analog Search Results


gtpγs non hydrolysable gtp analogue  (Cytoskeleton Inc)
92
Cytoskeleton Inc gtpγs non hydrolysable gtp analogue
Gtpγs Non Hydrolysable Gtp Analogue, supplied by Cytoskeleton Inc, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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rna gpppa  (New England Biolabs)
95
New England Biolabs rna gpppa
FP saturation binding and competitive displacement curves for the nsp10-nsp16 complex. ( A ) The nsp10-nsp16 complex binds to 5′ N7-meGpppACCCCC-FAM 3′ with a K d of 0.13 ± 0.002 µM and B max of 270 ± 5 mP. Unlabeled methylated cap RNAs, ( B ) N7-meGpppG and ( C ) N7-meGpppA, compete with 5′ N7-meGpppACCCCC-FAM 3′ for binding to the nsp10-nsp16 complex. Unlabeled unmethylated cap RNAs, ( D ) GpppG and ( E ) <t>GpppA,</t> did not disrupt the interaction between the nsp10-nsp16 complex and <t>RNA-FAM.</t> All values are the mean ± standard deviation of three independent experiments. ( F ) The Z′ factor (0.6) was determined for evaluation of the assay for high-throughput screening. FAM-labeled RNA (5′ N7-meGpppACCCCC-FAM 3′) at 30 nM was used to generate the FP signal, while 50 µM unlabeled RNA (N7-meGpppA) was used as a positive control. Data were analyzed using GraphPad Prism software 7.0.4.
Rna Gpppa, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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hoechst  (MedChemExpress)
93
MedChemExpress hoechst
FP saturation binding and competitive displacement curves for the nsp10-nsp16 complex. ( A ) The nsp10-nsp16 complex binds to 5′ N7-meGpppACCCCC-FAM 3′ with a K d of 0.13 ± 0.002 µM and B max of 270 ± 5 mP. Unlabeled methylated cap RNAs, ( B ) N7-meGpppG and ( C ) N7-meGpppA, compete with 5′ N7-meGpppACCCCC-FAM 3′ for binding to the nsp10-nsp16 complex. Unlabeled unmethylated cap RNAs, ( D ) GpppG and ( E ) <t>GpppA,</t> did not disrupt the interaction between the nsp10-nsp16 complex and <t>RNA-FAM.</t> All values are the mean ± standard deviation of three independent experiments. ( F ) The Z′ factor (0.6) was determined for evaluation of the assay for high-throughput screening. FAM-labeled RNA (5′ N7-meGpppACCCCC-FAM 3′) at 30 nM was used to generate the FP signal, while 50 µM unlabeled RNA (N7-meGpppA) was used as a positive control. Data were analyzed using GraphPad Prism software 7.0.4.
Hoechst, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 93 stars, based on 1 article reviews
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m7g 5 ppp 5  (New England Biolabs)
95
New England Biolabs m7g 5 ppp 5
FP saturation binding and competitive displacement curves for the nsp10-nsp16 complex. ( A ) The nsp10-nsp16 complex binds to 5′ N7-meGpppACCCCC-FAM 3′ with a K d of 0.13 ± 0.002 µM and B max of 270 ± 5 mP. Unlabeled methylated cap RNAs, ( B ) N7-meGpppG and ( C ) N7-meGpppA, compete with 5′ N7-meGpppACCCCC-FAM 3′ for binding to the nsp10-nsp16 complex. Unlabeled unmethylated cap RNAs, ( D ) GpppG and ( E ) <t>GpppA,</t> did not disrupt the interaction between the nsp10-nsp16 complex and <t>RNA-FAM.</t> All values are the mean ± standard deviation of three independent experiments. ( F ) The Z′ factor (0.6) was determined for evaluation of the assay for high-throughput screening. FAM-labeled RNA (5′ N7-meGpppACCCCC-FAM 3′) at 30 nM was used to generate the FP signal, while 50 µM unlabeled RNA (N7-meGpppA) was used as a positive control. Data were analyzed using GraphPad Prism software 7.0.4.
M7g 5 Ppp 5, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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medchemexpress llc  (MedChemExpress)
94
MedChemExpress medchemexpress llc
FP saturation binding and competitive displacement curves for the nsp10-nsp16 complex. ( A ) The nsp10-nsp16 complex binds to 5′ N7-meGpppACCCCC-FAM 3′ with a K d of 0.13 ± 0.002 µM and B max of 270 ± 5 mP. Unlabeled methylated cap RNAs, ( B ) N7-meGpppG and ( C ) N7-meGpppA, compete with 5′ N7-meGpppACCCCC-FAM 3′ for binding to the nsp10-nsp16 complex. Unlabeled unmethylated cap RNAs, ( D ) GpppG and ( E ) <t>GpppA,</t> did not disrupt the interaction between the nsp10-nsp16 complex and <t>RNA-FAM.</t> All values are the mean ± standard deviation of three independent experiments. ( F ) The Z′ factor (0.6) was determined for evaluation of the assay for high-throughput screening. FAM-labeled RNA (5′ N7-meGpppACCCCC-FAM 3′) at 30 nM was used to generate the FP signal, while 50 µM unlabeled RNA (N7-meGpppA) was used as a positive control. Data were analyzed using GraphPad Prism software 7.0.4.
Medchemexpress Llc, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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3q l azetidine 2 carboxylic acid 1m phosphate  (Chem Impex International)
96
Chem Impex International 3q l azetidine 2 carboxylic acid 1m phosphate
FP saturation binding and competitive displacement curves for the nsp10-nsp16 complex. ( A ) The nsp10-nsp16 complex binds to 5′ N7-meGpppACCCCC-FAM 3′ with a K d of 0.13 ± 0.002 µM and B max of 270 ± 5 mP. Unlabeled methylated cap RNAs, ( B ) N7-meGpppG and ( C ) N7-meGpppA, compete with 5′ N7-meGpppACCCCC-FAM 3′ for binding to the nsp10-nsp16 complex. Unlabeled unmethylated cap RNAs, ( D ) GpppG and ( E ) <t>GpppA,</t> did not disrupt the interaction between the nsp10-nsp16 complex and <t>RNA-FAM.</t> All values are the mean ± standard deviation of three independent experiments. ( F ) The Z′ factor (0.6) was determined for evaluation of the assay for high-throughput screening. FAM-labeled RNA (5′ N7-meGpppACCCCC-FAM 3′) at 30 nM was used to generate the FP signal, while 50 µM unlabeled RNA (N7-meGpppA) was used as a positive control. Data were analyzed using GraphPad Prism software 7.0.4.
3q L Azetidine 2 Carboxylic Acid 1m Phosphate, supplied by Chem Impex International, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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96/100 stars
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1 nm pp1  (TargetMol)
94
TargetMol 1 nm pp1
FP saturation binding and competitive displacement curves for the nsp10-nsp16 complex. ( A ) The nsp10-nsp16 complex binds to 5′ N7-meGpppACCCCC-FAM 3′ with a K d of 0.13 ± 0.002 µM and B max of 270 ± 5 mP. Unlabeled methylated cap RNAs, ( B ) N7-meGpppG and ( C ) N7-meGpppA, compete with 5′ N7-meGpppACCCCC-FAM 3′ for binding to the nsp10-nsp16 complex. Unlabeled unmethylated cap RNAs, ( D ) GpppG and ( E ) <t>GpppA,</t> did not disrupt the interaction between the nsp10-nsp16 complex and <t>RNA-FAM.</t> All values are the mean ± standard deviation of three independent experiments. ( F ) The Z′ factor (0.6) was determined for evaluation of the assay for high-throughput screening. FAM-labeled RNA (5′ N7-meGpppACCCCC-FAM 3′) at 30 nM was used to generate the FP signal, while 50 µM unlabeled RNA (N7-meGpppA) was used as a positive control. Data were analyzed using GraphPad Prism software 7.0.4.
1 Nm Pp1, supplied by TargetMol, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/1 nm pp1/product/TargetMol
Average 94 stars, based on 1 article reviews
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rna cap structure analog  (New England Biolabs)
94
New England Biolabs rna cap structure analog
A. METTL3/METTL14 enzyme titration. METTL3/METTL14 was titrated in the presence of 250 nM SAM and 1 <t>μM</t> <t>ssRNA</t> 28mer; reactions were incubated at 30 °C for 2 hr. B. SAH quantification. TR-FRET values were converted to SAH production using a standard curve. C. Dose response curves with probe inhibitors for METTL3/METTL14. Performed under initial velocity conditions as described for A, above. UZH2 and STM2457 showed IC 50 values of 16 nM and 17 nM, respectively. D. nsp14 enzyme titration. Nsp14 was titrated in the presence of 100 nM SAM and 50 μM <t>RNA</t> Cap; reactions were incubated at 37 °C for 90 min. E. SAH quantification. TR-FRET values were converted to SAH production using a standard curve. F. Z’ determination. Measured using initial velocity conditions: 1.45 nM nsp14, 90 min reaction.
Rna Cap Structure Analog, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 94 stars, based on 1 article reviews
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cap analog  (New England Biolabs)
96
New England Biolabs cap analog
A. METTL3/METTL14 enzyme titration. METTL3/METTL14 was titrated in the presence of 250 nM SAM and 1 <t>μM</t> <t>ssRNA</t> 28mer; reactions were incubated at 30 °C for 2 hr. B. SAH quantification. TR-FRET values were converted to SAH production using a standard curve. C. Dose response curves with probe inhibitors for METTL3/METTL14. Performed under initial velocity conditions as described for A, above. UZH2 and STM2457 showed IC 50 values of 16 nM and 17 nM, respectively. D. nsp14 enzyme titration. Nsp14 was titrated in the presence of 100 nM SAM and 50 μM <t>RNA</t> Cap; reactions were incubated at 37 °C for 90 min. E. SAH quantification. TR-FRET values were converted to SAH production using a standard curve. F. Z’ determination. Measured using initial velocity conditions: 1.45 nM nsp14, 90 min reaction.
Cap Analog, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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cap analog - by Bioz Stars, 2026-02
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3 0 me m7g 5 ppp 5 g rna cap structure analog  (New England Biolabs)
96
New England Biolabs 3 0 me m7g 5 ppp 5 g rna cap structure analog
A. METTL3/METTL14 enzyme titration. METTL3/METTL14 was titrated in the presence of 250 nM SAM and 1 <t>μM</t> <t>ssRNA</t> 28mer; reactions were incubated at 30 °C for 2 hr. B. SAH quantification. TR-FRET values were converted to SAH production using a standard curve. C. Dose response curves with probe inhibitors for METTL3/METTL14. Performed under initial velocity conditions as described for A, above. UZH2 and STM2457 showed IC 50 values of 16 nM and 17 nM, respectively. D. nsp14 enzyme titration. Nsp14 was titrated in the presence of 100 nM SAM and 50 μM <t>RNA</t> Cap; reactions were incubated at 37 °C for 90 min. E. SAH quantification. TR-FRET values were converted to SAH production using a standard curve. F. Z’ determination. Measured using initial velocity conditions: 1.45 nM nsp14, 90 min reaction.
3 0 Me M7g 5 Ppp 5 G Rna Cap Structure Analog, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/3 0 me m7g 5 ppp 5 g rna cap structure analog/product/New England Biolabs
Average 96 stars, based on 1 article reviews
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rna transcripts  (New England Biolabs)
94
New England Biolabs rna transcripts
A. METTL3/METTL14 enzyme titration. METTL3/METTL14 was titrated in the presence of 250 nM SAM and 1 <t>μM</t> <t>ssRNA</t> 28mer; reactions were incubated at 30 °C for 2 hr. B. SAH quantification. TR-FRET values were converted to SAH production using a standard curve. C. Dose response curves with probe inhibitors for METTL3/METTL14. Performed under initial velocity conditions as described for A, above. UZH2 and STM2457 showed IC 50 values of 16 nM and 17 nM, respectively. D. nsp14 enzyme titration. Nsp14 was titrated in the presence of 100 nM SAM and 50 μM <t>RNA</t> Cap; reactions were incubated at 37 °C for 90 min. E. SAH quantification. TR-FRET values were converted to SAH production using a standard curve. F. Z’ determination. Measured using initial velocity conditions: 1.45 nM nsp14, 90 min reaction.
Rna Transcripts, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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g 5 ppp 5 g rna cap structure analogue neb  (New England Biolabs)
95
New England Biolabs g 5 ppp 5 g rna cap structure analogue neb
A. METTL3/METTL14 enzyme titration. METTL3/METTL14 was titrated in the presence of 250 nM SAM and 1 <t>μM</t> <t>ssRNA</t> 28mer; reactions were incubated at 30 °C for 2 hr. B. SAH quantification. TR-FRET values were converted to SAH production using a standard curve. C. Dose response curves with probe inhibitors for METTL3/METTL14. Performed under initial velocity conditions as described for A, above. UZH2 and STM2457 showed IC 50 values of 16 nM and 17 nM, respectively. D. nsp14 enzyme titration. Nsp14 was titrated in the presence of 100 nM SAM and 50 μM <t>RNA</t> Cap; reactions were incubated at 37 °C for 90 min. E. SAH quantification. TR-FRET values were converted to SAH production using a standard curve. F. Z’ determination. Measured using initial velocity conditions: 1.45 nM nsp14, 90 min reaction.
G 5 Ppp 5 G Rna Cap Structure Analogue Neb, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 95 stars, based on 1 article reviews
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Image Search Results


FP saturation binding and competitive displacement curves for the nsp10-nsp16 complex. ( A ) The nsp10-nsp16 complex binds to 5′ N7-meGpppACCCCC-FAM 3′ with a K d of 0.13 ± 0.002 µM and B max of 270 ± 5 mP. Unlabeled methylated cap RNAs, ( B ) N7-meGpppG and ( C ) N7-meGpppA, compete with 5′ N7-meGpppACCCCC-FAM 3′ for binding to the nsp10-nsp16 complex. Unlabeled unmethylated cap RNAs, ( D ) GpppG and ( E ) GpppA, did not disrupt the interaction between the nsp10-nsp16 complex and RNA-FAM. All values are the mean ± standard deviation of three independent experiments. ( F ) The Z′ factor (0.6) was determined for evaluation of the assay for high-throughput screening. FAM-labeled RNA (5′ N7-meGpppACCCCC-FAM 3′) at 30 nM was used to generate the FP signal, while 50 µM unlabeled RNA (N7-meGpppA) was used as a positive control. Data were analyzed using GraphPad Prism software 7.0.4.

Journal: Slas Discovery

Article Title: A High-Throughput RNA Displacement Assay for Screening SARS-CoV-2 nsp10-nsp16 Complex toward Developing Therapeutics for COVID-19

doi: 10.1177/2472555220985040

Figure Lengend Snippet: FP saturation binding and competitive displacement curves for the nsp10-nsp16 complex. ( A ) The nsp10-nsp16 complex binds to 5′ N7-meGpppACCCCC-FAM 3′ with a K d of 0.13 ± 0.002 µM and B max of 270 ± 5 mP. Unlabeled methylated cap RNAs, ( B ) N7-meGpppG and ( C ) N7-meGpppA, compete with 5′ N7-meGpppACCCCC-FAM 3′ for binding to the nsp10-nsp16 complex. Unlabeled unmethylated cap RNAs, ( D ) GpppG and ( E ) GpppA, did not disrupt the interaction between the nsp10-nsp16 complex and RNA-FAM. All values are the mean ± standard deviation of three independent experiments. ( F ) The Z′ factor (0.6) was determined for evaluation of the assay for high-throughput screening. FAM-labeled RNA (5′ N7-meGpppACCCCC-FAM 3′) at 30 nM was used to generate the FP signal, while 50 µM unlabeled RNA (N7-meGpppA) was used as a positive control. Data were analyzed using GraphPad Prism software 7.0.4.

Article Snippet: The unlabeled Cap RNA, m7G(5′)ppp(5′G (NEB, cat. S1404S) and m7G(5′)ppp(5′)A (NEB, cat. S1405S), also referred to as N7-meGpppG and N7-meGpppA for consistency, respectively; G(5′)ppp(5′)A RNA (GpppA) (NEB, cat. S1406S); and G(5′)ppp(5′)G RNA (GpppG) (NEB, cat. S1407S) are from New England Biolabs (NEB, Whitby, Ontario, Canada).

Techniques: Binding Assay, Methylation, Standard Deviation, High Throughput Screening Assay, Labeling, Positive Control, Software

A. METTL3/METTL14 enzyme titration. METTL3/METTL14 was titrated in the presence of 250 nM SAM and 1 μM ssRNA 28mer; reactions were incubated at 30 °C for 2 hr. B. SAH quantification. TR-FRET values were converted to SAH production using a standard curve. C. Dose response curves with probe inhibitors for METTL3/METTL14. Performed under initial velocity conditions as described for A, above. UZH2 and STM2457 showed IC 50 values of 16 nM and 17 nM, respectively. D. nsp14 enzyme titration. Nsp14 was titrated in the presence of 100 nM SAM and 50 μM RNA Cap; reactions were incubated at 37 °C for 90 min. E. SAH quantification. TR-FRET values were converted to SAH production using a standard curve. F. Z’ determination. Measured using initial velocity conditions: 1.45 nM nsp14, 90 min reaction.

Journal: SLAS discovery : advancing life sciences R & D

Article Title: Development and validation of a generic methyltransferase enzymatic assay based on an SAH riboswitch

doi: 10.1016/j.slasd.2024.100161

Figure Lengend Snippet: A. METTL3/METTL14 enzyme titration. METTL3/METTL14 was titrated in the presence of 250 nM SAM and 1 μM ssRNA 28mer; reactions were incubated at 30 °C for 2 hr. B. SAH quantification. TR-FRET values were converted to SAH production using a standard curve. C. Dose response curves with probe inhibitors for METTL3/METTL14. Performed under initial velocity conditions as described for A, above. UZH2 and STM2457 showed IC 50 values of 16 nM and 17 nM, respectively. D. nsp14 enzyme titration. Nsp14 was titrated in the presence of 100 nM SAM and 50 μM RNA Cap; reactions were incubated at 37 °C for 90 min. E. SAH quantification. TR-FRET values were converted to SAH production using a standard curve. F. Z’ determination. Measured using initial velocity conditions: 1.45 nM nsp14, 90 min reaction.

Article Snippet: G(5′)ppp(5′)A RNA Cap Structure Analog was from New England BioLabs Inc. (Cat. #S1406L). ssRNA Oligomer (5′-GUUGCCUGUUCGUGUUGGACUUGCCUGU-3′) was from IDT.

Techniques: Titration, Incubation