Journal: The Journal of urology

Article Title: A Murine Model of K-RAS and β-Catenin Induced Renal Tumors Expresses High Levels of E2F1 and Resembles Human Wilms Tumor.

doi: 10.1016/j.juro.2015.04.090

Figure Lengend Snippet: Figure 5. E2F1 is up-regulated in murine KrasG12D/CatnbDex3 kidneys. Mice were bred and sacrificed at ages 15 to 20 weeks. Kidneys were snap frozen, RNA was extracted and RT-PCR was performed for E2F1 (A). Results were compared to kidneys from controls and analyzed across groups using 2eDDC T .16 BCat, b-catenin. Asterisk indicates p <0.05. Whole protein lysates were extracted and tested by immunoblot (B). Ctrl, control. Formalin fixed, paraffin embedded kidneys were tested for E2F1 expression by IHC (C to F ). Reduced from 20 (C to F ).

Article Snippet: The antibodies used for IHC and/or immunoblotting were c-Myc (Epitomics ), Actin (Sigma-Aldrich ), Axin2 (Abcam ), E2F1 (Atlas Antibodies, Stockholm, Sweden), survivin and Cyclin D1 (Cell Signaling Technology ).

Techniques: Reverse Transcription Polymerase Chain Reaction, Western Blot, Control, Expressing

Journal: The Journal of urology

Article Title: A Murine Model of K-RAS and β-Catenin Induced Renal Tumors Expresses High Levels of E2F1 and Resembles Human Wilms Tumor.

doi: 10.1016/j.juro.2015.04.090

Figure Lengend Snippet: Figure 6. E2F1 is expressed at high levels in most human WTs. IHC was performed for E2F1 on TMA of 32 human WTs. Strong nuclear staining was seen in 30 of 32 WTs (94%) and seen in blastemal elements at low power (A) and high power (B), in stromal elements (asterisk, B), and epithelial elements at low power (C ) and high power (D). Reduced from 20 (A and C ) and 40 (B and D).

Article Snippet: The antibodies used for IHC and/or immunoblotting were c-Myc (Epitomics ), Actin (Sigma-Aldrich ), Axin2 (Abcam ), E2F1 (Atlas Antibodies, Stockholm, Sweden), survivin and Cyclin D1 (Cell Signaling Technology ).

Techniques: Staining

Journal: Frontiers in Cell and Developmental Biology

Article Title: Safrana l Prevents Prostate Cancer Recurrence by Blocking the Re-activation of Quiescent Cancer Cells via Downregulation of S-Phase Kinase-Associated Protein 2

doi: 10.3389/fcell.2020.598620

Figure Lengend Snippet: The inhibitory effects of safranal on AKT phosphorylation and transcriptional activity of E2F1 during quiescent Pca cell re-entry. Quiescent LNCaP (A) and PC-3 (B) cells were initially stimulated to re-enter the cell cycle, and the effects of safranal (GI 90 ) on protein expression levels of p-AKT (Ser473), AKT, and E2F1 at specific intervals were determined by immunoblotting. GAPDH served as a loading control. The mRNA expression levels of E2F1 in LNCaP (C) and PC-3 (D) cells during cell cycle re-entry in the presence or absence of safranal (GI 90 ) were examined by RT-qPCR. E2F1 levels in cell nuclear and cytoplasm extracts of LNCaP (E) and PC-3 cells (F) were analyzed using immunoblotting after treatment with safranal for 12 and 3 h, respectively, following release from the quiescent state. α-Tubulin and lamin A/C served as loading and purity controls for the cytoplasm and nuclear fractions, respectively. Following a 6-day serum withdrawal for LNCaP and 2-day contact inhibition for PC-3 cells, quiescent LNCaP (G) and PC-3 (H) were transfected with pGM-E2F-Luc and renilla luciferase reporter plasmid pML-SV40-hRluc by using the EZ transfection agent for 15 h. The transfected quiescent cells were then induced to re-enter the cell cycle in the presence or absence of safranal (GI 90 ) for 24 h. Cell lysates were collected to assess firefly and renilla luciferase activities using the dual luciferase reporter assay kit. Renilla luciferase served to normalize the values of the experimental reporter gene and acted as an internal control for transfection efficiency. Data are expressed as mean ± SD. * P < 0.05, ** P < 0.01, *** P < 0.001 vs. dimethyl sulfoxide vehicle control.

Article Snippet: Then, the samples were stained with hematoxylin and eosin and with antibodies against Ki-67 (Abcam, ab16667), NF-κB p65 (Santa Cruz, sc514451), p-IκBα (Santa Cruz, sc8404), p21 (Proteintech, #10355-1-AP), CDK4 (Epitomics, #3830-1), CDK6 (Proteintech, #14052-1-AP), CDK2 (Abcam ab32147), p-Rb (Ser807, Abcam, ab184796), E2F1 (St John’s Laboratory, STJ92807), Skp2 (Santa Cruz, sc7164), c-MYC (Abcam, ab32072), and p27 (sc528, Santa Cruz).

Techniques: Phospho-proteomics, Activity Assay, Expressing, Western Blot, Control, Quantitative RT-PCR, Inhibition, Transfection, Luciferase, Plasmid Preparation, Reporter Assay

Journal: Frontiers in Cell and Developmental Biology

Article Title: Safrana l Prevents Prostate Cancer Recurrence by Blocking the Re-activation of Quiescent Cancer Cells via Downregulation of S-Phase Kinase-Associated Protein 2

doi: 10.3389/fcell.2020.598620

Figure Lengend Snippet: Safranal suppresses the tumor growth of quiescent PC-3 cells in vivo . Quiescent PC-3 cells were xenografted subcutaneously into the left flank of male nude mice. Then, the mice were orally administrated with either the vehicle control or 100 mg/kg of safranal, commencing a day prior to the xenograft procedure. Tumor volume (A) and mice body weight (B) were measured on alternate days. After a 46-day treatment, the mice were sacrificed, and the tumor (C) and major organs (D) were resected and photographed (scale bar: 20 μm). Paraffin-embedded tumor tissues were stained with hematoxylin and eosin and with antibodies against Ki-67, Skp2, E2F1, NF-κB p65, p-IκBα (Ser32), c-MYC, p-Rb (Ser807), CDK4, CDK6, CDK2, E2F1, Skp2, p21, and p27; representative images ( E , ×400) and quantification data (F) are presented. All data are shown as mean ± SD. * P < 0.05, ** P < 0.01, *** P < 0.001 vs. control.

Article Snippet: Then, the samples were stained with hematoxylin and eosin and with antibodies against Ki-67 (Abcam, ab16667), NF-κB p65 (Santa Cruz, sc514451), p-IκBα (Santa Cruz, sc8404), p21 (Proteintech, #10355-1-AP), CDK4 (Epitomics, #3830-1), CDK6 (Proteintech, #14052-1-AP), CDK2 (Abcam ab32147), p-Rb (Ser807, Abcam, ab184796), E2F1 (St John’s Laboratory, STJ92807), Skp2 (Santa Cruz, sc7164), c-MYC (Abcam, ab32072), and p27 (sc528, Santa Cruz).

Techniques: In Vivo, Control, Staining

Journal: Oncology reports

Article Title: miRNA-regulated expression of oncogenes and tumor suppressor genes in the cisplatin-inhibited growth of K562 cells.

doi: 10.3892/or_00000813

Figure Lengend Snippet: Figure 2. Changes in mRNA expression of BCL2, E2F1, E2F3, RB1 and P53 in K562 cells after cisplatin treatment. BCL2, E2F1, E2F3, RB1 and P53 were detected by (A) RT-PCR, (B) real-time PCR and (C) ELISA.

Article Snippet: K562 cells were lysed [lysis buffer: 0.15 M NaCl, 5 mM EDTA (pH 8.0), 1% Triton X-100, 10 mM Tris-Cl (pH 7.4), 100 mM PMSF and 5 M DTT) and incubated in a 96-well plate, followed by the addition of goat anti-human antibodies against BCL2 (1:400), E2F1, E2F3 (1:400, 1:400), RB1 (1:500) and P53 (1:300, 1:400) (all from Immunoleader, Boster) according to the manufacturer's instructions.

Techniques: Expressing, Reverse Transcription Polymerase Chain Reaction, Real-time Polymerase Chain Reaction, Enzyme-linked Immunosorbent Assay

Journal: Oncology reports

Article Title: miRNA-regulated expression of oncogenes and tumor suppressor genes in the cisplatin-inhibited growth of K562 cells.

doi: 10.3892/or_00000813

Figure Lengend Snippet: Figure 4. Correlative expression of miRNAs and oncogenes using antisense oligos (ASO). (A) RT-PCR, (B) real-time PCR. Correlative expression of (C) E2F1 and its targeted miR-17-5p, (D) E2F3 and its targeted miRNAs and (E) Bcl-2 and its targeted miRNAs (miR-16, 34a-c) using ELISA is shown. *Significant difference (p<0.05).

Article Snippet: K562 cells were lysed [lysis buffer: 0.15 M NaCl, 5 mM EDTA (pH 8.0), 1% Triton X-100, 10 mM Tris-Cl (pH 7.4), 100 mM PMSF and 5 M DTT) and incubated in a 96-well plate, followed by the addition of goat anti-human antibodies against BCL2 (1:400), E2F1, E2F3 (1:400, 1:400), RB1 (1:500) and P53 (1:300, 1:400) (all from Immunoleader, Boster) according to the manufacturer's instructions.

Techniques: Expressing, Reverse Transcription Polymerase Chain Reaction, Real-time Polymerase Chain Reaction, Enzyme-linked Immunosorbent Assay

Journal: Oncology reports

Article Title: miRNA-regulated expression of oncogenes and tumor suppressor genes in the cisplatin-inhibited growth of K562 cells.

doi: 10.3892/or_00000813

Figure Lengend Snippet: Figure 5. Expression of E2F1, E2F3, BCL2, RB1 and P53 genes regulated by miRNAs. Detection of the expression of (A) E2F1, (B) E2F3, (C) BCL2, (D) RB1 and (E) P53. *Significant difference (p<0.05).

Article Snippet: K562 cells were lysed [lysis buffer: 0.15 M NaCl, 5 mM EDTA (pH 8.0), 1% Triton X-100, 10 mM Tris-Cl (pH 7.4), 100 mM PMSF and 5 M DTT) and incubated in a 96-well plate, followed by the addition of goat anti-human antibodies against BCL2 (1:400), E2F1, E2F3 (1:400, 1:400), RB1 (1:500) and P53 (1:300, 1:400) (all from Immunoleader, Boster) according to the manufacturer's instructions.

Techniques: Expressing

Journal: Molecular Medicine Reports

Article Title: miR-329-3p regulates neural stem cell proliferation by targeting E2F1

doi: 10.3892/mmr.2019.10096

Figure Lengend Snippet: Sequences of mimics, siRNAs and primers used in the present study.

Article Snippet: The membranes were blocked by incubation in 2.5% (wt/vol) skimmed milk powder in TBS containing 0.1% Tween-20 (TBST) for 2 h at room temperature, washed with TBST 3 times, and then incubated at 4°C overnight with rabbit anti-rat polyclonal E2F1 antibody (1:500 dilution; cat. no. WL02394; Wanleibio Co., Ltd) or rabbit anti-rat polyclonal β-actin antibody (1:1,000 dilution; cat. no. WL0002c; Wanleibio Co., Ltd) diluted in TBST.

Techniques: Polymerase Chain Reaction

Journal: Molecular Medicine Reports

Article Title: miR-329-3p regulates neural stem cell proliferation by targeting E2F1

doi: 10.3892/mmr.2019.10096

Figure Lengend Snippet: Expression of miR-329-3p and E2F1 during NSC differentiation. (A) RT-qPCR analysis of miR-329-3p on days 0, 1, 3, and 5 after induction of neuronal differentiation. (B) RT-qPCR analysis of E2F1 mRNA expression and (C) western blot analysis of E2F1 protein expression on days 0, 1, 3, and 5 after induction of neuronal differentiation. GAPDH and β-actin were probed as internal controls for (B) and (C) respectively. (D) Quantification of protein expression. U6 was measured as an internal control for miR-329-3p. Data are presented as the mean ± standard deviation. *P<0.05 vs. day 0. miR, microRNA; E2F1, transcription factor E2F1; NSC, neural stem cell; RT-qPCR, reverse transcription quantitative polymerase chain reaction.

Article Snippet: The membranes were blocked by incubation in 2.5% (wt/vol) skimmed milk powder in TBS containing 0.1% Tween-20 (TBST) for 2 h at room temperature, washed with TBST 3 times, and then incubated at 4°C overnight with rabbit anti-rat polyclonal E2F1 antibody (1:500 dilution; cat. no. WL02394; Wanleibio Co., Ltd) or rabbit anti-rat polyclonal β-actin antibody (1:1,000 dilution; cat. no. WL0002c; Wanleibio Co., Ltd) diluted in TBST.

Techniques: Expressing, Quantitative RT-PCR, Western Blot, Control, Standard Deviation, Reverse Transcription, Real-time Polymerase Chain Reaction

Journal: Molecular Medicine Reports

Article Title: miR-329-3p regulates neural stem cell proliferation by targeting E2F1

doi: 10.3892/mmr.2019.10096

Figure Lengend Snippet: Direct targeting of E2F1 by miR-329-3p. (A) Putative miR-329-3p-binding sequence in the wt E2F1 3′-UTR. (B) Luciferase reporter assay of miR-329-3p targeting of E2F1 in 293 cells co-transfected with wt or mt E2F1 3′-UTR constructs and control or miR-329-3p mimics. (C) Reverse transcription quantitative polymerase chain reaction analysis and (D) western blot analysis of E2F1 mRNA and protein expression, respectively, in neural stem cells transfected with control or miR-329-3p mimics. GAPDH and β-actin were used as internal controls for (C) and (D) respectively. (E) Quantitative analysis of protein expression. Data are presented as the mean ± standard deviation. *P<0.05 vs. negative control mimic. E2F1, transcription factor E2F1; miR, microRNA; wt, wild-type; mt, mutated; UTR, untranslated region; rno, Rattus norvegicus .

Article Snippet: The membranes were blocked by incubation in 2.5% (wt/vol) skimmed milk powder in TBS containing 0.1% Tween-20 (TBST) for 2 h at room temperature, washed with TBST 3 times, and then incubated at 4°C overnight with rabbit anti-rat polyclonal E2F1 antibody (1:500 dilution; cat. no. WL02394; Wanleibio Co., Ltd) or rabbit anti-rat polyclonal β-actin antibody (1:1,000 dilution; cat. no. WL0002c; Wanleibio Co., Ltd) diluted in TBST.

Techniques: Binding Assay, Sequencing, Luciferase, Reporter Assay, Transfection, Construct, Control, Reverse Transcription, Real-time Polymerase Chain Reaction, Western Blot, Expressing, Standard Deviation, Negative Control

Journal: Molecular Medicine Reports

Article Title: miR-329-3p regulates neural stem cell proliferation by targeting E2F1

doi: 10.3892/mmr.2019.10096

Figure Lengend Snippet: Regulation of NSC proliferation by E2F1. NSCs were transfected with a control siRNA or E2F1-targeting siRNA for 48 h and then analyzed. (A) Reverse transcription quantitative polymerase chain reaction analysis of E2F1 mRNA expression and (B) western blot analysis of E2F1 protein expression. GAPDH and β-actin were probed as internal controls for (A) and (B) respectively. (C) Quantification of protein expression. (D) Cell Counting Kit-8 assay of NSC proliferation. (E) EdU assay of NSC proliferation. Red and blue staining indicates EdU and Hoechst nuclear staining, respectively. Scale bar, 50 µm. (F) Quantification of the EdU-positive cells. Data are presented as the mean ± standard deviation. *P<0.05 vs. siRNA control. NSCs, neural stem cells; E2F1, transcription factor E2F1; siRNA, small interfering RNA; NC, negative control; OD, optical density; EdU, 5-ethynyl-2′-deoxyuridine.

Article Snippet: The membranes were blocked by incubation in 2.5% (wt/vol) skimmed milk powder in TBS containing 0.1% Tween-20 (TBST) for 2 h at room temperature, washed with TBST 3 times, and then incubated at 4°C overnight with rabbit anti-rat polyclonal E2F1 antibody (1:500 dilution; cat. no. WL02394; Wanleibio Co., Ltd) or rabbit anti-rat polyclonal β-actin antibody (1:1,000 dilution; cat. no. WL0002c; Wanleibio Co., Ltd) diluted in TBST.

Techniques: Transfection, Control, Reverse Transcription, Real-time Polymerase Chain Reaction, Expressing, Western Blot, Cell Counting, EdU Assay, Staining, Standard Deviation, Small Interfering RNA, Negative Control

Journal: Molecular Medicine Reports

Article Title: miR-329-3p regulates neural stem cell proliferation by targeting E2F1

doi: 10.3892/mmr.2019.10096

Figure Lengend Snippet: miR-329-3p-mediated regulation of NSC proliferation via E2F1. (A) Reverse transcription quantitative polymerase chain reaction analysis of E2F1 mRNA expression and (B) western blot analysis of E2F1 protein expression. GAPDH and β-actin were probed as internal controls for (A) and (B) respectively. (C) Quantitative analysis of protein expression. (D) Cell Counting Kit-8 assay of NSC proliferation. (E) EdU assay of NSC proliferation. Red and blue indicate EdU and Hoechst nuclear staining, respectively. Scale bar, 50 µm. (F) Quantification of EdU-positive cells. Data are presented as the mean ± standard deviation. *P<0.05 vs. control. miR, microRNA; NSC, neural stem cell; E2F1, transcription factor E2F1; OD, optical density; EdU, 5-ethynyl-2′-deoxyuridine.

Article Snippet: The membranes were blocked by incubation in 2.5% (wt/vol) skimmed milk powder in TBS containing 0.1% Tween-20 (TBST) for 2 h at room temperature, washed with TBST 3 times, and then incubated at 4°C overnight with rabbit anti-rat polyclonal E2F1 antibody (1:500 dilution; cat. no. WL02394; Wanleibio Co., Ltd) or rabbit anti-rat polyclonal β-actin antibody (1:1,000 dilution; cat. no. WL0002c; Wanleibio Co., Ltd) diluted in TBST.

Techniques: Reverse Transcription, Real-time Polymerase Chain Reaction, Expressing, Western Blot, Cell Counting, EdU Assay, Staining, Standard Deviation, Control

Journal: PLoS ONE

Article Title: Cell Killing Mechanisms and Impact on Gene Expression by Gemcitabine and 212 Pb-Trastuzumab Treatment in a Disseminated i.p. Tumor Model

doi: 10.1371/journal.pone.0159904

Figure Lengend Snippet: Mice bearing i.p. LS-174T xenografts were pre-treated with Gem followed 24 h thereafter with 212 Pb-RIT. (A) Expression of BRCA1 , CHK1 , MSH2 and was determined by qRT-PCR. Results represent the average of a minimum of three replications. (B) Binding abundance to E2F1 was determined by ChIP using specific primers for CHK1 and MSH2 . (C) Immunoblot analysis for RAD51B and XRCC2 was performed with tumor tissue collected as described. RAD51B and XRCC2 were detected at 32 kDa and 42 kDa, respectively. Equal protein loading control was GAPDH.

Article Snippet: Chromatin was immunoprecipitated with 10 μL (1:100) of antibody for E2F1 (Upstate Biotechnology).

Techniques: Expressing, Quantitative RT-PCR, Binding Assay, Western Blot, Control

Journal: PLoS ONE

Article Title: Cell Killing Mechanisms and Impact on Gene Expression by Gemcitabine and 212 Pb-Trastuzumab Treatment in a Disseminated i.p. Tumor Model

doi: 10.1371/journal.pone.0159904

Figure Lengend Snippet: Mice bearing i.p. LS-174T xenografts were pre-treated with Gem followed 24 h thereafter with 212 Pb-RIT. (A) Expression of p73 , NOXA , p53AIP1 , and PUMA was determined by qRT-PCR. Results represent the average of a minimum of three replications. (B) Binding abundance to E2F1 was determined by ChIP using specific primer for p73 . (C) Immunohistochemical analysis using γH 2 AX and H&E staining was performed with tumor tissue collected as described.

Article Snippet: Chromatin was immunoprecipitated with 10 μL (1:100) of antibody for E2F1 (Upstate Biotechnology).

Techniques: Expressing, Quantitative RT-PCR, Binding Assay, Immunohistochemical staining, Staining

Journal: BMC Research Notes

Article Title: Expressions and clinical significances of c-MET, p-MET and E2f-1 in human gastric carcinoma

doi: 10.1186/1756-0500-7-6

Figure Lengend Snippet: Immunohistochemical staining for the primary foci of GC (SABC method). Notes: A : c-MET; B : p-MET, C : Ki-67; D : e2f-1.

Article Snippet: The rabbit-derived monoclonal antibodies against human such as anti-c-MET (D1C2; Cell Signaling TECHNOLOGY, Beverly, MA), anti-c-MET phosphorylation (p-MET) (Tyr1234/1235; D26; Cell Signaling TECHNOLOGY, Beverly, MA), anti-e2f-1 (USCN Life Science Ltd Co., Wuhan, China) and anti-Ki-67 (USCN Life Science Ltd Co., Wuhan, China) were used.

Techniques: Immunohistochemical staining, Staining

Journal: BMC Research Notes

Article Title: Expressions and clinical significances of c-MET, p-MET and E2f-1 in human gastric carcinoma

doi: 10.1186/1756-0500-7-6

Figure Lengend Snippet: Expression of e2f-1 and its correlation with clinicopathological parameters in 121 cases

Article Snippet: The rabbit-derived monoclonal antibodies against human such as anti-c-MET (D1C2; Cell Signaling TECHNOLOGY, Beverly, MA), anti-c-MET phosphorylation (p-MET) (Tyr1234/1235; D26; Cell Signaling TECHNOLOGY, Beverly, MA), anti-e2f-1 (USCN Life Science Ltd Co., Wuhan, China) and anti-Ki-67 (USCN Life Science Ltd Co., Wuhan, China) were used.

Techniques: Expressing

Journal: BMC Research Notes

Article Title: Expressions and clinical significances of c-MET, p-MET and E2f-1 in human gastric carcinoma

doi: 10.1186/1756-0500-7-6

Figure Lengend Snippet: Chi-square analysis and Spearson relative analysis among the expression of c-MET, p-MET, e2f-1 and Ki-67

Article Snippet: The rabbit-derived monoclonal antibodies against human such as anti-c-MET (D1C2; Cell Signaling TECHNOLOGY, Beverly, MA), anti-c-MET phosphorylation (p-MET) (Tyr1234/1235; D26; Cell Signaling TECHNOLOGY, Beverly, MA), anti-e2f-1 (USCN Life Science Ltd Co., Wuhan, China) and anti-Ki-67 (USCN Life Science Ltd Co., Wuhan, China) were used.

Techniques: Expressing

Journal: BMC Research Notes

Article Title: Expressions and clinical significances of c-MET, p-MET and E2f-1 in human gastric carcinoma

doi: 10.1186/1756-0500-7-6

Figure Lengend Snippet: Survival analysis on all case by Log-rank analysis (n = 121)

Article Snippet: The rabbit-derived monoclonal antibodies against human such as anti-c-MET (D1C2; Cell Signaling TECHNOLOGY, Beverly, MA), anti-c-MET phosphorylation (p-MET) (Tyr1234/1235; D26; Cell Signaling TECHNOLOGY, Beverly, MA), anti-e2f-1 (USCN Life Science Ltd Co., Wuhan, China) and anti-Ki-67 (USCN Life Science Ltd Co., Wuhan, China) were used.

Techniques: Expressing

Journal: BMC Research Notes

Article Title: Expressions and clinical significances of c-MET, p-MET and E2f-1 in human gastric carcinoma

doi: 10.1186/1756-0500-7-6

Figure Lengend Snippet: Postoperative long-term survival curves regarding to the expression of c-MET, p-MET and e2f-1. Notes: A : Grouped by c-MET expression (P = 0.001); B ; Grouped by p-MET expression (P = 0.001). C : Grouped by e2f-1 expression (P = 0.036). D : In patients with stages I and II grouped by e2f-1 positive and negative expression; E : In patients with stages III and IV grouped by e2f-1 positive and negative expression.

Article Snippet: The rabbit-derived monoclonal antibodies against human such as anti-c-MET (D1C2; Cell Signaling TECHNOLOGY, Beverly, MA), anti-c-MET phosphorylation (p-MET) (Tyr1234/1235; D26; Cell Signaling TECHNOLOGY, Beverly, MA), anti-e2f-1 (USCN Life Science Ltd Co., Wuhan, China) and anti-Ki-67 (USCN Life Science Ltd Co., Wuhan, China) were used.

Techniques: Expressing

Journal: BMC Research Notes

Article Title: Expressions and clinical significances of c-MET, p-MET and E2f-1 in human gastric carcinoma

doi: 10.1186/1756-0500-7-6

Figure Lengend Snippet: Stratified survival analysis of e2f-1 expression in groups with different TNM stage

Article Snippet: The rabbit-derived monoclonal antibodies against human such as anti-c-MET (D1C2; Cell Signaling TECHNOLOGY, Beverly, MA), anti-c-MET phosphorylation (p-MET) (Tyr1234/1235; D26; Cell Signaling TECHNOLOGY, Beverly, MA), anti-e2f-1 (USCN Life Science Ltd Co., Wuhan, China) and anti-Ki-67 (USCN Life Science Ltd Co., Wuhan, China) were used.

Techniques: Expressing