Journal: Parasites & Vectors

Article Title: Starvation of low-density lipoprotein-derived cholesterol induces bradyzoite conversion in Toxoplasma gondii

doi: 10.1186/1756-3305-7-248

Figure Lengend Snippet: IFAT analysis of BAG1 expression in parasite vacuoles. CHO cells were infected with T. gondii ME49. After 72 h, the parasite-infected cells were subjected to IFAT analyses. (A) Infected CHO cells were stained with a SAG1 antibody (tachyzoite specific marker) and a BAG1 antibody (bradyzoite specific marker). Scale bar: 10 μm. (B) Percentage of vacuoles expressing BAG1. Data represent the mean percentage of BAG-positive vacuoles from one hundred vacuoles (from triplicate samples). Statistical analysis of the data was conducted using a one-way ANOVA followed by Tukey’s multiple comparison tests. Different symbols indicate statistically significant differences ( P < 0.01). SS, 50 μM squalestatin.

Article Snippet: The coverslips were incubated with BAG1 rabbit anti-serum and an anti-SAG1 mouse monoclonal antibody (clone TP3; Advanced ImmunoChemical Inc., Long Beach, CA) diluted at 1:100 in 3% BSA in PBS++ for 1 h at room temperature.

Techniques: Expressing, Infection, Staining, Marker, Comparison

Journal: Journal of Biological Chemistry

Article Title: Hip Is a Pro-survival Substrate of Granzyme B

doi: 10.1074/jbc.m704312200

Figure Lengend Snippet: FIGURE 2. Proteolysis of chaperone family proteins in cell lysates. Granzyme B was added to cell lysates as described under “Experimental Procedures.” Immunoblots are to Hip (A); Hsp90 and (left panel), Hsp90 (middle panel), and Hsp90 (right panel) (B); and Bag1 (C). The blots in B were overexposed to facilitate visual- ization of the proteolytic products. A schematic of Bag1-L showing the predicted granzyme B cleavage sites is below the Bag1 immunoblot.

Article Snippet: The anti-Bag1 antibody was from Stressgen (catalog number AAM-400).

Techniques: Western Blot