Journal: Oncology Research Featuring Preclinical and Clinical Cancer Therapeutics

Article Title: Inhibition of NF-κB Activity Enhances Sensitivity to Anticancer Drugs in Cholangiocarcinoma Cells

doi: 10.3727/096504015x14424348426071

Figure Lengend Snippet: Figure 2. Effect of DHMEQ on 5-FU-induced cell death of CCA cell lines. Cells were treated with 5-FU with or without DHMEQ for 48 h. Cells were stained with various markers, and the image acquisitions were taken in an IN Cell Analyzer 2000. (A) Cells with nuclear fragmentation were identified by the nuclei marker, Hoechst 33342. Dead cells were identified by the presence of FITC-annexin V and/or propidium iodide. (B) Quantitative analysis of dead cell number. Bars represent the mean and SD of triplicates. The data are a representative from two independent experiments. *p < 0.05: (a) versus control, (b) versus DHMEQ, (c) versus 1 µM 5-FU, (d) versus 10 µM 5-FU.

Article Snippet: Cells were incubated further at 37°C, 5% CO 2 for 48 h, and stained with H33342, PI, and annexin V (Molecular Probes, Eugene, OR, USA) diluted in culture medium for 30 min before image acquisition in an IN Cell Analyzer 2000 (GE Healthcare, UK).

Techniques: Staining, Marker, Control