Journal: International journal of oncology

Article Title: β-elemene inhibits stemness, promotes differentiation and impairs chemoresistance to temozolomide in glioblastoma stem-like cells.

doi: 10.3892/ijo.2014.2448

Figure Lengend Snippet: Figure 5. The expression of CD133 and ABCG2 is decreased, and the expression of GFAP is increased by β-elemene in GSC spheres. (A and B) β-elemene downregulated the expression of CD133 and ABCG2 and upregulated the expression of GFAP dose-dependently in case 1 and case 2. (C and D) The results of (A) and (B) were semi-quantitatively estimated using Gel-Pro Analyzer 4.0 software and illustrated in a polygram. The results are representative of three independent experiments, and values shown are the means ± SD (*P<0.05, **P<0.01).

Article Snippet: The antibodies against CD133, ABCG2, GFAP, neuron specific enolase (NSE), and myelin basic protein (MBP), used in western blots and immunofluorescence assays, were purchased from Boster Co., Ltd. (Wuhan, China).

Techniques: Expressing, Software

Journal: International journal of oncology

Article Title: β-elemene inhibits stemness, promotes differentiation and impairs chemoresistance to temozolomide in glioblastoma stem-like cells.

doi: 10.3892/ijo.2014.2448

Figure Lengend Snippet: Figure 7. β-elemene decreases the expression of CD133 and ABCG2 and increases the expression of GFAP in vivo. (A) After the tumour tissues from GSC- transplanted nude mice treated with β-elemene or NaCl in the previous experiment were prepared following routine protocols, sample slides were probed with antibodies against CD133, ABCG2 and GFAP. (B and C) The IOD of each group was analysed using Image-Pro Plus 6.0 software (the same selected areas and magnifications in all the images) and illustrated by a vertical bar chart. The IODs for CD133 and ABCG2 were lower and the IOD for GFAP significantly higher in the β-elemene group than in the NaCl group. Serial sections stained with control antibodies were negative (data not shown). Values shown are the means ± SD (*P<0.05, **P<0.01). Original magnifications x200.

Article Snippet: The antibodies against CD133, ABCG2, GFAP, neuron specific enolase (NSE), and myelin basic protein (MBP), used in western blots and immunofluorescence assays, were purchased from Boster Co., Ltd. (Wuhan, China).

Techniques: Expressing, In Vivo, Software, Staining, Control

Journal: British Journal of Cancer

Article Title: Impact of mutant β -catenin on ABCB1 expression and therapy response in colon cancer cells

doi: 10.1038/bjc.2012.81

Figure Lengend Snippet: Expression of ABCC1, ABCG2 and MVP is independent of gain-of-function β -catenin. ABCC1, ABCG2 and MVP were not differentially expressed in cells with different β -catenin genotype. ( A ) ABCC1, ABCG2 and MVP mRNA expressions were determined by quantitative real-time RT–PCR; values represent the ratios of gene and G6PDH control mRNA (HCT116 cells: set 100%). Experiments were performed in duplicate and average values are shown. At the protein level, no significantly different expression levels of ABCC1, ABCG2 and MVP have been detected by western blot analysis ( B ) or by immuno flow cytometry ( C , D ).

Article Snippet: Membranes were incubated overnight at 4 °C with a monoclonal anti-ABCB1 antibody (C219, Novus Biologicals, Cambridge, UK; 1 : 50), a monoclonal anti-ABCC1 antibody (MRPm6, Acris antibodies; 1 : 50), a monoclonal anti-ABCG2 antibody (BXP-21, Abnova; 1 : 50) and a monoclonal anti-MVP antibody (MVP 1014, Gene Tex; 1 : 50).

Techniques: Expressing, Quantitative RT-PCR, Control, Western Blot, Flow Cytometry