Journal: PLoS ONE

Article Title: T Cell Leukemia/Lymphoma 1A is essential for mouse epidermal keratinocytes proliferation promoted by insulin-like growth factor 1

doi: 10.1371/journal.pone.0204775

Figure Lengend Snippet: (A) Immunofluorescence of cFOS (green), or (B) cJUN (red) and phospho-cJUN (green) on WT, Tcl1-/- and K14-TCL1 mice epidermis. Nuclear counterstain is propidium iodide (blue).Merging of green and blue fluorescence represents nuclear localization (light blue). Magnification: 40X. Images were acquired by confocal laser scanning. (C) Western blot analysis of cJUN and phospho-cJUN on protein extracts of KCs from WT, Tcl1-/- and K14-TCL1 mice. (D) Normalized OD values were used to calculate the ratio of phosphorylated to entire form and graphed as the mean and standard deviation of three independent experiments for the WT (dark gray), Tcl1-/- (light gray) and K14-TCL1 (black) mice.

Article Snippet: 5μm sections were cut, processed as described [ ] and incubated overnight at 4°C with anti-TCL1 (5A4 rat monoclonal antibody from Areta International), anti-phospho-AKT (9271 Cell Signaling Technology), anti-cFOS (PC38 Calbiochem-MERK), anti-cJUN (610326 BD biosciences), anti-phospho-cJUN (3270 Cell Signaling Technology), anti-KRT6A (PRB-169P) or anti-KRT10 (PRB-159P Covance-Biolegend).

Techniques: Immunofluorescence, Fluorescence, Western Blot, Standard Deviation