amplification Search Results


91
Revvity elast elisa amplification system
A, Serum ovalbumin- (OVA)-specific IgG titers 3, 6, and 12 weeks following vaccination with 40 µg OVA with or without laser illumination. Endpoint titer of OVA-specific serum IgG was determined by <t>ELISA.</t> Plates were coated with OVA. n = 33, 19, 15, 12 and 11, 5 and 6, 6 and 6 for no OVA, OVA i.d., OVA +Alum i.d., OVA i.d. + PW 532 nm 1 and 4 minutes, OVA i.d. + PW 1064 nm 1 and 4 minutes, OVA i.d. + CW 1064 nm 1 and 4 minutes, respectively. Error bars show means ± s.e.m. * P <0.05, ** P <0.01 and *** P <0.001 as compared to OVA i.d. B, The relationships between anti-OVA antibody titers following 1–4 minutes PW 532 nm, PW 1064 nm and CW 1064 nm laser-treated groups at 6 weeks (logIgG) and maximal skin surface temperature (Tm) was not statistically significant; a Pearson's correlation coefficient r = −0.237 ( P = 0.08), where log(IgG) = −0.193Tm+10.75 (linear regression, R 2 = 0.056). n = 20, 17, 18, for OVA i.d. + PW 532 nm 1–4 minutes, OVA i.d. + PW 1064 nm 1–4 minutes, OVA i.d. + CW 1064 nm 1–4 minutes.
Elast Elisa Amplification System, supplied by Revvity, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/elast elisa amplification system/product/Revvity
Average 91 stars, based on 1 article reviews
elast elisa amplification system - by Bioz Stars, 2026-04
91/100 stars
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97
Roche kapa hyper prep kit
A, Serum ovalbumin- (OVA)-specific IgG titers 3, 6, and 12 weeks following vaccination with 40 µg OVA with or without laser illumination. Endpoint titer of OVA-specific serum IgG was determined by <t>ELISA.</t> Plates were coated with OVA. n = 33, 19, 15, 12 and 11, 5 and 6, 6 and 6 for no OVA, OVA i.d., OVA +Alum i.d., OVA i.d. + PW 532 nm 1 and 4 minutes, OVA i.d. + PW 1064 nm 1 and 4 minutes, OVA i.d. + CW 1064 nm 1 and 4 minutes, respectively. Error bars show means ± s.e.m. * P <0.05, ** P <0.01 and *** P <0.001 as compared to OVA i.d. B, The relationships between anti-OVA antibody titers following 1–4 minutes PW 532 nm, PW 1064 nm and CW 1064 nm laser-treated groups at 6 weeks (logIgG) and maximal skin surface temperature (Tm) was not statistically significant; a Pearson's correlation coefficient r = −0.237 ( P = 0.08), where log(IgG) = −0.193Tm+10.75 (linear regression, R 2 = 0.056). n = 20, 17, 18, for OVA i.d. + PW 532 nm 1–4 minutes, OVA i.d. + PW 1064 nm 1–4 minutes, OVA i.d. + CW 1064 nm 1–4 minutes.
Kapa Hyper Prep Kit, supplied by Roche, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/kapa hyper prep kit/product/Roche
Average 97 stars, based on 1 article reviews
kapa hyper prep kit - by Bioz Stars, 2026-04
97/100 stars
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96
New England Biolabs isothermal amplification buffer
A, Serum ovalbumin- (OVA)-specific IgG titers 3, 6, and 12 weeks following vaccination with 40 µg OVA with or without laser illumination. Endpoint titer of OVA-specific serum IgG was determined by <t>ELISA.</t> Plates were coated with OVA. n = 33, 19, 15, 12 and 11, 5 and 6, 6 and 6 for no OVA, OVA i.d., OVA +Alum i.d., OVA i.d. + PW 532 nm 1 and 4 minutes, OVA i.d. + PW 1064 nm 1 and 4 minutes, OVA i.d. + CW 1064 nm 1 and 4 minutes, respectively. Error bars show means ± s.e.m. * P <0.05, ** P <0.01 and *** P <0.001 as compared to OVA i.d. B, The relationships between anti-OVA antibody titers following 1–4 minutes PW 532 nm, PW 1064 nm and CW 1064 nm laser-treated groups at 6 weeks (logIgG) and maximal skin surface temperature (Tm) was not statistically significant; a Pearson's correlation coefficient r = −0.237 ( P = 0.08), where log(IgG) = −0.193Tm+10.75 (linear regression, R 2 = 0.056). n = 20, 17, 18, for OVA i.d. + PW 532 nm 1–4 minutes, OVA i.d. + PW 1064 nm 1–4 minutes, OVA i.d. + CW 1064 nm 1–4 minutes.
Isothermal Amplification Buffer, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/isothermal amplification buffer/product/New England Biolabs
Average 96 stars, based on 1 article reviews
isothermal amplification buffer - by Bioz Stars, 2026-04
96/100 stars
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96
Illumina Inc sureplex dna amplification system
A, Serum ovalbumin- (OVA)-specific IgG titers 3, 6, and 12 weeks following vaccination with 40 µg OVA with or without laser illumination. Endpoint titer of OVA-specific serum IgG was determined by <t>ELISA.</t> Plates were coated with OVA. n = 33, 19, 15, 12 and 11, 5 and 6, 6 and 6 for no OVA, OVA i.d., OVA +Alum i.d., OVA i.d. + PW 532 nm 1 and 4 minutes, OVA i.d. + PW 1064 nm 1 and 4 minutes, OVA i.d. + CW 1064 nm 1 and 4 minutes, respectively. Error bars show means ± s.e.m. * P <0.05, ** P <0.01 and *** P <0.001 as compared to OVA i.d. B, The relationships between anti-OVA antibody titers following 1–4 minutes PW 532 nm, PW 1064 nm and CW 1064 nm laser-treated groups at 6 weeks (logIgG) and maximal skin surface temperature (Tm) was not statistically significant; a Pearson's correlation coefficient r = −0.237 ( P = 0.08), where log(IgG) = −0.193Tm+10.75 (linear regression, R 2 = 0.056). n = 20, 17, 18, for OVA i.d. + PW 532 nm 1–4 minutes, OVA i.d. + PW 1064 nm 1–4 minutes, OVA i.d. + CW 1064 nm 1–4 minutes.
Sureplex Dna Amplification System, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/sureplex dna amplification system/product/Illumina Inc
Average 96 stars, based on 1 article reviews
sureplex dna amplification system - by Bioz Stars, 2026-04
96/100 stars
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97
Roche kapa htp library preparation kit
A, Serum ovalbumin- (OVA)-specific IgG titers 3, 6, and 12 weeks following vaccination with 40 µg OVA with or without laser illumination. Endpoint titer of OVA-specific serum IgG was determined by <t>ELISA.</t> Plates were coated with OVA. n = 33, 19, 15, 12 and 11, 5 and 6, 6 and 6 for no OVA, OVA i.d., OVA +Alum i.d., OVA i.d. + PW 532 nm 1 and 4 minutes, OVA i.d. + PW 1064 nm 1 and 4 minutes, OVA i.d. + CW 1064 nm 1 and 4 minutes, respectively. Error bars show means ± s.e.m. * P <0.05, ** P <0.01 and *** P <0.001 as compared to OVA i.d. B, The relationships between anti-OVA antibody titers following 1–4 minutes PW 532 nm, PW 1064 nm and CW 1064 nm laser-treated groups at 6 weeks (logIgG) and maximal skin surface temperature (Tm) was not statistically significant; a Pearson's correlation coefficient r = −0.237 ( P = 0.08), where log(IgG) = −0.193Tm+10.75 (linear regression, R 2 = 0.056). n = 20, 17, 18, for OVA i.d. + PW 532 nm 1–4 minutes, OVA i.d. + PW 1064 nm 1–4 minutes, OVA i.d. + CW 1064 nm 1–4 minutes.
Kapa Htp Library Preparation Kit, supplied by Roche, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/kapa htp library preparation kit/product/Roche
Average 97 stars, based on 1 article reviews
kapa htp library preparation kit - by Bioz Stars, 2026-04
97/100 stars
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96
Danaher Inc illustra genomiphi v2 dna amplification kit
A, Serum ovalbumin- (OVA)-specific IgG titers 3, 6, and 12 weeks following vaccination with 40 µg OVA with or without laser illumination. Endpoint titer of OVA-specific serum IgG was determined by <t>ELISA.</t> Plates were coated with OVA. n = 33, 19, 15, 12 and 11, 5 and 6, 6 and 6 for no OVA, OVA i.d., OVA +Alum i.d., OVA i.d. + PW 532 nm 1 and 4 minutes, OVA i.d. + PW 1064 nm 1 and 4 minutes, OVA i.d. + CW 1064 nm 1 and 4 minutes, respectively. Error bars show means ± s.e.m. * P <0.05, ** P <0.01 and *** P <0.001 as compared to OVA i.d. B, The relationships between anti-OVA antibody titers following 1–4 minutes PW 532 nm, PW 1064 nm and CW 1064 nm laser-treated groups at 6 weeks (logIgG) and maximal skin surface temperature (Tm) was not statistically significant; a Pearson's correlation coefficient r = −0.237 ( P = 0.08), where log(IgG) = −0.193Tm+10.75 (linear regression, R 2 = 0.056). n = 20, 17, 18, for OVA i.d. + PW 532 nm 1–4 minutes, OVA i.d. + PW 1064 nm 1–4 minutes, OVA i.d. + CW 1064 nm 1–4 minutes.
Illustra Genomiphi V2 Dna Amplification Kit, supplied by Danaher Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/illustra genomiphi v2 dna amplification kit/product/Danaher Inc
Average 96 stars, based on 1 article reviews
illustra genomiphi v2 dna amplification kit - by Bioz Stars, 2026-04
96/100 stars
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96
Roche kapa hifi hotstart readymix pcr kit
A, Serum ovalbumin- (OVA)-specific IgG titers 3, 6, and 12 weeks following vaccination with 40 µg OVA with or without laser illumination. Endpoint titer of OVA-specific serum IgG was determined by <t>ELISA.</t> Plates were coated with OVA. n = 33, 19, 15, 12 and 11, 5 and 6, 6 and 6 for no OVA, OVA i.d., OVA +Alum i.d., OVA i.d. + PW 532 nm 1 and 4 minutes, OVA i.d. + PW 1064 nm 1 and 4 minutes, OVA i.d. + CW 1064 nm 1 and 4 minutes, respectively. Error bars show means ± s.e.m. * P <0.05, ** P <0.01 and *** P <0.001 as compared to OVA i.d. B, The relationships between anti-OVA antibody titers following 1–4 minutes PW 532 nm, PW 1064 nm and CW 1064 nm laser-treated groups at 6 weeks (logIgG) and maximal skin surface temperature (Tm) was not statistically significant; a Pearson's correlation coefficient r = −0.237 ( P = 0.08), where log(IgG) = −0.193Tm+10.75 (linear regression, R 2 = 0.056). n = 20, 17, 18, for OVA i.d. + PW 532 nm 1–4 minutes, OVA i.d. + PW 1064 nm 1–4 minutes, OVA i.d. + CW 1064 nm 1–4 minutes.
Kapa Hifi Hotstart Readymix Pcr Kit, supplied by Roche, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/kapa hifi hotstart readymix pcr kit/product/Roche
Average 96 stars, based on 1 article reviews
kapa hifi hotstart readymix pcr kit - by Bioz Stars, 2026-04
96/100 stars
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97
Roche low throughput library preparation kit
A, Serum ovalbumin- (OVA)-specific IgG titers 3, 6, and 12 weeks following vaccination with 40 µg OVA with or without laser illumination. Endpoint titer of OVA-specific serum IgG was determined by <t>ELISA.</t> Plates were coated with OVA. n = 33, 19, 15, 12 and 11, 5 and 6, 6 and 6 for no OVA, OVA i.d., OVA +Alum i.d., OVA i.d. + PW 532 nm 1 and 4 minutes, OVA i.d. + PW 1064 nm 1 and 4 minutes, OVA i.d. + CW 1064 nm 1 and 4 minutes, respectively. Error bars show means ± s.e.m. * P <0.05, ** P <0.01 and *** P <0.001 as compared to OVA i.d. B, The relationships between anti-OVA antibody titers following 1–4 minutes PW 532 nm, PW 1064 nm and CW 1064 nm laser-treated groups at 6 weeks (logIgG) and maximal skin surface temperature (Tm) was not statistically significant; a Pearson's correlation coefficient r = −0.237 ( P = 0.08), where log(IgG) = −0.193Tm+10.75 (linear regression, R 2 = 0.056). n = 20, 17, 18, for OVA i.d. + PW 532 nm 1–4 minutes, OVA i.d. + PW 1064 nm 1–4 minutes, OVA i.d. + CW 1064 nm 1–4 minutes.
Low Throughput Library Preparation Kit, supplied by Roche, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/low throughput library preparation kit/product/Roche
Average 97 stars, based on 1 article reviews
low throughput library preparation kit - by Bioz Stars, 2026-04
97/100 stars
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96
Roche kapa hifi hotstart ready mix kit
A, Serum ovalbumin- (OVA)-specific IgG titers 3, 6, and 12 weeks following vaccination with 40 µg OVA with or without laser illumination. Endpoint titer of OVA-specific serum IgG was determined by <t>ELISA.</t> Plates were coated with OVA. n = 33, 19, 15, 12 and 11, 5 and 6, 6 and 6 for no OVA, OVA i.d., OVA +Alum i.d., OVA i.d. + PW 532 nm 1 and 4 minutes, OVA i.d. + PW 1064 nm 1 and 4 minutes, OVA i.d. + CW 1064 nm 1 and 4 minutes, respectively. Error bars show means ± s.e.m. * P <0.05, ** P <0.01 and *** P <0.001 as compared to OVA i.d. B, The relationships between anti-OVA antibody titers following 1–4 minutes PW 532 nm, PW 1064 nm and CW 1064 nm laser-treated groups at 6 weeks (logIgG) and maximal skin surface temperature (Tm) was not statistically significant; a Pearson's correlation coefficient r = −0.237 ( P = 0.08), where log(IgG) = −0.193Tm+10.75 (linear regression, R 2 = 0.056). n = 20, 17, 18, for OVA i.d. + PW 532 nm 1–4 minutes, OVA i.d. + PW 1064 nm 1–4 minutes, OVA i.d. + CW 1064 nm 1–4 minutes.
Kapa Hifi Hotstart Ready Mix Kit, supplied by Roche, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/kapa hifi hotstart ready mix kit/product/Roche
Average 96 stars, based on 1 article reviews
kapa hifi hotstart ready mix kit - by Bioz Stars, 2026-04
96/100 stars
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96
Danaher Inc illustra templiphi amplification kit
A, Serum ovalbumin- (OVA)-specific IgG titers 3, 6, and 12 weeks following vaccination with 40 µg OVA with or without laser illumination. Endpoint titer of OVA-specific serum IgG was determined by <t>ELISA.</t> Plates were coated with OVA. n = 33, 19, 15, 12 and 11, 5 and 6, 6 and 6 for no OVA, OVA i.d., OVA +Alum i.d., OVA i.d. + PW 532 nm 1 and 4 minutes, OVA i.d. + PW 1064 nm 1 and 4 minutes, OVA i.d. + CW 1064 nm 1 and 4 minutes, respectively. Error bars show means ± s.e.m. * P <0.05, ** P <0.01 and *** P <0.001 as compared to OVA i.d. B, The relationships between anti-OVA antibody titers following 1–4 minutes PW 532 nm, PW 1064 nm and CW 1064 nm laser-treated groups at 6 weeks (logIgG) and maximal skin surface temperature (Tm) was not statistically significant; a Pearson's correlation coefficient r = −0.237 ( P = 0.08), where log(IgG) = −0.193Tm+10.75 (linear regression, R 2 = 0.056). n = 20, 17, 18, for OVA i.d. + PW 532 nm 1–4 minutes, OVA i.d. + PW 1064 nm 1–4 minutes, OVA i.d. + CW 1064 nm 1–4 minutes.
Illustra Templiphi Amplification Kit, supplied by Danaher Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/illustra templiphi amplification kit/product/Danaher Inc
Average 96 stars, based on 1 article reviews
illustra templiphi amplification kit - by Bioz Stars, 2026-04
96/100 stars
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96
Roche kapa library amplification kits
A, Serum ovalbumin- (OVA)-specific IgG titers 3, 6, and 12 weeks following vaccination with 40 µg OVA with or without laser illumination. Endpoint titer of OVA-specific serum IgG was determined by <t>ELISA.</t> Plates were coated with OVA. n = 33, 19, 15, 12 and 11, 5 and 6, 6 and 6 for no OVA, OVA i.d., OVA +Alum i.d., OVA i.d. + PW 532 nm 1 and 4 minutes, OVA i.d. + PW 1064 nm 1 and 4 minutes, OVA i.d. + CW 1064 nm 1 and 4 minutes, respectively. Error bars show means ± s.e.m. * P <0.05, ** P <0.01 and *** P <0.001 as compared to OVA i.d. B, The relationships between anti-OVA antibody titers following 1–4 minutes PW 532 nm, PW 1064 nm and CW 1064 nm laser-treated groups at 6 weeks (logIgG) and maximal skin surface temperature (Tm) was not statistically significant; a Pearson's correlation coefficient r = −0.237 ( P = 0.08), where log(IgG) = −0.193Tm+10.75 (linear regression, R 2 = 0.056). n = 20, 17, 18, for OVA i.d. + PW 532 nm 1–4 minutes, OVA i.d. + PW 1064 nm 1–4 minutes, OVA i.d. + CW 1064 nm 1–4 minutes.
Kapa Library Amplification Kits, supplied by Roche, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/kapa library amplification kits/product/Roche
Average 96 stars, based on 1 article reviews
kapa library amplification kits - by Bioz Stars, 2026-04
96/100 stars
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Image Search Results


A, Serum ovalbumin- (OVA)-specific IgG titers 3, 6, and 12 weeks following vaccination with 40 µg OVA with or without laser illumination. Endpoint titer of OVA-specific serum IgG was determined by ELISA. Plates were coated with OVA. n = 33, 19, 15, 12 and 11, 5 and 6, 6 and 6 for no OVA, OVA i.d., OVA +Alum i.d., OVA i.d. + PW 532 nm 1 and 4 minutes, OVA i.d. + PW 1064 nm 1 and 4 minutes, OVA i.d. + CW 1064 nm 1 and 4 minutes, respectively. Error bars show means ± s.e.m. * P <0.05, ** P <0.01 and *** P <0.001 as compared to OVA i.d. B, The relationships between anti-OVA antibody titers following 1–4 minutes PW 532 nm, PW 1064 nm and CW 1064 nm laser-treated groups at 6 weeks (logIgG) and maximal skin surface temperature (Tm) was not statistically significant; a Pearson's correlation coefficient r = −0.237 ( P = 0.08), where log(IgG) = −0.193Tm+10.75 (linear regression, R 2 = 0.056). n = 20, 17, 18, for OVA i.d. + PW 532 nm 1–4 minutes, OVA i.d. + PW 1064 nm 1–4 minutes, OVA i.d. + CW 1064 nm 1–4 minutes.

Journal: PLoS ONE

Article Title: Near-Infrared Laser Adjuvant for Influenza Vaccine

doi: 10.1371/journal.pone.0082899

Figure Lengend Snippet: A, Serum ovalbumin- (OVA)-specific IgG titers 3, 6, and 12 weeks following vaccination with 40 µg OVA with or without laser illumination. Endpoint titer of OVA-specific serum IgG was determined by ELISA. Plates were coated with OVA. n = 33, 19, 15, 12 and 11, 5 and 6, 6 and 6 for no OVA, OVA i.d., OVA +Alum i.d., OVA i.d. + PW 532 nm 1 and 4 minutes, OVA i.d. + PW 1064 nm 1 and 4 minutes, OVA i.d. + CW 1064 nm 1 and 4 minutes, respectively. Error bars show means ± s.e.m. * P <0.05, ** P <0.01 and *** P <0.001 as compared to OVA i.d. B, The relationships between anti-OVA antibody titers following 1–4 minutes PW 532 nm, PW 1064 nm and CW 1064 nm laser-treated groups at 6 weeks (logIgG) and maximal skin surface temperature (Tm) was not statistically significant; a Pearson's correlation coefficient r = −0.237 ( P = 0.08), where log(IgG) = −0.193Tm+10.75 (linear regression, R 2 = 0.056). n = 20, 17, 18, for OVA i.d. + PW 532 nm 1–4 minutes, OVA i.d. + PW 1064 nm 1–4 minutes, OVA i.d. + CW 1064 nm 1–4 minutes.

Article Snippet: In the case of IgE, the wells were further treated with ELAST ELISA Amplification System (Perkin Elmer) to improve sensitivity of the assay.

Techniques: Enzyme-linked Immunosorbent Assay

A–G, Influenza-specific IgG subclass titers (A–C) in pre-challenge serum (4 weeks after vaccination) and (D–G) post-challenge (4 days after challenge). Mice were vaccinated with 1 µg of inactivated influenza virus (A/PR/8/34) with or without laser illumination or the licensed chemical adjuvant (alum) and challenged intranasally with live homologous virus 4 weeks after vaccination. Titer of influenza-specific serum IgG subclass was determined by ELISA. Plates were coated with inactivated influenza virus. (A and D) IgG, (B and E) IgG1, (C and F) IgG2c, and (G) IgE titers. Experimental and control groups: (A–C) n = 38, 47, 25, 50, 12 (D–F) n = 25, 29, 12, 32, 12 (G) n = 24, 21, 13, 24, 12 for no vaccine, vaccine i.d., vaccine i.d. + PW 532 nm, vaccine i.d. + CW 1064 nm, and vaccine + Alum i.d. vaccine groups, respectively.

Journal: PLoS ONE

Article Title: Near-Infrared Laser Adjuvant for Influenza Vaccine

doi: 10.1371/journal.pone.0082899

Figure Lengend Snippet: A–G, Influenza-specific IgG subclass titers (A–C) in pre-challenge serum (4 weeks after vaccination) and (D–G) post-challenge (4 days after challenge). Mice were vaccinated with 1 µg of inactivated influenza virus (A/PR/8/34) with or without laser illumination or the licensed chemical adjuvant (alum) and challenged intranasally with live homologous virus 4 weeks after vaccination. Titer of influenza-specific serum IgG subclass was determined by ELISA. Plates were coated with inactivated influenza virus. (A and D) IgG, (B and E) IgG1, (C and F) IgG2c, and (G) IgE titers. Experimental and control groups: (A–C) n = 38, 47, 25, 50, 12 (D–F) n = 25, 29, 12, 32, 12 (G) n = 24, 21, 13, 24, 12 for no vaccine, vaccine i.d., vaccine i.d. + PW 532 nm, vaccine i.d. + CW 1064 nm, and vaccine + Alum i.d. vaccine groups, respectively.

Article Snippet: In the case of IgE, the wells were further treated with ELAST ELISA Amplification System (Perkin Elmer) to improve sensitivity of the assay.

Techniques: Virus, Adjuvant, Enzyme-linked Immunosorbent Assay, Control