Journal: PLoS ONE

Article Title: Abnormalities of AMPK Activation and Glucose Uptake in Cultured Skeletal Muscle Cells from Individuals with Chronic Fatigue Syndrome

doi: 10.1371/journal.pone.0122982

Figure Lengend Snippet: Representative light microscope images of 2 control (top panels) and 2 CFS (bottom panels) cultures taken 7 days after initiation of differentiation. Images were taken at a 10x magnification (A). MYOG expression at 24h, 72h and 7 days after initiation of differentiation from control and CFS cultures was measured by QPCR (B). Data were normalised to the reference gene, β2-microglobulin and are presented as the mean±SEM from 5 controls and 8 CFS subjects analysed in triplicate. ***p<0.0001. IL6 release into the media was measured at 24h, 72h and 7 days after initiation of differentiation from control and CFS cultures by ELISA (C). Data are expressed as the mean±SEM from 5 controls and 8 CFS subjects in duplicate. *p<0.05.

Article Snippet: IL6 ELISA kits were obtained from Qiagen (Sussex, UK).

Techniques: Light Microscopy, Control, Expressing, Enzyme-linked Immunosorbent Assay

Journal: PLoS ONE

Article Title: Abnormalities of AMPK Activation and Glucose Uptake in Cultured Skeletal Muscle Cells from Individuals with Chronic Fatigue Syndrome

doi: 10.1371/journal.pone.0122982

Figure Lengend Snippet: Fresh media was added to 5 controls and 10 CFS cultures and subjected to EPS for 4 and 24h. After 24h, media was removed and assayed for IL6 secretion by ELISA. Open bar: Unstimulated, closed bar: 4h stimulation, grey bar: 24h stimulation. Results are expressed as mean±SEM. # p<0.001 vs control unstimulated, * p<0.05 vs corresponding control.

Article Snippet: IL6 ELISA kits were obtained from Qiagen (Sussex, UK).

Techniques: Enzyme-linked Immunosorbent Assay, Control