Journal: Archives of Razi Institute

Article Title: Prevalence of Anti-Human Herpes Virus Type 7 IgG Positivity Rate among Children with Fever and Skin Rash in Diyala Province, Iraq

doi: 10.22092/ARI.2022.359149.2381

Figure Lengend Snippet: Distribution of anti-HHV-7 IgM and IgG in study groups

Article Snippet: Enzyme-linked immunosorbent assay (ELISA) kits for the detection of anti-HHV-7 IgG were used (Mybiosource-China).

Techniques: Control, Significance Assay, Virus

Journal: Archives of Razi Institute

Article Title: Prevalence of Anti-Human Herpes Virus Type 7 IgG Positivity Rate among Children with Fever and Skin Rash in Diyala Province, Iraq

doi: 10.22092/ARI.2022.359149.2381

Figure Lengend Snippet: Association of anti-HHV-7 IgG positivity with CBCs in study groups

Article Snippet: Enzyme-linked immunosorbent assay (ELISA) kits for the detection of anti-HHV-7 IgG were used (Mybiosource-China).

Techniques: Virus

Journal: Frontiers in Immunology

Article Title: A Kinetic Study of CD83 Reveals an Upregulation and Higher Production of sCD83 in Lymphocytes from Pregnant Mice

doi: 10.3389/fimmu.2017.00486

Figure Lengend Snippet: B cells (B220 + ), T cells (CD4 + ), and dendritic cells (33D1 + ) from fresh spleen tissue were examined in the course of normal murine pregnancies . (A) Representative plots showing gating strategies to CD83 expression (right). (B) Scatter dot plots show the median fluorescence intensity of CD83 within the respective cell types in live lymphocytes. (C) B220 + B cells were further subdivided in CD21/35 hi CD23 low marginal zone B cells (MZ), CD21/35 int CD23 hi follicular zone B cells (FO), and CD21/35 − CD23 − transitional B cells (TN). Scatter dot plots show the mean fluorescence intensity of CD83. (D) Representative histogram overlapping CD83 expression on B cells of a non-pregnant mice (black line) or pregnant mice at day 14 (gray line). (E) Magnetic isolated splenic CD19 + B cells were analyzed for mRNA expression levels of CD83 by quantitative real-time PCR. FACS data were analyzed by Kruskal–Wallis test with Dunn’s posttest. PCR was analyzed by Student’s t -test. Significant differences are indicated (* p ≤ 0.05, ** p ≤ 0.01). (F) Data show the percentage of CD83 + cells within the respective cell types in spleen. T cells were further characterized within the CD25 + population. Data show the percentage of CD83 + cells within CD25 + T cells. Data were analyzed by ANOVA with Tuckey’s posttest. Significant differences are indicated (* p ≤ 0.05, *** p ≤ 0.001).

Article Snippet: The levels of sCD83 in sera and supernatants were measured using an ELISA kit for CD83 (Cloud-Clone Corp., Houston, TX, USA).

Techniques: Expressing, Fluorescence, Isolation, Real-time Polymerase Chain Reaction

Journal: Frontiers in Immunology

Article Title: A Kinetic Study of CD83 Reveals an Upregulation and Higher Production of sCD83 in Lymphocytes from Pregnant Mice

doi: 10.3389/fimmu.2017.00486

Figure Lengend Snippet: B cells (B220 + ), T cells (CD4 + ), and dendritic cells (33D1 + ) from uterine-draining lymph nodes were examined in the course of pregnancy . (A) Scatter dot plots show the mean fluorescence intensity of CD83 within the respective cell types in paraaortic lymph nodes. (B) Scatter dot plots show the mean fluorescence intensity of CD83 within the respective cell types in inguinal lymph nodes. Data were analyzed by Kruskal–Wallis test with Dunn’s posttest. Significant differences are indicated (* p ≤ 0.05). (C) Percentage of CD83 + cells within the respective cell types in paraaortic lymph nodes (C) and inguinal lymph nodes (D) . Data were analyzed by ANOVA with Tuckey’s posttest. Significant differences are indicated (* p ≤ 0.05, ** p ≤ 0.01). (E) Sera of pregnant and non-pregnant mice were analyzed by ELISA to determine sCD83 levels during pregnancy. Data were analyzed by Kruskal–Wallis test with Dunn’s posttest.

Article Snippet: The levels of sCD83 in sera and supernatants were measured using an ELISA kit for CD83 (Cloud-Clone Corp., Houston, TX, USA).

Techniques: Fluorescence, Enzyme-linked Immunosorbent Assay

Journal: Frontiers in Immunology

Article Title: A Kinetic Study of CD83 Reveals an Upregulation and Higher Production of sCD83 in Lymphocytes from Pregnant Mice

doi: 10.3389/fimmu.2017.00486

Figure Lengend Snippet: Splenic lymphocytes from different stages of murine pregnancy were treated with key pregnancy hormones, progesterone and estrogen in increasing concentrations for 48 h . Cells cultured in medium alone served as control. Scatter dot plots show the CD83 expression on CD19 + B cells, CD4 + T cells. and CD11c + dendritic cells after progesterone (A) or estrogen (B) stimulation. Data were analyzed by Kruskal–Wallis test with Dunn’s posttest. Significant differences are indicated (** p ≤ 0.01, *** p ≤ 0.001). (C) Splenic lymphocytes from different stages of murine pregnancy were stimulated with LPS for 48 h plus PMA and ionomycin for the last 5 h. The histogram shows a representative stimulation from a non-pregnant mouse B cells (left) and a pregnant mouse at day 18 (right). Gray curves show basal CD83 expression, the black line represents CD83 expression after stimulation. Scatter dot plots show the quotient between CD83 MFI of LPS-stimulated and nonstimulated CD19 + B cells (left) and CD4 + T cells (right). (D) mRNA expression levels of CD83 by quantitative real-time PCR of stimulated lymphocytes relative to nonstimulated controls. (E) Bars compare the amount of sCD83 in the supernatant from LPS-stimulated lymphocytes from non-pregnant and pregnant mice from late-pregnancy stage and nonstimulated controls. Data were analyzed by two-way-ANOVA with Bonferroni posttests (C) . Significant differences are indicated (* p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.001). (F) CD19- or CD4-depleted splenic lymphocytes and magnetic isolated splenic CD19 + B cells from mice at advanced pregnancies were cultured for 48 h. Data show the basal production of sCD83 in the supernatants in nonstimulated lymphocytes (F) or after stimulation with LPS, PMA, and ionomycin (G) . (H) Overlapping histogram display differences between nonstimulated (left) and stimulated (right) B cells (top) and CD4 + T cells (bottom). Filled curved represents the FMO for CD83, while filled and dashed lines show extracellular and intracellular CD83 staining, respectively. Data were analyzed with ANOVA and Dunnett’s test to compare treatments against non-depleted controls. Significant differences are indicated (* p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.001).

Article Snippet: The levels of sCD83 in sera and supernatants were measured using an ELISA kit for CD83 (Cloud-Clone Corp., Houston, TX, USA).

Techniques: Cell Culture, Expressing, Real-time Polymerase Chain Reaction, Isolation, Staining

Journal: Frontiers in Immunology

Article Title: A Kinetic Study of CD83 Reveals an Upregulation and Higher Production of sCD83 in Lymphocytes from Pregnant Mice

doi: 10.3389/fimmu.2017.00486

Figure Lengend Snippet: Effects of sCD83 on T cells . (A) CD4 + T cells were isolated from lymph nodes and cultured 24 h with supernatant obtained from the experiments on (Figure C) and treated with a blocking antibody against CD83 or an isotype control. (B) CD4 + T cells were isolated from lymph nodes and cultured 24 h with LPS, PMA, ionomycin, and either recombinant sCD83 or a control protein in similar concentrations. The cells were later analyzed for expression of activation markers and associated intracellular and surface markers. Student’s t -test was used to compare differences between the groups.

Article Snippet: The levels of sCD83 in sera and supernatants were measured using an ELISA kit for CD83 (Cloud-Clone Corp., Houston, TX, USA).

Techniques: Isolation, Cell Culture, Blocking Assay, Recombinant, Expressing, Activation Assay

Journal: Scientific Reports

Article Title: Increased cerebrospinal fluid fibrinogen in major depressive disorder

doi: 10.1038/srep11412

Figure Lengend Snippet: ( a ) Correlation between CSF fibrinogen (ELISA) and SL000022 (SOMAscan) levels (N = 32). ( b ) CSF fibrinogen levels in the second sample. ( c ) Plasma fibrinogen levels in plasma sample. Plain and dotted lines in ( b ) and ( c ) indicate the mean in each group and 99 percentile of the controls, respectively. CSF, cerebrospinal fluid; RFU, relative fluorescent unit; r, Pearson’s correlation coefficient; ELISA, enzyme-linked immunosorbent assay; CONT, control; MDD, major depressive disorder.

Article Snippet: An ELISA kit for fibrinogen gamma (SEC477Hu) was purchased from Uscn Life Science Inc. (Wuhan, China) and CSF samples were diluted to 1:10 using phosphate buffered saline.

Techniques: Enzyme-linked Immunosorbent Assay, Clinical Proteomics, Control