Journal: Science Progress

Article Title: Low-dose urapidil mitigates renal ischemia-reperfusion injury through matrix metalloproteinase-9 inhibition and anti-inflammatory effects

doi: 10.1177/00368504261438886

Figure Lengend Snippet: Comparison of MMP-9 IHC between the kidney samples from each group. Details of MMP-9 marking were observed with IHC. MMP-9, matrix metalloproteinase-9; IHC, immunohistochemistry; SH, sham group; CR, control group; I/R, ischemia-reperfusion injury; UP, urapidil.

Article Snippet: Following the completion of the blocking procedure, the samples were treated with primary antibodies, including eNOS (Boster Bio., catalog number: A01604-2, diluted at a ratio of 1:250), caspase 3 (Thermo Scıentıfıc, catalog number: RB-1197-P0, diluted at a ratio of 1:100), TNF-α (Proteintech, catalog number:60291-1-Ig, diluted at a ratio of 1:300), IL-1β (Bioss, catalog number:bs-6319R, diluted at a ratio of 1:150), IL-6 (ST John’s, catalog number:STJ1110424, diluted at a ratio of 1:100), and MMP-9 (Boster Bio, catalog number: PA2140-2, diluted at a ratio of 1:100).

Techniques: Comparison, Immunohistochemistry, Control

Journal: Heliyon

Article Title: Modified Zhenwu Tang delays chronic renal failure progression by modulating oxidative stress and hypoxic responses in renal proximal tubular epithelial cells.

doi: 10.1016/j.heliyon.2024.e31265

Figure Lengend Snippet: Fig. 3. Adenine-induced oxidative stress, activation of the HIF pathway, and increased production of inflammatory factors and tubulointerstitial collagen fibers in rat proximal renal tubules, with MZWT mitigating these effects. (a) Immunohistochemical detection of SOD1, MDA, HIF-1α, COL1A1, IL-1β, TNF-α in rat renal tissues (IHC × 400). (b) Quantitative analysis of the positive expression areas for each marker. (N: normal group, M: model group, MZWT: Modified Zhenwu Tang group, Lotensin: benazepril hydrochloride group. ##P < 0.01, #P < 0.05 compared with the N group; **P < 0.01, *P < 0.05 compared with the M group; NS indicates no statistical significance).

Article Snippet: Zhang et al. Heliyon 10 (2024) e31265 temperature, and incubated with α-SMA and COL1A1 antibodies (1:100, boster, BM0002, bs-10423).

Techniques: Activation Assay, Immunohistochemical staining, Immunohistochemistry, Expressing, Marker, Modification

Journal: Heliyon

Article Title: Modified Zhenwu Tang delays chronic renal failure progression by modulating oxidative stress and hypoxic responses in renal proximal tubular epithelial cells.

doi: 10.1016/j.heliyon.2024.e31265

Figure Lengend Snippet: Fig. 12. Expression of fibrotic markers α-SMA and COL1A1 in HK-2 cells under hypoxic conditions and LPS treatment, showing reduction by MZWT (IF × 200). Details of expression: (a) α-SMA, (b) Relative intensity of α-SMA, (c) COL1A1, (d) Relative intensity of COL1A1. Groups: (A: Normal group, B: Model group, C: Blank serum group, D: Drug serum group, E: FM19G11 group, F: NAC group). Statistical significance: ##P < 0.01, #P < 0.05 compared with the A group; **P < 0.01, *P < 0.05 compared with the B group; NS indicates no statistical significance.

Article Snippet: Zhang et al. Heliyon 10 (2024) e31265 temperature, and incubated with α-SMA and COL1A1 antibodies (1:100, boster, BM0002, bs-10423).

Techniques: Expressing

Journal: Experimental Hematology & Oncology

Article Title: Dickkopf-1 promotes tumor progression of gefitinib- resistant non-small cell lung cancer through cancer cell-fibroblast interactions

doi: 10.1186/s40164-025-00616-9

Figure Lengend Snippet: Decreases in myofibroblast characteristics of MRC-5 by DKK1. ( A ) Volcano plot for secretory genes using RNA-sequencing data from Fig. A. Red dots represent genes satisfying p-value < 0.05 (left). GO term analysis for secreted protein genes satisfying p-value < 0.05 (right). ( B ) Myofibroblast marker genes in MRC-5 treated with HCC827-DKK1-OE culture supernatants. The data were obtained using RNA-sequencing data from Fig. A. ( C ) Col1a1 , ACTA2 mRNA expression in MRC-5 treated with HCC827-DKK1-OE culture supernatants for 6 h. ( D ) Col1a1 and α-SMA protein expression in MRC-5 treated with HCC827-DKK1-OE culture supernatants for 48 h. ( E ) TGF-β concentration in culture media and supernatant obtained from HCC827-LV con and HCC827-DKK1 OE. ( F ) Masson’s trichrome staining using lung tissue obtained from Fig. F. Representative images (left) and collagen area fraction (%) analyzed by ImageJ software (right). ( G ) Protein levels of Col1a1 and a-SMA in tumor sample obtained from Fig. I. All statistical significance of the differences was determined by unpaired two-tailed Student t-test. ns, non-significant; *, P < 0.05; **, P < 0.01; ***, P < 0.001; ****, P < 0.0001

Article Snippet: Antibodies targeting DKK1 (10170-R015, SinoBiological, Beijing, China), glyceraldehyde 3-phosphate dehydrogenase (GAPDH, CB1001, Merck Millipore, Burlington, MA, USA), JNK (3496-1, Epitomics, Burlingame, CA, USA), Col1a1 (PB9938, Boster Biological Technology, Pleasanton, CA, USA), α-smooth muscle actin (α-SMA, A5228, Sigma-Aldrich, St. Louis, MO, USA) were used.

Techniques: RNA Sequencing, Marker, Expressing, Concentration Assay, Staining, Software, Two Tailed Test