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Image Search Results
Journal: Pharmaceuticals
Article Title: Activation of AMPK/mTOR-Driven Autophagy and Suppression of the HMGB1/TLR4 Pathway with Pentoxifylline Attenuates Doxorubicin-Induced Hepatic Injury in Rats
doi: 10.3390/ph17060681
Figure Lengend Snippet: Pentoxifylline stimulated hepatic adenosine-monophosphate-activated protein kinase/mechanistic target of rapamycin (AMPK/mTOR) pathway in doxorubicin-intoxicated rats. Pentoxifylline (PTX; 100 mg/kg) was orally received daily, while doxorubicin (DOX; 18 mg/kg total cumulative dose; 3 mg/kg dose weekly) was received by the intraperitoneal route. The treatments were administered for 6 weeks. Herein, the protein expression of hepatic p-AMPK(Ser487), total AMPK, p-mTOR(Ser2448), and total mTOR was determined by ELISA. In the hepatic tissue of DOX-intoxicated rats, PTX resulted in an elevated p-AMPK(Ser487)/AMPK ratio ( A ) and diminished p-mTOR(Ser2448)/mTOR ratio ( B ). For n = 6, the values were shown as mean ± S.E.M. * p < 0.05, *** p < 0.001, or **** p < 0.0001 vs. control; # p < 0.05, ## p < 0.01 vs. DOX group. PTX, pentoxifylline; DOX, doxorubicin.
Article Snippet: The hepatic content of p-AMPK and total AMPK was measured using a
Techniques: Expressing, Enzyme-linked Immunosorbent Assay