Journal: Frontiers in Bioengineering and Biotechnology

Article Title: A novel method for the establishment of autologous skin cell suspensions: characterisation of cellular sub-populations, epidermal stem cell content and wound response-enhancing biological properties

doi: 10.3389/fbioe.2024.1386896

Figure Lengend Snippet: Proteomic analysis of the ACS secretome. Semiquantitative analysis using proteome arrays and quantitative detection by ELISA assays of soluble mediators secreted by ACS-derived cell populations cultured in serum-free medium at the indicated time points. Supernatants from cultures established from ACS were collected and analysed as detailed in the Methods. (A) Relative intensity values of densitometric analysis of ACS-secreted soluble factors using an angiogenesis array on days 1, 3 and 6 after isolation. (B) Relative intensity values of similar analysis using a cytokine proteome array on the same time-points post-isolation. (C) Detection of Interleukin-1 alpha, HMGB1 and Hsp90α in the ACS secretome by analyte-specific ELISA. *, p < 0.05; **, p < 0.01; ***, p < 0.001; ****, p < 0.0001; ns, non-significance.

Article Snippet: Detection of analytes released in vitro from collected conditioned medium (at the indicated time points following culture initiation) from ACS-derived cultures was performed using an angiogenesis array kit (#ARY007) and a cytokine array kit (#ARY005B) (both from R&D Systems, supplied by Bio-Techne, Abingdon, United Kingdom).

Techniques: Enzyme-linked Immunosorbent Assay, Derivative Assay, Cell Culture, Isolation

Journal: Journal of Cerebral Blood Flow & Metabolism

Article Title: Non-classical monocytes promote neurovascular repair in cerebral small vessel disease associated with microinfarctions via CX3CR1

doi: 10.1177/0271678X231183742

Figure Lengend Snippet: Non-classical monocytes promote vascular function in cSVD. (a) Representative LSCI showing the temporal changes in the CBF of WT C and MDP mice up to 7 days post-cSVD. Analysis of CBF (b) at baseline (c) 24 h, (d) 3 days and (e) 7 days post-cSVD, shown as ipsilateral/contralateral (I/C) ratio. (f) Representative images of the Proteome Profiler mouse angiogenesis array membranes profiling expression of proteins implicated in vascular remodeling in the serum of CX3CR1 GFP/+ C and MDP mice 3 days post-cSVD. Analysis of the expression (optical density) of (g) CD105, (h) NOV/CCN3, (i) PDGF-AA and (j) VEGF, (k) serpin E1, (l) serpin F1, (m) thrombospondin 2 and (n) TIMP-4 in the serum of CX3CR1 GFP/+ C and MDP mice. Data are boxplot with min/max (n = 4–5 animals/group; n = 4 dots/experimental condition). I/C ratio = 1 indicates similar pattern in ipsilateral and contralateral hemispheres. *P < 0.05/**P < 0.01/***P < 0.001/ **** P < 0.0001 compared to WT C mice or CX3CR1 GFP/+ C mice (unpaired two-tailed t -test). Statistical summary is provided in (Supplementary Material 2). D, days.

Article Snippet: Blood was collected and allowed to clot in Eppendorf tubes for 2 h at RT and centrifuged at 2000×g for 20 min. Supernatant was collected and processed in a membrane-based Proteome Profiler TM mouse angiogenesis array kit (R&D, ARY015), following manufacturer’s recommendations.

Techniques: Expressing, Two Tailed Test

Journal: eLife

Article Title: The adhesion-GPCR BAI1 shapes dendritic arbors via Bcr-mediated RhoA activation causing late growth arrest

doi: 10.7554/eLife.47566

Figure Lengend Snippet:

Article Snippet: Antibody , anti-BAI2 (extracellular) (rabbit polyclonal) , Alomone Labs , Alomone:ABR-022; RRID: AB_2756544 , (1:1000).

Techniques: Recombinant, Sequencing, Software

Journal: bioRxiv

Article Title: Surfaceome dynamics during neuronal development and synaptic plasticity reveal system-wide surfaceome reorganization independent of global proteostasis

doi: 10.1101/731083

Figure Lengend Snippet: a , chemical LTP paradigm. b-e , Boxplots of distributions of log2 surface abundances for replicates of indicated conditions. Boxes indicate median and percentiles (25 th and 75 th ). f , Volcano plot of statistical significance (y-axis) and surface abundance change (x-axis). Horizontal line is located at an adjusted p-value of 0.05. g, Proteins with significantly different surface abundances (fold-change > 1.5 and p < 0.05) after cLTP grouped into categories. Shape size indicates scaled −log10 adjusted p-value. Border color indicates fold-change directionality (green > 0, purple < 0). Edges represent string confidence > 0.7. h , Representative images of analysed primary dendrites before and after cLTP induction. Bars 5 μm. i, Quantification of mean fluorescent intensity of antibody signal on the primary dendrite surface using antibodies against GluN: n=63, Bai1: n=42, Adcy3: n=28 (CTRL) n=22 (cLTP) cells/group. Means + 95% CI presented. ** p<0.01, **** (p<0.0001) using a two-way T-test (Adcy3+Bai1) or Mann-Whitney test (GluN).

Article Snippet: Antibodies targeting the extracellular domains of ADGRB1 (ABR-021, Alomone), AMPARs (182 411, Synaptic Systems), and AC3 (AAR-043, Alomone) were diluted 1:100 in blocking solution containing 10% normal goat serum (NGS) and incubated with the cells for 60 minutes.

Techniques: MANN-WHITNEY