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Image Search Results
Journal: PLoS ONE
Article Title: Near-Infrared Laser Adjuvant for Influenza Vaccine
doi: 10.1371/journal.pone.0082899
Figure Lengend Snippet: A, Serum ovalbumin- (OVA)-specific IgG titers 3, 6, and 12 weeks following vaccination with 40 µg OVA with or without laser illumination. Endpoint titer of OVA-specific serum IgG was determined by ELISA. Plates were coated with OVA. n = 33, 19, 15, 12 and 11, 5 and 6, 6 and 6 for no OVA, OVA i.d., OVA +Alum i.d., OVA i.d. + PW 532 nm 1 and 4 minutes, OVA i.d. + PW 1064 nm 1 and 4 minutes, OVA i.d. + CW 1064 nm 1 and 4 minutes, respectively. Error bars show means ± s.e.m. * P <0.05, ** P <0.01 and *** P <0.001 as compared to OVA i.d. B, The relationships between anti-OVA antibody titers following 1–4 minutes PW 532 nm, PW 1064 nm and CW 1064 nm laser-treated groups at 6 weeks (logIgG) and maximal skin surface temperature (Tm) was not statistically significant; a Pearson's correlation coefficient r = −0.237 ( P = 0.08), where log(IgG) = −0.193Tm+10.75 (linear regression, R 2 = 0.056). n = 20, 17, 18, for OVA i.d. + PW 532 nm 1–4 minutes, OVA i.d. + PW 1064 nm 1–4 minutes, OVA i.d. + CW 1064 nm 1–4 minutes.
Article Snippet: In the case of IgE, the wells were further treated with
Techniques: Enzyme-linked Immunosorbent Assay
Journal: PLoS ONE
Article Title: Near-Infrared Laser Adjuvant for Influenza Vaccine
doi: 10.1371/journal.pone.0082899
Figure Lengend Snippet: A–G, Influenza-specific IgG subclass titers (A–C) in pre-challenge serum (4 weeks after vaccination) and (D–G) post-challenge (4 days after challenge). Mice were vaccinated with 1 µg of inactivated influenza virus (A/PR/8/34) with or without laser illumination or the licensed chemical adjuvant (alum) and challenged intranasally with live homologous virus 4 weeks after vaccination. Titer of influenza-specific serum IgG subclass was determined by ELISA. Plates were coated with inactivated influenza virus. (A and D) IgG, (B and E) IgG1, (C and F) IgG2c, and (G) IgE titers. Experimental and control groups: (A–C) n = 38, 47, 25, 50, 12 (D–F) n = 25, 29, 12, 32, 12 (G) n = 24, 21, 13, 24, 12 for no vaccine, vaccine i.d., vaccine i.d. + PW 532 nm, vaccine i.d. + CW 1064 nm, and vaccine + Alum i.d. vaccine groups, respectively.
Article Snippet: In the case of IgE, the wells were further treated with
Techniques: Virus, Adjuvant, Enzyme-linked Immunosorbent Assay, Control