Journal: Scientific Reports

Article Title: Single-cell RNA sequencing analysis revealed the immunosuppressive remodeling of tumor-associated macrophages mediated by the MIF-CD74 axis in gastric cancer

doi: 10.1038/s41598-025-10301-w

Figure Lengend Snippet: Expression of MIF and CD74 in gastric cancer. ( A ) Expression of MIF mRNA in GEPIA and TCGA-GC databases. ( B–D ) mRNA levels of MIF in the GSE54129 、GSE65801and GSE29272 dataset from the GEO database. ( E , F ) Kaplan-Meier survival analysis of GC patients based on MIF expression levels. ( G ) Representative IHC images of MIF protein expression in 71 paired GC tumor tissues and adjacent nontumor tissues. Statistical analysis of immunohistochemical score of MIF expression in GC tumor tissues and paired adjacent tissues is shown on the right. ( H ) Expression of CD74 in gastric cancer. ( I ) Representative immunofluorescence staining of CD74 (red), CD68 (green), and nuclei (blue) in paired GC samples.* P < 0.05, ** P < 0.01, *** P < 0.001.

Article Snippet: We used the following antibodies: CD74 (Santa Cruz, ln2), MIF (Proteintech, 20415-1-AP), Goat anti-rabbit antibody (Servicebio, G1213) and goat anti-mouse antibody (Servicebio, G1214).

Techniques: Expressing, Immunohistochemical staining, Immunofluorescence, Staining

Journal: Scientific Reports

Article Title: Single-cell RNA sequencing analysis revealed the immunosuppressive remodeling of tumor-associated macrophages mediated by the MIF-CD74 axis in gastric cancer

doi: 10.1038/s41598-025-10301-w

Figure Lengend Snippet: The MIF-CD74 axis remodeled the TME. ( A ) Expression of MIF in normal gastric epithelial cells and 4 different GC cell lines by Western blot. ( B ) Western blot assays were performed to verify the successful construction of MIF overexpression and knockdown GC cells. ( C ) Colony formation assays were performed to determine the effects of MIF on the growth of GC cells. The number of colonies (> 50 cells) was scored (mean ± SD, n = 3). ( D ) CCK-8 assays were performed to determine the effects of MIF on the proliferation of GC cells (mean ± SD, n = 3). ( E ) Control and MIF overexpressed HGC27 cells formed subcutaneous tumors in nude mice. The image on the right shows the weight of the subcutaneous tumors. ( F ) Control and MIF silenced AGS cells formed tumors subcutaneously in nude mice. The image on the right shows the weight of the subcutaneous tumors. ( G ) Representative immunofluorescence staining of CD74 (red), CD68 (green) and nucleus (blue) in subcutaneous tumor tissue sections. The numbers of CD74 + CD68 + cells per FOV are represented. ( H ) The effect of MIF on macrophages was analyzed by qRT-PCR. Differences were analyzed using the unpaired test two-sided t test ( n = 3). ( I ) Transwell was used to analyze MIF and its influence on macrophage migration. Differences were analyzed using the unpaired test two-sided t test ( n = 3). * P < 0.05, ** P < 0.01, *** P < 0.001, ns indicates P > 0.05.

Article Snippet: We used the following antibodies: CD74 (Santa Cruz, ln2), MIF (Proteintech, 20415-1-AP), Goat anti-rabbit antibody (Servicebio, G1213) and goat anti-mouse antibody (Servicebio, G1214).

Techniques: Expressing, Western Blot, Over Expression, Knockdown, CCK-8 Assay, Control, Immunofluorescence, Staining, Quantitative RT-PCR, Migration

Journal: Cancer Medicine

Article Title: MT3‐MMP down‐regulation promotes tumorigenesis and correlates to poor prognosis in esophageal squamous cell carcinoma

doi: 10.1002/cam4.790

Figure Lengend Snippet: Relation between MT3‐MMP expression and clinicopathologic characteristics in esophageal squamous cell carcinoma (ESCC) ( n = 86)

Article Snippet: Anti‐ MT3‐MMP antibody (ZA‐0266, 1:100, BA1280,Boster, Wuhan, China) was used as primary antibody, with which tissue sections were incubated overnight at 4°.

Techniques: Expressing, Biomarker Discovery

Journal: Pharmaceutics

Article Title: Therapeutic Options for Recurrent Glioblastoma—Efficacy of Talaporfin Sodium Mediated Photodynamic Therapy

doi: 10.3390/pharmaceutics14020353

Figure Lengend Snippet: Patient demographics and characteristics.

Article Snippet: The methylation status of O-6-methylguanine-DNA methyltransferase (MGMT) was not evaluated, but alternatively, the expression of MGMT protein was determined by conducting immunohistochemistry with anti-MGMT monoclonal antibody (MAB16200, Merck, Darmstadt, Germany).

Techniques: Control, Histopathology, Mutagenesis, Expressing

Journal:

Article Title: MT2-MMP-dependent release of collagen IV NC1 domains regulates submandibular gland branching morphogenesis

doi: 10.1016/j.devcel.2009.07.016

Figure Lengend Snippet: (A) qPCR analysis of SMG gene expression at distinct developmental stages shows that MT1, MT2, and MT3 increase at E13 when branching morphogenesis begins.

Article Snippet: Primary antibodies included rabbit anti-mouse MT1, MT2, and MT3 (Triple Point Biologics, OR), rat anti-mouse perlecan, goat or rabbit anti-mouse collagen IV (both Chemicon, CA), and rat anti-mouse syndecan 1 (BD Pharmingen, CA).

Techniques: Expressing

Journal:

Article Title: MT2-MMP-dependent release of collagen IV NC1 domains regulates submandibular gland branching morphogenesis

doi: 10.1016/j.devcel.2009.07.016

Figure Lengend Snippet: (A) SMGs were cultured for 36 h with non-silencing (NS) and MT1-, MT2-, and MT3-siRNAs. MT2-siRNA has the most significant effect on epithelial morphogenesis.

Article Snippet: Primary antibodies included rabbit anti-mouse MT1, MT2, and MT3 (Triple Point Biologics, OR), rat anti-mouse perlecan, goat or rabbit anti-mouse collagen IV (both Chemicon, CA), and rat anti-mouse syndecan 1 (BD Pharmingen, CA).

Techniques: Cell Culture

Journal:

Article Title: MT2-MMP-dependent release of collagen IV NC1 domains regulates submandibular gland branching morphogenesis

doi: 10.1016/j.devcel.2009.07.016

Figure Lengend Snippet: (A) Proliferation decreases after MT2-siRNA treatment. The whole mount Ki67 immunostaining in the left hand panels are a single projection. MT2-siRNA increases intracellular collagen IV staining in the epithelium (E, arrows) and mesenchyme (M, arrowheads) in the middle panels. Images are single 2 µM confocal sections.

Article Snippet: Primary antibodies included rabbit anti-mouse MT1, MT2, and MT3 (Triple Point Biologics, OR), rat anti-mouse perlecan, goat or rabbit anti-mouse collagen IV (both Chemicon, CA), and rat anti-mouse syndecan 1 (BD Pharmingen, CA).

Techniques: Immunostaining, Staining

Journal:

Article Title: MT2-MMP-dependent release of collagen IV NC1 domains regulates submandibular gland branching morphogenesis

doi: 10.1016/j.devcel.2009.07.016

Figure Lengend Snippet: (A) SMGs were cultured with MT1- or MT2-siRNAs and NC1 domains for 36 hr. The number of end buds is in the upper graph, and qPCR analysis of MT2 expression is in the lower graph. The reduction in MT2 expression is restored by NC1 domains.

Article Snippet: Primary antibodies included rabbit anti-mouse MT1, MT2, and MT3 (Triple Point Biologics, OR), rat anti-mouse perlecan, goat or rabbit anti-mouse collagen IV (both Chemicon, CA), and rat anti-mouse syndecan 1 (BD Pharmingen, CA).

Techniques: Cell Culture, Expressing

Journal:

Article Title: MT2-MMP-dependent release of collagen IV NC1 domains regulates submandibular gland branching morphogenesis

doi: 10.1016/j.devcel.2009.07.016

Figure Lengend Snippet: (A) SMGs cultured with MT1- or MT2-siRNAs, and HB-EGF (10 ng/ml) or recMT2 (0.05 µg/mL) for 36 h.

Article Snippet: Primary antibodies included rabbit anti-mouse MT1, MT2, and MT3 (Triple Point Biologics, OR), rat anti-mouse perlecan, goat or rabbit anti-mouse collagen IV (both Chemicon, CA), and rat anti-mouse syndecan 1 (BD Pharmingen, CA).

Techniques: Cell Culture

Journal:

Article Title: MT2-MMP-dependent release of collagen IV NC1 domains regulates submandibular gland branching morphogenesis

doi: 10.1016/j.devcel.2009.07.016

Figure Lengend Snippet: Working model showing how MT2-dependent release of bioactive collagen IV NC1 domains regulates protease expression and proliferation during SMG branching morphogenesis. Epithelial MT2-mediated proteolysis of collagen IV releases NC1 domains that signal via β1 integrins and downstream AKT activation to increase MT2 and Col4a2, as well as epithelial proliferation via both FGFR and HBEGF-mediated mechanisms. HBEGF further increases MT2 expression and upregulates endogenous Hbegf, providing further stimulus for rapid SMG branching morphogenesis.

Article Snippet: Primary antibodies included rabbit anti-mouse MT1, MT2, and MT3 (Triple Point Biologics, OR), rat anti-mouse perlecan, goat or rabbit anti-mouse collagen IV (both Chemicon, CA), and rat anti-mouse syndecan 1 (BD Pharmingen, CA).

Techniques: Expressing, Activation Assay