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Image Search Results
Journal: bioRxiv
Article Title: Melanocortin-4 receptor antagonist TCMCB07 alleviates chemotherapy-induced anorexia and weight loss
doi: 10.1101/2024.09.14.613069
Figure Lengend Snippet: (A - E) SD male rat serum GDF15 concentrations measured from experiments: Cisplatin/TCMCB07 ( A ), 5-Fluorouracil /TCMCB07 ( B ), Cyclophosphamide/TCMCB07 ( C ), Vincristine/TCMCB07 ( D ), and Doxorubicin/TCMCB07 ( E ). ( F ) Schematic of experimental design. All SD male rats were treated with cisplatin chemotherapy via IP injection at a dose of 5.0 mg/kg (1 st cycle) or 3.0 mg/kg (2 nd and 3 rd cycles) once per week for three cycles. All the rats received SC injections twice (2x) daily with either saline or TCMCB07 (3 mg/kg/day) from day 0 to 21. Additionally, all the rats received SC injections twice (2x) weekly with either IgG or GDF15 antibody from day 0 to 21. The dose of TCMCB07 was 3 mg/kg/day, and the dose of GDF15 antibody was 10 mg/kg. Initial and terminal body composition was measured using MRI before and after treatments. Food intake and body weight were monitored daily throughout entire experimental period (day 0-21). At the end of the experiment, tissues were harvested following euthanasia. ( G ) Daily food intake, ( H ) Cumulative food intake, ( I ) Weekly food intake, ( J ) Total food intake after treatment of cisplatin and TCMCB07 in combination with GDF15 antibody. All data in ( A - E ), ( I ), and ( J ) are expressed with each dot representing one sample, and all data in ( G ) and ( H ) are expressed as mean ± SEM for each group. ( A ) and ( B ), n = 11-12. ( C - E ), n = 5-6, as blood samples were collected from half of the animals in these experiments. ( G - J ), n = 10-11. ( G ) and ( H ), *: Cisplatin/saline+IgG vs Cisplatin/saline+GDF15 antibody, #: Cisplatin/saline+IgG vs Cisplatin/TCMCB07+GDF15 antibody, &: Cisplatin/saline+GDF15 antibody vs Cisplatin/TCMCB07+GDF15 antibody. *, #, &, P < 0.05; **, ##, &&, P < 0.01; ***, ###, &&&, P < 0.001; ****, ####, &&&&, P < 0.0001. One-way ANOVA ( A - E ), ( I ), and ( J ). Two-way ANOVA
Article Snippet:
Techniques: Injection, Saline
Journal: bioRxiv
Article Title: Melanocortin-4 receptor antagonist TCMCB07 alleviates chemotherapy-induced anorexia and weight loss
doi: 10.1101/2024.09.14.613069
Figure Lengend Snippet: ( A ) Daily body weight gain (% initial), ( B ) Weekly body weight gain (% initial), ( C ) Fat mass gain (% initial), ( D ) Lean mass gain (% initial), ( E ) Heart mass (% initial body weight), ( F ) Gastrocnemius mass (% initial body weight) after treatment of cisplatin+saline+IgG, cisplatin+saline+GDF15 antibody (Ab), or cisplatin+TCMCB07+GDF15 Ab. All data in ( A ) are expressed as mean ± SEM for each group, and all data in ( B - F ) are expressed with each dot representing one sample. n = 10-11. ( A ), *: Cisplatin/saline+IgG vs Cisplatin/saline+GDF15 antibody, #: Cisplatin/saline+IgG vs Cisplatin/TCMCB07+GDF15 antibody, &: Cisplatin/saline+GDF15 antibody vs Cisplatin/TCMCB07+GDF15 antibody. * P < 0.05; **, ##, &&, P < 0.01; ***, P < 0.001; ****, ####, P < 0.0001. Two-way ANOVA ( A ), One-way ANOVA ( B - F ).
Article Snippet:
Techniques: Saline