Journal: Neuron

Article Title: Wireless Optogenetic Stimulation of Oxytocin Neurons in a Semi-natural Setup Dynamically Elevates Both Pro-social and Agonistic Behaviors

doi: 10.1016/j.neuron.2020.05.028

Figure Lengend Snippet:

Article Snippet: Ornithine Vasotocin Analog (OVTA) , Perkin Elmer , NEX254050UC.

Techniques: Virus, Recombinant, Software, Amplification, Adhesive

Journal: EMBO Reports

Article Title: Hippo pathway controls biopterin metabolism to shield adjacent cells from ferroptosis in lung cancer

doi: 10.1038/s44319-025-00515-4

Figure Lengend Snippet: ( A ) Actinomycin D-induced cell death was rescued by the apoptosis inhibitor Z-VAD-FMK. Wild-type (WT) LLC cells were pretreated (or not) with Z-VAD-FMK (50 µM) for 1 h, followed by stimulation with Actinomycin D (500 nM) for 24 h. Dead cells were stained with propidium iodide (PI), and the percentage of the PI-negative live cell population was calculated using the flow cytometer. Data are means ± SD of three biologically independent samples from a representative experiment. **** (a) P = 0.000000000000065; **** (b) P = 0.000000000000065 (one-way ANOVA test followed by Tukey’s multiple comparison test). ( B ) TCZ-induced cell death was rescued by the necroptosis inhibitor Necrostatin-1. WT LLC cells were pretreated (or not) with Necrostatin-1 for 1 h, followed by stimulation with TCZ [T, TNFα (30 ng/mL); C, cycloheximide (30 µg/mL); Z, Z-VAD-FMK (20 µM)] for 24 h. The percentage of live cell population was calculated using PI and flow cytometer. Data are means ± SD of three biologically independent samples from a representative experiment. **** (a) P = 0.000000012723845; **** (b) P = 0.000000720360306 (one-way ANOVA test followed by Tukey’s multiple comparison test). ( C ) RSL3-induced cell death was rescued by the ferroptosis inhibitor Ferrostatin-1. WT LLC cells were pretreated (or not) with Ferrostatin-1 (5 µM) for 30 min, followed by stimulation with RSL3 (400 nM) for 10 h. The percentage of live cell population was calculated using PI and flow cytometer. Data are means ± SD of three biologically independent samples from a representative experiment. **** (a) P = 0.000000048729652; **** (b) P = 0.000000056548852 (one-way ANOVA test followed by Tukey’s multiple comparison test). ( D ) YAP/TAZ loss sensitizes LLC cells to apoptosis. WT and YAP/TAZ dKO LLC cells were treated with Actinomycin D (500 nM) for 24 h, followed by cell viability assay using flow cytometer. Data are means ± SD of three biologically independent samples from a representative experiment. **** P = 0.000030539048461 (one-way ANOVA test followed by Tukey’s multiple comparison test). ( E ) YAP/TAZ loss sensitizes LLC cells to necroptosis. WT and YAP/TAZ dKO LLC cells were treated with TCZ [T, TNFα (30 ng/mL); C, cycloheximide (30 µg/mL); Z, Z-VAD-FMK (20 µM)] for 24 h, followed by cell viability assay using flow cytometer. Data are means ± SD of three biologically independent samples from a representative experiment. **** P = 0.000000000000054 (one-way ANOVA test followed by Tukey’s multiple comparison test). ( F ) YAP/TAZ loss confers resistance to ferroptosis in LLC cells. WT and YAP/TAZ dKO LLC cells were treated with RSL3 (400 nM) for 10 h, followed by cell viability assay using flow cytometer. Data are means ± SD of three biologically independent samples from a representative experiment. **** P = 0.000000000000051 (one-way ANOVA test followed by Tukey’s multiple comparison test). ( G ) Cell viability analysis was performed on WT and YAP/TAZ dKO LLC cells treated with indicated concentrations of RSL3 for 10 h, followed by cell viability assay using flow cytometer. Data are means ± SD of three biologically independent samples from a representative experiment. ( H ) Cell death induced by ferroptosis-inducing reagents or cysteine and cystine starvation (CCS) was rescued by the ferroptosis inhibitor Ferrostatin-1. WT LLC cells were pretreated (or not) with Ferrostatin-1 (5 µM) for 30 min, followed by stimulation with Erastin (5 µM) for 10 h, L-Buthionine-(S,R)-Sulfoximine (L-BSO; 10 mM) for 24 h, or Sulfasalazine (SAS; 1 mM) for 24 h. For CCS, cells were cultured in the cysteine and cystine-starved medium for 24 h. The percentage of live cell population was calculated using flow cytometer. Data are means ± SD of three biologically independent samples from a representative experiment. **** (a) P = 0.000000000005959; **** (b) P = 0.000000096903963; **** (c) P = 0.000000191080058; **** (d) P = 0.000000000000065 (one-way ANOVA test followed by Tukey’s multiple comparison test). ( I ) YAP/TAZ loss confers resistance to ferroptosis in LLC cells. WT and YAP/TAZ dKO LLC cells were treated with Erastin (5 µM) for 10 h, L-BSO (10 mM) for 24 h, or SAS (1 mM) for 24 h. For CCS, cells were cultured in the cysteine and cystine-starved medium for 24 h. The percentage of live cell population was calculated using flow cytometer. Data are means ± SD of three biologically independent samples from a representative experiment. **** (a) P = 0.000000000000769; **** (b) P = 0.000000000883037; **** (c) P = 0.000000000024749; **** (d) P = 0.000000000000051 (one-way ANOVA test followed by Tukey’s multiple comparison test).

Article Snippet: Cells were treated with the following reagents for the indicated time: (1S,3 R)-RSL3 (400 nM, #S8155; Selleck Chemicals, Houston, TX, USA), Erastin (5 μM, #17754; Cayman Chemical), L-Buthionine-(S,R)-Sulfoximine (L-BSO, 10 mM, #14484; Cayman Chemical), Sulfasalazine (SAS, 1 mM, #HY-14655; MedChemExpress), actinomycin D (500 nM, #018-21264; Wako), TNF-α (30 ng/mL, #ab206641; Abcam, Cambridge, UK), cycloheximide (30 μg/mL, #037-20991; Wako), Z-VAD-FMK (50 μM for apoptosis inhibition, 20 μM for necroptosis induction, #S7023; Selleck Chemicals), Necrostatin-1 (50 μM, #S8037; Selleck Chemicals), Ferrostatin-1 (5 μM, #17729; Cayman Chemical, Ann Arbor, MI, USA), (6 R)-5,6,7,8-tetrahydro-L-biopterin (BH4, 40 μM, #81880; Cayman Chemical), 7,8-dihydro-L-biopterin (BH2) (40 μM, #81882; Cayman Chemical), and DAHP (10 mM, #81260; Cayman Chemical).

Techniques: Staining, Flow Cytometry, Comparison, Viability Assay, Cell Culture

Journal: bioRxiv

Article Title: Evaluating the reliability of tools for mRNA annotation and IRES studies

doi: 10.64898/2026.03.29.707813

Figure Lengend Snippet: (A) A-cap / stemloop IRES reporter RNA carrying the negative control “scramble 1” sequence was mixed into a polysome lysate made from HEK293T cells for five minutes on ice and loaded on a sucrose gradient. (B) The sucrose gradient was fractionated and cDNA was prepared from groups of fractions as shown (numbered 1 -6). qRT-PCR was performed to quantify the exogenous “scramble 1” RNA across the fractions, as compared to endogenous Gapdh mRNA. The “scramble 1” transcript migrated into polysomal fractions 4-6, even though it is not translated .

Article Snippet: The ARCA m 7 G-cap analog (New England Biolabs; S1411) was used at a 4:1 ratio to GFP for transcription of T7-nluc constructs, while an A-cap analog (New England Biolabs S1406), was used to generate T7HP-nluc IRES reporter RNAs.

Techniques: Negative Control, Sequencing, Quantitative RT-PCR