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Bioss
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Rockland Immunochemicals
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Proteintech
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Cell Signaling Technology Inc
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Cell Signaling Technology Inc
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Cell Signaling Technology Inc
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Cell Signaling Technology Inc
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Cell Signaling Technology Inc
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Cell Signaling Technology Inc
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Cell Signaling Technology Inc
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Santa Cruz Biotechnology
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Cell Signaling Technology Inc
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Image Search Results
Journal: BMJ Open Diabetes Research & Care
Article Title: Mouse model of metformin-induced diarrhea
doi: 10.1136/bmjdrc-2019-000898
Figure Lengend Snippet: Effects of wood creosote on diabetic obese mice treated with metformin. Wood creosote does not affect efficacy of metformin. Weight change (A) and blood glucose levels (fed condition) (B) were examined during the experiment in . Data are shown as means±SEM. No significant differences between the metformin group and the metformin with wood creosote (M+C) group were observed. Mice were fasted for 3 hours after the final administration of drugs. (C) One g/kg glucose was administrated via oral gavage. One to 2 µL blood was collected via tail vein at indicated time points, and blood glucose was measured by using GlucoSensor. (D) At day 4 of , mice were fasted for 2 hours and then injected intraperitoneally with 36 µg/kg insulin. Blood glucose levels are shown as % initial. At day 5 in , blood was collected and measured serum bile acid levels (E) and alanine aminotransferase (F) were measured. (G) Western blots were carried out using liver protein (50 µg) to detect levels of phospho-AMPK and phoshopho-AKT. AKT, Protein Kinase B; AMPK, AMP-activated kinase.
Article Snippet: Antibodies are as follows: Anti-glyceraldehyde 3-phosphate dehydrogenase (GAPDH) (1E6D9, Proteintech, Rosemont, Illinois, USA), anti-AMPK (ABV10739, ABGENT, San Diego, California, USA), anti-phospho AMPK (pT172) (40H9, Cell Signaling Technology, Danvers, Massachusetts, USA),
Techniques: Injection, Western Blot