Journal: Journal of Medicinal Chemistry

Article Title: Putative Kappa Opioid Heteromers As Targets for Developing Analgesics Free of Adverse Effects

doi: 10.1021/jm500159d

Figure Lengend Snippet: Representative high power fluorescent micrographs of control HEK293 cells and those singly and doubly expressing opioid receptors that were stained for β-arrestin2. INTA ( 3 )- and NNTA-treated MOR-KOR HEK293 cells showed some recruitment of β-arrestin2, while INTA-treated KOR-DOR HEK293 cells showed no recruitment of β-arrestin2. 6′-GNTI-treated KOR-DOR HEK 293 cells exhibited little recruitment. Treatment of nontransfected HEK293 cells with 3 , NNTA ( 1 ), 6′-GNTI ( 2 ), or any of the standard opioid control ligands did not induce β-arrestin2 recruitment (not shown).

Article Snippet: Anti-β-arrestin2 (goat) antibodies (R&D Systems) were added to each well at a concentration of 1 μg/mL and incubated at 4 ° C overnight on an oscillating shaker plate.

Techniques: Control, Expressing, Staining

Journal: Cellular and Molecular Life Sciences

Article Title: A look into retinal organoids: methods, analytical techniques, and applications

doi: 10.1007/s00018-021-03917-4

Figure Lengend Snippet: A wide range of antibodies is available for IHC of retinal organoids

Article Snippet: ARR3 , Cone photoreceptor, stage 3 , Goat , Novus Biologicals , NBP137003 , 1/200 , [ , ] .

Techniques: Marker, Clinical Proteomics, Membrane