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Image Search Results
Journal: bioRxiv
Article Title: Post-Transcriptional Methylation of Mitochondrial-tRNA Differentially Contributes to Mitochondrial Pathology
doi: 10.1101/2023.12.09.569632
Figure Lengend Snippet: (Related to ) Design of primer sequences to quantify modifications in human mt-Leu(UAA) by primer extension. (A) The targeted sequence (underlined in blue) in human mt-Leu(UAA) by primer extension to quantify the level of m 1 G9 in a reaction with dCTP, ddATP, and Luna RT. (B) Denaturing gel analysis of the Luna RT stop on the transcript of mt-Leu(UAA) harboring m 1 G9 at 0, 25, 50, and 100%, showing the % of the RT stop relative to the readthrough to U8. (C) A linear correlation between the measured RT stop in (B) and the actual fraction of m 1 G9 in the transcript of mt-Leu(UAA) as prepared. (D) The targeted sequence (underlined in blue) in human mt-Leu(UAA) by primer extension to quantify the level of m 2 G10 in a reaction with dGTP, ddCTP, and SSIII. (E) The targeted sequence (underlined in blue) in human mt-Leu(UAA) by primer extension to quantify the level of m 1 A58 in a reaction with dATP, ddTTP, and SSIII. (F) The targeted sequence (underlined in blue) in human mt-Leu(UAA) by primer extension to quantify the level of 1m 5 U34 in a reaction with dTTP, dATP, ddGTP, and AMV RT.
Article Snippet:
Techniques: Sequencing