Journal: Redox Biology

Article Title: Adiponectin/AdiopR1 signaling prevents mitochondrial dysfunction and oxidative injury after traumatic brain injury in a SIRT3 dependent manner

doi: 10.1016/j.redox.2022.102390

Figure Lengend Snippet: Transcription and expressions of SIRT3 were regulated by AdipoR1/AMPK/PGC-1α signaling pathway in TBI models. (A–D) Western blot and statistical analysis of the phosphorylation of AMPK and expressions of SIRT3 and PGC-1α. (E) The transcription results of SIRT3 in AdipoR1 flox/flox and AdipoR1 CKO mice. **p < 0.01 and ***p < 0.001 vs sham group in each strain of mice, # p < 0.05 vs TBI group in each strain of mice, & p < 0.05, && p < 0.01, ns: no statistical significance. (F–I) Effects of AMPK phosphorylation inhibitor and AdipoRon treatment on AMPK phosphorylation and expressions of SIRT3 and PGC-1α after TBI. (J) Mechanism involved in protective effects of activated APN/AdipoR1 signaling after TBI. Data are presented as mean ± SD for n = 6. **p < 0.01 vs sham group, # p < 0.05 vs TBI group, $ p < 0.05 vs TBI + AdipoRon group.

Article Snippet: The membranes were immersed in 5% non-fat milk for 2 h and incubated at 4 °C overnight in the presence of primary rabbit polyclonal antibodies against Bcl-2 (12,789, Proteintech), Bax (50,599, Proteintech), AMPK (66,536, Proteintech), p-AMPK (2537, CST), PGC-1α (66,369, Proteintech), SIRT3 (10,099, Proteintech), PRDX3 (ab264354, Abcam), acetylated-Lysine (9441, CST), VDAC (55,259, Proteintech), and β-actin (AC026, ABclonal).

Techniques: Western Blot, Phospho-proteomics

Journal: Drug Design, Development and Therapy

Article Title: Cyanidin-3-O-Glucoside Improves Colonic Motility During Severe Acute Pancreatitis by Inhibiting the H 2 S-Regulated AMPK/mTOR Pathway

doi: 10.2147/DDDT.S256450

Figure Lengend Snippet: C3G administration suppressed CSE expression and deactivated the AMPK/mTOR pathway. *P<0.05 vs sham group; # P<0.05 vs SAP group. Abbreviations : C3G, cyanidin-3-O-glucoside; SAP, severe acute pancreatitis; CSE, cystathionine-γ-lyase.

Article Snippet: Antibodies against CSE (cat. no. 12,217-1-AP) were purchased from ProteinTech Group, Inc. Antibodies against total (t)-AMPK (cat. no. ab32047), phosphorylated (p)-AMPK (cat. no. ab23875), total (t)-mTOR (cat. no. ab2732), p-mTOR (cat. no. ab109268) and GAPDH (cat. no. ab181602) were purchased from Abcam.

Techniques: Expressing

Journal: Drug Design, Development and Therapy

Article Title: Cyanidin-3-O-Glucoside Improves Colonic Motility During Severe Acute Pancreatitis by Inhibiting the H 2 S-Regulated AMPK/mTOR Pathway

doi: 10.2147/DDDT.S256450

Figure Lengend Snippet: AMPK activation offset the effect of C3G on H 2 S-treated CMCs. ( A ) CSE levels and AMPK activity, ( B ) TNF-α levels, ( C ) serum IL-6 levels, ( D ) Ca 2+ accumulation. & P<0.05 vs CMC + H 2 S + C3G group.

Article Snippet: Antibodies against CSE (cat. no. 12,217-1-AP) were purchased from ProteinTech Group, Inc. Antibodies against total (t)-AMPK (cat. no. ab32047), phosphorylated (p)-AMPK (cat. no. ab23875), total (t)-mTOR (cat. no. ab2732), p-mTOR (cat. no. ab109268) and GAPDH (cat. no. ab181602) were purchased from Abcam.

Techniques: Activation Assay, Activity Assay

Journal: Journal of Medicinal Chemistry

Article Title: Optimization of Selectivity and Pharmacokinetic Properties of Salt-Inducible Kinase Inhibitors that Led to the Discovery of Pan-SIK Inhibitor GLPG3312

doi: 10.1021/acs.jmedchem.3c01428

Figure Lengend Snippet: Potency and Selectivity of SIK Inhibitors

Article Snippet: Homo sapiens SIK1 (full length, reference 02-131), ALK5 (catalytic domain aa200-503, reference 09-141), AMPKα1/β2/γ1 (full length, reference 02-147), LynA (full length, reference 08-171), and TGFβR2 (catalytic domain aa194-567, reference 09-142) were purchased from Carna Biosciences, DE.

Techniques: Inhibition, Binding Assay

Journal: Journal of Medicinal Chemistry

Article Title: Optimization of Selectivity and Pharmacokinetic Properties of Salt-Inducible Kinase Inhibitors that Led to the Discovery of Pan-SIK Inhibitor GLPG3312

doi: 10.1021/acs.jmedchem.3c01428

Figure Lengend Snippet: Conditions for Radioactive Kinase Assays

Article Snippet: Homo sapiens SIK1 (full length, reference 02-131), ALK5 (catalytic domain aa200-503, reference 09-141), AMPKα1/β2/γ1 (full length, reference 02-147), LynA (full length, reference 08-171), and TGFβR2 (catalytic domain aa194-567, reference 09-142) were purchased from Carna Biosciences, DE.

Techniques: Concentration Assay

Journal: iScience

Article Title: Activation of AMP-activated protein kinase (AMPK) through inhibiting interaction with prohibitins

doi: 10.1016/j.isci.2023.106293

Figure Lengend Snippet:

Article Snippet: Active recombinant human AMPK α1β1γ1 , Carna Biosciences , Cat#02-113.

Techniques: Produced, FLAG-tag, Control, Recombinant, Membrane, Transfection, Reverse Transcription, Silver Staining, Isolation, Cell Culture, Expressing, Plasmid Preparation, Luciferase, shRNA, Software