in CAR-TRM products was magnetically depleted using biotinylated anti-human CD103 antibody and anti-biotin microbeads (Miltenyi Biotec).

Techniques: RNA Sequencing, RNA Expression, Expressing, Cell Differentiation, Migration, Derivative Assay

Journal: Cell reports. Medicine

Article Title: Tissue-resident memory CAR T cells with stem-like characteristics display enhanced efficacy against solid and liquid tumors.

doi: 10.1016/j.xcrm.2023.101053

Figure Lengend Snippet: Figure 3. TGF-b-generated CAR-TRM cells transcriptionally resemble stem-like TRM cells (A) UMAP imbedding of CD8+CAR T cell (top, CD8 subclusters; middle, CAR T cell samples; bottom, cell cycle). Frequencies of three CD8+ clusters in CAR-TCONV and CAR-TRM cells are shown. (B) Bubble plot displaying expression levels of marker genes in each CD8+ subcluster. (C) Violin plots showing expression of KLF2 and ITGAE.

Article Snippet: To examine the role of the resident memory population in mediating CAR-TRM cell antitumor responses, the CD103 + population in CAR-TRM products was magnetically depleted using biotinylated anti-human CD103 antibody and anti-biotin microbeads (Miltenyi Biotec).

Techniques: Generated, Expressing, Marker

Journal: Cell reports. Medicine

Article Title: Tissue-resident memory CAR T cells with stem-like characteristics display enhanced efficacy against solid and liquid tumors.

doi: 10.1016/j.xcrm.2023.101053

Figure Lengend Snippet: Figure 7. Comparison of CAR-TRM cell differentiation methods (A) RUNX3 expression measured in various anti-mesothelin SS1 CAR T cell products: standard T cell medium culture (control CAR T), standard T cell medium supplemented with TGF-b (TGF-b CAR T) and engineered to overexpress RUNX3 (RUNX3 OE CAR T) (MFI, geometric mean fluorescence intensity). (B) TRM (CD103, CD39, CD49a) and activation (CD25) markers were assessed after CAR T cell manufacturing. (C) NSG mice (n = 7 biological replicates per group) were injected with the EMMESO cell line. Once tumors reached a size of 120 mm3, mice received a single dose of 1 3 107 SS1 CAR T cells. Activated non-transduced T cells served as a negative control. *p < 0.05; **p < 0.01; ***p < 0.001; ns, not significant (one-way ANOVA).

Article Snippet: To examine the role of the resident memory population in mediating CAR-TRM cell antitumor responses, the CD103 + population in CAR-TRM products was magnetically depleted using biotinylated anti-human CD103 antibody and anti-biotin microbeads (Miltenyi Biotec).

Techniques: Comparison, Cell Differentiation, Expressing, Control, Activation Assay, Injection, Negative Control

Journal: iScience

Article Title: Identification of CX3CR1 + mononuclear phagocyte subsets involved in HIV-1 and SIV colorectal transmission

doi: 10.1016/j.isci.2022.104346

Figure Lengend Snippet:

Article Snippet: CD103 , AbDSerotec , Cat# MCA708T; RRID: AB_1100549.

Techniques: Quantitative RT-PCR, Enzyme-linked Immunosorbent Assay, Software

Journal: Cell reports

Article Title: High-dimensional profiling clusters asthma severity by lymphoid and non-lymphoid status

doi: 10.1016/j.celrep.2021.108974

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Anti-Human CD103 (Ber-ACT8)-151Eu , Fluidigm , Cat# 3151011B; RRID:AB_2756418.

Techniques: Purification, Antibody Labeling, Flow Cytometry, Software, Staining, Blocking Assay

Journal: Frontiers in Immunology

Article Title: Cytotoxic innate intraepithelial lymphocytes control early stages of Cryptosporidium infection

doi: 10.3389/fimmu.2023.1229406

Figure Lengend Snippet: Antibodies used for flow cytometry.

Article Snippet: Sony , 1207125 , Pe/Cy7 anti-mouse CD103 , 2E7.

Techniques: Cytometry