Journal: iScience

Article Title: Identification of CX3CR1 + mononuclear phagocyte subsets involved in HIV-1 and SIV colorectal transmission

doi: 10.1016/j.isci.2022.104346

Figure Lengend Snippet:

Article Snippet: CD103 , AbDSerotec , Cat# MCA708T; RRID: AB_1100549.

Techniques: Quantitative RT-PCR, Enzyme-linked Immunosorbent Assay, Software

Journal: Cell reports. Medicine

Article Title: Tissue-resident memory CAR T cells with stem-like characteristics display enhanced efficacy against solid and liquid tumors.

doi: 10.1016/j.xcrm.2023.101053

Figure Lengend Snippet: Figure 2. CAR-TRM cells possess a core resident memory transcriptomic signature Bulk RNA-seq experiments were conducted using CD8+ CAR-TCONV and CAR-TRM cells. (A) Principal-component analysis was carried out with RNA expression data. (B) Gene set enrichment analysis (GSEA) using gene sets associated with resident memory T cells. (C) Heatmap showing differentially expressed genes from CD69+CD103+ and CD69CD103CD8+ T cell signatures. (D) Heatmap displaying expression of transcription factor genes known to promote and suppress resident memory T cell differentiation. (E) GSEA (hypoxia pathways: MSigDB C2). (F) Pathway analysis incorporating genes upregulated in CAR-TRM-cells using Enrichr. (G) GSEA analysis (migration pathways: MSigDB C2). GSEA with gene signatures of tumor-infiltrating T cells associated with response to ICB. Bulk RNA-seq experiments were conducted using CAR T cells derived from n = 3 distinct healthy donors, serving as biological replicates.

Article Snippet: To examine the role of the resident memory population in mediating CAR-TRM cell antitumor responses, the CD103 + population in CAR-TRM products was magnetically depleted using biotinylated anti-human CD103 antibody and anti-biotin microbeads (Miltenyi Biotec).

Techniques: RNA Sequencing, RNA Expression, Expressing, Cell Differentiation, Migration, Derivative Assay

Journal: Cell reports. Medicine

Article Title: Tissue-resident memory CAR T cells with stem-like characteristics display enhanced efficacy against solid and liquid tumors.

doi: 10.1016/j.xcrm.2023.101053

Figure Lengend Snippet: Figure 3. TGF-b-generated CAR-TRM cells transcriptionally resemble stem-like TRM cells (A) UMAP imbedding of CD8+CAR T cell (top, CD8 subclusters; middle, CAR T cell samples; bottom, cell cycle). Frequencies of three CD8+ clusters in CAR-TCONV and CAR-TRM cells are shown. (B) Bubble plot displaying expression levels of marker genes in each CD8+ subcluster. (C) Violin plots showing expression of KLF2 and ITGAE.

Article Snippet: To examine the role of the resident memory population in mediating CAR-TRM cell antitumor responses, the CD103 + population in CAR-TRM products was magnetically depleted using biotinylated anti-human CD103 antibody and anti-biotin microbeads (Miltenyi Biotec).

Techniques: Generated, Expressing, Marker

Journal: Cell reports. Medicine

Article Title: Tissue-resident memory CAR T cells with stem-like characteristics display enhanced efficacy against solid and liquid tumors.

doi: 10.1016/j.xcrm.2023.101053

Figure Lengend Snippet: Figure 7. Comparison of CAR-TRM cell differentiation methods (A) RUNX3 expression measured in various anti-mesothelin SS1 CAR T cell products: standard T cell medium culture (control CAR T), standard T cell medium supplemented with TGF-b (TGF-b CAR T) and engineered to overexpress RUNX3 (RUNX3 OE CAR T) (MFI, geometric mean fluorescence intensity). (B) TRM (CD103, CD39, CD49a) and activation (CD25) markers were assessed after CAR T cell manufacturing. (C) NSG mice (n = 7 biological replicates per group) were injected with the EMMESO cell line. Once tumors reached a size of 120 mm3, mice received a single dose of 1 3 107 SS1 CAR T cells. Activated non-transduced T cells served as a negative control. *p < 0.05; **p < 0.01; ***p < 0.001; ns, not significant (one-way ANOVA).

Article Snippet: To examine the role of the resident memory population in mediating CAR-TRM cell antitumor responses, the CD103 + population in CAR-TRM products was magnetically depleted using biotinylated anti-human CD103 antibody and anti-biotin microbeads (Miltenyi Biotec).

Techniques: Comparison, Cell Differentiation, Expressing, Control, Activation Assay, Injection, Negative Control

Journal: Journal for Immunotherapy of Cancer

Article Title: Development of 89 Zr-anti-CD103 PET imaging for non-invasive assessment of cancer reactive T cell infiltration

doi: 10.1136/jitc-2022-004877

Figure Lengend Snippet: In vivo anti-mCD103 mAb microPET imaging. (A) Representative coronal 89Zr-anti-M290 and 89Zr-isotype control PET scans, 1, 3 and 6 days post tracer injection in non-tumor bearing C57BL/6 mice. (B) Ex vivo tissue biodistribution of 89Zr-anti-M290 and 89Zr-isotype control in non-tumor bearing C57BL/6 mice, 6 days post-tracer injection (median, n=3). (C) Analysis of ex vivo macroscopic tracer distribution in FFPE tumor tissue slices using autoradiography. (D) immunohistochemical CD103 staining of the small intestine, mesenteric lymph node (left) and colon (right). ALN, axillary lymph node; BLN, brachial lymph node; MLN, mesenteric lymph node.

Article Snippet: Anti-mCD103 M290 (BioXCell; #BE0026), IgG2a isotype control (BioXCell; #BE0089), hCD103.01A and hCD103.05A were incubated with a sevenfold, fivefold, fourfold, and fourfold molar excess of TFP-N-Suc-desferal-Fe (Df, ABX, Hamburg, Germany), respectively.

Techniques: In Vivo, Imaging, Control, Injection, Ex Vivo, Autoradiography, Immunohistochemical staining, Staining

Journal: Cell reports

Article Title: High-dimensional profiling clusters asthma severity by lymphoid and non-lymphoid status

doi: 10.1016/j.celrep.2021.108974

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Anti-Human CD103 (Ber-ACT8)-151Eu , Fluidigm , Cat# 3151011B; RRID:AB_2756418.

Techniques: Purification, Antibody Labeling, Flow Cytometry, Software, Staining, Blocking Assay